Alpha Lifetech offers 12-mer peptide library screening services for various targets such as antibodies, receptors, tissues, peptides and enzymes. We have developed 12-mer peptide libraries for solid-phase screening, liquid-phase screening, cell-based screening (tumor cells, primary cells), and in vivo screening in animals. The capacity of our 12-mer peptide library is up to 109, which makes it easier to screen the ideal peptide sequences due to the large library capacity. We validated the screened peptides and 98% of them have good specificity and affinity. We will go through 3 rounds of enrichment and screening of the constructed 12-mer peptide library, wash out the non-specific phage, and elute down the peptide phage that binds to the target protein. We will titer the elution products, obtain the blue and white spot plate and send it for sequencing. Finally, We analyze and deliver 10-20 peptide sequences according to customers' needs. To ensure the validity of the peptides, we can characterize the affinity of the screened 12-mer peptide, such as EC50 assay, BLI, ELISA and FACS. To ensure the functional integrity of the screened sequences, we will perform NGS validation on the screened sequences.

Fig.1 Dodecapeptide library screening process