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Fab Antibody Expression Service

Alpha Lifetech can prepare Fab antibodies in large quantities by optimizing the culture medium, expression vectors, transfection conditions, etc. for use in drug discovery
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Fab Antibody Expression Service

Introduction of Fab Antibody Expression ServiceFab

Fab, also known as antigen-binding fragment, is a region in the antibody structure that can bind to antigen and consists of a complete light chain (variable and constant regions) and a partial heavy chain structure (variable region and a constant region fragment), with the light chain and the heavy chain connected by a disulfide bond.Fab is small in size, with a molecular weight of 47-48 kDa, and is usually prepared using phage display technology or mammalian cell expression systems. Fab can be used in cancer therapy to target specific tumor antigens or as probes for the detection of specific molecular markers, and is widely used in disease diagnosis and treatment and biological research. Fab antibodies have higher tissue permeability and lower immunogenicity than intact IgG antibodies.
Fab antibody-Alpha Lifetech

Alpha Lifetech Can Provide

Alpha Lifetech combines proven recombinant antibody expression services with phage display technology to prepare antibodies for customers for a wide range of research, diagnostic and therapeutic applications. The expression of recombinant antibodies is an important part of antibody drug development. By continuously optimizing and improving the expression technology, the quality and yield of antibodies can be improved, which will support the clinical application and industrial development of antibody drugs. According to the different types of host cells, protein expression systems can be categorized into prokaryotic and eukaryotic expression systems. Prokaryotic expression system mainly uses E. coli as the host cell, which has the advantages of easy operation and low cost. We can utilize the prokaryotic expression system to produce large quantities of Fab antibodies in a short period of time. In addition, our laboratory researchers optimize conditions such as induction time, temperature, and reagents to ensure that Fab antibodies are expressed solubly and have antibody activity. We have eukaryotic cell lines, such as mammalian, yeast and insect, that are capable of expressing post-translationally modified Fab antibodies. We will modify and optimize the expression vectors appropriately to improve the expression level and purification efficiency of Fab antibodies.

Protein Expression Systems Dominance Expression Cells
Mammals expression system Enables proper protein folding and post-translational modifications Expi 293F, freestyle 293F, CHO-S, CHO-K1, 293T, etc.
Escherichia coli expression expression system Large-scale preparation and easy handling BL21, Nissle 1917, Rosetta, etc.
Rodent virus/insect cell protein expression system Multiple genes can be expressed simultaneously with high intracellular and secreted protein production Sf9 cells, Sf21 cells, Tn-368 cells, and High Five cells
Yeast cell expression system Capable of post-translational modification of proteins, including disulfide bond formation and glycosylation GS115, X33, etc.

Fab Antibody Expression Service Process

Fab antibody production-Alpha Lifetech
Mammalian expression systems usually use transient or stable expression, which can better mimic the expression and modification process of natural proteins, with high efficiency of transcription and translation, and thus enable the production of target proteins with high expression levels. Using mammalian expression systems, such as HEK293 or CHO cell lines, Alpha Lifetech can prepare Fab antibodies in large quantities by optimizing the culture medium, expression vectors, transfection conditions, etc. for use in drug discovery, biologics and other fields. We will perform quality control and performance verification of the expressed Fab antibodies to ensure that the antibodies have antibody affinity and specificity.

Fab Antibody Expression Service Workflow

Steps Specification Timeline
Step1: Fab gene amplification/codon optimization and synthesis Fab genes that have been screened are amplified by PCR or Fab genes with known sequences are codon optimized and synthesized 4 weeks
Step2: Recombinant plasmid construction Recombinant construction of Fab gene in a suitable expression vector 2 days
Step3: Fab antibody expression
Mammalian Expression Systems
E. coli Expression Expression System
Rodent virus/insect cell protein expression
Yeast Cell Expression Systems
2-3 weeks
Step4: Expression verification Validation of the expressed antibodies by SDS-PAGE and Western Blot 1 day

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