Aptamer Development Platform
ʻO nā Aptamers kahi oligonucleotide i hoʻopaʻa ʻia (DNA, RNA a i ʻole XNA) me ka waiwai o ka pilina kiʻekiʻe a me ka kikoʻī kiʻekiʻe e hoʻopaʻa pono ai i nā molekele e like me nā antibodies, a ua hoʻohana nui ʻia lākou no ka hoʻomohala ʻana i nā biosensors, diagnostics a me therapeutics.
ʻO ka aptamer platform i hāʻawi ʻia e Alpha Lifetech he ʻelua ʻāpana: aptamer synthesis platform, ka mea nui e pili ana i ka SELEX aptamer library synthesis service a me ka lawelawe hoʻomohala aptamer (DNA, RNA a i ʻole XNA), a me ka platform screening aptamer me nā lawelawe screening e pili ana i ka ʻenehana SELEX no nā protein, peptides, cell, nā mole liʻiliʻi, nā ion metala a me nā molekele i lalo. lawelawe.
Aptamer Synthesis Platform
ʻO ka lawelawe synthesis hale waihona puke ʻo SELEX aptamer
ʻO ka lawelawe ʻo SELEX aptamer library synthesis ka mea nui i ke kūkulu ʻana i kahi waihona i loaʻa ka nui o nā kaʻina oligonucleotide stranded single-stranded ma o ka synthesis kemika in vitro e like me nā molekula i loaʻa. ʻO ke kūkulu hale waihona puke ka hoʻomaka ʻana o ka ʻenehana SELEX, e hāʻawi ana i nā kaʻina moho he nui no ke kaʻina hoʻopaʻa ʻana ma hope o ke kūkulu ʻana i nā hale waihona puke nui a hoʻonui i ka hiki ke nānā i nā aptamers pili kiʻekiʻe.
Hoʻokaʻawale nui ʻia ka synthesis o ka waihona i nā pae aʻe:
ʻanuʻu | Nā kikoʻī ʻenehana |
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E ʻike i nā Molecules Target | E hoʻomaopopo i nā molekele i hoʻopaʻa ʻia e pono ke nānā ʻia no nā aptamers, hiki ke lilo i mau protein, nucleic acids, liʻiliʻi molekala, metala ion, etc. |
Hoʻolālā Kaʻina Kūlana | Ua hoʻolālā ʻia ka lōʻihi o ke kaʻina hana, ka haku mele a me nā ʻāpana ʻē aʻe e like me nā hiʻohiʻona o nā molekele i manaʻo ʻia a me nā koi screening. ʻO ka maʻamau, aia nā kaʻina maʻamau ma waena o nā ʻumi a me nā haneli kumu i ka lōʻihi. |
Hoʻohui ʻia o nā Kaʻina Paʻa | Hoʻolālā ʻia nā ʻāpana oligonucleotide me nā kaʻina paʻa (e like me ka PCR primer sequences) ma nā ʻaoʻao ʻelua i hoʻolālā ʻia a hoʻohui ʻia, e hoʻohana ʻia i ka hana hoʻonui a me ka nānā ʻana. |
Pono e hoʻoponopono hou ʻia ka waihona synthesized no ka mālama ʻana i ka maikaʻi. Ua hoʻoholo ʻia ka manaʻo o ka hale waihona puke e hōʻoia i kona hoʻohana ʻana i ke kaʻina hana kānana ma hope. Ua hōʻoia ʻia ka ʻokoʻa a me ka pololei o nā kaʻina maʻamau i loko o ka hale waihona puke e ka hoʻonohonoho ʻana a me nā ʻano hana ʻē aʻe e hōʻoia i ka hoʻokō ʻana o ka maikaʻi o ka waihona i nā koi screening.
Ma o nā ʻanuʻu i luna, hiki ke hoʻohui ʻia kahi waihona aptamer SELEX kiʻekiʻe a ʻokoʻa loa, hiki ke hāʻawi i nā kaʻina moho he nui no ke kaʻina screening ma hope.
Nā lawelawe hoʻomohala Aptamer (DNA, RNA a i ʻole XNA)
'Ōlelo pinepine nā Aptamers i nā aptamers nucleic acid. Hoʻokomo ʻia nā aptamers nucleic acid i nā aptamers DNA, RNA aptamers, a me XNA aptamers i hoʻololi ʻia i nā aptamers nucleic acid. Hoʻohana nui ʻia ka ʻenehana SELEX no ka hoʻomohala ʻana i nā aptamers. ʻO ke kaʻina hana maʻamau o nā lawelawe hoʻomohala aptamer e pili ana i ke kūkulu hale waihona puke, ka hoʻopaʻa ʻana i ka pahuhopu, ka hoʻokaʻawale ʻana a me ka hoʻomaʻemaʻe ʻana, ka hoʻonui ʻana, ka nui o ka nānā ʻana, a me ka ʻike ʻana. No nā makahiki he nui, ua kālele mākou i ka hana hale waihona puke a me ka ʻike waiwai i ka hoʻomohala ʻana i ka aptamer. Kūpaʻa mākou i ka hāʻawi ʻana i ka mea kūʻai aku me nā lawelawe ʻoi aku ka maikaʻi.
Kaʻina Hana ʻenehana ELEX
ʻO ke kaʻina hana SELEX he nui nā pōʻai, ʻo kēlā me kēia me kēia mau ʻanuʻu koʻikoʻi:
Hale Waihona Puke a me ka Hoʻopaʻa Paʻa
Hoʻohui ʻia ka hale waihona nucleic acid i kūkulu ʻia me nā molekele i hoʻopaʻa ʻia (e like me nā protein, nā pūhui mole liʻiliʻi, a me nā mea ʻē aʻe), i loaʻa i nā kaʻina nucleic acid i loko o ka hale waihona ka manawa kūpono e hoʻopaʻa ai i nā molekala.
Hoʻokaʻawale ʻia nā Molecules Unbound
Hoʻokaʻawale ʻia nā kaʻina nucleic acid i hoʻopaʻa ʻole ʻia i ka molekele i hoʻopaʻa ʻia mai ka hui ʻana e nā ʻano kikoʻī e like me ka affinity chromatography, ka hoʻokaʻawale ʻana i ka bead magnetic, etc.
Hoʻonui i nā Molekule Paʻa
Hoʻonui ʻia ke kaʻina waikawa nucleic i hoʻopaʻa ʻia i kahi mole i hoʻopaʻa ʻia, me ka hoʻohana pinepine ʻana i ka ʻenehana polymerase chain reaction (PCR). No ke kaʻina nānā ʻana ma hope, e hoʻohana ʻia nā kaʻina i hoʻonui ʻia ma ke ʻano he waihona hoʻomaka.

Fig 1: Ke kaʻina hana hōʻike ʻana o ELEX
Aptamer Screening Platform
ʻO ka lawelawe nānā ʻana o Aptamer
Hāʻawi ʻo Alpha Lifetech i kahi ʻano like ʻole o nā lawelawe screening aptamer kūikawā e pili ana i nā ʻano hana SELEX no nā ʻano ʻano like ʻole o kāu mau mole.
Nā ʻano pahuhopu | Nā kikoʻī ʻenehana |
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ʻO ka nānā ʻana i ka Protein Aptamer e SELEX | ʻO ke kumu nui o ka nānā ʻana i ka protein aptamer ka nānā ʻana i nā aptamers hiki ke hoʻopaʻa paʻa i nā molekole protein. ʻOi aku ka maʻalahi o kēia mau aptamers i ka synthesize, ʻoi aku ka paʻa a me ka liʻiliʻi i nā mea kūlohelohe. |
Nānā ʻia ʻo Peptide Aptamer e SELEX | ʻO nā peptide aptamers kahi papa o nā kaʻina peptide pōkole me ka kikoʻī kiʻekiʻe a me ka pili, hiki ke hoʻopaʻa pono i nā mea i hoʻopaʻa ʻia a hōʻike i kahi ākea o ka hiki ke hoʻohana ʻia i ka papa biomedical. Ma o ke kaʻina hana screening kikoʻī, ʻike ʻia nā peptide aptamers i hiki ke hoʻopaʻa paʻa i nā molekele i hoʻopaʻa ʻia mai ka nui o nā hale waihona puke peptide sequence. |
Hoʻopaʻa ʻia ʻo Aptamer kikoʻī o ke kelepona (Cell-SELEX) | Hoʻomākaukau ʻia nā pūnaewele a i ʻole nā molekole kikoʻī ma ka ʻili o ke kelepona e like me nā pahu hopu. Hiki i nā pahuhopu ke lilo i nā cell holoʻokoʻa, nā mea hoʻokipa ma ka membrane cell, nā protein, a i ʻole nā molekole liʻiliʻi ʻē aʻe. |
Nānā Molecule Aptamer Li'ili'i ma o Capture SELEX | ʻO Capture SELEX kahi ʻenehana nānā in vitro no ka nānā ʻana i nā aptamers mole liʻiliʻi, he ʻano like ʻole o SELEX. Ua kūpono loa ʻo Capture SELEX no ka nānā ʻana i ka aptamer o nā pahuhopu mole liʻiliʻi, ʻoi aku ka liʻiliʻi o nā pūʻulu hana a paʻakikī ke hoʻokaʻawale pololei ʻana i nā kākoʻo paʻa. |
Nā lawelawe SLEX e pili ana i nā holoholona | ʻO ka lawelawe ʻana i nā holoholona ola he ʻenehana hoʻokolohua i hoʻohana nui ʻia ma nā kula bioscience, lāʻau lapaʻau a me biotechnology, e hoʻohana ana i nā holoholona ola e like me nā hiʻohiʻona hoʻokolohua no ka nānā ʻana a loiloi i nā molekele kikoʻī, lāʻau lapaʻau, therapeutics a i ʻole kaʻina hana olaola. Hoʻolālā ʻia nā lawelawe e hoʻohālikelike i ke kaiapuni physiological i loko o ke kino kanaka e wānana pololei a loiloi i ka pono a me ka palekana o nā hopena hoʻokolohua i ke kino kanaka. |
ʻO ka lawelawe loiloi Aptamer
ʻO ka hydrophilicity, ka nalowale o ka pilina kiʻekiʻe i ka wā o ka hana ʻana, a me ka excretion wikiwiki o nā aptamers e kaupalena i kā lākou noi. I kēia manawa, ua ʻimi ʻia nā ʻano hana loiloi e hoʻomaikaʻi i ka hana o nā aptamers.
Loaʻa iā mākou nā ʻano ala like ʻole e hoʻomaikaʻi ai i ka aptamer e pili ana i ka ʻoki ʻana, nā hoʻololi, conjugation i ka hui kūpono (thiol, carboxy, amine, fluorophore, etc.).
ʻO ka lawelawe hōʻailona hōʻailona Aptamer
ʻO ka lawelawe aptamer characterization analysis e pili ana i ka lawelawe ʻoihana o ka hoʻonā ʻana o ka hoʻolālā loiloi hana a me ka hōʻoia hana o ka aptamer i loaʻa e hōʻoia i ka hoʻokō ʻana o ka aptamer i ka mana paʻa, kūpaʻa a me nā koi kikoʻī. Hoʻopili nui ia i ka pili a me ka nānā kikoʻī, ka loiloi kūpaʻa a me ka hōʻoia hana biological.
Inā he mau nīnau kāu, e ʻoluʻolu e kelepona mai iā mākou i kēlā me kēia manawa.
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