Gabatarwa zuwa Dakunan karatu na Antibody Synthesized

Synthesized antibody lab, wanda kuma aka sani da ɗakin karatu na De novo, hanya ce ta roba wacce ke amfani da dabaru irin su haɗin DNA ko nunin phage don ƙira da haɗa cikakkun yankuna masu canzawa na antibody, gami da yankuna da CDRs, ba tare da dogaro da ɗakunan karatu na antibody ba.

Semi-synthetic antibody library an ƙirƙira shi ta hanyar haɗa ɗakunan karatu na antibody da ke faruwa a zahiri tare da bambancin roba. Yawanci ya ƙunshi haɗakar DNA oligonucleotides don samar da saiti na yankuna daban-daban na ƙayyadaddun ƙayyadaddun ƙayyadaddun bayanai (CDRs), waɗanda aka haɗa su tare da tsarin rigakafi na yau da kullun da aka samo daga tushen halitta kamar ƙwayoyin B na mutum ko dabba. Haɗaɗɗen CDR yana gabatar da bambance-bambance a cikin ɗakin karatu, yana haifar da ƙwayoyin rigakafi waɗanda ke yin niyya da yawa na epitopes. Laburaren antibody Semi-synthetic yana ba da daidaito tsakanin bambance-bambancen yanayin tsarin rigakafi da bambancin sarrafawa da aka samu ta hanyoyin roba.

Bambance-bambancen da ke tsakanin ɗakunan karatu na rigakafi da na roba ya dogara da tushen kwayoyin halittar immunoglobulin. A halin yanzu, bambamcin da ke tsakanin ɗakin karatu na wucin gadi da haɗakarwa ya ta'allaka ne a cikin cewa tsohon ya ƙunshi ɓangarori na naïve antibody segments, kamar sarƙar haske ko sarƙa mai nauyi, kuma ɗaya kawai daga cikinsu yana haɗa shi a cikin vitro; yayin da wanda aka haɗa gabaɗaya ya samo asali ne daga ƙirar wucin gadi ta PCR a cikin vitro.

Alpha Lifetech na iya bayarwa

Alpha Lifetech Inc. girmana iya ba da ɗakin karatu na ɗan lokaci-haɗaɗɗe / haɗakarwa daga dabbobi da mutane dangane da dandalin gano ƙwararrun mu. Tare da shekaru na gwaninta a cikin gyare-gyaren kwayoyin halitta da gina ɗakin karatu, za a iya gina ɗakunan karatu waɗanda ke da ikon samar da ƙwayoyin rigakafi na roba tare da dangantaka da ƙayyadaddun abubuwan da suka wuce ƙarfin ƙwayoyin rigakafi na halitta.Alpha Lifetech'smasana suna alfaharin sanar da cewa za mu iya ba da garantin babban rabo a cikin ginin ɗakin karatu na antibody mai ɗauke da 10^ 8 - 10 ^ 10 clones masu zaman kansu.

Alpha Lifetech Inc. girmayana alfahari da bayar da cikakkiyar sabis na ginin ɗakin karatu na antibody don biyan buƙatun masu bincike na duniya daban-daban, gami da scFv, Fab, VHH antibody, da ɗakunan karatu na musamman. Manufarmu ita ce fahimtar da biyan bukatun abokan ciniki daban-daban da kuma taimakawa tare da duk wata matsala mai zuwa da kuma tasowa a cikin aikin bincike.

Tsare-tsaren Sabis na Gina Laburaren Jikin Jiki

Zane
Tsarin ƙira ya ƙunshi zaɓin tsarin rigakafi da yankuna masu ƙayyadaddun ƙayyadaddun ƙayyadaddun bayanai (CDRs) dangane da jerin abubuwan rigakafin da ake da su ko bayanan tsari. Hakanan ana iya ƙirƙira ƙwayoyin rigakafin CDR na roba don gabatar da takamaiman ayyuka ko haɓaka abubuwan ɗauri.

Magana
DNA na roba da ke ɓoye ƙirƙira jeri na rigakafi, gami da yankuna biyu da CDRs, an haɗa su ta amfani da hanyoyin sinadarai ko enzymatic.

Majalisa
An haɗa gutsuttssun DNA ɗin da aka haɗe zuwa cikin sifofin maganganun antibody ta amfani da dabaru kamar PCR, ligation, ko taron Gibson. Ana iya shigar da waɗannan ƙwayoyin cuta cikin tsarin magana kamar ƙwayoyin cuta, yisti, ko ƙwayoyin dabbobi masu shayarwa don samar da maganin rigakafi.

Nunawa da Zaɓin
An duba dakunan karatu na antibody da aka gina kuma an zaɓi su don ƙwayoyin rigakafi tare da kaddarorin da ake so ta amfani da manyan hanyoyin tantancewa. Wannan na iya haɗawa da dabaru kamar nunin phage, nunin yisti, ko nunin ribosome, ya danganta da tsarin ɗakin karatu da aikace-aikace.

Sabis ɗin Gina Laburaren Ƙarƙashin Ƙarfafawa

Alpha Lifetech Inc. girmayana ba abokan ciniki ƙirar tasha ɗaya da haɗaɗɗun sabis na ɗakunan karatu na antibody ta hanyar haɗa nau'ikan nau'ikan nau'ikan nau'ikan nau'ikan V-gene masu nauyi da haske a cikin phage vector ta amfani da tsarin sake hadewa na musamman na Cre-lox don ƙirƙirar repertoire na Fabs wanda aka nuna akan phage wanda ya ƙunshi 6.5 × 10 ^ 10 clones. Laburaren ya samar da Abs daga antigens da yawa, wasu suna da alaƙar nanomolar. Ƙirƙirar ɗakin karatu na antibody ɗan adam ya dogara ne akan fasahar nunin phage M13 (wanda aka nuna a hoton da ke ƙasa).