Fab Antibody Expression Service
Introduction of Fab Antibody Expression ServiceFab
Fab, also known as antigen-binding fragment, is a region in the antibody structure that can bind to antigen and consists of a complete light chain (variable and constant regions) and a partial heavy chain structure (variable region and a constant region fragment), with the light chain and the heavy chain connected by a disulfide bond.Fab is small in size, with a molecular weight of 47-48 kDa, and is usually prepared using phage display technology or mammalian cell expression systems. Fab can be used in cancer therapy to target specific tumor antigens or as probes for the detection of specific molecular markers, and is widely used in disease diagnosis and treatment and biological research. Fab antibodies have higher tissue permeability and lower immunogenicity than intact IgG antibodies.
Alpha Lifetech Can Provide
Alpha Lifetech combines proven recombinant antibody expression services with phage display technology to prepare antibodies for customers for a wide range of research, diagnostic and therapeutic applications. The expression of recombinant antibodies is an important part of antibody drug development. By continuously optimizing and improving the expression technology, the quality and yield of antibodies can be improved, which will support the clinical application and industrial development of antibody drugs. According to the different types of host cells, protein expression systems can be categorized into prokaryotic and eukaryotic expression systems. Prokaryotic expression system mainly uses E. coli as the host cell, which has the advantages of easy operation and low cost. We can utilize the prokaryotic expression system to produce large quantities of Fab antibodies in a short period of time. In addition, our laboratory researchers optimize conditions such as induction time, temperature, and reagents to ensure that Fab antibodies are expressed solubly and have antibody activity. We have eukaryotic cell lines, such as mammalian, yeast and insect, that are capable of expressing post-translationally modified Fab antibodies. We will modify and optimize the expression vectors appropriately to improve the expression level and purification efficiency of Fab antibodies.
Protein Expression Systems | Dominance | Expression Cells |
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Mammals expression system | Enables proper protein folding and post-translational modifications | Expi 293F, freestyle 293F, CHO-S, CHO-K1, 293T, etc. |
Escherichia coli expression expression system | Large-scale preparation and easy handling | BL21, Nissle 1917, Rosetta, etc. |
Rodent virus/insect cell protein expression system | Multiple genes can be expressed simultaneously with high intracellular and secreted protein production | Sf9 cells, Sf21 cells, Tn-368 cells, and High Five cells |
Yeast cell expression system | Capable of post-translational modification of proteins, including disulfide bond formation and glycosylation | GS115, X33, etc. |
Fab Antibody Expression Service Process

Fab Antibody Expression Service Workflow
Steps | Specification | Timeline |
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Step1: Fab gene amplification/codon optimization and synthesis | Fab genes that have been screened are amplified by PCR or Fab genes with known sequences are codon optimized and synthesized | 4 weeks |
Step2: Recombinant plasmid construction | Recombinant construction of Fab gene in a suitable expression vector | 2 days |
Step3: Fab antibody expression |
Mammalian Expression Systems
E. coli Expression Expression System
Rodent virus/insect cell protein expression
Yeast Cell Expression Systems
|
2-3 weeks |
Step4: Expression verification | Validation of the expressed antibodies by SDS-PAGE and Western Blot | 1 day |

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hIGH yield
Fab can be expressed by mammalian cells with high protein yield.
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good specificity
Fab antibody with strong activity and good specificity.
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large quantities
With large quantities through E. coli system.
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Multiple study
Functional study of Fab antibody in vitro and in vivo
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Multiple Type
Synthetic and semi-synthetic Fab antibodies can be expressed