Platform Horumarinta Aptamer
Aptamers waa oligonucleotide-kaliya (DNA, RNA ama XNA) oo leh hanti xiriir sare leh iyo gaar gaar ah oo si gaar ah ugu xiraya molecules sida unugyada difaaca jirka, waxaana si weyn loogu isticmaalaa horumarinta biosensors, ogaanshaha iyo daaweynta.
Qalabka aptamer ee ay bixiso Alpha Lifetech waxaa ka mid ah laba qaybood: madal isku-darka aptamer, kaas oo inta badan ku lug leh adeegga isku-dhafka maktabadda ee SELEX aptamer iyo adeegga horumarinta aptamer (DNA, RNA ama XNA), iyo qalabka baaritaanka aptamer oo ay ku jiraan adeegyada baaritaanka ee ku salaysan tignoolajiyada SELEX ee borotiinnada, peptides, unugyada, molecules yar yar, hagaajinta birta iyo hagaajinta kale adeegyada falanqaynta aqoonsiga.
Platform Aptamer Synthesis Platform
SELEX adeega isku dhafka maktabadda aptamer
Adeegga isku-dhafka maktabadda SELEX aptamer wuxuu inta badan ku lug leeyahay dhisidda maktabad ka kooban tiro badan oo taxane oligonucleotide ah oo random-ka-soo-jeed ah iyadoo la raacayo isku-dhafka kiimikada ee vitro iyadoo loo eegayo moleculars bartilmaameedka. Dhismaha maktabaddu waa barta laga bilaabo tignoolajiyada SELEX, taas oo bixisa taxane musharraxeedyo badan oo ku saabsan habka baarista dambe iyadoo la dhisayo maktabado aan kala sooc lahayn oo kordhinaya suurtagalnimada baaritaanka aptamers-ku-xirnaanta sare.
Isku-dubaridka maktabadda waxaa inta badan loo qaybiyaa tillaabooyinka soo socda:
Talaabooyinka | Faahfaahinta Tignoolajiyada |
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Aqoonso Unugyada Bartilmaameedka | Aqoonso molecule-yada la beegsanayo ee u baahan in laga baadho aptamers, kuwaas oo noqon kara borotiinno, asiidhyo nuclei ah, unugyo yaryar, ion bir ah, iwm. |
Naqshad Taxane Random | Dhererka isku xigxiga ee aan tooska ahayn, halabuurka saldhigga iyo cabbirro kale ayaa loo qaabeeyey iyadoo loo eegayo sifooyinka molecules bartilmaameedka iyo shuruudaha baaritaanka. Caadi ahaan, isku xigxiga random-ku waxay u dhexeeyaan tobanaan iyo boqolaal saldhig oo dherer ah. |
Isku-darka Taxanaha Go'an | Jajabyada Oligonucleotide ee leh taxane go'an (sida taxanaha PCR premer) ee labada darafba waa la nashqadeeyey oo la farsameeyey, kuwaas oo loo isticmaali doono hab-weynaynta iyo baadhista xiga. |
Maktabadda la farsameeyey ayaa weli u baahan in si dheeraad ah loo habeeyo si loo ilaaliyo tayada. Isku-duubnaanta maktabadda ayaa la go'aamiyay si loo hubiyo in lagu dabaqi karo habka baaritaanka xiga. Kala duwanaanshaha iyo saxnaanta taxanaha random ee maktabadda waxaa lagu xaqiijiyay isku xigxiga iyo habab kale si loo hubiyo in tayada maktabaddu ay buuxisay shuruudaha baarista.
Iyada oo loo marayo tillaabooyinka kor ku xusan, maktabad SELEX aptamer tayo sare leh oo aad u kala duwan ayaa la soo saari karaa, taas oo bixin karta taxane musharraxeed oo badan habka baaritaanka xiga.
Adeegyada horumarinta Aptamer (DNA, RNA ama XNA)
Abtamers badanaa waxay tixraacaan atamerayaasha nucleic acid. Nucleic acid aptamers waxa ay ku jiraan aptamers DNA, RNA aptamers, iyo XNA aptamers kuwaas oo kiimiko ahaan wax laga beddelay aptamers nucleic acid. Farsamada SELEX waxaa si ballaaran loogu shaqeeyaa horumarinta aptamers. Socodka shaqada aasaasiga ah ee adeegyada horumarinta aptamer waxaa ka mid ah dhismaha maktabadda, ku-xidhka bartilmaameedka, gooni-u-goynta iyo nadiifinta, xoojinta, wareegyada kala duwan ee baaritaanka, iyo aqoonsiga isku xigxiga. Sannado badan, waxaan diiradda saarnay dhismaha maktabadda iyo waayo-aragnimada hodanka ah ee horumarinta aptamer. Waxaa naga go'an inaan siino macaamiisha adeegyo wanaagsan.
Habka Tignoolajiyada SELEX
Habka SELEX wuxuu ka kooban yahay wareegyo badan, mid kasta oo ka mid ah wuxuu ka kooban yahay tallaabooyinka muhiimka ah ee soo socda:
Laybareeriga iyo Isku xidhka Bartilmaameedka
Maktabadda aashitada nukliyeerka ah ee la dhisay waxaa lagu qasay unugyo bartilmaameed gaar ah (sida borotiinada, xeryahooda molecule yar, iwm.), si taxanaha aashitada nucleic ee maktabadda ay u helaan fursad ay ku xiraan molecules bartilmaameedka.
Go'doominta Molecules Unbound
Taxanaha aashitada nuclei-ka ee aan ku xidhnayn molecule bartilmaameedka ayaa laga soocaa isku dhafka iyadoo la adeegsanayo habab gaar ah sida chromatography affinity, kala-soocida birta birta, iwm.
Kordhinta Unugyada Isku Xidha
Taxanaha aashitada nukliyeerka ee ku xidhan molecule bartilmaameedka ah waa la xoojiyay, badanaa iyadoo la adeegsanayo tignoolajiyada silsiladda polymerase (PCR). Marxaladda baadhista ee xigta, taxanaha la xoojiyey ayaa loo isticmaali doonaa sidii maktabadda bilawga ah.

Sawirka 1: Habka baaritaanka SELEX
Platform Baaritaanka Aptamer
Adeegga baaritaanka Aptamer
Alpha Lifetech waxay bixisaa adeegyo kala duwan oo kala duwan oo ah baadhista aptamer-ka iyadoo adeegsanaysa habab kala duwan oo SELEX ah noocyada kala duwan ee unugyadaada:
Noocyada Bartilmaameedka | Faahfaahin Farsamo |
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Baaritaanka Protein Aptamer ee SELEX | Ujeedada ugu weyn ee baaritaanka abtamer borotiinka waa in la baaro atamerayaasha kuwaas oo si gaar ah ugu xidhi kara bartilmaameedka molecules borotiinka. Abtamers-yadani way ka fudud yihiin in la soo saaro, aad u deggan oo u nugul arrimaha deegaanka. |
Baaritaanka Peptide Aptamer ee SELEX | Peptide aptamers waa nooc ka mid ah taxanaha peptide-ga gaaban oo leh gaar gaar ah iyo xidhiidh sare, kaas oo si gaar ah ugu xidhi kara bartilmaameedka maaddooyinka oo muujiya tiro balaadhan oo ah suurtogalnimada codsiga ee goobta biomedical. Iyada oo loo marayo nidaam baadhiseed gaar ah, qalabka peptide ee si gaar ah ugu xidhi kara molecules-ka ayaa laga baadhay tiro badan oo ah maktabado isku xigxiga peptide random. |
Baaritaanka unugyada gaarka ah ee Abtamer (Cell-SELEX) | Unugyada bartilmaameedka ah ama unugyo gaar ah oo ku yaal dusha unugga ayaa loo diyaariyey sidii bartilmaameedyo. Bartilmaameedyadu waxay noqon karaan unugyo dhan, reseptors xuubka unugga, borotiinada, ama unugyo kale oo yaryar. |
Baadhitaanka Molecule Yaryar ee Abtamer ee Qabashada SELEX | Qabashada SELEX waa farsamo baadhiseed in vitro oo loogu talagalay baadhista aaladaha yaryar ee molecule, kaas oo ah nooc ka mid ah SELEX. Qabashada SELEX waxay si gaar ah ugu habboon tahay baadhista aptamer ee bartilmaameedyada molecule yar yar, kuwaas oo sida caadiga ah leh kooxo hawl yar oo ay adagtahay in si toos ah loo dhaqaajiyo taageerooyinka wejiga adag. |
Adeegyada SELEX ku salaysan Xoolaha nool | Adeegga baadhista xoolaha ku salaysan ee nool waa farsamo tijaabo ah oo si weyn loogu isticmaalo dhinacyada cilmiga noolaha, daawada iyo tignoolajiyada bayoolajiga, taas oo u adeegsata xayawaanka nool moodallo tijaabo ah si loo baadho oo loo qiimeeyo molecules gaar ah, dawooyinka, daaweynta ama hababka noolaha. Adeegyada waxaa loogu talagalay in lagu ekaysiiyo deegaanka jir ahaaneed ee jirka bini'aadamka si loo saadaaliyo si sax ah oo loo qiimeeyo waxtarka iyo badbaadada natiijooyinka tijaabada ah ee jirka bini'aadamka. |
Adeegga tayaynta Aptamer
Hydrophilicity-ka, luminta xidhiidhka sare inta lagu jiro wax soo saarka, iyo soo saarista degdega ah ee aptamers ayaa xaddidaysa codsigooda. Waqtigan xaadirka ah, habab kala duwan oo hagaajin ah ayaa la sahamiyay si kor loogu qaado waxqabadka aptamers.
Waxaan sidoo kale haysanaa siyaabo kala duwan oo loo hagaajiyo aptamer oo ka kooban goynta, wax ka beddelka, isku dhafka kooxda ku habboon (thiol, karboksi, amine, fluorophore, iwm.).
Adeegga falanqaynta sifada Aptamer
Adeegga falanqaynta sifada Abtamer waxa loola jeedaa adeega xirfada leh ee xallinta qaab dhismeedka qiimaynta waxqabadka iyo xaqiijinta shaqaynta ee abtamer-ka la helay si loo hubiyo in aptamer-ku uu buuxiyo awooda xidhidhiyaha gaarka ah, xasiloonida iyo shuruudaha gaarka ah. Waxaa inta badan ka mid ah xidhiidhka iyo falanqaynta gaar ahaaneed, qiimaynta xasiloonida iyo xaqiijinta shaqada noolaha.
Haddii aad wax su'aalo ah qabtid, fadlan xor u noqo inaad nala soo xiriirto wakhti kasta.
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