Leave Your Message
slide1

Phage Display System

Alpha Lifetech has built a perfect stable Phage display system that saves time for scientific research time or project research and facilitates subsequent production

CONTACT US
01

Phage Display System

Alpha Lifetech has been deeply engaged in Phage display technology for many years. Alpha Lifetech has built a perfect stable Phage display technology platform that saves time for scientific research time or project research and facilitates subsequent production. We have multiple phage display systems available for customers to choose from, including M13 phage, T7 phage, T4 phage, and λ phage display systems. Depending on your experimental needs, you can select the appropriate phage display system. Alpha Lifetech can also provide customers services such as vhh antibody production, scfv antibody production, and Fab antibody production.

Introduction to Phage display

Phage display technology is a technique that uses phages (a virus that infects bacteria) to search for functional binding molecules of specific proteins or peptides. Phage antibody library construction techniques include Fab antibody antibody library construction, scFv antibody library construction, vhh antibody library construction, etc. According to the types of bacteriophages, they can be divided into bacteriophage m13, T7 bacteriophage, T4 bacteriophage, λ bacteriophage, and other bacteriophage . According to the library type, it can be divided into random peptide library, cDNA library, antibody library, and protein library. Phage display technology is simple to operate and inexpensive to use that contains Fab phage display, scfv phage display, vhh phage display. However, this technique limits the diversity of molecular genetics in the library that can't express too long sequences.

Phage display technology is capable of forming a physical link between the displayed molecule and the DNA sequence encoding the displayed molecule, and the amino acid sequence of a specific conjugate can be determined instantaneously by DNA sequencing of the desired fragment in the phage genome.
At present, phage display technology is widely used. Among them, there are significant achievements in the research and development of new vaccines (low-cost and efficient synthetic vaccines), the development of antibody drugs (screening enzyme inhibitors), cell signal transduction (screening of simulated epitopes), and the research of antigen epitopes (preparation of monoclonal antibodies).

Principle of phage display

Phage display is a molecular technology based on genetic modification of phage DNA, which binds the target fragment to the phage coat protein and thus expresses it on the phage surface. The foreign DNA sequence is introduced into a specific location in the nucleotide sequence of the phage genome that encodes a phage coat protein (Fig.1).
When a phage infection occurs, the phage gene begins to be expressed within the bacterial host, and the inserted target fragment is displayed on the phage surface as a combination of the related gene encoding the coat protein and the cloned sequence.
M13 phage
Fig.1 Bacteriophage M13 virion architecture and principle of the phage display technology. (Reference source: Phage display and other peptide display technologies | FEMS Microbiology Reviews | Oxford Academic (oup.com))

phage display Systems

Filamentous phages are the most commonly used phage display system, and phage display systems can be constructed using other phages. Such systems can display relatively large peptides or screen cDNA libraries more efficiently than filamentous phage-based systems.
m_fuab052fig10
Fig.2 Types of bacteriophages used in phage display. (Reference source: Phage display and other peptide display technologies | FEMS Microbiology Reviews | Oxford Academic (oup.com))

Advantage of Phage display system

Phage display systems usually employ filamentous phages, including M13, fd, and f1. Compared to M13 phages, T7 bacteriophage phages are composed of double-stranded DNA, which exhibits greater stability and is less prone to mutation during replication. T7 phage, as a carrier, grows quickly and can form plaques within 3h, which saves a lot of time for cloning and screening.
T7 phage library construction has been used for surface and non-surface gene expression and for constructing display libraries of peptides, cDNA, genomic DNA, and antibodies. This allows T7 phage to be used not only as an antimicrobial agent, but also for drug target screening in organ target screening (such as brain, liver, and lung), antibody recognition target screening, physiological mechanism research, etc. It can also be used for the diagnosis and treatment of various diseases.

Other forms of bacteriophages

Selectively infective phage

In contrast to traditional phage display, selective infective phage (SIP) links production protein-ligand interactions directly to phage infection and expansion without the need for elution steps.
m_fuab052fig8
Fig.3 Selectively infective phage (SIP). (Reference source: Phage display and other peptide display technologies | FEMS Microbiology Reviews | Oxford Academic (oup.com))

Landscape phages

Landscape phages are caused by thousands of copies of foreign peptides arranged around tubular capsids in super-tight repeating patterns, and the polyvalent display on landscape phages impels high-affinity selection, so it is impossible to distinguish between high and low affinity of peptide ligands.
m_fuab052fig7
Fig.4 The idea of landscape phages. (Reference source: Phage display and other peptide display technologies | FEMS Microbiology Reviews | Oxford Academic (oup.com))

Advantages of phage display systems

65e96cb083ae749122
65e96cafeceeb40761

Multiple phage display systems

M13 phage, T7 phage, T4 phage, λ phage display system for customers to choose from.
65e96cb083ae749122
65e96cafeceeb40761

 High throughput screening capability

We can use phage display systems to quickly construct large-scale phage libraries, and achieve high-throughput screening experiments.
65e96cb083ae749122
65e96cafeceeb40761

Customized phage display service

Customers can customize comprehensive services according to project requirements, such as screening high affinity antibodies, etc.
65e96cb083ae749122
65e96cafeceeb40761

Wide application range

Applied in fields such as antibody engineering, peptide and protein engineering, drug discovery and development, protein interaction research, etc.

Phage Display Systems

We have multiple phage display systems available for customers to choose from, including M13 phage, T7 phage, T4 phage, and λ phage display systems.

t7 phage display library-Alpha Lifetech
M13 phage display-Alpha Lifetech
λ phage-Alpha Lifetech
T4 phage-Alpha Lifetech
If you have any questions, please feel free to contact us at any time.

Leave Your Message

Featured Service