Dandalin Fasaha na Hybridoma
Alpha Lifetech tana ba wa abokan ciniki kayayyaki da ayyuka masu inganci na rigakafi mai inganci da kuma na rigakafi masu sake haɗawa.
Nemi Farashin Kuɗi01
Dandalin Fasaha na Hybridoma
Alpha Lifetech tana da ƙwarewa mai yawa a fannin haɓaka ƙwayoyin rigakafi. Tsawon shekaru, Alpha Lifetech ta kafa wani dandamali na haɓaka ƙwayoyin rigakafi (gami da haɓaka ƙwayoyin rigakafi na monoclonal, haɓaka ƙwayoyin rigakafi na polyclonal, haɓaka ƙwayoyin rigakafi na monoclonal da aka sake haɗawa, da sauran ayyukan shirye-shiryen ƙwayoyin rigakafi da haɓaka ƙwayoyin rigakafi).
Alpha Lifetech tana ba wa abokan ciniki kayayyaki da ayyuka masu inganci na rigakafi da kuma ayyukan rigakafi masu sake haɗawa, kuma ƙwayoyin rigakafi da aka shirya suna da ƙwarewa mai ƙarfi da kwanciyar hankali mai kyau. Ana iya amfani da kayan aiki da kayan aiki da yawa na tsarkake ƙwayoyin rigakafi don samar da ayyukan tsarkake ƙwayoyin rigakafi daga tushe daban-daban kamar zomo, tumaki, kaza, ƙwayoyin rigakafi na monoclonal na linzamin kwamfuta, da kuma ayyukan tsarkake furotin A/G da ayyukan rabuwa da ƙwayoyin cuta.
Dangane da dabarun shirye-shiryen rigakafi daban-daban kamar fasahar ƙwayoyin hybridoma, Alpha Lifetech na iya samar da ayyuka na musamman don ƙwayoyin rigakafi na monoclonal da polyclonal na nau'ikan daban-daban. Bugu da ƙari, ana iya samar da ayyuka iri-iri daga jerin abubuwan da ake buƙata, shirya ƙwayoyin rigakafi, tsarkake ƙwayoyin rigakafi zuwa tabbatar da ƙwayoyin rigakafi. Za mu zaɓi hanyar tsarkakewa bisa ga takamaiman manufar abokin ciniki. Dangane da tsarin dandamali mai cikakken tsari kamar dandamalin haɓaka ƙwayoyin rigakafi da dandamalin furotin, Alpha Lifetech yana rufe ayyukan samar da ƙwayoyin rigakafi na sama da ƙasa, samar da tsarkake ƙwayoyin rigakafi, jerin abubuwan da ake buƙata na ƙwayoyin rigakafi, tabbatar da ƙwayoyin rigakafi da sauran ayyukan fasaha.
Fasaha ta Hybridoma
Ana iya amfani da fasahar Hybridoma don shirya ƙwayoyin rigakafi na monoclonal. Ta hanyar haɗa ƙwayoyin spleen daga beraye da aka yi musu rigakafi tare da ƙwayoyin myeloma daga mutane ko beraye, ana samar da ƙwayoyin hybridoma kuma suna fitar da takamaiman ƙwayoyin rigakafi. Ana iya amfani da ƙwayoyin rigakafi na monoclonal da aka samo daga linzamin kwamfuta a cikin binciken kimiyya kamar gwajin immunosorbent mai alaƙa da enzyme, radioimmunoassay, immunohistochemistry, kwararar cytometry, tsarkake furotin, da sauransu. Samfuran ƙwayoyin rigakafi na monoclonal sun haɗa da ascites, hybridoma supernatant, da sauransu. Samfurin da aka samu ta hanyar allurar ƙwayoyin hybridoma zuwa cikin ramin ciki na dabbobi da kuma amfani da yanayin ilimin halittar jiki na ramin ciki na dabba don samar da ƙwayoyin rigakafi ana kiransa samfurin ascites. Kwayoyin Hybridoma da aka allura a cikin ramin ciki na dabbobi na iya yaduwa da kuma fitar da ƙwayoyin rigakafi na monoclonal, waɗanda za su iya taruwa a cikin ascites. Saboda yiwuwar ƙazanta (sunadaran dabbobi, hormones, da sauransu) a cikin waɗannan ƙwayoyin rigakafi, ana buƙatar tsarkakewa. Dangane da aikin fitar da ƙwayoyin rigakafi na B, ana fitar da ƙwayoyin rigakafi na monoclonal zuwa cikin tsakiyar al'ada lokacin da ake haɓaka ƙwayoyin hybridoma. Ruwan da ke ɗauke da ƙwayoyin rigakafi na monoclonal a cikin tsakiyar al'ada ana kiransa hybridoma supernatant. Tsarkakakken ƙwayoyin rigakafi na monoclonal a cikin ruwan da ke cikinsa yana da yawa, amma ana buƙatar ƙarin tsarkakewa don shiri mai girma kamar yadda ake samu ta hanyar al'adar in vitro.
Tsaftace garkuwar jiki na iya ware ƙwayoyin rigakafi masu tsarki daga cikin hadaddun ƙwayoyin cuta. A halin yanzu, tsarkake garkuwar jiki da rabuwa da tsarkake ƙwayoyin cuta su ne hanyoyin tsarkake ƙwayoyin cuta da aka fi amfani da su, waɗanda suka haɗa da tsarkake furotin A/G, chromatography na musayar ion, tace gel, ruwan sama da kuma chromatography na hulɗar hydrophobic. Daga cikinsu, sabis na tsarkake furotin A/G yana da shahara sosai a aikace-aikacen tsarkake ƙwayoyin cuta daban-daban. Tsarin Alpha Lifetech na shirya ƙwayoyin rigakafi na monoclonal bisa fasahar hybridoma an nuna shi a Hoto na 1.
Hoto na 1. Tsarin tsari don shirya ƙwayoyin rigakafi na monoclonal bisa fasahar hybridoma.
Haɗin Magungunan Antibody da Fasahar Hybridoma
Maganin hana garkuwar jiki (ADC) wani sabon magani ne na halitta wanda ke haɗa ƙwayoyin rigakafi tare da magungunan cytotoxic. Maganin hana garkuwar jiki yana haɗa takamaiman ikon gane ƙwayoyin rigakafi tare da tasirin kashe ƙwayoyin cuta masu guba, kuma yana isar da magungunan cytotoxic daidai cikin ƙwayoyin ƙari ta hanyar ƙwayoyin rigakafi, don haka ana samun ingantaccen magani. Rabon ƙwayoyin rigakafi a cikin magungunan ADC galibi ana shirya shi ta hanyar fasahar hybridoma ko wasu dabarun samar da ƙwayoyin rigakafi, kamar fasahar injiniyan kwayoyin halitta. Fasahar Hybridoma tana ba da kayan aikin rigakafi masu inganci don magungunan ADC. Magungunan ADC da ƙwayoyin rigakafi na monoclonal waɗanda aka shirya ta hanyar fasahar hybridoma suna taka muhimmiyar rawa a fannoni kamar maganin ciwon daji. Ana iya amfani da su su kaɗai ko a hade don haɓaka ingancin magani.
Kwayoyin rigakafi na monoclonal da fasahar hybridoma ta shirya suna da takamaiman takamaiman bayanai, suna iya ɗaurewa daidai da gane takamaiman kwayoyin rigakafi, kuma ana iya samar da su akan babban sikelin. Ta hanyar fasahar jerin kwayoyin halitta na hybridoma, ana iya cire kwayoyin halittar rigakafi a cikin ƙwayoyin hybridoma kuma a jera su don samun jerin da suka dace, wanda ke da amfani ga bayyanar kwayoyin halitta na gaba. Kwayoyin rigakafi na monoclonal da aka shirya ta hanyar fasahar hybridoma za a iya amfani da su don gano takamaiman alamun cututtuka, kamar alamun ƙari, antigens na ƙwayoyin cuta, da sauransu, suna ba da tallafi mai ƙarfi don gano cututtuka da wuri. Bugu da ƙari, ana iya amfani da shi a cikin gwaje-gwaje kamar immunoblotting da immunohistochemistry don nazarin bayyanar furotin, wurin zama, tsari, da aiki.
fasahar hybridoma Production Tsarin aiki na rigakafi
| Matakai | Abubuwan Sabis | Tsarin lokaci |
|---|---|---|
| Mataki na 1: Rigakafin dabbobi | (1) Rigakafin dabbobi sau 4, allurar rigakafin ƙara girma kashi 1, jimillar allurai 5 da aka yi wa allurar rigakafi. (2) An tattara maganin rigakafi mara kyau kafin a yi allurar riga-kafi, kuma an yi wa ELISA allura ta huɗu don gano titer na jini. (3) Idan adadin maganin rigakafi na jini na kashi na huɗu ya cika buƙatun, za a ba da ƙarin allurar rigakafi kwana 7 kafin a ɗauki jini. Idan bai cika buƙatun ba, za a ci gaba da yin allurar rigakafi ta yau da kullun. (4) Ƙarfin da ya cancanta, ƙwayoyin Spleen suna keɓewa don haɗa ƙwayoyin halitta. | Makonni 10-12 |
| Mataki na 2: Haɗawa da Nunawa | (1) Haɗawa: Bayan allurar rigakafi, an tattara ƙwayoyin halittar saifa na beraye aka haɗa su da ƙwayoyin myeloma. (2) Yaɗuwa da tantancewa: An yi wa ƙwayoyin hybridoma da aka samu yaɗuwa da kuma tantance ELISA don samun ƙwayoyin clone masu inganci sosai. | Makonni 6 |
| Mataki na 3: Subclone | Yin amfani da ƙananan ƙwayoyin halitta masu inganci guda 15-25 ta hanyar amfani da hanyar rage yawan ƙwayoyin halitta, sannan a ƙarshe a tantance ƙwayoyin halitta masu inganci guda 10-15 | Makonni 3-4 |
| Mataki na 4: Samar da Ascites da Tsarkakewa daga Kwayoyin cuta | (1) Samar da Ascites: Ana amfani da nau'ikan hybridoma 10-15 don samar da ascites a cikin beraye (idan an sami adadi mai yawa na nau'ikan hybridoma, za a aika su ta hanyar ƙwayoyin halitta) (2) Gano Ascites: Gano Ascites ELISA, ƙarfin aiki (protein/virus>10 ^ 5, ƙaramin ƙwayar cuta/peptide>10 ^ 4) don tsarkakewa daga ƙasa (3) Tsarkakewar Sunadaran A/G: Tsarkakewar Ascites | Makonni 3-4 |
| Mataki na 5: Isarwa | (1) Samar da nau'ikan ƙwayoyin rigakafi guda 3-5 na monoclonal hybridoma. (2) Kowace ƙwayar hybridoma tana samar da 1mg na maganin rigakafi mai tsabta (tsarkakewar ascites). (3) Rahoton Tsarin Gwaji | Mako 1 |
Nau'o'i Da Yawa
Ana iya samar da kariya ga dabbobi daban-daban, ciki har da amma ba'a iyakance ga beraye, zomaye, tumaki, da sauransu ba.
Manufa Da Yawa
Dangane da fasahar hybridoma, ana samun ayyukan gano ƙwayoyin cuta da yawa da aka yi niyya: sunadarai, peptides, ƙananan ƙwayoyin cuta, ƙwayoyin cuta, sunadaran membrane, mRNA, da sauransu.
Bayanan Bibiyar Abubuwan Da Suka Shafi Rayuwa
Ka'idojin kula da ingancin QC (ƙarfin garkuwar jiki, kula da ingancin PBMC, kula da ingancin ɗakin karatu, da kuma kula da ingancin tantancewa), rahotannin gwaji na Ingilishi, bayanan gwaji na asali
Shirin Keɓancewa
Keɓancewa na shirin mutum ɗaya-ɗaya don biyan buƙatun aikin bincike na abokan ciniki daban-daban
Sabis na Haɓaka Magungunan Hana Cututtukan Monoclonal
Za mu iya samar da ayyuka masu inganci, tsafta, da kuma takamaiman ayyuka na haɓaka ƙwayoyin cuta na monoclonal, gami da samar da ƙwayoyin rigakafi na monoclonal na linzamin kwamfuta da ƙwayoyin rigakafi na zomo na monoclonal
Dandalin Fasaha na Hybridoma
Ya haɗa da shirin rigakafi, ayyukan shirya antibody, tsarkake antibody, jerin abubuwan da antibody ke samarwa, tabbatar da antibody, da sauransu
Dandalin Rarraba Ƙwayar B Guda ɗaya
Alpha Lifetech tana da fa'idodi a lokacin tantancewa da kuma samun ingantattun ƙwayoyin rigakafi. Tana iya samar da ƙira, haɗawa, da gyarawa, garkuwar dabbobi, tantance haɓaka ƙwayoyin B guda ɗaya, da kuma jerin ƙwayoyin halitta guda ɗaya.
Dandalin Haɓaka Maganin Kariya Daga Fage Display Antibody
Alpha Lifetech na iya samar da ayyukan fasaha na haɓaka ƙwayoyin cuta na phage display antibody daga shirye-shiryen ƙwayoyin cuta, tsarkake ƙwayoyin cuta, jerin ƙwayoyin cuta, da sauransu.
Albarkatun Fasaha Masu Alaƙa
Gabatarwa ga Fasahar HybridomaBIDIYO
Idan kuna da wasu tambayoyi, da fatan za ku iya tuntuɓar mu a kowane lokaci.
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2018-07-16 
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