Membrane Protein Purification Service

Alpha Lifetech has successfully prepared thousands of recombinant proteins for our customers, including recombinant antibodies, secreted proteins, transmembrane proteins, proteases, etc., with high success rates. Customers only need to provide a protein sequence, CDS or protein name, and Alpha Lifetech can develop a complete fusion protein expression and purification plan in a shorter period of time. We can provide customized protein expression services based on the four major expression systems, and choose different fusion protein expression and purification strategies according to customer needs.

Alpha Lifetech has a variety of protein purification instruments and equipment, which can provide natural protein purification and recombinant protein purification services, such as affinity purification, molecular sieve chromatography, ion exchange chromatography, and hydrophobic chromatography. We will choose purification methods according to the specific needs of our customers, and combine them with our protein purification platform to obtain high-quality protein products in a short period of time.

Classification of Membrane Proteins

According to the distribution of proteins in the membrane and the relationship between membrane proteins and membrane lipids, membrane proteins are divided into three categories, including peripheral membrane proteins, integrated membrane proteins, and lipid anchoring proteins.

Ionic channel protein

Ionic channel protein is an integrated membrane protein that embeds into biological membranes to form specific channel structures, selectively allowing certain ions to be transported across membranes inside and outside the cell, while preventing other ions from passing through.The selectivity of ion channel proteins is crucial for maintaining cellular life functions such as the difference in ion concentration between inside and outside the cell, neurotransmitter transport, etc.

G protein coupled receptors

G protein coupled receptors are a general term for a large class of membrane protein receptors, which are the most numerous cell surface receptors in eukaryotes. The main function of G protein coupled receptors is to transmit extracellular information to the interior of cells through interaction with G protein, playing an important role in signal transduction. G protein coupled receptors recognize and bind to extracellular ligands to trigger intracellular signaling pathways, thereby regulating various physiological activities of cells.

Virus Like Particles

A virus like particle is a protein particle formed by the self-assembly of one or more structural proteins of a virus. These particles are similar in morphology and structure to natural virus particles, but do not contain the genetic material of the virus, therefore they are non infectious.

Virus Like Particles can serve as a display platform for membrane proteins, simulating the conformation and function of membrane proteins in natural environments by displaying specific membrane proteins on Virus Like Particles. In vaccine development, protective antigens of the virus, usually membrane proteins, can be displayed on Virus Like Particles to stimulate the body to produce immune responses against these antigens. Vaccines prepared through this method are highly safe and immunogenic.

Purification of Membrane Proteins

There are various methods for purifying membrane proteins, among which affinity chromatography utilizes the affinity of membrane proteins themselves or fusion tags, such as His tags, with their specific ligands to achieve membrane protein purification and separation. Ion exchange chromatography utilizes the charge properties of membrane proteins to separate them on ion exchange resins. However, membrane protein solutions often contain detergents, and resigning may affect the effectiveness of ion exchange chromatography. Gel filtration chromatography is to use the difference of membrane protein molecular weight to separate on the gel column, through this method, we can get high purity membrane protein. Due to the affinity and hydrophobicity of membrane proteins, they can be separated on chromatographic columns. This method is efficient, fast, and highly sensitive, but the cost is also relatively high.

membrane protein purification-Alpha Lifetech