Sabis na Balagaggewa na Maganin Hana Jiki

Kamfanin Alpha Lifetech Inc.za mu iya bayar da sabis na balaga ta hanyar amfani da fasahar maye gurbi da zaɓi. Yawancin lokaci muna amfani da scFv azaman tsarin rigakafi a cikin hanyoyin sabis na balaga ta hanyar haɗin gwiwa da tsarin phagemid na nuni na monovalent wanda aka yi amfani da shi don rage tasirin sha'awar yayin gwajin ɗaure antigen. Ƙungiyar ƙwararrunmu za ta iya samar da ayyukan balaga ta hanyar haɗin gwiwa don ƙwayoyin rigakafi na yanki ɗaya.

Kamfanin Alpha Lifetech Inc.tana alfahari da bayar da cikakkun ayyuka don biyan buƙatun abokan ciniki daban-daban na duniya. Manufarmu ita ce fahimtar da kuma biyan buƙatun abokan ciniki daban-daban da kuma taimakawa wajen magance duk wata matsala da ke tasowa a ayyukan bincike.

Menene Balagawar Affinity?

Balaga ta Affinity ita ce hanyar da ƙwayoyin rigakafi ke maimaita maye gurbi da zaɓi don haɓaka kusancinsu ga takamaiman ƙwayoyin rigakafi. Yawancinsu suna faruwa ne a tsakiyar ƙwayoyin halittar ƙwayoyin lymphoid na sakandare, ƙwayoyin B suna fuskantar canjin somatic da zaɓi a ƙarƙashin tasirin ƙwayoyin T da ƙwayoyin follicular dendritic.
A lokacin tsarin balaga ta hanyar haɗin kai, ƙwayoyin B masu karɓar immunoglobulin mai ƙarancin alaƙa suna aiki ta hanyar haɗuwa da antigen kuma suna fuskantar yaduwa da bambance-bambance. Ta hanyar balaga ta hanyar haɗin kai ta hanyar haɗin kai ta hanyar haɗin kai, maye gurbin nucleotide bazuwar yana faruwa a cikin yankin da ke canzawa na kwayoyin halittar immunoglobulin, wanda ke haifar da samar da bambance-bambancen antibody daban-daban. Bayan haɓakar haɗin kai ta hanyar haɗin kai, ana yin zaɓin da antigen ke jagoranta akan ƙwayoyin B tare da masu karɓar immunoglobulin masu canzawa. Kwayoyin B waɗanda ke samar da ƙwayoyin rigakafi tare da haɓakar haɗin kai ta hanyar haɗin kai ana fifita riƙe su kuma ana barin su su yaɗu, yayin da ƙwayoyin B waɗanda ke da raguwa ko rashin daidaituwa suna fuskantar apoptosis. Wannan tsari na sauyawa da zaɓi yana haifar da samar da ƙwayoyin B, yana samar da ƙwayoyin rigakafi tare da haɓaka haɗin kai a hankali ga antigen.

Sabis na Balagaggewa na Maganin Hana Jiki

Babban fa'idodin hanyoyin da aka yi amfani da su ta hanyar PCR shine cewa sauye-sauyen suna da alaƙa da ɓangaren da aka ƙara girma; ƙimar kuskuren tana da sauƙin sarrafawa kuma hanyar tana da sauri da sauƙi don saitawa kuma ba ta amfani da sinadarai masu haɗari. An san cewa Taq DNA polymerase yana kwafi DNA tare da ƙarancin aminci, musamman saboda rashin aikin gyarawa na tsawon inci 3 zuwa 5.Alpha Lifetech'sDandalin injiniyan ƙwayoyin cuta yana amfani da hanyar PCR mai saurin canzawa don canza yankuna na CDR galibi yayin gina ƙananan ɗakunan karatu. Kuma sau da yawa muna ƙirƙirar maye gurbi a wurare bazuwar gaba ɗaya a cikin dukkan gutsuttsuran VH da VL, don ƙara bambancin kwayoyin halitta na ƙananan ɗakunan karatu. Bayan gina ɗakin karatu na kwayar halittar ƙwayoyin cuta mai canzawa ta hanyar PCR mai saurin canzawa, zaɓin bambance-bambancen haɗin gwiwa masu girma (ƙarfin ƙwayoyin cuta na scFv na iya kaiwa 10^8 - 10^10 M) ana yin shi ta hanyar jujjuyawa cikin maganin ko kuma akan antigen mai hana motsi tare da yanayin wankewa wanda aka inganta don zaɓin da ya dogara da ƙimar.
  
Masana kimiyya a cikinKamfanin Alpha Lifetech Inc.Yi amfani da nau'ikan mutator na Escherichia coli mutAD5TM a matsayin ɗaya daga cikin dabarun sauye-sauye da dama don gabatar da sauye-sauye bazuwar, ta haka ne za a iya gyara kusanci da bayyanar gutsuttsuran antibody masu sake haɗawa. Ana iya canza yanayin zaɓi don gutsuttsuran antibody tare da matakan samarwa da yawa. Yanayin girma a cikin ƙwayoyin mutator na Escherichia coli za a iya daidaita su don gabatar da maye gurbin maki ɗaya bazuwar a kowace kilobase na DNA, kusan daidai yake da canjin codon ɗaya a kowace guntu na scFv. Bayan zagayowar sauye-sauye da yawa, nuni, da zaɓi, za mu iya inganta daidaiton kusanci daga 10^5 - 10^6 M zuwa 10^8 - 10^10 M.

Inganta Dabarun Balaga na Aure

Ana iya yin bazuwar wasu matsayi na ƙwayoyin rigakafi a wani bambancin da aka ayyana (kamar cikakken bazuwar tare da dukkan amino acid 20 ko kuma bazuwar son zuciya tare da zaɓaɓɓun amino acid a ƙayyadadden kashi) don inganta kusanci. Muna da dabaru guda biyu don inganta kusancin ƙwayoyin rigakafi: na farko, ana gabatar da maye gurbi a wurare masu iyaka a cikin yankuna masu ƙayyadewa (CDRs) ta hanyar mutagenesis da aka jagoranta a wurin; na biyu, ana gabatar da maye gurbi a cikin dukkan yankunan V-coding ta hanyar mutagenesis bazuwar. Ta amfani da waɗannan hanyoyi guda biyu, jerin amino acid da aka kiyaye ko duk yankin tsarin antibody za a maye gurbinsu da wasu amino acid, waɗanda tare da fasahar binciken ɗakin karatu na peptide ɗinmu, na iya ƙara kusancin ƙwayoyin rigakafi masu sha'awa zuwa babban mataki.

Nunin Fage-Abun da ke Hana Kare Jiki

Da zarar an gina ɗakin karatu na mutant na scFv, akwai dabarun tantancewa guda biyu: biopanning da solid-antigen solid. A cikin na farko, ana amfani da wani abu mai rage yawan substrate don ware antibody mai yawan kusanci, kamar yadda za a wanke sauye-sauyen da ba su da alaƙa da juna, wanda zai bar barbashi masu yawan kusanci. Dabaru na biyu yana amfani da antigen mai lakabi a cikin maganin, zaɓi bisa ga daidaitaccen daidaito (Kd), da zaɓi bisa ga haɗin gwiwa, wannan hanyar zaɓi tana ware bambance-bambancen antibody tare da ingantaccen Kd.

Idan kuna da wasu tambayoyi, da fatan za ku iya tuntuɓar mu a kowane lokaci.