Hoʻolauna ʻana i ka lawelawe hōʻike Phage

ʻO ka hōʻike ʻana o Phage, ma ke ʻano he ʻenehana molekala e pili ana i ka hoʻololi genetic o ka phage DNA, ua lilo ia i mea hana ikaika molekala e hana i nā probes molekala e kūʻē i nā pahuhopu kikoʻī ma ka hōʻike ʻana i kēia mau ʻāpana ma ka ʻili phage a pēlā e koho ai i nā ʻāpana peptide/antibody me nā waiwai hoʻopaʻa kikoʻī mai kahi nui o nā ʻano like ʻole (nā hale waihona puke).

He ʻumi makahiki o ka ʻike o Alpha Lifetech i ka hoʻomohala ʻana i nā antibodies hōʻike phage a hiki iā ia ke hāʻawi i ke kūkulu ʻana i ka hale waihona puke antibody ʻoihana a me ka lawelawe nānā no kā mākou mea kūʻai aku. Wahi a nā pono o nā mea kūʻai aku, hiki iā mākou ke hāʻawi i nā ʻano like ʻole o nā hale waihona puke antibody, e like me nā hale waihona puke immune, nā hale waihona puke kūloko, nā hale waihona puke synthetic a me nā hale waihona puke semi-synthetic. ʻO ka ʻenehana hale waihona puke hōʻike phage, ka mea e hoʻohui i nā genes antibody me nā genes o nā protein phage coat e like me M13, ʻo ia ka ʻenehana hoʻomākaukau antibody recombinant maʻamau a hoʻohana nui ʻia i ka hoʻomohala ʻana i nā antibodies kikoʻī loa.

E hoʻopili ʻo Alpha Lifetech i ka alpaca, llama, alpaca, a me nā mano, a pēlā aku, i hiki ai i ko lākou pono ke piʻi i ka 10^5. E hoʻouna mākou i ka hōʻike hoʻāʻo pono i ka mea kūʻai aku e hōʻoia i ka pono a me ka ʻoiaʻiʻo o ka ʻikepili. Loaʻa i nā hale waihona puke kūloko nā pono o ka haʻahaʻa o ka ʻawahia a me ka haʻahaʻa o ka immunogenicity. Loaʻa iā Alpha Lifetech kahi nui o nā hale waihona puke antibody kūloko i hana mua ʻia, hiki ke hoʻohana pololei ʻia no ka nānā ʻana i ka antibody me ka ʻole o ka hoʻopili ʻana i nā holoholona, a pēlā e hoʻopōkole ai i ka manawa o ka papahana, e hoʻolaha ana i ka holomua o ka hoʻāʻo ʻana o ka mea kūʻai aku, a e hōʻoiaʻiʻo ana i ka pono o ka hana o ka antibody.

ka ʻike ʻana o ka antibody-Alpha Lifetech

Kiʻi 1: Kumumanaʻo hōʻike Phage

ʻO kā mākou ʻōnaehana hōʻike Phage

Ua hōʻike ʻia ka ʻike nui o ka ʻōnaehana hōʻike phage M13/T4/T7 ma ka Papa 1.

Papa 1 Nā kaʻa hōʻikeʻike phage like ʻole a me ko lākou mau ʻano
	M13	T4	T7
Ka nui o ka genome	6407 bp	168895 bp	39937 bp
Hōʻike i ka Protein	pVI, pIII a me pVIII	SOC a me HOC	gp10B
Ka nui o ka hōʻike	>110 kDa ʻo ia ka pIII
Ka nui o ka hōʻike
Hōʻike i ka nui
Hōʻike i ka nui
Pōʻaiapuni ola	Lisogeny	Lytic	Lytic

Hiki iā Alpha Lfetech ke hāʻawi

Hoʻohui ʻo Phage Display i ka genotype a me ka phenotype i hoʻokahi, e hoʻohui ana i ke koho ʻana a me ka hoʻonui ʻana me nā hiki ke nānā ikaika. Hiki iā Alpha Lifetech ke hāʻawi i kahi ākea o nā lawelawe no ka hoʻomohala ʻana i ka antibody phage display.
ʻO nā lawelawe nui e komo pū ana: ka paepae waihona puke antibody VHH, ka paepae waihona puke antibody scFv, ka paepae waihona puke antibody Fab, ka paepae kūkulu waihona puke phage, ka paepae nānā waihona puke phage a me ka lawelawe hoʻokanaka antibody a me nā lawelawe ʻē aʻe.

ka hoʻomohala ʻana o ka antibody-Alpha Lifetech

Kiʻi 2: Ke kaʻina hana lawelawe hoʻomohala hōʻike Phage

Kaʻina Hana Hoʻomohala Antibody Hōʻike Phage

Hiki iā mākou ke pale aku i nā holoholona me nā protein i hōʻike ʻia i loko o kā mākou keʻena hoʻokolohua a i hoʻolako ʻia e kā mākou mea kūʻai aku, me nā protein macromolecular like ʻole, peptides a me nā protein membrane. Ma o ka hoʻāʻo pono ʻana o ELISA, ua hana kā mākou poʻe noiʻi i ka hoʻokaʻawale ʻana o PBMC a me ka unuhi ʻana o RNA mai ke koko o nā alpacas me nā hopena pale maikaʻi loa. Ma o ka transcription reverse a me ka hoʻonui ʻana o ka gene, ua kūkulu mākou i ka gene ma nā vectors phage pMESC, pComb3XSS a i ʻole pCANTAB 5E. Ma o ka electrotransformation o nā cell competent TG1 E. coli, hiki ke loaʻa nā hale waihona puke antibody phage me ka hiki ke waihona puke ma mua o 10^9. Loaʻa i nā hale waihona puke antibody me ka hiki ke waihona puke nui ka ʻokoʻa maikaʻi a kōkua i ka loaʻa ʻana o nā antibodies me ka affinity. No ka hōʻoia ʻana i ka maikaʻi o ka hale waihona puke antibody, e wehewehe mākou i ka hiki ke waihona puke a me ka helu hoʻokomo o ka hale waihona puke. Hoʻonohonoho hope mākou i nā hale waihona puke e hāʻawi i kā mākou mea kūʻai aku me nā moʻo 30-50 e pono ai. A laila, hana mākou i ka nānā ʻana o ka waihona hōʻike phage, ʻO ka mea mua, ua hoʻopaʻa ʻia ka antigen ma nā microplates polypropylene, a ma o 3-5 mau puni o ka nānā ʻana, ua wehe ʻia nā phage-antibodies me ka hiki ke nakinaki nāwaliwali, a ua mālama ʻia nā clones kikoʻī e nakinaki ana i ka antigen. No ka hōʻike ʻana o ka phage, hoʻohana ʻia ke ʻano ELISA e hoʻopau me kahi clone maikaʻi, ʻoiai no ka hōʻike ʻana o ka hū, hoʻohana ʻia ke ʻano FACS e hōʻike i nā cell i nā ʻāpana antibody recombinant me ka hoʻohana ʻana i nā antigens i lepili ʻia. Ma o ka nānā ʻana, hoʻomākaukau mākou i nā antibodies me ke ʻano kiʻekiʻe, ke kikoʻī a me ka pilina kiʻekiʻe.

Kiʻi 3 Hōʻikeʻike Phage i ke kaʻina hana hoʻohuihui antibody

Nā Pōmaikaʻi o ka Lawelawe
Nā pono o ka lawelawe

Ua kūpaʻa nui ʻo Alpha Lifetech i ka ʻenehana hōʻike phage, a hiki ke hāʻawi i nā mea kūʻai aku me nā lawelawe phage ākea a ʻoi aku ka maikaʻi.

Ka manawa a me ka pono kiʻekiʻe

Hiki i kā mākou hui ke ʻimi i nā antibodies kūpono i ka manawa pōkole; hiki iā mākou ke hoʻokō i nā hoʻokolohua loiloi antibody like ʻole e like me nā pono o nā mea kūʻai aku.

Kūlana huahana kiʻekiʻe

Kōkua kā mākou hui i nā mea kūʻai aku e hoʻomākaukau i nā antibodies me ke kikoʻī kiʻekiʻe a me ke kūpaʻa, e hoʻonui ana i ka hopena o nā hopena noiʻi ʻepekema.

Hāʻawi i nā koho like ʻole

Hiki i kā mākou hui ke kūkulu i nā hale waihona puke antibody multi-ʻano (kanaka, ʻiole, lāpaki, alpaca, a me nā mea ʻē aʻe) a hiki ke kūkulu i nā ʻano like ʻole (scfv, Fab, VHH, a me nā mea ʻē aʻe) o nā hale waihona puke antibody.

Ka Mana Nui

Loaʻa i kā mākou waihona puke antibody kahi mana nui o nā antibodies ma mua o 10^9

Nā nīnau i nīnau pinepine ʻia - Nā pilikia laulā o ka hōʻike ʻana o ka phage

Nā nīnau hōʻike phage ◢

Q.
He aha ka ʻenehana hōʻike phage?

A.
ʻO ka ʻenehana hōʻike Phage ka hoʻokomo ʻana i ka gene target haole i loko o ke kūlana kikoʻī o ka protein phage capsid, a hōʻike i ka gene haole ma luna o ka ʻili o ka phage me ka ʻole o ka hoʻopilikia ʻana i ka hana maʻamau o ka phage. Ma hope o kekahi mau pōʻai o ka nānā ʻana, ua loaʻa hope ka protein target me ka kikoʻī ikaika a me ka hana olaola kiʻekiʻe.
Q.
He aha nā pōmaikaʻi o ka ʻenehana hōʻike phage?

A.
Hiki ke hoʻokumu ʻia nā waihona antibody kiʻekiʻe me ka hoʻohana ʻana i kēia ʻenehana. He pōkole ke kaʻina hana nānā, he maʻalahi ke kaʻina hana, a he ākea ka laulā o nā noi, e ʻae ana i ka ʻike ʻana i nā kaʻina kūpono no nā peptides a i ʻole nā protein i kuhikuhi ʻia. I nā noi e pili ana i ka virus, hiki i nā mea noiʻi ke ʻike i nā antibodies e hoʻopaʻa i nā epitopes viral koʻikoʻi ma ka nānā ʻana i nā waihona antibody nui. Hiki i kēia kaʻina hana ke pale i ke komo ʻana, ka hana hou ʻana, a i ʻole nā ʻano pale ʻana o nā virus, a laila e hoʻokumu ana i kahi kahua no ka mālama ʻana i nā maʻi.
Q.
He aha ka ʻokoʻa ma waena o ka ʻenehana hybridoma a me ka ʻenehana phage?

A.
ʻOi aku ka hoʻohana nui ʻia o ka ʻenehana hōʻike phage ma mua o ka ʻenehana hybridoma, kahi i kaupalena ʻia i ka hana ʻana i kahi liʻiliʻi o nā antibodies e kūʻē i nā immunogens kikoʻī. I ka hoʻohālikelike ʻana, hiki i ka ʻenehana hōʻike phage ke hōʻike i kahi waihona antibody holoʻokoʻa i loaʻa mai nā ʻano ʻano pale like ʻole.
Q.
He aha nā hoʻohana o ka ʻenehana hōʻike phage?

A.
He ākea nā noi o ka ʻenehana hōʻikeʻike phage, me ka nānā ʻana i nā mea loaʻa o nā microorganism pathogenic, ke aʻo ʻana i nā pilina protein, ka nānā ʻana i ka hoʻoili ʻana o nā hōʻailona ma waena o nā cell, ka ʻike ʻana i nā maʻi holoholona, a me ka hoʻomohala ʻana i nā lāʻau pale, ma waena o nā ʻoihana ʻē aʻe.
Q.
Ka manaʻolana o ka ʻenehana hōʻike phage?

A.
Hāʻawi ka ʻenehana hōʻike phage i nā pono he nui a hiki ke hoʻohana ʻia ma nā ʻano like ʻole. Eia nō naʻe, ʻo ka pilikia nui i ka hoʻomohala ʻana i nā antibodies recombinant me ka hoʻohana ʻana i ke ʻano hōʻike phage ʻo ia ka paʻakikī a me ke kumukūʻai e pili ana i ke kaʻina hana. Pono kēia ʻano hana i ka hoʻohui ʻana o nā ʻenehana biology molecular, me ka mutation, cloning, a me ka expression, me nā immunological, biochemical, a me nā loiloi biophysical e ʻike a wehewehe i nā antibodies i makemake ʻia.

Nā nīnau e pili ana i ka antigen ◢

Q.
He aha nā ʻano hana e hōʻike ai i nā antigens?

A.
He loulou koʻikoʻi loa ka hōʻike ʻana o ka antigen. ʻO nā ʻano o ka hōʻike ʻana o ka antigen e komo pū me ka hōʻike pololei ʻana o ka antigen i uhi ʻia, ka hōʻike ʻana o ka antigen i uhi ʻia ʻole, ka hōʻike ʻana o ka antigen ma o ka nānā ʻana o nā cell holoʻokoʻa, ka hōʻike ʻana o ka antigen ma o nā liposomes, nā nano-phospholipid disks a me nā VLP.
Q.
He aha nā ʻokoʻa ma waena o nā ʻano like ʻole o ka hōʻike ʻana o nā antigen?

A.
He maʻalahi ke ʻano o ke uhi pololei ʻana, akā maʻalahi ke hoʻololi i ke ʻano antigen, a he paʻakikī ka hana uhi pololei ʻole, akā hiki iā ia ke mālama i ke ʻano antigen a hoʻomaikaʻi i ka pono o ka hōʻike ʻana. Hiki i ka nānā ʻana o ke kelepona ke hoʻohālike i ke kahua maoli i loko o ke kino, akā aia kahi pilikia o ka hoʻopaʻa ʻole kikoʻī. He liʻiliʻi ka nui o ka disc Nano-phospholipid, kiʻekiʻe ka pono o ka lawe ʻana a me ke kūpaʻa maikaʻi; Hoʻohui ʻia nā VLP me ka antigen a i ʻole i kāʻei ʻia me ka antigen, nona ka immunogenicity kiʻekiʻe a me ka palekana maikaʻi, a hiki ke hoʻoulu i kahi pane pale ikaika.
Q.
He aha ka hopena o ka nui o ka antigen ma nā hopena nānā o ka ʻenehana hōʻike phage?

A.
Inā haʻahaʻa loa ka nui o ka hoʻohuihui ʻana, e emi ana ka likelika o ka hoʻopaʻa ʻana me ka phage, e hopena ana i ka emi ʻana o ka pono o ka pale ʻana. I ka ʻaoʻao ʻē aʻe, hiki i kahi nui o ka hoʻohuihui kiʻekiʻe loa ke hoʻonui i ka likelika o ka hoʻopaʻa ʻana i nā mea ʻaʻole kikoʻī, hoʻokiʻekiʻe i ka hōʻailona hope, a keakea i ka ʻike ʻana o nā clones maikaʻi.
Q.
Pehea e hiki ai iā mākou ke hōʻoia i ka mālama ʻana o ka antigen i kona ʻano kūlohelohe i ka wā o ke kaʻina hana nānā?

A.
Hiki ke hoʻohana ʻia ke ʻano hoʻopaʻa akahai e pale aku i ke kiʻekiʻe o ka mahana, ka waikawa ikaika a me ka alkali. Ua hoʻohana ʻia ke ʻano uhi ʻana ʻaʻole pololei a me ka ʻōnaehana biotin-streptavidin e hōʻemi i ka hōʻino ʻana i ke ʻano antigen. Inā he protein membrane ka antigen, hiki ke hōʻike ʻia ma luna o ka ʻili o nā cell i hoʻopaʻa ʻia a hoʻohana ʻia paha e hoʻohālike i ke kaiapuni membrane, e like me ka nano-disk, e mālama i kona ʻano protein membrane kūlohelohe.
Q.
Pehea e hōʻoia ai i ke ʻano kikoʻī o ka hoʻopaʻa ʻana o nā antibodies i nānā ʻia e ka ʻenehana hōʻike phage i nā antigens?

A.
Ma waho aʻe o nā hoʻokolohua maʻamau e like me ELISA, Western Blotting (WB), a me Immunofluorescence (IF), hiki ke hoʻohana ʻia nā ʻano hana ʻike ʻo Surface Plasmon Resonance (SPR) a me Bio-Layer Interferometry (BLI) e nānā i nā kaʻina hana hoʻopaʻa a me ka dissociation o nā antibodies a me nā antigens i ka manawa maoli, e ʻae ana i ka hoʻoholo ʻana i nā ʻano affinity a me kinetic.

Nā Nīnau e pili ana i ka pale ʻana o ka maʻi ◢

Q.
He aha nā pane palekana maʻamau o nā holoholona i ka ʻenehana hōʻike phage?

A.
Hiki ke hoʻohana ʻia ka hōʻike ʻana o Phage e hana i nā antibodies monoclonal affinity kiʻekiʻe no nā ʻano ʻano hana antibody, me ka palena ʻole i nā kānaka, nā ʻiole, nā ʻiole, nā lāpaki, nā moa, nā kāmelo, nā alpacas, nā bipi wahine, nā ʻīlio, nā hipa, nā mōneka, a me nā manō.
Q.
He aha nā ʻano a me nā hiʻohiʻona kūpono o nā hale waihona puke antibody i loaʻa mai nā ʻano holoholona like ʻole i ka ʻenehana hōʻike phage?

A.
Ua hoʻokumu maikaʻi ʻia ka ʻenehana kūkulu ʻana o nā waihona antibody ʻiole, kūpono i ke kumukūʻai, a he pōkole ka manawa hoʻokolohua, e kūpono ai no ka nānā mua ʻana i ka antibody a me ka noiʻi kumu. Hōʻike nā antibodies rabbit i ka pilina ikaika a me ke kikoʻī, me kahi ākea o ka ʻike epitope i hoʻohālikelike ʻia me nā antibodies ʻiole. Hiki ke loaʻa pololei nā antibodies kanaka mai nā waihona antibody kanaka, no laila e pale aku ai i nā hopena pale kino e hiki mai ana ke hāʻawi ʻia nā antibodies heterologous i ke kino kanaka. He waiwai nui kēia ʻano hana i ka noiʻi a me ka hoʻomohala ʻana i nā lāʻau lapaʻau, ʻoi aku hoʻi i ka hana ʻana i nā antibodies therapeutic.
Q.
He aha ka immunogen holoholona maʻamau i hoʻohana ʻia i ka ʻenehana hōʻike phage?

A.
Ma ke ʻano laulā, ua māhele ʻia nā immunogens holoholona i ka antigen protein, ka antigen polypeptide, ka antigen nucleic acid a me ka antigen e pili ana i ka pathogen, ma waena o ia mau mea, ʻo ka antigen e pili ana i ka pathogen e komo pū me ka antigen virus (ʻāpana virus piha, protein virus, protein non-structural virus, a me nā mea ʻē aʻe), antigen bacteria (ʻāpana bacteria piha, ʻāpana paia cell, protein huna, toxin, a me nā mea ʻē aʻe) a me ka antigen parasite.
Q.
Pehea e hiki ai iā mākou ke hoʻoponopono i ka pilikia o ka hoʻomanawanui o ka pale ʻana i nā holoholona i ka wā o ka pane pale?

A.
E hoʻoponopono i nā immunogens ma ke pani ʻana i nā antigens immune me nā protein recombinant a me ke pani ʻana i nā ʻāpana virus e kinai ahi me nā protein hoʻonohonoho o nā virus. E hoʻoponopono i ka hoʻolālā hoʻokolohua a hoʻololi i ka dosage immune.
Q.
Pehea e hoʻomaikaʻi ʻia ai ka immunogenicity o nā immunogens i ka ʻenehana hōʻike phage?

A.
Hiki ke hoʻonui ʻia ka immunogenicity ma o ka hoʻopili ʻana i nā antigens me nā protein lawe, e like me ka bovine serum albumin. Eia kekahi, hiki ke hoʻohana ʻia nā adjuvants immune, me ka adjuvant Freund a me ka adjuvant alumini, e hoʻolōʻihi i ka manawa noho in vivo a hoʻoulu i ka hoʻoulu ʻana a me ka hoʻonui ʻana o nā cell immune, no laila e hoʻomaikaʻi ai i ka immunogenicity.

Nā Nīnau Kūkulu Hale Waihona Puke ◢

Q.
He aha ka waihona puke antibody phage?

A.
ʻO kahi waihona puke antibody phage kahi hōʻiliʻili o nā antibodies phage like ʻole i hana ʻia ma ka hōʻuluʻulu ʻana i kahi hoʻonohonoho piha o nā genes ʻāpana loli mai nā antibodies i loko o nā vectors phage a hōʻike iā lākou ma nā ʻili o nā phages. ʻO kēia kaʻina hana ka hopena i ka hoʻokumu ʻia ʻana o kahi waihona puke antibody phage.
Q.
Ke kaʻina hana o ke kūkulu ʻana i kahi waihona puke antibody phage

A.
ʻO ke kaʻina hana maʻamau o ka hōʻike ʻana i ka phage e pili ana i kekahi mau ʻanuʻu koʻikoʻi: ka unuhi ʻana i nā pūnaewele mononuclear koko peripheral (PBMC) mai nā holoholona i hoʻopili ʻia, ka hoʻokaʻawale ʻana i ka RNA holoʻokoʻa a me ke kākau ʻana iā ia i loko o ka DNA complementary (cDNA), ka hoʻonui ʻana i ka gene target me ka hoʻohana ʻana i ka ʻenehana polymerase chain reaction (PCR), ke cloning ʻana i ka gene target i loko o kahi vector phage, a me ka hoʻololi ʻana i ka vector i loko o kahi cell host no ka hōʻike ʻana. ʻO kēia kaʻina hana ka hopena i ke kūkulu ʻana i kahi waihona hōʻike phage, hiki ke ʻike koke i nā antibodies affinity kiʻekiʻe. Ma hope mai, hiki i kēia waihona ke lawelawe ma ke ʻano he kumuwaiwai waiwai nui no ka hoʻomohala ʻana i nā lāʻau pale, nā lāʻau lapaʻau, a me nā reagents diagnostic.
Q.
He aha nā kumu nui o ke kūkulu ʻana i kahi hale waihona puke maikaʻi?

A.

E hōʻoia i ka lawa ʻana o ka huina o PBMC. E hōʻoia i ka maikaʻi o ka RNA me ka ʻole o ka hōʻino ʻia.

No ka hōʻoia ʻana i ka huina o nā mea hoʻololi, pono ka mana o ka waihona puke he 10 a 100 mau manawa o ka ʻokoʻa kumumanaʻo.
Q.
He aha nā hoʻokaʻawale ʻana o ka waihona puke antibody phage?

A.
Wahi a ke kumu o ka waihona puke antibody, hiki ke hoʻokaʻawale ʻia nā waihona puke antibody phage i nā waihona puke antibody phage immune a me nā waihona puke antibody phage kūloko. ʻO ka ʻokoʻa nui ma waena o kēia mau ʻano ʻelua aia i ka wā i hoʻopili ʻia ai nā holoholona. Eia kekahi, hiki ke hoʻopuka ʻia nā waihona puke antibody kūloko ma o ka semi-synthesis a i ʻole synthesis.
Q.
Pehea e hiki ai i kekahi ke loiloi i ka maikaʻi o kahi waihona puke antibody phage?

A.
ʻElua mau ʻōkuhi mua no ka loiloi ʻana i ka maikaʻi o kahi waihona puke antibody: ka hiki ke mālama a me ka ʻokoʻa. ʻO kahi hiki ke mālama nui, i hui pū ʻia me ke ʻano like ʻole kiʻekiʻe, e hōʻoiaʻiʻo ana i ka nānā pono ʻana o nā antibodies me ka affinity kiʻekiʻe a me ke kikoʻī.

Nā Nīnau Nānā Hale Waihona Puke ◢

Q.
He aha ke kaʻina hana o ka nānā ʻana i kahi waihona puke antibody phage?

A.
I ke kaʻina hana o ka elutriation biological, ua hoʻoulu ʻia ka waihona hōʻikeʻike me nā pahuhopu paʻa a laila holoi nui ʻia e wehe i nā phages non-reactive. Hoʻoheheʻe pinepine ʻia nā Conjugates me ka hoʻohana ʻana i kahi hopena acidic a i ʻole ka paʻakai kiʻekiʻe a ma hope e hoʻonui ʻia i loko o nā cell host kūpono no ka hoʻonui ʻana. Ma hope o 3 a 5 mau pōʻai o ka nānā ʻana, loaʻa nā antibodies me ka affinity kiʻekiʻe.
Q.
He aha nā ʻano hana i hoʻohana ʻia no ka nānā ʻana i nā hale waihona puke antibody phage?

A.
ʻO nā ʻano hana nānā phage e komo pū me ka panning pae paʻa, ka nānā ʻana i ka pae wai, a me ka nānā ʻana ma muli o ke kelepona. Pono ke koho ʻana i kahi ʻano nānā kūpono e noʻonoʻo i nā ʻano o nā molekala i manaʻo ʻia, nā pahuhopu o ke kaʻina hana nānā, a me ke kaiapuni hoʻokolohua.
Q.
He aha nā loiloi maikaʻi a maikaʻi ʻole o nā phages?

A.
ʻO ka nānā maikaʻi ke ʻano kumu loa no ka nānā ʻana i nā antibodies phage e hoʻopaʻa i nā protein target. Hana ka nānā maikaʻi ʻole e hoʻopau i nā antibodies nonspecific. I ka wā o ke kaʻina hana nānā antibody phage, hiki ke ʻike ʻia nā antibodies nonspecific ma muli o ke keakea ʻana mai ka lepili antigen target, nā mea hana, a i ʻole nā antigens ʻē aʻe me nā ʻano like me ka antigen target. I ia mau hihia, hiki i ka nānā maikaʻi ʻole ke hoʻonui i ka pono o ke kaʻina hana nānā.
Q.
Pehea e pale aku ai i ka nānā ʻana i nā antibodies nonspecific?

A.

Hiki ke hoʻonohonoho mua ʻia nā antigens i lepili ʻia me nā ʻano māka like ʻole no ka nānā mua ʻana, a i ʻole hiki ke hoʻohana ʻia ka nānā maikaʻi ʻole e hoʻemi i ka hapa o nā antibodies kikoʻī ʻole.

Hiki iā ʻoe ke koho e pani i ka wai sila kūpono a i ʻole e hoʻohana i nā wai sila like ʻole e pale aku i kēia kūlana. Inā nānā ʻia ka antibody e ʻike ana i ka laina cell hope (CHO a i ʻole HEK293), hiki iā ʻoe ke pani i nā laina cell hope like ʻole no nā kumu nānā a i ʻole ka nānā maikaʻi ʻole.
Q.
Pehea e hiki ai i kekahi ke hōʻoia a kālailai i nā antibodies i loaʻa?

A.
ʻO nā ʻano hana no ka hōʻoia ʻana i ka antibody e komo pū me ELISA, Western Blotting (WB), flow cytometry, immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, a me nā ʻano hana hoʻokolohua like ʻole. Loaʻa iā Alpha Lifetech ka ʻike nui i ka ʻike ʻana i ka antibody a he hui hoʻokolohua ʻoihana ko ia, e hōʻoiaʻiʻo ana i ka pono o nā antibodies i nā hoʻokolohua ma hope.

Inā he mau nīnau kāu, e ʻoluʻolu e hoʻokaʻaʻike mai iā mākou i kēlā me kēia manawa.

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Kahua ʻIke Antibody