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Aptamer Development Platform

Pulatifomu ya aptamer yoperekedwa ndi Alpha Lifetech ikuphatikiza magulu awiri: nsanja ya aptamer synthesis ndi nsanja yowonera aptamer.

LUMIKIZANANI NAFE
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Aptamer Development Platform

Ma Aptamers ndi oligonucleotide yokhala ndi chingwe chimodzi (DNA, RNA kapena XNA) yokhala ndi kuyanjana kwakukulu komanso kutsimikizika kwakukulu komwe kumamangiriza ku mamolekyu monga ma antibodies, ndipo amagwiritsidwa ntchito kwambiri popanga ma biosensors, kuzindikira ndi kuchiza.

Pulatifomu ya aptamer yoperekedwa ndi Alpha Lifetech ili ndi magulu awiri: nsanja ya aptamer synthesis, yomwe makamaka imaphatikizapo ntchito ya SELEX aptamer library synthesis service ndi aptamer (DNA, RNA kapena XNA) ntchito yachitukuko, ndi nsanja yowunikira ma aptamer kuphatikiza ntchito zowunikira kutengera ukadaulo wa SELEX wamapuloteni, ma peptide, ma cell, mamolekyu ang'onoang'ono, ma molekyulu ang'onoang'ono, ma molekyulu achitsulo ndi ma molekyulu ena. ntchito zowunikira zizindikiritso.

Aptamer Synthesis Platform

SELEX aptamer library synthesis service

SELEX aptamer library synthesis service makamaka imaphatikizapo kupanga laibulale yomwe ili ndi mindandanda yambiri ya oligonucleotide yokhala ndi chingwe chimodzi mwa in vitro chemical synthesis molingana ndi mamolekyu omwe akuwafuna. Kumanga laibulale ndiye poyambira ukadaulo wa SELEX, womwe umapereka njira zambiri zowunikira popanga malaibulale akuluakulu osasinthika ndikuwonjezera mwayi wowunika ma aptamers ogwirizana kwambiri.
Kaphatikizidwe ka Library imagawidwa m'magawo awa:
Masitepe Zambiri Zamakono
Dziwani Zomwe Mukufuna Dziwani mamolekyu omwe amafunikira kuwunika ma aptamers, omwe amatha kukhala mapuloteni, ma nucleic acid, mamolekyulu ang'onoang'ono, ayoni achitsulo, ndi zina zambiri.
Random Sequence Design Utali wotsatizana mwachisawawa, kapangidwe ka maziko ndi magawo ena adapangidwa molingana ndi mawonekedwe a mamolekyu omwe akufuna komanso zowunikira. Nthawi zambiri, kutsatizana kwachisawawa kumakhala pakati pa magawo khumi ndi mazana ambiri kutalika.
Kuphatikizika kwa Sequences Zokhazikika
Zidutswa za oligonucleotide zokhala ndi zotsatizana zokhazikika (monga ma PCR primer sequences) pamapeto onse awiri amapangidwa ndikupangidwa, zomwe zidzagwiritsidwe ntchito pakukulitsa ndikuwunika kotsatira.
Laibulale yopangidwa ikufunikabe kukonzedwanso kuti iziwongolera bwino. Kuchuluka kwa laibulaleyi kunatsimikiziridwa kuti kuwonetsetse kuti ikugwiritsidwa ntchito poyang'ana ndondomeko yotsatira. Kusiyanasiyana ndi kulondola kwa kutsatizana kwachisawawa mu laibulale kunatsimikiziridwa ndi ndondomeko ndi njira zina zowonetsetsa kuti khalidwe la laibulale likukwaniritsa zofunikira zowunikira.
Kupyolera m'masitepe omwe ali pamwambapa, laibulale yapamwamba kwambiri komanso yosiyana siyana ya SELEX aptamer imatha kupangidwa, yomwe imatha kupereka zotsatizana zotsatizana nazo pakuwunika kotsatira.

Ntchito zachitukuko cha Aptamer (DNA, RNA kapena XNA)

Aptamers nthawi zambiri amatanthauza ma nucleic acid aptamers. Nucleic acid aptamers incutes DNA aptamers, RNA aptamers, ndi XNA aptamers omwe amasinthidwa mankhwala nucleic acid aptamers. Njira ya SELEX imagwiritsidwa ntchito kwambiri popanga ma aptamers. Mayendedwe oyambira a ntchito zachitukuko cha aptamer akuphatikiza kupanga laibulale, kumanga chandamale, kudzipatula ndi kuyeretsedwa, kukulitsa, kuwunika kangapo, ndikuzindikiritsa motsatizana. Kwa zaka zambiri, takhala tikuyang'ana kwambiri pakupanga laibulale komanso luso lolemera pakukula kwa aptamer. Ndife odzipereka kupatsa makasitomala ntchito zabwinoko.

SELEX Technology ndondomeko

Njira ya SELEX imakhala ndi zozungulira zingapo, iliyonse yomwe ili ndi masitepe otsatirawa:

Kumanga Library ndi Zolinga

Laibulale yopangidwa ndi nucleic acid imasakanizidwa ndi mamolekyu enieni (monga mapuloteni, mamolekyu ang'onoang'ono, ndi zina zotero), kotero kuti ma nucleic acid omwe ali mu laibulale ali ndi mwayi womanga mamolekyu omwe akuwafuna.

Kudzipatula kwa Mamolekyu Osamangidwa

Ma nucleic acid omwe samamangiriridwa ku molekyulu yomwe akufuna amasiyanitsidwa ndi kusakaniza ndi njira zina monga kuyanjana kwa chromatography, kupatukana kwa maginito, ndi zina.

Kukulitsa kwa Mamolekyu Omangirira

Kutsatira kwa nucleic acid kumangirizidwa ku molekyulu yomwe mukufuna kumakulitsa, nthawi zambiri pogwiritsa ntchito ukadaulo wa polymerase chain reaction (PCR). Pa gawo lotsatira lowunikira, zotsatizana zokulitsidwa zidzagwiritsidwa ntchito ngati laibulale yoyambira.
aptamer-Alpha Lifetech
Chithunzi 1: Njira yowunikira ya SELEX

Aptamer Screening Platform

Ntchito yowunikira aptamer

Alpha Lifetech imapereka ntchito zosiyanasiyana zowunikira ma aptamer omwe amagwiritsa ntchito njira zosiyanasiyana za SELEX zamitundu yosiyanasiyana ya mamolekyu anu:
Mitundu Yolinga Tsatanetsatane waukadaulo
Kuwunika kwa Protein Aptamer ndi SELEX Cholinga chachikulu pakuwunika kwa protein aptamer ndikuwunika ma aptamer omwe amatha kumangirira kuti agwirizane ndi mamolekyu a protein. Ma aptamers awa ndi osavuta kupanga, okhazikika komanso osakhudzidwa ndi chilengedwe.
Kuwunika kwa Peptide Aptamer ndi SELEX Ma peptide aptamers ndi gulu la ma peptide aafupi omwe ali ndi mawonekedwe apamwamba komanso ogwirizana, omwe amatha kumangirira kuzinthu zomwe amayang'ana ndikuwonetsa kuthekera kosiyanasiyana kogwiritsa ntchito pazachilengedwe. Kupyolera mu njira yowunikira, ma aptamers a peptide omwe amatha kumangirira ku mamolekyu omwe amayang'ana amawunikidwa kuchokera ku malaibulale ambiri otsatizana a peptide.
Kuwunika kwa Aptamer kwa Cell (Cell-SELEX) Maselo omwe amatsata kapena mamolekyu enaake pa cell amakonzedwa ngati chandamale. Zolinga zimatha kukhala ma cell athunthu, zolandilira pama cell membrane, mapuloteni, kapena mamolekyulu ena ang'onoang'ono.
Kuyang'ana kwa Molecule Aptamer yaying'ono ndi Capture SELEX Capture SELEX ndi njira yowunikira mu vitro yowunikira ma aptamers ang'onoang'ono a mamolekyu, omwe ndi mtundu wa SELEX. Capture SELEX ndiyoyenera kwambiri kuyang'ana ma aptamer a ma molekyulu ang'onoang'ono, omwe nthawi zambiri amakhala ndi magulu ochepa ogwira ntchito ndipo amakhala ovuta kusuntha mwachindunji pazothandizira gawo lolimba.
Live Animal-based SELEX services Ntchito yowunika potengera nyama ndi njira yoyesera yomwe imagwiritsidwa ntchito kwambiri pazasayansi, zamankhwala ndi sayansi yazachilengedwe, yomwe imagwiritsa ntchito nyama zamoyo monga zitsanzo zoyesera kuti ziwonetsere ndikuwunika mamolekyu ena, asing'anga, achire kapena njira zamoyo. Mautumikiwa adapangidwa kuti azitha kutsanzira chilengedwe m'thupi la munthu kuti athe kulosera molondola ndikuwunika mphamvu ndi chitetezo cha zotsatira zoyesera m'thupi la munthu.

Ntchito yokhathamiritsa ya Aptamer

Kuwonongeka kwa hydrophilicity, kutayika kogwirizana kwambiri panthawi yopanga, komanso kutulutsa mwachangu kwa ma aptamers kumachepetsa kugwiritsa ntchito kwawo. Pakalipano, njira zosiyanasiyana zokometsera zafufuzidwa kuti ziwongolere magwiridwe antchito a ma aptamers.
Tilinso ndi njira zosiyanasiyana zokometsera aptamer yopangidwa ndi kudulira, zosintha, kulumikizana ndi gulu loyenera (thiol, carboxy, amine, fluorophore, etc.).

Ntchito yowunikira mawonekedwe a Aptamer

Ntchito yowunikira mawonekedwe a Aptamer imatanthawuza ntchito yaukadaulo yowunikira magwiridwe antchito ndikutsimikizira magwiridwe antchito a aptamer yomwe yapezedwa kuwonetsetsa kuti aptamer ikukwaniritsa luso lomanga, kukhazikika komanso zofunikira zenizeni. Zimaphatikizanso kuwunika kwa kuyanjana ndi kutsimikizika, kuwunika kukhazikika komanso kutsimikizira kwachilengedwe.

Ngati muli ndi mafunso, chonde omasuka kulankhula nafe nthawi iliyonse.

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