Gi24/B7-H5/VISTA
Alpha Lifetech can provide Gi24/B7-H5/VISTA corresponding products, help each customer's research and development.
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Introduction To Gi24/B7-H5/VISTA
Drug targets a biomolecule that can directly bind to drugs and then react. Generally, the target refers to a protein, which is related to the cause of disease. The focus of the target began with focusing on tumors and then began to spread to various fields. Introduction to Gi24/B7-H5/VISTA protein is a common target in medicine.
VISTA, where V-domain Ig suppressor of T cell activation is one of the B7 family members, also called B7-H5, B7H5, C10orf54, Days 1, GI24, PD-1H, SISP 1, VSIR, shares 10 – 18% identity and 23 – 33% similarity at the amino acid level with other B7 family members, and phylogenetically forms group B7 family III with B7x and B7H3. As a new member of the immunoglobulin superfamily, the coding gene located on human chromosome 3q13.13, HHLA 2 is a type I transmembrane protein containing 414 amino acids consisting of an extracellular part, a transmembrane region with a tandem IgV 1-IgC-IgV 2 domain, and a cytoplasmic tail. HHLA 2 is expressed in different species, but not in mice and rats, distinguishing it from other members of the B7 family. HHLA 2 protein is usually expressed on antigen-presenting cells (APC), but it has limited expression in normal human organs.
Function of Target Gi24/B7-H5/VISTA
VISTA maintains the quiescence (quiescence) of T cells and myeloid cells in induced immunosuppressive settings in multiple cancer types and is a promising target for combined tumor immunotherapy. As a co-inhibitory ligand, VISTA can directly inhibit T cell function by binding to undefined receptors on T cells. VISTA can also function as a co-inhibitory receptor for T cells to inhibit T cell function independent of antigen-presenting cells (APC).2. Immune-modulatory function. Overexpression of VISTA on bone marrow-derived dendritic cells reduces T cell proliferation and cytokine production, an effect that can be neutralized by VISTA-specific monoclonal antibodies. VISTA plays an important role in T cell co-suppression, immunomodulation, and anti-tumor immune regulation.
Gene Pathway of Target Gi24/B7-H5/VISTA
VISTA signaling on bone marrow cells suppresses myeloid differentiation primary response 88 (MyD 88) -dependent toll-like receptor (TLR) signaling and cytokine production, thereby inducing a more immunosuppressive phenotype. VISTA activity decreases pro-inflammatory cytokines and increases anti-inflammatory cytokines and mediators. The interaction of V-set and Ig domain 3 (VSIG 3) with VISTA on T cells inhibits the activation and proliferation of T cells. VISTA expressed on cancer cells, MDSCs, or tumor-associated macrophages (tam) can bind to PSGL-1 on T cells at acidic pH, but not at neutral pH. PSGL-1 is linked to anti-PSGL-1 (clone 4RA10) to promote T cell exhaustion by reducing extracellular signal-regulated kinase phosphorylation, AKT and transcriptional signaling and cytokine production, and inhibiting T cell receptor (TCR) signaling and activator 5.
Fig 1: VISTA Gene Pathway. (Reference source: Yuan L, Tatineni J, Mahoney KM, Freeman GJ. VISTA: A Mediator of Quiescence and a Promising Target in Cancer Immunotherapy. Trends Immunol. 2021 Mar;42(3):209-227.)
Alpha Lifetech Can Provide
At present, various drugs targeting Gi24/B7-H5/VISTA are constantly under development, and taking drugs alone or together with other products to treat diseases has become a new method and remarkable achievements have been made. Gi24/B7-H5/VISTA protein has shown important research value and application prospects in tumor biology, and drug research and development. With further research and technological advances, Gi24/B7-H5/VISTA protein products are also essential. Alpha Lifetech can provide Gi24/B7-H5/VISTA corresponding products and help each customer's research and development. Alpha Lifetech focuses on high-precision detection development and validation services to support drug screening and biosimilar discovery. Our detection methods, including ELISA, cell-based functional testing, and flow cytometry, have undergone rigorous sensitivity, specificity, and reproducibility testing. In addition to testing and development, our team also provides data analysis, scheme optimization, and cross-species reactivity testing to ensure reliable results.
| Catalog Number | Product Name | Product Sizes |
|---|---|---|
| ALP64662 | 50ug,100ug,500ug | |
| ALP64593 | ALP64593-Recombinant Mouse Gi24/B7-H5/VISTA Protein, His Tag | 50ug,100ug,500ug |
Reference
[1] Wang J, Wu H, Chen Y, Zhu J, Sun L, Li J, Yao Z, Chen Y, Zhang X, Xia S, Chen W, Shi T. B7-H5 blockade enhances CD8+ T-cell-mediated antitumor immunity in colorectal cancer. Cell Death Discov. 2021 Sep 18;7(1):248.
[2] Bolandi N, Derakhshani A, Hemmat N, Baghbanzadeh A, Asadzadeh Z, Afrashteh Nour M, Brunetti O, Bernardini R, Silvestris N, Baradaran B. The Positive and Negative Immunoregulatory Role of B7 Family: Promising Novel Targets in Gastric Cancer Treatment. Int J Mol Sci. 2021 Oct 3;22(19):10719.
[3] Chen Q, Wang J, Chen W, Zhang Q, Wei T, Zhou Y, Xu X, Bai X, Liang T. B7-H5/CD28H is a co-stimulatory pathway and correlates with improved prognosis in pancreatic ductal adenocarcinoma. Cancer Sci. 2019 Feb;110(2):530-539.
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2018-07-16
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