What are membrane proteins?

Membrane proteins are a diverse class of proteins that are embedded within or attached to the lipid bilayer of cell membranes. Membrane proteins can be divided into three classes: peripheral membrane proteins, lipid-anchored proteins, and integral membrane proteins. They play crucial roles in various cellular processes, including signal transduction, transport of molecules across membranes, cell-cell communication, and cell adhesion.

Conventional methods, such as the Mammalian System, Baculovirus-Insect System, Bacterial System, and Yeast System, for the preparation of integral membrane proteins have intrinsic limits in membrane proteins' expression, because the production of membrane proteins is a challenging task as their hydrophobic nature and their specific requirements in cellular expression systems frequently prevent an efficient synthesis. So far, a considerable number of membrane proteins from diverse families like prokaryotic small multidrug transporters (SMTs) or eukaryotic G-protein coupled receptors (GPCRs) have been produced in cell-free systems in high amounts and in functionally active forms.

Alpha Lifetech can offer?

Alpha Lifetech’s membrane protein expression service offers cutting-edge solutions for the production of challenging membrane proteins, including GPCRs, ion channels, and virus-like particles.

Workflow Of Membrane Protein Production