Protein
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Product Background
Catalog Number |
ALP64533 |
Product Name |
Recombinant Mouse Siglec-15/CD33L3 Protein, His Tag |
Synonyms |
CD33L3, CD33L3 protein, Mouse CD33L3 Protein, Recombinant Mouse CD33L3 Protein, protein CD33L3 antibody, Sialic acid-binding Ig-like lectin 15, Siglec-15, CD33 antigen-like 3 |
Alias |
Sialic acid-binding Ig-like lectin 15, Siglec-15 |
Official symbol |
CD33L3 |
Species |
Mouse |
Expression host |
HEK293 Cells |
Protein family |
Siglec Family |
Size |
50ug, 100ug |
Purification |
Affinity Purification |
Purity |
>90%-95% as determined by SDS-PAGE |
Endotoxin |
<1.0 EU/ug determined by the LAL method |
Buffer |
PBS, 150mM NaCl, 5% Thehalose, 0.3% Chaps, pH7.4 |
Storage |
For long term storage, store the reagent at -80°C. Avoid repeated freeze-thaw cycles. |
Shipping Condition |
Lyophilized powder is shipped at ambient temperature. Liquid form is shipped in dry ice. |
Note |
This product is for research use only. This product can not be used for human. |
FAQ
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What are some challenges associated with recombinant protein expression?
Challenges include protein misfolding, aggregation, low expression levels, toxicity to the host organism, and difficulties in achieving proper post-translational modifications. Optimization of expression conditions and selection of appropriate host organisms can help address these challenges.
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How can I purify the recombinant protein?
Protein purification methods vary depending on the protein's properties and the expression system used. Common techniques include affinity chromatography, ion exchange chromatography, size exclusion chromatography, and various forms of protein-specific purification methods (e.g., His-tag purification, GST-tag purification). Multiple purification steps may be required for obtaining highly pure proteins. Alpha Lifetech has multiple protein purification systems to help you obtain high-purity proteins.
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What are the common methods used for recombinant protein purification?
Common methods for protein purification include chromatography (e.g., affinity chromatography, ion exchange chromatography, size exclusion chromatography), precipitation, ultrafiltration, and dialysis. Each method exploits different physical or chemical properties of proteins to achieve purification.
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How do I choose the appropriate purification method for my protein?
The choice of purification method depends on various factors, including the size, charge, solubility, and specific properties of the target protein. Consideration should also be given to the scale of purification, the desired purity level, and the downstream applications of the purified protein.