VHH Antibody Functional Research Service

Introduction Of VHH Antibody Functional Research Service

VHH antibody is derived from camelids ( camel, llama, alpaca) and sharks, and contains only one heavy chain antibody variable region, which is the smallest antibody with binding ability, with a size of only 12-15 kDa.VHH has the advantages of small size, good water-solubility, high stability, high acid resistance, low immunogenicity, high blood clearance and high tissue permeability. Based on these properties, VHH is widely used in affinity capture, biosensing, molecular imaging, diagnosis and treatment of diseases, etc. VHH is usually derived from immunobanks or natural libraries, and specific VHH antibodies are obtained by phage display and screening techniques.VHH is easy to be mass-produced in E. coli, and it has unique antigen-binding properties compared with other recombinant antibodies.

Alpha Lifetech combines proven phage display and screening technologies with antibody expression and purification platforms to prepare large quantities of VHH antibodies in a short period of time as required by our customers. The VHH antibodies we prepare have all the antigen-binding ability of traditional antibodies. Up to now, we have prepared a large number of high-affinity and specific VHH antibodies against different targets such as cytokines, cell surface receptors, tumor antigens, viruses, bacteria, enzymes and toxins.

Alpha Lifetech customizes personalized antibody characterization and functional validation programs to ensure the effectiveness of the prepared VHH antibodies. We are committed to producing high-quality recombinant antibodies for our customers. Through phage display and screening and antibody expression and purification technologies, we prepare VHH antibodies that bind specifically to the corresponding antigens and that have the correct binding epitopes. In the process of VHH antibody preparation, we formulate different functional validation programs for antibodies according to the needs of our clients and provide real experimental data to ensure the validity of the antibodies delivered to our clients. The purpose of our VHH antibody validation is to assess the specificity, sensitivity and reproducibility of the antibody to specific target antigens, to ensure the reliability of the antibody and to enhance the accuracy of the experimental results. The use of unvalidated or inadequately validated antibodies may have serious consequences that are detrimental to scientific research. To ensure the validity of the prepared recombinant antibodies, the VHH antibodies are rigorously validated to ensure that the antibody specifically recognizes the antigen without cross-reacting with other molecules, and to ensure the transparency and reproducibility of the experimental data.

What We Can Offer? 

We validate the prepared VHH antibodies using common immunological experimental techniques to ensure that the antibodies have strong affinity and specificity. We also evaluate the antibodies and optimize experimental parameters to ensure confidence in the results. Alpha Lifetech validates the reproducibility, specificity, stability and affinity of antibodies through protein immunoblotting (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), enzyme-linked immunosorbent assay (ELISA), immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), peptide array, flow cytometric analysis (FC), and knockdown cell lines. , stability and affinity to ensure that the VHH antibodies we prepare meet our customers' needs. We validate the specificity of VHH antibodies by protein immunoblotting (WB) to ensure that they have a single band of the expected size; we use immunohistochemistry (IHC) to visualize protein expression in tissue samples, and we stain and localize tissue sections with VHH antibodies to verify that the antibodies bind specifically to the desired proteins in the tissues; and we also utilize RNA interference (RNAi ) or CRISPR/Cas9-mediated gene knockdown, among other technologies, to ensure that the VHH antibody is specific to the intended target by silencing or knocking down the target protein. We also provide services such as cellular value-addition or invasion assays to ensure the in vivo potency of the antibodies.