Cyclic 7-mer Peptide Library Screening Service
Alpha Lifetech brings over a decade of experience in phage high throughput library screening.
Request Quote01
cyclic 7-mer peptide library screening service
Alpha Lifetech brings over a decade of experience in phage high throughput library screening. Leveraging our advanced phage display platform, we can screen high-affinity peptides quickly and efficiently for disease treatment and biological research. We offer a range of phage display peptide library construction services such as M13, T4, T7 or λ phages for our customers, with M13 phage display being our commonly used method. We are capable of constructing linear peptide libraries and structurally stable cyclic peptide libraries. Notably, We can create phage cyclic 7-mer peptide libraries with a library capacity up to 10^10 and enriched phage particles up reaching 10^13, which is conducive to screening specific antibodies with high affinity. We can tailor peptide libraries with different structures containing 5-25 amino acids. In addition to cyclic 7-mer peptide libraries, we also construct linear 7-mer peptide libraries, linear 12-mer peptide libraries, linear 15-mer peptide libraries, linear 9-mer peptide libraries and cyclic 9-mer peptide libraries. Different screening methods are suitable for different screening antigens.
Alpha Lifetech can develop customized screening solutions for customers to screen the constructed cyclic 7-mer peptide libraries with different targets, including proteins, antibodies, receptors, peptides or enzymes, tissue samples, and nucleic acids.
Alpha Lifetech is devoted to screening high affinity small molecule peptides for our clients and performing various validations for the peptides. We provide EC50 assays,BLI, SPR and other peptide affinity validation experiments, FACS, various cellular function validation and in vivo animal function validation. We execute blue-white screening, sequencing and peptide sequence analysis for phage following 3-5 rounds of panning. Alpha Lifetech provides peptide gene information, amino acid sequence information and sequencing raw data and project reports for all clients.
Background on Peptide Library Screening
Phage peptide library screening involves the fusion of exogenous peptide genes with phage coat proteins and displaying them on the phage surface. This technique facilitates obtaining target-binding peptides through multiple rounds of screening. Peptides are small, only 1-2 kDa, with specificity and affinity for the target. Their small size allows for excellent tissue penetration into tumors, while also minimizing toxicity, reactogenicity and side effects. Small peptides are more easily modified to increase affinity, making them widely used in clinical diagnostics. In addition, it has been shown that cyclized peptides are more stable and esistant to hydrolysis compared to their linear structures. Peptide library screening has found extensive applications in cancer therapy, drug discovery, vaccine development, antigenic epitope prediction, biomarker discovery and cell signaling pathway research, which presents a promising horizon for future biological research and disease treatment.
Peptide Library Screening Service Process
Peptide library screening based on phage display technology can be categorized into solid-phase screening, liquid-phase screening, whole-cell screening and in vivo screening. The screening process mainly includes (1) incubating the peptide library with the target; (2) washing away the unbound phage; (3) eluting the bound phage; and (4) enriching the eluted phage for subsequent screening. After 3-5 rounds of screening, phage enrichment and elution were analyzed using titer assay. Finally, individual phages were sequenced to obtain sequences with high affinity and specificity. In vivo screening involves screening phage libraries in live or freshly isolated tissues to isolate highly specific binding peptides. In order to fulfill the needs of our customers, researchers at Alpha Lifetech will find the most appropriate method for screening peptide libraries based on the specific circumstances. Liquid-phase screening is good for increasing the lexibility of antigens screening, and we utilize SA magnetic bead to solate high-affinity, high-specificity peptides. We use are good at using peptide libraries for in vivo screening. In this process, phage cyclic 7-mer peptide libraries are injected intravenously into animals such as rats.The screened phage will be enriched from the tissues, and multiple rounds of screening will be performed. Finally, the recovered phage clones will be sequenced and analyzed to identify the peptides of interest.
Fig.1 Cyclic Heptapeptide library screening process
cyclic 7-mer peptide library screening Workflow
| Process | Service Content | Timeline |
|---|---|---|
| 1. Peptide Gene Design | Design of peptides containing 5-15 amino acids. | 1-2 days |
| 2. Gene Amplification And Recombinant Plasmid Construction | Amplify genes and construct on phage vectors | 1-2 weeks |
| 3. Transformation | Transform recombinant plasmids into competent cells | 2 days |
| 4. Collection of Peptide Libraries and Validation | Perform insertion rate and diversity identification | 2 days |
| 5. Peptide Library Screening | Conduct screening using solid-phase screening, liquid-phase screening, whole-cell screening, in vivo rat screening | 2-3 weeks |
| 6. Sequencing | Perform sequencing to characterize the screened peptides | 4 days |
| 7. Peptide Validation | Conduct validation assays including EC50, SPR, BLI, ELISA, FACS, NGS, cellular function validation and in vivo animal validation | 1 week |
If you have any questions, please feel free to contact us at any time.
Leave Your Message
2018-07-16
0102