Kahua Hoʻomohala Antibody Bispecific

Hiki iā Alpha Lifetech ke hāʻawi i nā mea kūʻai aku me ka ʻike ʻana i ka antibody i hōʻoia ʻia a me nā huahana a me nā lawelawe protein recombinant. Hiki iā mākou ke hoʻomākaukau i nā antibodies me ka pono kiʻekiʻe, ka kikoʻī ikaika, a me ke kūpaʻa maikaʻi. Loaʻa iā Alpha Lifetech nā ʻano mea hana a me nā lako hoʻomaʻemaʻe antibody, hiki ke hāʻawi i nā lawelawe hoʻomaʻemaʻe antibody mai nā kumu like ʻole e like me nā antibodies monoclonal rabbit, hipa, moa, a me ka ʻiole, a me nā lawelawe hoʻomaʻemaʻe Protein A/G a me nā lawelawe hoʻokaʻawale antibody a me ka hoʻomaʻemaʻe. Ma muli o ke kūkulu ʻana o ka ʻōnaehana kahua piha o ka paepae ʻike antibody, paepae protein, a pēlā aku, uhi mākou i nā lawelawe o luna a me lalo o ka hana antibody, a hiki ke hāʻawi i nā lawelawe loea mai ka hoʻomākaukau ʻana o ka antibody, ka hoʻomaʻemaʻe antibody bispecific a me ka hoʻokaʻawale antibody a me ka hoʻomaʻemaʻe, ka hoʻonohonoho ʻana o ka antibody, ka hōʻoia antibody, a pēlā aku, hiki ke hoʻohana ʻia no ka lāʻau lapaʻau antibody bispecific.

ʻO nā antibodies monoclonal e pili ana i nā immunoglobulins i hana ʻia e kahi cell B hoʻokahi i loaʻa ke kikoʻī kiʻekiʻe no nā antigens a i ʻole epitopes. Ua hoʻomākaukau mua ʻia ka hana ʻana o nā antibodies monoclonal me ka hoʻohana ʻana i ka ʻenehana hybridoma e hana i nā antibodies mouse. Ma ke ʻano kikoʻī, ma ka hoʻohui ʻana i nā cell spleen mai nā ʻiole i hoʻopili ʻia me nā cell myeloma mai nā kānaka a i ʻole nā ʻiole, ua hoʻokumu ʻia nā cell hybridoma, kahi e hūnā ai i nā antibodies kikoʻī. Hoʻohana nui ʻia nā antibodies monoclonal mouse i hana ʻia i ka noiʻi holoholona a me ka ʻike maʻi ma hope o nā lawelawe hoʻomaʻemaʻe. Eia nō naʻe, e hana ke kino kanaka i kahi pane pale i nā protein mouse haole, no laila aia nā palena koʻikoʻi i ka hoʻohana lapaʻau o ka hana ʻana o nā antibodies monoclonal mouse. Ma hope mai, ua hoʻohana ka poʻe i ka "humanization" e hoʻomākaukau i nā antibodies monoclonal mai nā ʻiole, a hoʻohana i ka ʻenehana ʻenekinia genetic e hoʻololi i nā antibodies mouse e loaʻa kahi ʻāpana mau o ka immunoglobulin kanaka e hōʻemi i ko lākou immunogenicity. Ua ʻike ʻia kēia ʻano antibody he antibody monoclonal humanized. Eia kekahi, ʻo ka hoʻohana ʻana i nā cell kanaka e hana i nā antibodies monoclonal ua kapa ʻia ʻo ka hoʻomākaukau ʻana o ka antibody monoclonal kanaka holoʻokoʻa. Hoʻohana nui ʻia nā antibodies monoclonal i nā noi biomedical a me nā noi lapaʻau. I ka noiʻi lapaʻau, hiki ke hoʻohana ʻia nā antibodies monoclonal e like me nā immunomodulators, hiki ke hoʻohana ʻia nā antibodies monoclonal therapeutic no ka chemotherapy kanesa a me ka mālama ʻana i nā maʻi viral, a ʻo ka hoʻomohala ʻana o nā monoclonal antibody conjugates e hoʻohana nui ʻia no ka mālama ʻana i ka maʻi kanesa.

Hoʻolauna i ka Antibody Bispecific

I ka makahiki 1960, ua hāpai ʻia ke kumumanaʻo o nā antibodies bispecific. ʻO nā antibodies bispecific, i ʻike ʻia hoʻi he mau antibodies monoclonal bispecific, he mau antibodies i hana ʻia me ka hoʻohana ʻana i ka ʻenehana ʻenekinia genetic. Ma ke ʻano he antibody artificial i hana ʻia, ʻo nā antibodies bispecific maʻamau no ka subclass IgG a loaʻa iā lākou kahi ʻāpana hoʻopaʻa antigen e kuhikuhi ana i ka subunit CD3. Loaʻa i nā antibodies bispecific ʻelua mau wahi hoʻopaʻa antigen kikoʻī e hiki ke hoʻopaʻa a ʻike i ʻelua antigens like ʻole, a i ʻole ʻelua epitopes like ʻole o kahi antigen. Ke hoʻohālikelike ʻia me nā antibodies monoclonal, loaʻa i nā antibodies bispecific kahi wahi hoʻopaʻa antigen kikoʻī hou aʻe, no laila loaʻa iā lākou ka specificity ikaika a me ka hiki ke kuhikuhi, hiki ke kuhikuhi pololei i nā cell tumor a hōʻemi i ka ʻawahia off target. Hiki i nā antibodies bispecific ke hana i nā hana olaola he nui i ka manawa like, e like me ka hoʻolimalima ʻana i nā cell immune, ke ālai ʻana i nā ala hōʻailona, a me ka pepehi pololei ʻana i nā cell tumor. Ua hoʻomākaukau nui ʻia nā antibodies bispecific mua ma o ka conjugation chemical a i ʻole cell fusion, akā ua holomua mālie paha kēia ʻano hana ma muli o ka hui like ʻole a me ka paʻakikī i ka hoʻokaʻawale ʻana i ka hui pahuhopu. Me ka hoʻomohala mau ʻana o ka ʻenehana ʻenekinia genetic, ua hoʻomohala ʻia nā kahua ʻenehana hou he nui, e like me nā knots in holes (KIH), CrossMab, DVD Ig, a pēlā aku. Hoʻoponopono pono kēia mau kahua i nā pilikia e like me ke kaulahao kaumaha a me nā kaulahao māmā, a hoʻomaikaʻi i ka like ʻana a me ka hua o nā antibodies bispecific.

Kiʻi 1. ʻIke maʻalahi o nā ʻano hana i manaʻo ʻia no nā antibodies bispecific (bsAbs) i nā hoʻokolohua lapaʻau no ka oncology.(Kumu kiʻi: He loiloi o nā antibodies bispecific a me nā mea hana antibody i ka oncology a me nā pilikia lapaʻau - ScienceDirect)

ʻenehana hana antibodies bispecific

ʻO nā ʻano hana nui no ka hana ʻana i ka antibody bispecific e komo pū me ka hoʻopili kemika, ka hybridoma kumu ʻehā, a me ka hoʻomākaukau ʻana i ka antibody ʻenekinia genetic. I waena o lākou, ʻo ke ʻano hoʻopili kemika e hoʻopili i ʻelua IgG paʻa a i ʻole ʻelua mau ʻāpana antibody F (ab ') 2 i loko o nā antibodies bispecific me ka hoʻohana ʻana i nā mea hoʻopili kemika e like me phthalimide a me ka dithioacylbenzoic acid. He maʻalahi a maʻalahi hoʻi kēia ʻano hana e hana, akā hiki ke hōʻino i ka wahi hoʻopaʻa antigen, hoʻemi i ka hana antibody, a loaʻa i ka mea hoʻopili ponoʻī kekahi kekelē o ka carcinogenicity. Hoʻokumu ʻia ke ʻano hybridoma kumu ʻehā ma ka hoʻohui ʻana o nā cell somatic mai ʻelua mau laina cell hybridoma like ʻole e hōʻike i ka IgG mouse like. Ma o ka ʻenehana ʻenekinia genetic, hiki ke hoʻololi ʻia nā antibodies e hana i nā antibodies bispecific. Ua kūkulu ʻia ʻelua mau antibodies monoclonal like ʻole, a ua ʻoki ʻokoʻa ʻia nā ʻāpana Fab a i ʻole nā ʻāpana loli kaulahao kaumaha a me nā kaulahao māmā o nā antibodies ʻelua. Ma o ka hana cross-linking a i ʻole ka ʻenehana recombination kaulahao, ua hui pū ʻia nā ʻāpana ʻelua e hana i kahi antibody bispecific. ʻOiai ke kiʻekiʻe o ka paʻakikī o ka ʻenehana ʻenekinia genetic, ʻo ia ke ʻano i hoʻohana pinepine ʻia no ka hana ʻana i ka antibody bispecific e hoʻoponopono i ke ʻano a me ka hana o nā antibodies. I ka wā e hana ai i ka hoʻolālā antibody bispecific, hiki ke hoʻohana ʻia ke kumumanaʻo o ka antibody cross reactivity, akā hiki i ka antibody cross reactivity ke hana i nā hopena non-specific, no laila e noʻonoʻo pono ʻia i ka hoʻohana pono ʻana o ka hoʻolālā antibody bispecific, e like me ka bispecific antibody therapy.

Hoʻomaʻemaʻe ʻana i nā Antibodies Bispecific

ʻO ka hoʻomaʻemaʻe ʻana i ka antibody bispecific ke kaʻina hana o ka hoʻokaʻawale ʻana a me ka hoʻomaʻemaʻe ʻana i nā antibodies target kiʻekiʻe-maʻemaʻe. ʻElua mau ʻano hana, ʻo ka centrifugation a me ka kānana hohonu, e hoʻohana ʻia e wehe i kekahi mau haumia hiki ke hoʻoheheʻe ʻia. Hoʻopaʻa mua ʻia ka antibody bispecific target e ka affinity chromatography. No nā antibodies bispecific e like me IgG, hoʻohana ʻia ka protein A affinity chromatography, ʻoiai no nā antibodies bispecific ʻaʻole like me IgG, hiki ke hoʻohana ʻia ka light chain affinity chromatography. Ma hope iho, hoʻomoʻa ʻia ka antibody ma lalo o nā kūlana pH haʻahaʻa no kekahi manawa, e hoʻopilikia ana i ke ʻano o ka protein ma luna o ka envelope virus, a laila e nalowale ana ka hiki ke hoʻopili i nā cell. Hana ʻia ka kānana hohonu waena e wehe hou aku i nā haumia e like me nā protein cell host (HCP). Hoʻomaikaʻi ʻia ka maʻemaʻe o nā antibodies ma o nā ʻano hana e like me ka ion exchange chromatography, a wehe ʻia nā virus i koe ma o nā ʻenehana nanofiltration a i ʻole ultrafiltration. ʻO ka hope, ua hoʻopaʻa ʻia ka laʻana a pani ʻia me kahi buffer formulation kūpono.

Kaʻina Hana Hana Hana Antibodies Bispecific

Nā ʻanuʻu	ʻIke lawelawe	Ka Papa Manawa
Hana ʻana o nā Gene	Ka hana ʻana a hiki i 3 mau hoʻolālā hoʻolālā like ʻole o nā kaʻina, ka hana de novo o nā plasmids DNA antibody recombinant	2-3 pule
Hoʻāʻo liʻiliʻi	Ka hana ʻana o nā protein antibody bispecific → Hōʻike liʻiliʻi ma nā laina cell mammalian → Hōʻoia e SDS-PAGE → Ka nānā ʻana me nā antigens protein recombinant e ELISA → Hoʻohālikelike o nā clones me nā antibodies makua	5-6 mau pule
E ʻike	Hōʻike ʻana o nā antibodies bispecific piha piha ʻelua → Ka nānā ʻana o ka hoʻopaʻa ʻana e ELISA → Ka loiloi ELISA o nā antibodies i ʻike ʻia e nā antibodies anti mouse	1 pule
Hana ʻana i nā antibodies	Ka hana ʻana o ka antibody nui	3-4 pule

Ko mākou mau pono

Uhi mākou i nā lawelawe ma luna a ma lalo o ka hana ʻana o ka antibody, a hiki ke hāʻawi i nā lawelawe loea mai ka hoʻomākaukau ʻana o ka antibody, ka hoʻomaʻemaʻe ʻana o ka antibody bispecific a me ka hoʻokaʻawale ʻana a me ka hoʻomaʻemaʻe ʻana o ka antibody, ka hoʻonohonoho ʻana o ka antibody, ka hōʻoia ʻana o ka antibody, a pēlā aku, hiki ke hoʻohana ʻia no ka lāʻau lapaʻau antibody bispecific.

Hōʻike Kiʻekiʻe

Ka hōʻike ʻana o ka protein kiʻekiʻe i loko o nā pūnaewele CHO.
Ka Waihona Paʻa

>2 mau pule i loko o ke koko ma 37 °C.
ʻAʻohe pilikia hoʻoheheʻe ʻia

>30 mg/ml
Nā Lnes Kelepona Paʻa

Ua hoʻomohala ʻia nā laina cell paʻa he nui me nā antigens tumor e hōʻoia i nā antibodies bispecific he nui