Mice monoclonal Production Service

Alpha Lifetech Inc. has the ability to provide comprehensive services for scientists worldwide regarding mice hybridoma. These services include antigen design/synthesis, immunization, cell fusion, and hybridoma selection/characterization, and we can specialize in each project.

Mouse Mab Production Service- Hybridoma Technology

Antigen Preparation
● Peptide design & synthesis: peptide design, peptide synthesis, conjugation.
● Small molecule design & conjugation: Small molecule modification, conjugation.
● Protein preparation: cDNA synthesis, vector construction, protein expression & purification.
● Virus particles (Inactivated or attenuated).

Quality Control
Protein A/G purification
Antigen-specific affinity purification

Immunization
Immunized 4-5 mice (Complete Freund's adjuvant (CFA) and incomplete Freund's adjuvant (IFA) were injected subcutaneously on days 14, 21, 38, 52, and 68.)

Serum ELISA detection
ELISA titer>10^5 (Protein antigen, virus particles), ELISA titer>10^4 (Peptides or other molecules)

Fusion & Screening
Myeloma cells fused with splenocytes. The fusion of the B cells with myeloma cells can be done using electrofusion or PEG. Fused cells (B cells fused with myeloma cells) are incubated in HAT medium (hypoxanthine-aminopterin-thymidine medium) for roughly 2 weeks, since they have the hypoxanthine-guanine phosphoribosyltransferase (HGPRT) gene, making them non-sensitive and alive to the HAT medium. Screening is a rapid primary screening process, which identifies and selects only those hybridomas that produce antibodies of appropriate specificity.

Antibody validation
Our experienced team provides high-throughput hybridoma screening by ELISA, FACS, and other image-based binding methodologies (e.g., microbeads), as well as antibody biochemical characterization to ensure the identification of hybridoma candidates with high potency and to meet the special requirements of functional assays and animal studies.

Antibody production
Large-scale production of mouse monoclonal antibodies.

Why Choose Us? 

-- Picomolar affinity and high specificity

-- Ability to recognize subtle changes such as post-translational modifications

-- Cross-reactive antibodies recognizing human proteins and mouse orthologs

-- Excellent IHC results with formalin fixed paraffin embedded tissue

-- Ability to generate large panels of bioactive antibodies

-- Idea monoclonal antibody candidates for ADCC

-- Exceptionally high cell fusion rates (1-10 fusions/1,000 B-cells) for creation of hybridomas

-- High-throughput analysis of MAb pools (up to 50,000 samples/assay)

-- Wide array of in-vitro and in-vivo hybridoma screening and characterization assays

-- SPF animal facility
 
If you have any questions, please feel free to contact us at any time.