Phage Display Antibody Development Platform
Phage display Technology

Phage Display Antibody Production Workflow
Steps | Service Content | Timeline |
---|---|---|
Step 1: Animal immunization |
(1) Animal immunization 4 times, booster immunization 1 dose, a total of 5 doses immunized.
(2) Negative serum before immunization was collected, and ELISA was performed on the fourth dose to detect serum titer.
(3) If the serum antibody titer of the fourth dose meets the requirements, one additional dose of immunization will be administered 7 days before blood collection. If it does not meet the requirements, routine immunization will continue.
(4) Qualified potency, blood collection and separation of monocytes
|
10 weeks |
Step 2: cDNA Preparation | (1) PBMC Total RNA Extraction (RNA Extraction Kit)
(2) High fidelity RT-PCR preparation of cDNA (reverse transcription kit)
|
1 day |
Step 3: Construction of Antibody Library | (1) Using cDNA as a template, genes were amplified by two rounds of PCR.
(2) Phage construction and transformation: gene splicing phagemid vector, electroporation transformation of TG1 host bacteria, construction of antibody library.
(3) Identification: Randomly select 24 clones, PCR identification positive rate+insertion rate.
(4) Assisted phage preparation: M13 phage amplification+purification.
(5) Phage display library rescue
|
3-4 weeks |
Step 4: Antibody Library Screening (3 rounds) | (1) Default 3-round screening (solid-phase screening): pressure screening to remove non-specific antibodies to the maximum extent possible.
(2) Single clone amplification bacteriophage selected+IPTG induced expression+ELISA detection of positive clones.
(3) All positive clones were selected for gene sequencing.
|
4-5 weeks |

Support Services
We can provide different animal based immune library construction services and natural antibody library screening services according to customer needs

Multiple Target
Multiple target antibody discovery services are available: proteins, peptides, small molecules, viruses, membrane proteins, mRNA, etc.

Multiple Vectors
Personalized library construction service, we can provide various bacteriophage vectors including PMECS, pComb3X, and pCANTAB 5E, and modify them according to customer needs

Mature Platform
Storage capacity can reach 10 ^ 8-10 ^ 9, insertion rates are all above 90%, and the affinity of antibodies obtained through screening is generally at the nM pM level
Monoclonal Antibody Development Service
We can provide high-quality, high-purity, and highly specific monoclonal antibody development services, including the production of mouse monoclonal antibodies and rabbit monoclonal antibodies
Hybridoma Technology Platform
Including immunization program, antibody preparation services, antibody purification, antibody high throughput sequencing, antibody validation, etc
Single B Cell Sorting Platform
Alpha Lifetech has advantages in screening time and obtaining high-quality antibodies. It can provide antigen design, synthesis, and modification, animal immunity, single B cell enrichment screening, single cell sequencing.

Phage Display Antibody Development Platform
Alpha Lifetech can provide phage display antibody development technical services from antibody preparation, antibody purification, antibody sequencing, etc.