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Product Background
Catalog Number |
ADT1497 |
Product Name |
ADT1497-Pembrolizumab Biosimilar-Anti-PDCD1/PD1/CD279 mAb-Research Grade |
Alias |
MK-3475 |
Isotype |
IgG4 Kappa |
Clonity |
Monoclonal |
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Alternate Names anti-mouse pd-1 antibody, anti-pd-1 antibody, anti pd-1 therapy, anti pd-1 antibody, recombinant pd-1, pd-1 antibody, anti-pd-1 monoclonal antibody, CD279 antibody, anti CD279, anti-CD279 antibody, human CD279 antibody, recombinant CD279, CD279 recombinant antibody, CD279 blocking antibody |
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Official Symbol |
PDCD1/PD-1/CD279 |
Species |
Human |
Expression Host |
CHO Cells |
Reactivity |
Human |
CAS Number |
1374853-91-4 |
Chemical Formula |
C6504H10004N1716O2036S46 |
Molecular Weight |
146.27 KDa |
Product Description |
Pembrolizumab, as an IgG4 subclass antibody, is preferred over other subclasses as it only induces weakly the complement and cell activation due to low affinity to C1q and Fc receptors. It binds with high affinity to the cell surface receptor programmed cell death protein 1 and it antagonizes its interaction with its known ligands PD-L1 and PD-L2. In normal circumstances, the binding of the ligands of PD-1 to the receptor Inhibitionits the TCR-mediated T cell proliferation and cytokine production. This Inhibitionitory signal seems to be essential for self-tolerance, collateral damage minimizing after immune response against a pathogen and maternal tolerance to fetal tissue. Therefore, the binding of pembrolizumab to PD-1 prevents the Inhibitionitory pathway causing a physiological shift to immune reactivity and enhancing tumor immunosurveillance and anti-tumor immune response. |
Mechanism of Action |
Pembrolizumab is a monoclonal antibody that targets programmed cell death-1 (PD-1) receptor on T-cells.
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Metabolism |
Pembrolizumab is catalyzed into small peptides and single amino acids via general protein degradation but it does not rely on metabolism for clearance. |
Gene ID |
5133 |
Size |
100ug,1mg,5mg |
Research Area |
Cancer |
Application |
Elisa, WB |
Purity |
>95% as determined by SDS-PAGE |
Concentration |
Batch dependent |
Buffer |
Supplied in PBS, PH7.5 |
Storage |
Store at -20 °C for 12 months. Store at -80 °C for long term storage. |
Shipping Condition |
Shipped on ice packs. |
Note |
This product is for research use only. |
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Reference 1. Ma Y, et al. Zhonghua Yu Fang Yi Xue Za Zhi, 2023 Jun 6. PMID 37357205
2. Mirsharif ES, et al. Int Immunopharmacol, 2023 Jun. PMID 37172423
3. Tarique M, et al. Front Immunol, 2023. PMID 37153623
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FAQ
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What is a biosimilar?
A biosimilar is a biological product that is highly similar to an existing approved reference biological product (also known as the originator or reference product), with no clinically meaningful differences in terms of safety, purity, and potency. Biosimilars are developed to be highly similar to the reference product in terms of structure, function, pharmacokinetics, and clinical efficacy. Alpha Lifetech can provide biosimilar antibody products.
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How can I obtain COA of the reagent that I received?
Please contact us via email info@alphalifetech.com for detailed information about the product.
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Why choose Alpha Lifetech’s product?
Alpha Lifetech's products are chosen for their quality, reliability, and competitive pricing. Our biosimilar antibodies undergo thorough development and testing processes to ensure they meet regulatory requirements and deliver comparable performance to reference products. We prioritize customer satisfaction and strive to provide cost-effective solutions without compromising on quality. -
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How to purify your antibodies?
Protein A/G Chromatography: Protein A and Protein G are affinity chromatography matrices that specifically bind to the Fc region of antibodies, allowing for their purification from complex mixtures.
Ion Exchange Chromatography: Ion exchange chromatography separates proteins based on their net charge at a specific pH.
Size Exclusion Chromatography: Size exclusion chromatography (SEC), also known as gel filtration chromatography, separates proteins based on their size and shape.
Affinity Tag Purification: Some recombinant antibodies are engineered with affinity tags (e.g., His-tag, FLAG-tag) that facilitate purification. Affinity tag purification involves using specific affinity resins or columns that bind to the tag, allowing for purification of the tagged antibodies. Alpha Lifetech has multiple antibody purification strategies. Please contact us via email info@alphalifetech.com for more detailed information.