Dandalin Haɓaka Maganin Kariya Daga Fage Display Antibody
Dangane da cikakken tsarin dandamali na tsarin tsarin rigakafi, Alpha Lifetech na iya samar da ayyukan fasaha daga shirye-shiryen rigakafi, tsarkake garkuwar jiki, jerin garkuwar jiki, da sauransu.
TUntuɓe Mu01
Dandalin Haɓaka Maganin Kariya Daga Fage Display Antibody
Alpha Lifetech, tare da ƙwarewar aikinta mai zurfi a binciken ƙwayoyin cuta, na iya samar da ayyukan ƙwayoyin cuta na monoclonal da polyclonal na musamman ga nau'ikan halittu daban-daban, da kuma gina ɗakunan karatu da ayyukan tantance ƙwayoyin cuta. Alpha Lifetech na iya samar wa abokan ciniki ingantattun samfuran ƙwayoyin cuta da ƙwayoyin cuta masu sake haɗawa, da kuma ayyuka masu dacewa, don shirya ƙwayoyin cuta masu inganci, takamaiman, da kuma karko. Ta hanyar amfani da cikakkun dandamalin ƙwayoyin cuta da injiniyan ƙwayoyin cuta, muna samar da ayyuka da suka shafi samar da ƙwayoyin cuta na sama da ƙasa, gami da ayyukan fasaha kamar shirya ƙwayoyin cuta, tsarkakewa, jerin ƙwayoyin cuta, da tabbatarwa.
Alpha Lifetech tana da wani dandamali na gano ƙwayoyin cuta masu girma wanda ke ba wa abokan ciniki ayyuka iri-iri, ciki har da garkuwar jiki ga dabbobi, gina ɗakin karatu na ƙwayoyin cuta masu nuna ƙwayoyin cuta na phage da kuma tantance su, jerin ƙwayoyin cuta masu nuna ƙwayoyin cuta, bayyanar ƙwayoyin cuta masu nuna ƙwayoyin cuta, tsarkake ƙwayoyin cuta masu nuna ƙwayoyin cuta, tabbatar da ƙwayoyin cuta masu nuna ƙwayoyin cuta, da kuma laƙabin ƙwayoyin cuta masu nuna ƙwayoyin cuta bisa fasahar hybridoma, fasahar ƙwayoyin cuta guda ɗaya ta B, fasahar nuna ƙwayoyin cuta, da ƙari. Alpha Lifetech tana da layin samarwa na yau da kullun, kuma baya ga ayyukan shirya ƙwayoyin cuta, ana iya samar da ayyuka masu tallafi kamar su haɓaka ƙwayoyin cuta masu nuna ƙwayoyin cuta, balaga da haɓakar ƙwayoyin cuta masu nuna ƙwayoyin cuta, ƙirar magunguna da haɓaka magungunan ADC, da kuma ƙirar jerin abubuwan da ke biyo baya na CAR-T. A lokaci guda, Alpha Lifetech ta gina ɗakin karatu na ƙwayoyin cuta masu nuna ƙwayoyin cuta bisa dandamalin fasahar nunin ƙwayoyin cuta na M13, T4, da T7, tare da ƙarfin ajiya har zuwa 10^8-10^9. Matsakaicin ƙimar da aka samu, ƙimar shigarwa, da bambancin ɗakin karatu duk suna iya kaiwa sama da kashi 90%.
Fasahar Nunin Fage
An fara shirya samar da ƙwayoyin rigakafi na monoclonal ta amfani da fasahar hybridoma monoclonal antibody don samar da ƙwayoyin rigakafi na monoclonal na linzamin kwamfuta. Musamman ma, ta hanyar haɗa ƙwayoyin spleen daga beraye da aka yi musu allurar rigakafi tare da ƙwayoyin myeloma daga mutane ko beraye, ana samar da ƙwayoyin halitta na hybridoma, waɗanda ke fitar da takamaiman ƙwayoyin rigakafi na monoclonal antibody na hybridoma. Ta hanyar sauye-sauyen ƙwayoyin rigakafi na monoclonal na linzamin kwamfuta da gyaran ƙwayoyin rigakafi na kwayoyin halitta don ba su yankin immunoglobulin na ɗan adam akai-akai, za a iya rage ƙarfin garkuwar jikinsu. Ana amfani da injiniyan ƙwayoyin rigakafi na monoclonal sosai a aikace-aikacen likitanci da na asibiti.
Alpha Lifetech na iya samar da ayyukan injiniyan rigakafi na tsayawa ɗaya daga ƙirar ka'idojin bincike na asibiti zuwa gano ƙwayoyin rigakafi (ADCs) da kuma tabbatar da ingancin dabbobi. Ƙungiyoyin magungunan rigakafi (ADCs) na iya isar da magungunan chemotherapy ga ƙwayoyin cutar kansa. Bayan ɗaurewa ga takamaiman abubuwan da aka fi mayar da hankali a kan ƙwayoyin cutar kansa, ADC tana sakin magungunan cytotoxic zuwa cikin ƙwayoyin cutar kansa. Alpha Lifetech kuma na iya samar wa abokan ciniki cikakkun ayyukan balaga na antibody. Tare da ingantaccen sauye-sauye da fasahar tantance phage mai ƙarfi, ana fara tantance ƙwayoyin rigakafi masu takamaiman sauye-sauye, sannan a gabatar da sauye-sauyen amino acid don samar da bambance-bambancen daban-daban. Daga baya, an tantance kuma an tantance ƙwayoyin rigakafi masu ƙarfi ta amfani da dabarun tantancewa. Bayan zagaye da yawa na ingantawa da nazarin tsari, an sami ƙwayoyin rigakafi masu ƙarfi da ƙarfi.
Alpha Lifetech na iya gina nau'ikan ɗakunan karatu daban-daban na nunin ƙwayoyin cuta na antibody phage, gami da ɗakunan karatu na rigakafi, ɗakunan karatu na asali, ɗakunan karatu na semi-synthetic, da ɗakunan karatu na roba. Dangane da ƙarfin ɗakunan karatu na antibody, ana iya samun ƙwayoyin rigakafi na musamman. Ana iya samar da ƙwayoyin cuta na phagemid da yawa kamar pMECS, pComb3X, da pCANTAB 5E, da kuma nau'ikan ƙwayoyin cuta kamar TG1 E. coli, XL1 Blue, da ER2738. Baya ga ƙarfin ɗakin karatu har zuwa 10^9, ƙimar shigar da guntu na ɗakin karatu shi ma yana da yawa, yana ƙirƙirar isassun yanayi don tantance ƙwayoyin cuta waɗanda ke gamsar da abokan ciniki. An nuna jadawalin aikin shirye-shiryen ƙwayoyin cuta na Alpha Lifetech bisa fasahar nunin ƙwayoyin cuta a cikin Hoto na 1.
Hoto na 1. Tsarin tsari don shirya ƙwayoyin rigakafi na monoclonal bisa fasahar nunin phage.
Tsarin Aikin Samar da Maganin Kariya daga Kwayoyin cuta na Phage
| Matakai | Abubuwan Sabis | Tsarin lokaci |
|---|---|---|
| Mataki na 1: Rigakafin dabbobi | (1) Rigakafin dabbobi sau 4, allurar rigakafin ƙara girma kashi 1, jimillar allurai 5 da aka yi wa allurar rigakafi. (2) An tattara maganin rigakafi mara kyau kafin a yi allurar riga-kafi, kuma an yi wa ELISA allura ta huɗu don gano titer na jini. (3) Idan adadin maganin rigakafi na jini na kashi na huɗu ya cika buƙatun, za a ba da ƙarin allurar rigakafi kwana 7 kafin a ɗauki jini. Idan bai cika buƙatun ba, za a ci gaba da yin allurar rigakafi ta yau da kullun. (4) Ingancin ƙarfi, tattara jini da kuma raba ƙwayoyin halitta masu rai (monocytes) | Makonni 10 |
| Mataki na 2: Shiri na cDNA | (1) Jimlar Cirewar RNA ta PBMC (Kit ɗin Cirewar RNA) (2) Tsarin RT-PCR mai inganci na cDNA (kayan kwafi na baya) | kwana 1 |
| Mataki na 3: Gina Laburaren Magungunan Kariya | (1) Ta amfani da cDNA a matsayin samfuri, an ƙara yawan kwayoyin halitta ta hanyar zagaye biyu na PCR. (2) Gina da kuma sauyi a cikin tsarin kwayoyin halitta: phagemid vector da ke haɗa kwayoyin halitta, canjin electroporation na ƙwayoyin cuta masu karɓar TG1, gina ɗakin karatu na antibody. (3) Ganewa: Zaɓi kwafi 24 ba zato ba tsammani, ƙimar tantancewar PCR mai kyau + ƙimar sakawa. (4) Shirye-shiryen phage da aka taimaka: Ƙarfafa phage na M13+ tsarkakewa. (5) Ceto ɗakin karatu na nunin faifai | Makonni 3-4 |
| Mataki na 4: Allon Laburare na Antibody (zagaye 3) | (1) Binciken zagaye 3 na asali (dubawa mai ƙarfi): gwajin matsin lamba don cire ƙwayoyin rigakafi marasa takamaiman gwargwadon iyawa. (2) An zaɓi bacteriophage mai ƙara yawan ƙwayoyin cuta guda ɗaya + bayyanar da IPTG ta haifar + gano ELISA na ƙwayoyin cuta masu kyau. (3) An zaɓi dukkan ƙwayoyin halitta masu kyau don tsara kwayoyin halitta. | Makonni 4-5 |
Ayyukan Tallafi
Za mu iya samar da ayyuka daban-daban na ginin ɗakin karatu na rigakafi bisa ga dabbobi da kuma ayyukan tantance ƙwayoyin cuta na halitta bisa ga buƙatun abokin ciniki.
Manufa Da Yawa
Akwai ayyukan gano ƙwayoyin cuta da dama da ake amfani da su wajen gano su: sunadarai, peptides, ƙananan ƙwayoyin cuta, ƙwayoyin cuta, sunadaran membrane, mRNA, da sauransu.
Vektoci da yawa
Sabis na musamman na ginin ɗakin karatu, za mu iya samar da nau'ikan vectors na bacteriophage daban-daban ciki har da PMECS, pComb3X, da pCANTAB 5E, da kuma gyara su bisa ga buƙatun abokin ciniki.
Tsarin Manyan Mutane
Ƙarfin ajiya zai iya kaiwa 10 ^ 8-10 ^ 9, ƙimar sakawa duk sun wuce 90%, kuma ƙarfin ƙwayoyin rigakafi da aka samu ta hanyar tantancewa gabaɗaya yana a matakin nM pM.
Sabis na Haɓaka Magungunan Hana Cututtukan Monoclonal
Za mu iya samar da ayyuka masu inganci, tsafta, da kuma takamaiman ayyuka na haɓaka ƙwayoyin cuta na monoclonal, gami da samar da ƙwayoyin rigakafi na monoclonal na linzamin kwamfuta da ƙwayoyin rigakafi na zomo na monoclonal
Dandalin Fasaha na Hybridoma
Ya haɗa da shirin rigakafi, ayyukan shirya antibody, tsarkake antibody, jerin abubuwan da antibody ke samarwa, tabbatar da antibody, da sauransu
Dandalin Rarraba Ƙwayar B Guda ɗaya
Alpha Lifetech tana da fa'idodi a lokacin tantancewa da kuma samun ingantattun ƙwayoyin rigakafi. Tana iya samar da ƙira, haɗawa, da gyarawa, garkuwar dabbobi, tantance haɓaka ƙwayoyin B guda ɗaya, da kuma jerin ƙwayoyin halitta guda ɗaya.
Dandalin Haɓaka Maganin Kariya Daga Fage Display Antibody
Alpha Lifetech na iya samar da ayyukan fasaha na haɓaka ƙwayoyin cuta na phage display antibody daga shirye-shiryen ƙwayoyin cuta, tsarkake ƙwayoyin cuta, jerin ƙwayoyin cuta, da sauransu.
Idan kuna da wasu tambayoyi, da fatan za ku iya tuntuɓar mu a kowane lokaci.
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2018-07-16 
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