Dandalin Ci Gaban Aptamer

Aptamers sune oligonucleotide mai single-stranded (DNA, RNA ko XNA) waɗanda ke da ƙarfin haɗin kai da kuma takamaiman takamaiman abubuwa waɗanda ke ɗaure musamman ga ƙwayoyin da aka yi niyya kamar ƙwayoyin rigakafi, kuma ana amfani da su sosai don haɓaka na'urori masu auna sigina, ganewar asali da kuma maganin cututtuka.

Dandalin Aptamer da Alpha Lifetech ta samar ya ƙunshi nau'i biyu: dandamalin haɗa aptamer, wanda ya ƙunshi sabis ɗin haɗa ɗakin karatu na SELEX aptamer da sabis na haɓaka aptamer (DNA, RNA ko XNA), da kuma dandamalin tantance aptamer wanda ya haɗa da ayyukan tantancewa bisa fasahar SELEX don sunadarai, peptides, ƙwayoyin halitta, ƙananan ƙwayoyin halitta, ions na ƙarfe da sauran ƙwayoyin halitta da aka yi niyya, da kuma ayyukan inganta aptamer da kuma tantancewa.

Dandalin Haɗawa na Aftamer

Sabis ɗin haɗa ɗakin karatu na SELEX aptamer

Sabis na haɗa ɗakin karatu na SELEX aptamer ya ƙunshi gina ɗakin karatu wanda ke ɗauke da adadi mai yawa na jerin oligonucleotide guda ɗaya ta hanyar haɗa sinadarai a cikin vitro bisa ga ƙwayoyin da aka nufa. Gina ɗakin karatu shine wurin fara fasahar SELEX, wanda ke samar da jerin 'yan takara masu yawa don tsarin tantancewa na gaba ta hanyar gina manyan ɗakunan karatu na bazuwar kuma yana ƙara yiwuwar tantance aptamers masu alaƙa da juna.

An raba tsarin tattara laburare zuwa matakai masu zuwa:

Matakai	Cikakkun Bayanan Fasaha
Gano Kwayoyin Manufa	Gano ƙwayoyin da ake buƙata don tantancewa don gano aptamers, waɗanda za su iya zama sunadarai, ƙwayoyin nucleic, ƙananan ƙwayoyin halitta, ions na ƙarfe, da sauransu.
Tsarin Jerin Bazuwar	An tsara tsawon jerin bazuwar, tsarin tushe da sauran sigogi bisa ga halayen ƙwayoyin da aka nufa da buƙatun tantancewa. Yawanci, jerin bazuwar suna tsakanin goma zuwa ɗaruruwan tushe a tsayi.
Tsarin Jerin Kafaffen	An tsara kuma an haɗa gutsuttsuran oligonucleotide masu jerin gwano masu tsayayye (kamar jerin PCR primer) a ƙarshen biyu, waɗanda za a yi amfani da su a cikin tsarin haɓakawa da tantancewa na gaba.

Har yanzu ana buƙatar a ƙara sarrafa ɗakin karatu da aka haɗa don kula da inganci. An ƙaddara yawan ɗakin karatu don tabbatar da amfaninsa a cikin tsarin tantancewa na gaba. An tabbatar da bambancin da daidaito na jerin bazuwar a cikin ɗakin karatu ta hanyar jerawa da sauran hanyoyi don tabbatar da cewa ingancin ɗakin karatu ya cika buƙatun tantancewa.

Ta hanyar matakan da ke sama, ana iya haɗa ɗakin karatu na SELEX aptamer mai inganci da bambancin ra'ayi, wanda zai iya samar da jerin 'yan takara masu yawa don tsarin tantancewa na gaba.

Ayyukan haɓaka Aptamer (DNA, RNA ko XNA)

Aptamers yawanci suna nufin aptamers na nucleic acid. Aptamers na nucleic acid sun haɗa da aptamers na DNA, aptamers na RNA, da kuma aptamers na XNA waɗanda aka gyara su ta hanyar sinadarai. Ana amfani da dabarar SELEX sosai don haɓaka aptamers. Tsarin aiki na asali na ayyukan haɓaka aptamers ya haɗa da gina ɗakin karatu, ɗaure manufa, warewa da tsarkakewa, faɗaɗawa, zagaye da yawa na tantancewa, da kuma gano jerin abubuwa. Tsawon shekaru da yawa, mun mai da hankali kan gina ɗakin karatu da ƙwarewa mai yawa a haɓaka aptamers. Mun himmatu wajen samar wa abokan ciniki ingantattun ayyuka.

Tsarin Fasaha na SELEX

Tsarin SELEX ya ƙunshi zagaye da yawa, kowannensu ya ƙunshi matakai masu zuwa:

Laburare da Haɗa Manufa

An haɗa ɗakin karatu na nucleic acid da takamaiman ƙwayoyin da aka yi niyya (kamar furotin, ƙananan ƙwayoyin halitta, da sauransu), don haka jerin ƙwayoyin halitta a cikin ɗakin karatu suna da damar ɗaurewa da ƙwayoyin da aka yi niyya.

Warewar Kwayoyin Halitta marasa Rufewa

Jerin ƙwayoyin nucleic acid waɗanda ba a haɗa su da kwayar da aka yi niyya ba ana raba su daga cakuda ta hanyoyi na musamman kamar su chromatography na affinity, rabuwar bead na magnetic, da sauransu.

Ƙara Kwayoyin Haɗaka

Ana ƙara yawan jerin nucleic acid da aka ɗaure zuwa wani abu da aka yi niyya, yawanci ana amfani da fasahar polymerase chain reaction (PCR). Don matakin tantancewa na gaba, za a yi amfani da jerin da aka ƙara a matsayin ɗakin karatu na farko.

Hoto na 1: Tsarin tantance SELEX

Dandalin Nuna Aptamer

Sabis na tantancewa na Aptamer

Alpha Lifetech tana ba da nau'ikan ayyukan tantancewa na musamman na aptamer ta amfani da hanyoyin SELEX daban-daban don nau'ikan ƙwayoyin halittar ku:

Nau'ikan Manufa	Cikakkun Bayanan Fasaha
Binciken Protein Aptamer ta SELEX	Babban manufar tantance sinadarin furotin shine a tantance injinan da za su iya ɗaurewa musamman ga ƙwayoyin furotin da aka yi niyya. Waɗannan injinan sun fi sauƙin haɗawa, sun fi karko kuma ba sa fuskantar barazanar muhalli.
Binciken Peptide Aptamer ta SELEX	Peptide aptamers aji ne na gajerun jerin peptide masu cikakken bayani da kusanci, waɗanda za su iya ɗaurewa musamman ga abubuwan da aka nufa kuma su nuna iyawar amfani da su iri-iri a fannin likitanci. Ta hanyar wani takamaiman tsarin tantancewa, ana tantance peptide aptamers waɗanda za su iya ɗaurewa musamman ga ƙwayoyin da aka nufa daga adadi mai yawa na ɗakunan karatu na jerin peptide.
Binciken Aptamer na musamman ga ƙwayoyin halitta (SELEX)	Ana shirya ƙwayoyin da aka nufa ko takamaiman ƙwayoyin da ke saman tantanin halitta a matsayin abubuwan da aka nufa. Abubuwan da aka nufa na iya zama ƙwayoyin halitta gaba ɗaya, masu karɓa a jikin membrane na tantanin halitta, sunadarai, ko wasu ƙananan ƙwayoyin halitta.
Ƙaramin Allon Ƙwayoyin Halitta ta hanyar Kama SELEX	Capture SELEX wata dabara ce ta tantancewa ta in vitro don tantance ƙananan aptamers na ƙwayoyin halitta, wanda shine nau'in SELEX. Capture SELEX ya dace musamman don tantance ƙananan ƙwayoyin halitta, waɗanda galibi suna da ƙarancin ƙungiyoyi masu aiki kuma suna da wahalar hana motsi kai tsaye akan tallafin matakan ƙarfi.
Ayyukan SELEX na tushen dabbobi kai tsaye	Sabis na tantance dabbobi masu rai wata dabara ce ta gwaji da ake amfani da ita sosai a fannonin kimiyyar halittu, magani da fasahar halittu, wanda ke amfani da dabbobi masu rai a matsayin samfuran gwaji don tantancewa da kimanta takamaiman ƙwayoyin halitta, likitanci, hanyoyin warkewa ko na halittu. An tsara ayyukan ne don kwaikwayon yanayin ilimin halittar jiki a jikin ɗan adam don yin hasashen da kimanta inganci da amincin sakamakon gwaji a jikin ɗan adam daidai.

Sabis na inganta Aptamer

Rashin isasshen ruwa, yawan asarar da ake samu yayin samarwa, da kuma fitar da aptamers cikin sauri yana takaita amfani da su. A halin yanzu, an binciko hanyoyi daban-daban na ingantawa don inganta aikin aptamers.

Muna da hanyoyi daban-daban don inganta aptamer wanda ya ƙunshi yankewa, gyare-gyare, haɗawa zuwa rukunin da ya dace (thiol, carboxy, amine, fluorophore, da sauransu).

Sabis na nazarin halayen Aptamer

Sabis na nazarin halayen Aptamer yana nufin sabis na ƙwararru na ƙudurin tsarin kimanta aiki da tabbatar da aiki na aptamer da aka samu don tabbatar da cewa aptamer ya cika takamaiman iyawa ta ɗaurewa, kwanciyar hankali da takamaiman buƙatu. Ya ƙunshi galibin binciken kusanci da takamaiman abu, kimanta kwanciyar hankali da tabbatar da aikin halittu.