VHH Antibody Expression Service

Introduction Of VHH Antibody Expression Service

The immune system of camelids (llamas, alpacas and alpacas) is different from that of other animals in that their serum contains, in addition to the traditional IgG, an antibody to VHH that has lost its light chain.VHH is only 13-15 kDa in size, and is characterized by its small size, good stability, high solubility, and ability to be cleared from the bloodstream and penetrate deep into the tissues quickly, and it has a unique antigen-binding property compared to antibody fragments. VHH has been widely used in the field of antigen binding. Utilizing these advantages, VHH is widely used to become a biosensor, affinity capture, protein crystallization, molecular imaging, diagnosis and treatment of diseases.

VHH Antibody Expression Service Workflow

Steps	Specification	Timeline
Step1: Amplification/codon optimization and synthesis of the VHH gene	Using PCR, amplification of screened or synthesized VHH genes	2-3 weeks
Step2: Recombinant plasmid construction	Recombinant construction of the VHH gene in a suitable expression vector	2 days
Step3: VHH antibody expression	Mammalian Expression Systems E. coli Expression Expression System Rodent virus/insect cell protein expression Yeast Cell Expression Systems	2-3 weeks
Step4: Expression verification	Validation of expressed VHH proteins by SDS-PAGE, Western Blot, ELISA, immunoprecipitation, flow cytometry, or immunohistochemistry	2 days

What We Can Offer?

Protein Expression System	Dominance	Cell Line
Mammals expression system	Appropriate folding and post-translational modification of proteins is possible, and the protein structure and function are similar to those of natural proteins	Expi 293F, freestyle 293F, CHO-S, CHO-K1, 293T
E. coli expression expression system	Simple operation for large-scale preparation of proteins	BL21, Nissle 1917, Rosetta
Rodent virus/insect cell protein expression system	Multiple genes can be expressed simultaneously with high intracellular and secreted protein production	Sf9 cell, Sf21 cell, Tn-368 cell, and High Five cell
Yeast cell expression system	Capable of post-translational modification of proteins, including disulfide bond formation and glycosylation	GS115, X33

Alpha Lifetech provides personalized phage antibody library construction, screening, expression and antibody purification strategies to meet the different needs of our customers. We can use various types of antigens to immunize alpacas and construct VHH immune libraries based on phage display technology according to the needs of our customers. Our VHH immune libraries are diversified and can effectively produce VHH with high affinity to the original antigen, and we keep pre-made natural immune libraries in our laboratory, which do not require immunization of animals and greatly shorten the preparation time of VHH. We can also construct VHH-based synthetic libraries and semi-synthetic libraries according to customer needs. We isolate peripheral blood mononuclear cells (PBMC) from alpacas, and clone the acquired VHH gene into phage display vectors, which can generate a huge library of VHH antibodies through reverse transcription and polymerase chain reaction. We utilize the antigens required by our customers, and this library undergoes multiple rounds of screening and enrichment to obtain VHH antibodies that meet the needs of our customers for research, diagnosis or therapy.

Alpha Lifetech has a comprehensive recombinant antibody expression platform that can be utilized for VHH expression using different protein expression systems according to customer needs. Mammalian expression systems are the first choice for protein expression in humans or other mammals and are most commonly used in scientific research. Mammalian expression systems can correctly and efficiently recognize the synthesis, processing and secretion signals of eukaryotic proteins, have structure and function similar to natural proteins, can be modified post-translationally such as glycosylation, phosphorylation and acetylation, and have simple expression and purification processes, making it easy for transfection of recombinant plasmids. However, mammalian expression systems for protein passages usually have the disadvantages of low yield and long preparation time. Alpha Lifetech has been focusing on the optimization and research of mammalian expression systems for many years, and can provide customers with high yield and good quality VHH expression services. We are good at transient and stable expression of VHH using HEK293 or CHO cell lines for protein.