Chemiric Antibody Production Service
Alpha Lifetech offer a wide range of unique services, including antibody humanization, expression of full-length chimeric antibody.
CONTACT USChemiric Antibody Production Service
As one of the professional bio-manufacturers, Alpha Lifetech Inc. is becoming an expert in the field of antibody engineering. We offer a wide range of unique services, including antibody humanization, expression of full-length chimeric antibodies, and large-scale production of single-domain antibodies (sdAbs).
Alpha Lifetech Inc. has developed a proprietary strategy for the rapid production of chimeric antibodies including full-length rat/human, mouse/human, mouse/rabbit, rat/rabbit, and other hybrid antibodies. Our proprietary chimeric antibody technology allows you to specify your requirements for expression and characterization. Our scientists have extensive experience in constructing and expressing recombinant or chimeric antibodies of various isotypes, including modified constant regions. We also specialize in expression in mammalian cells using our recombinant mammalian expression systems.
Introduction To Chimeric Antibody
Chimeric antibodies are antibodies that combine parental antibody variable regions and any acceptor constant area. Chimerization does not affect antibody specificity or affinity because of the way antibodies are organized. Chimeric antibodies retain the antigen specificity and affinity of the parental antibody but exhibit a longer half-life and greater ability to mediate CDC and ADCC responses.
A chimeric antibody (cAb) is an antibody made by fusing the antigen binding region (variable domains of the heavy and light chains, VH and VL) from one species like a mouse, with the constant domain (effector region) from another species such as a rabbit. Chimeric antibodies retain the antigen specificity and affinity of the original antibody but reduce the risk of reaction to foreign antibodies from a non-SELF source when used in the clinic. The chimeric antibodies are valuable tools for in vitro and in vivo research.
No restrictions on antibody types
Chimeric antibodies allow the antibody to be tailored to a specific application without being limited by the original antibody species
Adapt to any platform
Chimeric antibodies enable researchers to flexibly adapt their chosen monoclonal antibodies to any platform while maintaining the specificity and sensitivity of the original antibodies to specific antigens
Flow Cytometry and IHC
Chimeric antibodies can circumvent potential non-specific binding issues associated with secondary antibody binding to the test cell or tissue (a common problem for flow cytometry and IHC).
Antibody Humanization
Antibody chimerism is a preliminary step before antibody humanization, aimed at linking biological functions with parent antibodies and reducing their immunogenicity. Alternatively, chimeric antibodies can be used for therapeutic purposes in specific diseases or certain conditions (with high GI variable domains).
diagnostic purposes
Chimeric antibodies are the best substitute for serum, calibration standards, and positive controls in diagnostic analysis. They are particularly suitable because they can be produced in unlimited quantities and are uniform (maintaining antibody specificity and affinity).
assay development
Use as calibrators (standards) or controls in immunoassays - Serological immunoassays have been designed to detect a specific antibody in patient samples rely on serum calibrators (standards) and or positive controls
2018-07-16
