Dandali na Haɓaka Haɓaka Mataki

Alfa Lifetach, tare da babban aikin aikinta a cikin binciken da aka gano, na iya samar da sabis na musamman da kuma ayyukan rigakafi na kwastomomi da ayyukan ɗakunan karatu. Alpha Lifetech na iya ba abokan ciniki ingantaccen ingantaccen rigakafin rigakafi da samfuran rigakafin sake haɗawa, kazalika da sabis masu dacewa, don shirya ingantaccen, takamaiman takamaiman, da tsayayyen ƙwayoyin cuta. Ta hanyar amfani da ingantattun dandamali na antibody da injiniyan antibody, muna ba da sabis wanda ke rufe sama da ƙasa na samar da rigakafin, gami da sabis na fasaha kamar shirye-shiryen antibody, tsarkakewa, jerin antibody, da inganci.

Alpha Lifetech yana da babban dandamalin gano ƙwayoyin cuta wanda ke ba abokan ciniki kewayon sabis waɗanda suka haɗa da rigakafi na dabba, gini na phage antibody ginin ɗakin karatu da dubawa, jerin antibody, maganganun antibody, tsarkakewar antibody, ingantaccen antibody, da lakabin antibody dangane da fasahar hybridoma, fasahar tantanin halitta B guda ɗaya, fasahar nunin phage, da ƙari. Alpha Lifetech yana da daidaitaccen layin samarwa, kuma ban da sabis na shirye-shiryen antibody, ana iya ba da sabis na tallafi kamar antibody humanization, antibody affinity maturation, ADC miyagun ƙwayoyi da haɓakawa, da kuma CAR-T ƙira ta gaba. A lokaci guda, Alpha Lifetech ya gina ɗakin karatu na antibody wanda ya dogara da dandamali na fasahar nunin M13, T4, da T7 phage, tare da damar ajiya har zuwa 10^8-10^9. Ingantacciyar ƙima, ƙimar shigarwa, da bambancin ɗakin karatu na iya kaiwa sama da 90%.

Fasaha nunin Fage

An fara shirya samar da ƙwayoyin rigakafi na Monoclonal ta amfani da fasahar antibody hybridoma monoclonal don samar da maganin rigakafi na linzamin kwamfuta na hybridoma monoclonal. Musamman, ta hanyar haɗa ƙwayoyin saɓo daga ƙwayoyin rigakafi tare da ƙwayoyin myeloma daga mutane ko mice, ana samun sel hybridoma, waɗanda ke ɓoye takamaiman ƙwayoyin rigakafin ƙwayoyin cuta na monoclonal hybridoma. Ta hanyar gyare-gyaren ɗan adam na ƙwayoyin rigakafi na monoclonal na linzamin kwamfuta da gyare-gyaren injiniyan kwayoyin halitta na ƙwayoyin rigakafi don ba su yanki na immunoglobulin na ɗan adam akai-akai, ana iya rage rigakafirsu. Monoclonal antibody injiniyoyi ana amfani da ko'ina a biomedical da na asibiti aikace-aikace.

Alpha Lifetech na iya ba da sabis na injiniyan rigakafin mutum tasha ɗaya daga ƙirar ƙa'idar bincike ta ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙwayoyin cuta (ADCs) ganowa da ingancin dabba. Magungunan magungunan rigakafi (ADCs) na iya isar da magungunan chemotherapy zuwa ƙwayoyin kansa. Bayan daure ga takamaiman maƙasudai da aka bayyana akan ƙwayoyin kansa, ADC tana sakin magungunan cytotoxic a cikin ƙwayoyin kansa. Alpha Lifetech kuma na iya ba abokan ciniki cikakkiyar sabis na maturation na antibody. Tare da haɓaka haɓakar maye gurbi da fasaha mai nunin phage mai girma, ana fara tantance ƙwayoyin rigakafi masu alaƙa da alaƙa, sannan kuma ana gabatar da maye gurbin amino acid don samar da bambance-bambance daban-daban. Bayan haka, an tantance ƙwayoyin rigakafi masu girma kuma an duba su ta amfani da dabarun tantancewa. Bayan zagaye da yawa na ingantawa da bincike na tsari, a ƙarshe an sami ƙwayoyin rigakafi masu alaƙa da ƙaƙƙarfan ƙayyadaddun ƙayyadaddun bayanai.

Alpha Lifetech na iya gina nau'ikan dakunan karatu na nunin antibody, gami da dakunan karatu na rigakafi, dakunan karatu na asali, dakunan karatu na roba, da dakunan karatu na roba. Dangane da babban ƙarfin ɗakunan karatu na antibody, ana iya samun takamaiman ƙwayoyin rigakafi. Ana iya samar da ƙwayoyin phagemid da yawa kamar pMECS, pComb3X, da pCANTAB 5E, da kuma nau'i irin su TG1 E. coli, XL1 Blue, da ER2738. Baya ga ƙarfin ɗakin karatu na har zuwa 10^9, ƙimar shigar guntuwar ɗakin ɗakin karatu shima yana da girma, yana samar da isassun yanayi don tantance ƙwayoyin rigakafi waɗanda ke gamsar da abokan ciniki. Taswirar kwararar sabis na shirye-shiryen rigakafin jiki na Alpha Lifetech dangane da fasahar nunin phage ana nuna shi a cikin Hoto 1.

Hoto na 1 Tsarin tsari don shirya ƙwayoyin rigakafi na monoclonal bisa fasahar nunin phage.

Hannun Mataki na Ƙarfafa Ayyukan Samar da Jiki

Matakai	Abubuwan Sabis	Tsarin lokaci
Mataki 1: rigakafin dabbobi	(1) rigakafi na dabba sau 4, inganta rigakafi kashi 1, jimlar allurai 5 an yi rigakafi. (2) Magani mara kyau kafin a tattara rigakafi, kuma an yi ELISA akan kashi na huɗu don gano titer na jini. (3) Idan ma'aunin maganin ƙwayar cuta na kashi na huɗu ya cika buƙatun, ƙarin kashi ɗaya na rigakafi za a ba shi kwanaki 7 kafin tarin jini. Idan bai cika buƙatun ba, za a ci gaba da yin rigakafi na yau da kullun. (4) Ingantacciyar ƙarfi, tarin jini da rabuwar monocytes	makonni 10
Mataki 2: cDNA Shiri	(1) PBMC Jimlar Cirar RNA (Kitin Haɗin RNA) (2) Babban amincin RT-PCR shirye-shiryen cDNA (kit ɗin rubutun juyi)	kwana 1
Mataki 3: Gina Laburaren Antibody	(1) Yin amfani da cDNA azaman samfuri, kwayoyin halitta sun haɓaka ta zagaye biyu na PCR. (2) Tsarin gini da canji: gene splicing phagemid vector, electroporation chanji na TG1 rundunar kwayoyin cuta, gina antibody library. (3) Identification: Zabi 24 clones da gangan, ƙimar ƙimar ƙimar PCR + ƙimar shigarwa. (4) Taimakon shirye-shiryen phage: M13 phage amplification+ tsarkakewa. (5) Fage nunin ɗakin karatu	3-4 makonni
Mataki 4: Antibody Library Screening (zagaye 3)	(1) Tsohuwar 3-zagaye nunawa (tsananin nunawa): gwajin matsa lamba don cire ƙwayoyin rigakafi marasa takamaiman zuwa iyakar iyaka. (2) Single clone amplification bacteriophage zaba + IPTG jawo magana + ELISA gano tabbatacce clones. (3) An zaɓi duk ƙaƙƙarfan clones don jerin abubuwan halitta.	4-5 makonni

Amfanin Fasahar Nuni na phage

Alpha Lifetech yana da wadataccen gogewa a fagen ci gaban antibody. A cikin shekaru da yawa, Alpha Lifetech ya kafa ingantaccen dandamali na ci gaban antibody.

Ayyukan Tallafawa

Za mu iya ba da sabis na ginin ɗakin karatu na rigakafi na dabba daban-daban da sabis na tantance laburaren jikin mutum bisa ga bukatun abokin ciniki

Manufa da yawa

Akwai hidimomin ganowar rigakafin da yawa da yawa: sunadaran, peptides, ƙananan ƙwayoyin cuta, ƙwayoyin cuta, sunadaran membrane, mRNA, da sauransu.

Matsaloli da yawa

Sabis na ginin ɗakin karatu na keɓaɓɓen, za mu iya samar da nau'ikan ƙwayoyin cuta daban-daban ciki har da PMECS, pComb3X, da pCANTAB 5E, da gyara su bisa ga bukatun abokin ciniki.

Balagagge Platform

Ƙarfin ajiya zai iya kaiwa 10 ^ 8-10 ^ 9, ƙimar shigarwa duk sun fi 90%, kuma alaƙar ƙwayoyin rigakafi da aka samu ta hanyar nunawa gabaɗaya a matakin nM pM

SERVICE masu alaƙa

Dabarun Ci gaban Kayayyakin Kaya da yawa

Monoclonal Antibody Development Service

Dandalin Fasahar Hybridoma

Dandali Na Rarraba Cell Single B

Dandali na Haɓaka Haɓaka Mataki

Ci gaban antibody monoclonal-Alpha Lifetech

Monoclonal Antibody Development Service

Za mu iya samar da ingantacciyar inganci, tsafta, da takamaiman sabis na ci gaban antibody na monoclonal, gami da samar da ƙwayoyin rigakafin ƙwayoyin cuta na linzamin kwamfuta da ƙwayoyin rigakafin zomo monoclonal.

Dandalin Fasahar Hybridoma

Ciki har da shirin rigakafi, sabis na shirye-shiryen rigakafin jiki, tsarkakewar antibody, jerin abubuwan da ake buƙata na antibody, ingantaccen maganin rigakafi, da sauransu.

Dandali Na Rarraba Cell Single B

Alpha Lifetech yana da fa'idodi a lokacin dubawa da samun ingantattun ƙwayoyin rigakafi. Yana iya samar da ƙirar antigen, haɗawa, da gyare-gyare, rigakafi na dabba, gwajin haɓakar ƙwayoyin ƙwayoyin B guda ɗaya, jerin tantanin halitta guda ɗaya.