Introduction to 5T4/TPBG

Drug targetis a biomolecule that can directly bind to drugs and then react. Generally, the target refers to protein, which is related to the cause of disease. 5T4/TPBG protein is a common target in medicine.

5T4/TPBG (also known as trophoblast cell glycoprotein or Wnt signaling regulator) is one of these clarity transmembrane proteins, 5T4/TPBG protein can have clarity functions in the embryonic development stage of living organisms, in medical cancer research, and in the development of drugs.5T4/TPBG, It is regulated by certain genes and belongs to the leucine repeat (LRR) protein family. 5T4/TPBG has a relatively variable morphological structure and function, which makes 5T4/TPBG become a focus of current research. The unique molecular structure 5T4/TPBG protein has a moderate molecular weight, consisting of more than 400 amino acids, of which 5% is the transmembrane region. The 5T4/TPBG molecular architecture contains a distinct extracellular domain, which can reconstruct the cytoskeleton and affect the cell-to-cell integrity through the PDZ domain of 5T4/TPBG. In addition, the outer part of 5T4/TPBG has some modifications, which make the protein more stable and also related to the biological activity of the protein. The transmembrane region and the intracellular tail simultaneously affect the localization of the tin to the membrane and the onset of a variety of different signals.

Function of Target 5T4/TPBG

Many scientists have shown that the 5T4/TPBG proteins have many different functions, including the molecular ones and the cellular ones. During biological embryonic development, 5T4/TPBG is a marker of early embryonic differentiation in the initial embryo, which affects and regulates the key processes of epithelial-stromal transformation (EMT), which are very important for the morphological formation of the embryo. However, in the tumor part, the abnormal expression of 5T4/TPBG protein is closely related to the progression, metastasis, and poor prognosis of several different malignancies, making 5T4/TPBG a relevant target for tumor diagnosis and treatment. The 5T4/TPBG proteins of the biological signaling pathway function mainly through regulating the Wnt signaling pathway. 5T4/TPBG protein, a negative regulator on the Wnt/β-catenin signaling pathway, can interact with other molecular discoveries in the signaling pathway and then inhibit the activation of classical Wnt signaling, which subsequently affects the proliferation, migration, and differentiation of body cells. At the same time, 5T4/TPBG proteins may also participate in regulating the internal environment of tumor cells and their interaction with other cells through other pathways, such as the CXCL12/CXCR 4 biological axis.

Fig.1 Gene Pathway. (Reference documentation:Stern PL, Brazzatti J, Sawan S, McGinn OJ. Understanding and exploiting 5T4 oncofoetal glycoprotein expression. Semin Cancer Biol. 2014 Dec;29:13-20.)

Alpha Lifetech Can Provide

As a multifunctional transmembrane glycoprotein, 5T4/TPBG protein has shown important research value and application prospects in embryonic development, tumor biology, and drug research and development. With further research and technological advances, 5T4/TPBG protein products are also essential. Alpha Lifetech can provide 5T4/TPBG corresponding products and help each customer's research and development. In addition, Alpha Lifetech can provide efficient and accurate EC50/IC50, helping customer companies to screen compounds efficiently and accurately. At the same time, Alpha Lifetech also provides a variety of phage services such as phage antibody library construction services, phage antibody library screening services, as well as whole chain services such as antibody customization and recombinant antibody purification.

Catalog Number	Product Name	Product Sizes
ADT1423	Naptumomab Estafenatox Biosimilar–Anti-TPBG mAb–Research Grade	1mg, 5mg
ALP64514	Recombinant Human 5T4 Protein, Avi Tag, His Tag	50ug, 100ug

Reference

[1]Stern PL, Brazzatti J, Sawan S, McGinn OJ. Understanding and exploiting 5T4 oncofoetal glycoprotein expression. Semin Cancer Biol. 2014 Dec;29:13-20. doi: 10.1016/j.semcancer.2014.07.004. Epub 2014 Jul 25. PMID: 25066861.

[2]Awan A, Lucic MR, Shaw DM, Sheppard F, Westwater C, Lyons SA, Stern PL. 5T4 interacts with TIP-2/GIPC, a PDZ protein, with implications for metastasis. Biochem Biophys Res Commun. 2002 Jan 25;290(3):1030-6.

[3]Harris JL, Dave K, Gorman J, Khanna KK. The breast cancer antigen 5T4 interacts with Rab11, and is a target and regulator of Rab11 mediated trafficking. Int J Biochem Cell Biol. 2018 Jun;99:28-37.