Hybridoma Technology Platform
Hybridoma Technology

Antibody Drug Conjugate and Hybridoma Technology
hybridoma technology Production Antibody Service Workflow
Steps | Service Content | Timeline |
---|---|---|
Step 1: Animal immunization |
(1) Animal immunization 4 times, booster immunization 1 dose, a total of 5 doses immunized.
(2) Negative serum before immunization was collected, and ELISA was performed on the fourth dose to detect serum titer.
(3) If the serum antibody titer of the fourth dose meets the requirements, one additional dose of immunization will be administered 7 days before blood collection. If it does not meet the requirements, routine immunization will continue.
(4) Qualified potency, Spleen cells are isolated for cell fusion.
|
10-12 weeks |
Step 2: Fusion and Screening | (1) Fusion: After immunization, mouse spleen cells were collected and fused with myeloma cells.
(2) Proliferation and screening: The obtained hybridoma cells were subjected to proliferation and ELISA screening to obtain highly efficient clones.
|
6 weeks |
Step 3: Subclone | Subcloning 15-25 positive mother cells using limited dilution method, and finally determining 10-15 positive clones |
3-4 weeks |
Step 4: Ascites Production and Antibody Purification | (1) Ascites production: 10-15 hybridoma strains are used for ascites production in mice (if a large number of hybridoma strains are obtained, they will be sent in cellular form)
(2) Ascites identification: Ascites ELISA detection, potency (protein/virus>10 ^ 5, small molecule/peptide>10 ^ 4) for downstream purification
(3) Purification of Protein A/G: Purification of Ascites
|
3-4 weeks |
Step 5: Delivery |
(1) Provide 3-5 monoclonal antibody hybridoma strains.
(2) Each hybridoma cell provides 1mg of purified antibody (ascites purification).
(3) Experimental Process Report
|
1 week |

Multiple Species
Immunity can be provided to various animals, including but not limited to mice, rabbits, sheep, etc

Multiple Target
Based on hybridoma technology, multiple target antibody discovery services are available: proteins, peptides, small molecules, viruses, membrane proteins, mRNA, etc.

Traceability Records
QC quality control standards (immune potency, PBMC quality control, library quality control, and screening validation quality control), English experimental reports, original experimental records

Personalized Program
One on one personalized program customization to meet the research project needs of various clients
Monoclonal Antibody Development Service
We can provide high-quality, high-purity, and highly specific monoclonal antibody development services, including the production of mouse monoclonal antibodies and rabbit monoclonal antibodies
Hybridoma Technology Platform
Including immunization program, antibody preparation services, antibody purification, antibody high throughput sequencing, antibody validation, etc
Single B Cell Sorting Platform
Alpha Lifetech has advantages in screening time and obtaining high-quality antibodies. It can provide antigen design, synthesis, and modification, animal immunity, single B cell enrichment screening, single cell sequencing.