Gabatarwa ga Laburaren Magungunan Haɗaka

Laburaren rigakafi da aka haɗa, wanda kuma aka sani da ɗakin karatu na De novo, hanya ce ta roba wadda ke amfani da dabaru kamar haɗa DNA ko nunin phage don tsara da kuma haɗa dukkan yankuna masu canzawa na rigakafi, gami da yankunan tsarin da CDRs, ba tare da dogaro da ɗakunan karatu na rigakafi marasa tsari ba.

Ana ƙirƙirar ɗakin karatu na rigakafi na Semi-synthetic ta hanyar haɗa ɗakunan karatu na rigakafi na halitta tare da bambancin roba. Yawanci yana haɗa da haɗa ƙwayoyin halittar DNA oligonucleotides don samar da saitin yankuna daban-daban masu ƙayyadewa (CDRs), waɗanda sannan ake haɗa su da tsarin rigakafi mai ɗorewa wanda aka samo daga tushen halitta kamar ƙwayoyin halittar ɗan adam ko dabba na B. CDR ɗin da aka haɗa yana gabatar da bambancin a cikin ɗakin karatu, wanda ke haifar da ƙwayoyin rigakafi da ke kai hari ga nau'ikan epitopes iri-iri. ɗakin karatu na rigakafi na semi-synthetic yana ba da sulhu tsakanin bambancin halitta na tsarin garkuwar jiki da bambancin da aka samu ta hanyar hanyoyin roba.

Bambancin da ke tsakanin ɗakunan ajiyar ƙwayoyin cuta na naïve da na roba ya dogara ne da tushen kwayoyin halittar immunoglobulin. A halin yanzu, bambancin da ke tsakanin ɗakin ajiyar ƙwayoyin cuta na semi-synthesized da synthesized antibody yana cikin cewa na farko ya ƙunshi wani ɓangare na sassan ƙwayoyin cuta na naïve, kamar sarkar haske ko sarkar nauyi, kuma ɗaya daga cikinsu ne kawai aka haɗa shi a cikin vitro; yayin da wanda aka haɗa gaba ɗaya an samo shi ne daga haɗin wucin gadi ta hanyar PCR in vitro.

Alpha Lifetech na iya samar da

Kamfanin Alpha Lifetech Inc.zai iya bayar da ɗakin karatu na ƙwayoyin cuta masu narkewa/haɗaka daga dabbobi da mutane bisa ga dandalin binciken ƙwayoyin cuta na ƙwararru. Tare da shekaru na gwaninta a gyaran kwayoyin halitta da gina ɗakin karatu, ana iya gina ɗakunan karatu masu haɗawa waɗanda za su iya samar da ƙwayoyin cuta masu narkewa waɗanda ke da alaƙa da takamaiman ƙarfin ƙwayoyin cuta na halitta.Alpha Lifetech'sMasana suna alfahari da sanar da cewa za mu iya tabbatar da babban nasarar da aka samu a gina ɗakin karatu na rigakafi wanda ke ɗauke da ƙwayoyin cuta masu zaman kansu 10^8 – 10^10.

Kamfanin Alpha Lifetech Inc.tana alfahari da bayar da cikakkun ayyukan gina ɗakin karatu na rigakafi don biyan buƙatun masu bincike na duniya daban-daban, gami da scFv, Fab, VHH antibody, da ɗakunan karatu na musamman. Manufarmu ita ce fahimtar da biyan buƙatun abokan ciniki daban-daban da kuma taimakawa wajen magance duk wata matsala da ke tafe da kuma tasowa a cikin aikin bincike.

Tsarin Ayyukan Gina Laburaren Magungunan Haɗaka

Zane
Matakin ƙira ya ƙunshi zaɓar tsarin rigakafi da yankuna masu ƙayyade rigakafi (CDRs) bisa ga jerin rigakafi ko bayanan tsarin rigakafi. Hakanan ana iya tsara ƙwayoyin rigakafi na CDR na roba don gabatar da takamaiman ayyuka ko haɓaka halayen ɗaurewa.

Tsarin
Ana haɗa DNA ɗin roba wanda ke tsara jerin ƙwayoyin rigakafi da aka tsara, gami da yankunan tsarin da CDRs, ta amfani da hanyoyin sinadarai ko enzyme.

Taro
Ana haɗa gutsuttsuran DNA ɗin da aka haɗa zuwa ƙwayoyin halittar bayyanar rigakafi ta amfani da dabarun kamar PCR, ligation, ko Gibson assembly. Sannan ana iya shigar da waɗannan ƙwayoyin halittar cikin tsarin bayyanar cututtuka kamar ƙwayoyin cuta, yisti, ko ƙwayoyin halittar dabbobi masu shayarwa don samar da ƙwayoyin rigakafi.

Nunawa da Zaɓe
Ana tantance ɗakunan ajiyar ƙwayoyin cuta na rigakafi da aka gina kuma a zaɓi su don ƙwayoyin rigakafi masu halaye da ake so ta amfani da hanyoyin tantancewa masu inganci. Wannan na iya haɗawa da dabarun kamar nunin phage, nunin yisti, ko nunin ribosome, ya danganta da tsarin ɗakin karatu da aikace-aikacensa.

Sabis na Gina Laburaren Magungunan Haɗaka

Kamfanin Alpha Lifetech Inc.yana ba wa abokan ciniki ƙira ɗaya da sabis na haɗakar dakunan karatu na rigakafi ta hanyar haɗa manyan nau'ikan V-gene masu nauyi da haske zuwa vector na phage ta amfani da tsarin sake haɗawa na musamman na wurin Cre-lox don ƙirƙirar tarin Fabs da aka nuna akan phage wanda ya ƙunshi kwafi 6.5 × 10^10. Laburaren ya samar da Abs akan antigens da yawa, wasu tare da alaƙar nanomolar. Samar da ɗakin karatu na rigakafi na ɗan adam ya dogara ne akan fasahar nunin phage na M13 (wanda aka nuna a cikin hoton da ke ƙasa).