Kahua Hana ʻo Aptamer
Ma muli o ka pahuhopu, ʻo nā aptamers waikawa nucleic e komo pū me nā aptamers DNA, nā aptamers RNA, nā aptamers protein, a me nā aptamers membrane cell.
KĀHEA IĀ MĀ˚OU01
Kahua Hana ʻo Aptamer
Hoʻolauna i nā aptamers waikawa Nucleic
ʻO nā aptamers waikawa nucleic, i ʻike ʻia hoʻi he aptamers, complexes, a me nā antibodies kemika, loaʻa ma o ka nānā ʻana o ka selex in vitro e ka ligand exponential enrichment system evolution (SELEX) technique. He deoxyribonucleic acid (ssDNA) a i ʻole ribonucleic acid (RNA) hoʻokahi kaula e hoʻopaʻa pono a me ka maikaʻi i kahi mole i manaʻo ʻia, a ʻo ka maʻamau he 20-80 mau kumu.
ʻO nā aptamers waikawa nucleic i loaʻa ma o ka nānā ʻana in vitro he paʻa ke ʻano, maʻalahi e synthesise a maʻalahi hoʻi e hoʻololi, a ʻoi aku ka maikaʻi ma mua o nā antibodies i loaʻa mai ka biologically i hoʻohana pinepine ʻia ma ke ʻano o ka synthesis a me ka noi; eia kekahi, hiki i nā aptamers waikawa nucleic ke hoʻopaʻa pono i kā lākou mau pahuhopu ma o nā ʻano ikaika like ʻole, ma ke ʻano laulā, launa pū lākou me nā mole liʻiliʻi e pili ana i kahi ākea o nā conformations (e laʻa, hairpins, concave-convex, pseudo-twisted, etc.) ma o ka hydrogen bonding, electrostatic forces, hydrophobic interactions, etc. molecules. Ma muli o ka pahuhopu, ʻo nā aptamers waikawa nucleic e komo pū me nā aptamers DNA, nā aptamers RNA, nā aptamers protein, a me nā aptamers membrane cell.
ʻO ka paepae synthesis aptamer a Alpha Lifetech, ka mea nui e pili ana i ka lawelawe synthesis waihona puke aptamer SELEX a me ka lawelawe hoʻomohala aptamer (DNA, RNA a i ʻole XNA), a me ka nānā ʻana o ka aptamer ma lalo, ka hoʻonui ʻana i ka aptamer, a me nā lawelawe loiloi ʻike aptamer.
Kaʻina hana loea o ka hoʻohui ʻana o Aptamer
Kumumanaʻo Kumu a me ke Kaʻina Hana ʻenehana
ʻO ke kaʻina hana o ka ʻenehana synthesis nucleic acid aptamer ʻo ia ka hoʻohuihui kemika ʻana o kahi waihona puke o nā oligonucleotides kaula hoʻokahi i loko o ka vitro, i hui pū ʻia me ka mea i manaʻo ʻia, a aia kahi paʻakikī o ka mea i manaʻo ʻia a me ka waikawa nucleic i loko o ka hui ʻana, holoi ʻia ka waikawa nucleic i hoʻopaʻa ʻole ʻia i ka mea i manaʻo ʻia, a hoʻokaʻawale ʻia nā molekala waikawa nucleic e hoʻopaʻa ana i ka mea i manaʻo ʻia, a hoʻohana ʻia nā molekala waikawa nucleic ma ke ʻano he template e hana i ka hoʻonui PCR no ka pōʻai aʻe o ka nānā ʻana. Ma o ka nānā ʻana a me ka hoʻonui ʻana o ka selex hou ʻia, e holoi ʻia kekahi mau molekala DNA a RNA paha i hoʻopaʻa ʻole ʻia i ka mea i manaʻo ʻia a i ʻole he haʻahaʻa a haʻahaʻa paha no ka mea i manaʻo ʻia, a e hoʻokaʻawale ʻia nā aptamers, ʻo ia hoʻi, DNA a RNA paha me ka pilina kiʻekiʻe no ka mea i manaʻo ʻia, mai ka waihona puke nui loa, a e hoʻonui ʻia ko lākou maʻemaʻe me ke kaʻina hana SELEX, a i ka hopena e noho lākou i kahi hapa nui o ka waihona puke.
Nā Manaʻo Koʻikoʻi o ke Kaʻina Hana ʻenehana
Hoʻomaka ke kaʻina hana o ka nānā ʻana o ka selex o nā aptamers nucleic acid me ka hōʻuluʻulu ʻana o nā hale waihona puke oligonucleotide random i hana ʻia me ke kemika. Loaʻa i nā hale waihona puke oligonucleotide random kahi mana nui o ka hale waihona puke. ʻO ke ʻano like ʻole kiʻekiʻe kona pono nui. Ma ke kumumanaʻo, inā ʻo ka moʻo random i loko o kahi oligonucleotide he n mau kumu, ʻo ka mana o ka hale waihona puke he 4n. Inā mākou e noʻonoʻo i ka hale waihona puke hoʻololi artificial, e hoʻonui ia i ka ʻokoʻa o nā moʻo random. I kēia manawa, ʻo ka lōʻihi o nā moʻo random o nā oligonucleotides i hoʻohana pinepine ʻia i nā hoʻokolohua SELEX he 30 mau kumu, a hiki i ka mana o ka hale waihona puke ke kiʻekiʻe e like me 10^18. He mea koʻikoʻi ka mana o ka hale waihona puke no ka nānā ʻana o ka selex o nā ligands affinity kiʻekiʻe. Eia naʻe, no ka mea ua maopopo ka nui o nā hale waihona puke synthetic, ʻo nā moʻo random lōʻihi loa e hōʻemi i ka nui o kēlā me kēia oligonucleotide i loko o nā hale waihona puke, kahi e hōʻemi ai i ka hiki ke nānā ʻana o ka selex no kahi aptamer kikoʻī. Ma keʻano laulā, hiki i ka mana o ka hale waihona puke o 10^13~10^15 i ka pōʻai mua o ka nānā ʻana o ka selex ke hoʻokō i nā pono hana.
Kiʻi 1 Kaʻina Hana ʻenehana SELEX
nā pono o ka aptamer waikawa Nucleic
| Nā Pōmaikaʻi | Nā kikoʻī |
|---|---|
| Pololei | Ke pale nei i ke kaʻina hana kuʻuna o nā hoʻokolohua holoholona a me ke koho pololei ʻana mai nā hale waihona puke in vitro; ka nānā ʻana o selex no nā aptamers o nā molekala i manaʻo ʻia he non-immunogenic, low-immunogenic a i ʻole ka ʻawahia. |
| Ka laulā o nā noi | Hiki i nā molekala i makemake ʻia ke lilo i nā mea hoʻonaninani organik, nā waikawa amino, nā protein, nā antibiotics, nā peptides, nā huaora, nā lāʻau lapaʻau, ʻoiai nā cell, nā pathogens, nā virus, nā ʻiʻo, a me nā mea ʻē aʻe. |
| Pilina ikaika | Hōʻike nā Aptamers i ke kikoʻī kiʻekiʻe a me ka pilina no ka nānā ʻana in vitro o nā molekala target. |
| Paʻa | He liʻiliʻi ka nui o nā Aptamers, maʻalahi ke loaʻa, hiki ke hana hou ʻia i ka synthesis, a hiki ke hoʻonui ʻia ko lākou kūpaʻa e ka synthesis kemika a me ka hoʻololi ʻana. He paʻa kemika nā aptamers, he lōʻihi ko lākou ola papa a hiki ke lawe ʻia i ka mahana o ka lumi. |
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Lāʻau lapaʻau: ka mālama ʻana i ka maʻi ʻaʻai i kuhikuhi ʻia, nā ʻano hana hōʻoia maʻi kanesa
Ua kūkulu pono ʻia nā hōʻike hōʻaiʻē o Gladtrust ma ke kumu o ka waihona ʻikepili hōʻaiʻē ʻoihana pūnaewele nui loa - CreditVision.
Hōʻiliʻili ka waihona ʻikepili i ka manawa kūpono a hōʻano hou i ka ʻike o nā kumu ʻikepili ma mua o 200,000. Hiki i ke kaʻina hana hoʻonui a me ka hoʻokele ʻike ke hōʻoia i ka pololei o ka ʻike.
Hōʻiliʻili ka waihona ʻikepili i ka manawa kūpono a hōʻano hou i ka ʻike o nā kumu ʻikepili ma mua o 200,000. Hiki i ke kaʻina hana hoʻonui a me ka hoʻokele ʻike ke hōʻoia i ka pololei o ka ʻike.
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Kahua hoʻāʻo loiloi: ʻike ʻana i nā mea haumia organik, ʻike ʻana i nā lāʻau lapaʻau mole liʻiliʻi, ʻike ʻana i nā pathogens i lawe ʻia e ka meaʻai
Ma kēia kahua, e hoʻohana ʻia ka ʻenehana synthesis nucleic acid aptamer me ka immunochromatography gula colloidal, kahi e hoʻomākaukau mua ʻia ai ke gula colloidal a ʻike ʻia ka maikaʻi, a laila e hoʻopili ʻia i ka nucleic acid aptamer, e wehewehe ʻia a pīpī ʻia ma kahi pad nakinaki, a e hoʻomākaukau ʻia nā ʻāpana hoʻāʻo me ka hoʻohana ʻana i ka antibody polyclonal e ʻike ʻia ma ke ʻano he laina ʻike, a me ke kaʻina hoʻohui o ka nucleic acid aptamer ma ke ʻano he laina kaohi maikaʻi, i mea e kālailai ai i ke alo o ka mea i manaʻo ʻia ma ke kumu o nā hopena o nā hoʻokolohua maikaʻi ʻole a maikaʻi.
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Kahua olaola: ka hoʻomohala ʻana o nā biosensors
Hoʻohana nā mea ʻike nucleic acid aptamer i ka aptamer ma ke ʻano he mea ʻike no ka ʻike ʻana i kahi mea i manaʻo ʻia a hoʻololi i ka hopena ʻike i kahi hōʻailona hiki ke ana ʻia. Hiki ke hoʻololi ʻia ka hōʻailona ma ke ʻano electrochemical, optical, mahana, piezoelectric, magnetic a me micromechanical a i ʻole ka hui pū ʻana o kēia mau ʻano hana. Ke hoʻohālikelike ʻia me nā ʻano ʻike e kū nei, maʻalahi ka hana ʻana o nā mea ʻike nucleic acid aptamer, kikoʻī, pane a naʻauao, a ʻo kēia mau pono e maʻalahi ai ka hoʻopili ʻia ʻana i nā laʻana maoli a loaʻa nā manaʻolana maikaʻi no ka hoʻomohala ʻana.
ʻO ka lawelawe hana Aptamer SELEX
ʻO ka lawelawe synthesis aptamer SELEX a Alpha Lifetech e komo pū me ke kūkulu ʻana i ka waihona puke aptamer SELEX, ka hoʻonui ʻana iā SELEX, a me nā mea ʻē aʻe. Manaʻo ʻo Alpha Lifetech e loaʻa i nā aptamers SELEX pili kiʻekiʻe a me ke kikoʻī kiʻekiʻe no nā mea kūʻai aku ma o ke kaʻina hana nānā in vitro.
HELUHELU HOU AʻE
Ka lawelawe hoʻomohala ʻana o ka Nucleic Acid Aptamer
ʻO ka lawelawe hoʻomohala aptamer nucleic acid o Alpha Lifetech e pili nui ana i ka ʻenehana SELEX no ka nānā ʻana i ka aptamer SELEX, me ka hoʻomohala ʻana i nā aptamers DNA, nā aptamers RNA a me nā aptamers XNA, e pili ana i ka hoʻolālā waihona puke, ka synthesis oligonucleotide, ke kūkulu ʻana i ka waihona puke a me ka hōʻoia.
HELUHELU HOU AʻEInā he mau nīnau kāu, e ʻoluʻolu e hoʻokaʻaʻike mai iā mākou i kēlā me kēia manawa.
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2018-07-16 
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