Bispecific Antibody Expression Platform

With the rapid development of the bio-industry, Alpha Lifetech follows the frontier of life science and technology, and offers bispecific antibody development for global customers, in order to prepare bsAbs with good therapeutic effects. With ten years of experience in antibody R&D, professional researchers and advanced equipment, we can support our customers with bispecific antibody construction, expression and purification services. We can construct IgG-like and non-IgG-like bsAbs to meet the different research needs of our global customers. We express bsAbs using various systems such as mammalian cells, yeast cells and E. coli. Mammalian cell expression systems have post-translational modification capabilities and are most commonly used for bispecific antibodies expression. We have highly efficient expression vectors, specific high quality media, transfection reagents and well-established cell culture techniques. Our mammalian cell lines include CHO and HEK293 cells, which can meet the different needs of our customers around the world. Alpha Lifetech offers antibody expression from microgram to gram scale, and can prepare large quantities of antibodies with high purity and low toxicity in a short period of time, which can greatly shorten the time and cost of research.

Introduction to Bispecific Antibody

Bispecific antibodies (bsAbs) are a new type of therapeutic antibody that can bind to two different antigens or epitopes at the same time with high specificity and targeting. bsAbs are structurally classified into IgG-like and non-IgG-like. IgG-like bispecific antibodies are similar in structure to natural immunoglobulins, consisting of two antigen-binding fragments (Fab) fused to the constant region of IgG molecules. This structure of bsAbs has the advantages of good stability, long serum half-life and good antibody effector function, and can be used for recruitment of immune cells and systemic administration. Non-IgG-like bsAbs consist of antibody-binding fragments such as VHH, scFv or Fab, and lack a stable Fc region. Due to their small molecular weight, low immunogenicity, strong tissue penetration ability and convenient preparation, non-IgG-like bsAbs are widely used in the field of disease treatment. Bispecific antibodies are more efficacious and less costly than the combination of two monoclonal antibodies, and have fewer side-effects than the combination of multiple monoclonal antibodies, so they are the most important antibody drugs that have been researched in the near future.

Fig. 1 Different forms of bispecific antibodies

Bispecific Antibodies Cancer treatment

Bispecific antibodies have broad application prospects in the treatment of tumors and infectious diseases, immune research and other fields. In tumor therapy, bsAbs bind to target receptors expressed on cancer cells and regulate various cellular activities such as proliferation, survival and apoptosis. bsAbs can be used in the treatment of tumors and infectious diseases. bsAbs can be used in the treatment of cancer and infectious diseases. Antibody-dependent cytotoxicity (ADCC) is the key to triggering an immune response against cancer cells. Bispecific antibodies can bind cancer cells to T-cells and bring the immune system into direct contact with the tumor, triggering a series of immune responses and promoting tumor cell lysis. bsAbs not only have good immune therapeutic efficacy against cancer, but also have a promising application in the treatment of Alzheimer's disease and other neurodegenerative diseases. In addition, bispecific antibodies have great research value in the field of drug delivery and ADC therapy research.

Bispecific Antibodies Purification

We purify bispecific antibodies using Ion Exchange Chromatography (IEC), Size-Exclusion Chromatography (SEC), and protein A/G/L affinity chromatography. bsAbs are validated using a range of ELISA, SPR, BLI, FACS, and cytotoxicity assays to ensure that they have good specificity, affinity, and functional integrity. Alpha Lifetech will optimize the expression conditions, stability, specificity and affinity of bsAbs. In addition, we will bsAbs half-life, pharmacokinetic evaluation, to ensure that it has a good disease therapeutic effect.

Bispecific Antibodies Expression Service Process

Our Advantages

We cover the upstream and downstream services of antibody production, and can provide technical services from antibody preparation, bispecific antibody purification and antibody separation and purification, antibody sequencing, antibody validation, etc, , which can be used for bispecific antibody therapy.

High Expression

High protein expression in CHO cells.
Stable Storage

>2 weeks in serum at 37 °C.
No Solubility Issue

>30 mg/ml
Stable Cell Lnes

Many stable cell lines with tumor antigens have been developed to validate many bispecific antibodies