Fab Antibody Discovery Service

Alpha Lifetech Inc. is capable of providing different species or different isotypes of native or synthesized Fab antibody libraries based on our mature phage display technology platform. Building up a high-quality antibody library with a large library size and high diversity has been crucial for the successful isolation of antibodies, it will result in the speed of antibody-drug discovery. Now Alpha Lifetech's antibody discovery R&D team is proud to announce that we can develop a synthesized Fab library with independent clones over 10^11.

What Is Fab Antibody?

The IgG molecule can be subdivided into two functional subunits: (1) the fragment crystallizable (Fc), which constitutes the tail of the antibody and interacts with cell surface receptors to activate an immune response, and (2) the fragment-antigen binding (Fab), which mediates antigen recognition. The Fc region comprises two pairs of constant domains (CH2 and CH3) from two paired heavy chains, whereas the Fab region of the antibody consists of a variable domain followed by a constant domain from the heavy chain (VH and CH, respectively), which pair with a variable and constant domain from the light chain (VL and CL, respectively).

Fab Antibody Development-Phage Display Technology

Fab libraries, in which light-chain (LC) and heavy-chain (HC) variable region genes are cloned into a phagemid vector and subsequently displayed on the surface of the filamentous phage particle, have been widely used for the isolation of antibodies (Abs) with specificity for haptens, foreign antigens (Ags), and self Ags.
Alpha Lifetech can provide native Fab library construction and screening service and synthetic Fab library construction and screening service.

Why Choose Us?

● Vector Modification
Our scientists have also modified a vector system that has several features, such as different leader sequences for the light and heavy chains, a stop codon that allows easy shuttling between appropriate host strains for the preparation of phage or the expression of soluble Fab, a Myc tag for analysis and purification of the protein, and a subtilisin cleavage site useful for recovery of bound phage during library screening.

● Cloning Strategy
In order to construct a larger size antibody library, Alpha Lifetech Inc. utilizes an efficient cloning strategy for the construction of antibody phage libraries based on the isolation of restriction fragments from plasmid vectors instead of PCR products.

● One-Stop Service
With a deep understanding of the technology of antibody phage display library, we can tailor your project to one-stop construction & screening of antibody libraries, assist customers with any upcoming and problems in library construction and screening.

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