Phage Display Peptide Library Construction Platform
Alpha Lifetech has a number of peptide library construction technologies. We mainly provide T7 peptide library construction service and M13 peptide library construction service according to phage types; we provide linear loop 7 peptide library construction and 12 peptide library construction service according to the sequence size of peptide molecules, etc. We also provide customized peptide library construction service. We also provide customized peptide synthesis and construction services.
Phage display technology as a technique for panning functional exogenous peptides in phage to the surface of the capsid, maintaining the structural and functional activity of the exogenous protein or peptide does not affect the ability of the recombinant phage to infect the host cell.Each phage viral particle can display only one exogenous random peptide on its surface, and the collection of all phages displaying different peptides constitutes a phage display peptide library. Phage display peptide libraries are powerful tools for the expression of multiple custom synthesized peptides and specific peptide screening, and have been widely used in the fields of antigenic epitope analysis, drug screening, and vaccine development.
Introduction to The Peptide Library Construction
Peptide library construction is to amplify peptide libraries by ligating peptide DNA sequences in different formats to the corresponding phage shell protein sequences, and using phage reassembly to pan the target peptide onto the phage surface. Peptide library construction based on phage display technology belongs to the biosynthesis category of peptide synthesis, and there are liquid-phase peptide synthesis and solid-phase peptide synthesis methods in the market. At present, Alpha Lifetech has a variety of peptide library construction technologies, which can provide pre-made peptide libraries and randomized peptide libraries according to the sequence size of the peptide molecules, including 7-peptide library construction, 12-peptide library construction, 15-peptide library construction, cyclic 7-peptide library construction, and cyclic 12-peptide library construction, and can also be customized to synthesize peptide libraries for a variety of types and applications.
Application of Peptide Library Construction and Screening
Randomized peptide libraries by phage display technology enable functional access to peptides and provide a physical link between phenotype (displayed peptide) and genotype (encoded DNA); these libraries are suitable for screening processes that separate bound clones from unbound clones by affinity purification. Phage-displayed peptide libraries can be used for (i) characterization of immune cellular epitopes (ii) screening of receptor- or protein-bound bioactive peptides, peptides bound to non-protein targets, cell- or organ-specific peptides, etc. and (iii) development of peptide-mediated drug delivery systems.
Phage Display Peptide Library Process

Fig.1 Phage Display Peptide Library Constrution Platform
Phage Display Peptide Library Construction Workflow
Process | Service Content | Timeline |
---|---|---|
1. Nucleotide Sequence Synthesis | Random synthesis of nucleotide sequences of customer specified length | 2-3 weeks |
2. Polymerization and Digestion | Polymerization and digestion of the synthesized DNA sequence | 1 day |
3. Electroconversion Libraries Construction | ER2738 host bacteria were constructed, and then E. coli was transformed with the well-constructed recombinant plasmid | 1 week |
4. Peptide Library Size and Diversity Detection | Detection of peptide library size by colony counting and peptide library diversity by sequencing | 2-3 days |
5. Phage Display Peptide Library Construction | By infecting the phage packaging peptide library | 1 week |

M13 Phage Display Peptide Library Construction Platform
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T7 Phage Display Peptide Library Construction Platform
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Phage display 7 peptide Library Construction Service
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Phage Display 12 peptide Library Construction Service
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M13 Phage Display Peptide Library Construction Service
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