Hōʻike ʻia ʻo Phage Antibody Development Platform

ʻO Alpha Lifetech, me kāna ʻike papahana nui i ka ʻike ʻana i ka antibody, hiki ke hāʻawi i nā lawelawe antibody monoclonal a polyclonal maʻamau no nā ʻano he nui, a me ka hale waihona puke antibody a me nā lawelawe screening. Hiki iā Alpha Lifetech ke hāʻawi aku i nā mea kūʻai aku me ka maikaʻi i hōʻoia ʻia i ka antibody a me nā huahana antibody recombinant, a me nā lawelawe kūpono, e hoʻomākaukau ai i nā antibodies kūpono, kikoʻī, a paʻa. Ma ka hoʻohana ʻana i nā paepae antibody piha a me ka ʻenehana antibody, hāʻawi mākou i nā lawelawe e uhi ana i ka upstream a me lalo o ka hana antibody, me nā lawelawe ʻenehana e like me ka hoʻomākaukau ʻana i ka antibody, hoʻomaʻemaʻe, antibody sequencing, a me ka hōʻoia.

Loaʻa iā Alpha Lifetech kahi kahua ʻike antibody makua e hāʻawi ana i nā mea kūʻai aku me ka nui o nā lawelawe e pili ana i ka palekana holoholona, hōʻike phage hale waihona puke antibody a me ka screening, antibody sequencing, antibody expression, antibody hoʻomaʻemaʻe, antibody validation, a me ka hōʻailona antibody e pili ana i ka ʻenehana hybridoma, ka ʻenehana cell B hoʻokahi, ka ʻenehana hōʻike phage, a me nā mea hou aku. He laina hana maʻamau ka Alpha Lifetech, a ma waho aʻe o nā lawelawe hoʻomākaukau antibody, hiki ke hāʻawi ʻia nā lawelawe kākoʻo e like me ka antibody humanization, antibody affinity maturation, ADC lāʻau hoʻolālā a me ka hoʻomohala ʻana, a me CAR-T hiki ke hāʻawi ʻia i ka hoʻolālā sequence. Ma ka manawa like, ua kūkulu ʻo Alpha Lifetech i kahi waihona antibody e pili ana i ka M13, T4, a me T7 phage hōʻike ʻenehana platform, me ka hiki ke mālama ʻia a hiki i 10 ^ 8-10 ^ 9. ʻO ka helu kūpono, ka helu hoʻokomo, a me ka ʻokoʻa o ka hale waihona puke hiki ke piʻi ma luna o 90%.

Hōʻike ʻenehana Phage

Ua hoʻomākaukau mua ʻia ka hana ʻana o nā antibodies monoclonal me ka hoʻohana ʻana i ka ʻenehana antibody hybridoma monoclonal antibody e hana i ka ʻiole hybridoma monoclonal antibody. Ma ke ʻano kikoʻī, ma ka hoʻohui ʻana i nā pūnaewele spleen mai nā ʻiole i hoʻopaʻa ʻia me nā ʻiole myeloma mai ke kanaka a i ʻole ka ʻiole, ua hoʻokumu ʻia nā cell hybridoma, kahi e huna ai i nā antibodies monoclonal antibody hybridoma kūikawā. Ma o ka hoʻololi kanaka ʻana o nā antibodies monoclonal ʻiole a me ka hoʻololi ʻana i ka genetic engineering o nā antibodies e hāʻawi iā lākou i kahi ʻāpana immunoglobulin kanaka mau, hiki ke hoʻemi ʻia ko lākou immunogenicity. Hoʻohana nui ʻia ka ʻenehana antibody monoclonal i nā noi biomedical a me nā lāʻau lapaʻau.

Hiki i ka Alpha Lifetech ke hāʻawi i nā lawelawe ʻenekinia antibody hoʻokahi mai ka hoʻolālā protocol noiʻi preclinical i ka ʻike ʻana i nā antibody drug conjugates (ADCs) a me ka hōʻoia holoholona. Hiki i nā antibody drug conjugates (ADCs) ke hāʻawi i nā lāʻau lapaʻau chemotherapy i nā maʻi kanesa. Ma hope o ka hoʻopaʻa ʻana i nā pahuhopu kikoʻī i hōʻike ʻia ma nā pūnaewele maʻi maʻi, hoʻokuʻu ʻo ADC i nā lāʻau cytotoxic i loko o nā maʻi kanesa. Hiki iā Alpha Lifetech ke hāʻawi aku i nā mea kūʻai aku me nā lawelawe hoʻomaʻamaʻa antibody affinity maturation. Me ka holomua mutation optimization a me ka high-throughput phage display screening technology, antibodies me kekahi affinity e nānā mua ʻia, a laila hoʻokomo ʻia nā mutations amino acid e hana i nā ʻano like ʻole. A laila, loiloi ʻia nā antibodies pili kiʻekiʻe a nānā ʻia me ka hoʻohana ʻana i nā ʻenehana screening. Ma hope o nā pōʻai he nui o ka optimization a me ka nānā ʻana i ke kūkulu ʻana, ua loaʻa ka antibodies me ka pilina kiʻekiʻe a me ka kikoʻī ikaika.

Hiki i ka Alpha Lifetech ke hana i nā ʻano like ʻole o nā hale waihona puke hōʻike antibody phage, me nā hale waihona immune, nā hale waihona puke maoli, nā hale waihona semi synthetic, a me nā hale waihona synthetic. Ma muli o ka nui o nā hale waihona puke antibody, hiki ke loaʻa nā antibodies kikoʻī loa. Hiki ke hāʻawi ʻia i nā vectors phagemid lehulehu e like me pMECS, pComb3X, a me pCANTAB 5E, a me nā kānana e like me TG1 E. coli, XL1 Blue, a me ER2738. Ma waho aʻe o ka mana waihona a hiki i ka 10^9, ʻoi aku ka kiʻekiʻe o ka helu o ka hoʻokomo ʻana i nā ʻāpana ʻāpana o ka waihona, e hana ana i nā kūlana kūpono no ka nānā ʻana i nā antibodies e māʻona ai nā mea kūʻai. Hōʻike ʻia ka papa kuhikuhi o ka lawelawe hoʻomākaukau antibody a Alpha Lifetech e pili ana i ka ʻenehana hōʻike phage ma ke Kiʻi 1.

Fig.1 Ka papa hana no ka hoʻomākaukau ʻana i nā antibodies monoclonal ma muli o ka ʻenehana hōʻike phage.

Hōʻike ʻo Phage Antibody Production Workflow

ʻanuʻu	Mea lawelawe	Laina manawa
KaʻAnuʻu 1: Kaʻi holoholona	(1) Hoʻomaʻamaʻa holoholona 4 manawa, booster immunization 1 dose, a pau 5 doses i ka maʻi. (2) Negative serum ma mua o ka hōʻiliʻili ʻia ʻana o ka lāʻau lapaʻau, a ua hana ʻia ʻo ELISA ma ka hā o ka nui e ʻike ai i ka titer serum. (3) Inā hoʻokō ka serum antibody titer o ka ʻehā i nā koi, e hāʻawi ʻia hoʻokahi maʻi o ka lāʻau lapaʻau i 7 mau lā ma mua o ka hōʻiliʻili koko. Inā ʻaʻole ia e hoʻokō i nā koi, e hoʻomau ʻia ka hoʻomaʻamaʻa maʻamau. (4) Ka mana kūpono, ka hōʻiliʻili koko a me ka hoʻokaʻawale ʻana o nā monocytes	10 pule
KaʻAnuʻu 2: hoʻomākaukau cDNA	(1) PBMC Total RNA Extraction (RNA Extraction Kit) (2) Hoʻomākaukau RT-PCR kūpaʻa kiʻekiʻe o ka cDNA (ke kiʻi transcription reverse)	1 lā
KaʻAnuʻu Hana 3: kūkulu i ka Antibody Library	(1) Ke hoʻohana nei i ka cDNA ma ke ʻano he laʻana, ua hoʻonui ʻia nā genes e nā pōʻai ʻelua o PCR. (2) Ka hana ʻana a me ka hoʻololi ʻana: gene splicing phagemid vector, electroporation transformation o TG1 host bacteria, ke kūkulu ʻana i ka waihona antibody. (3) Identification: E koho wale i 24 clones, PCR identification positive rate+insert rate. (4) Hoʻomākaukau phage kōkua: M13 phage amplification + hoʻomaʻemaʻe. (5) Hoʻopakele hale waihona hōʻike Phage	3-4 pule
KaʻAnuʻu Hana 4: Nānā Hale Waihona Antibody (3 puni)	(1) Ka nānā 'ana i ka 3-puni paʻamau (ka nānā ʻana i ka pae paʻa): ka nānā ʻana i ke kaomi no ka wehe ʻana i nā antibodies kikoʻī ʻole i ka nui loa. (2) Hoʻokahi clone amplification bacteriophage i koho ʻia + IPTG i hoʻokomo ʻia i ka ʻōlelo + ELISA ʻike o nā clones maikaʻi. (3) Ua koho ʻia nā clones maikaʻi a pau no ke kaʻina hana gene.	4-5 pule

Pōmaikaʻi o phage Hōʻike Technology

Loaʻa ka ʻike waiwai ʻo Alpha Lifetech i ke kahua o ka hoʻomohala antibody. I nā makahiki, ua hoʻokumu ʻo Alpha Lifetech i kahi kahua hoʻomohala antibody.

Nā lawelawe kākoʻo

Hiki iā mākou ke hāʻawi i nā lawelawe kūkulu hale waihona immune e pili ana i nā holoholona a me nā lawelawe nānā ʻana o ka hale waihona antibody kūlohelohe e like me ka makemake o ka mea kūʻai

Pahu Lahui

Loaʻa nā lawelawe ʻike antibody target he nui: nā protein, peptides, nā mole liʻiliʻi, nā maʻi, nā protein membrane, mRNA, etc.

Nā Vectors Nui

ʻO ka lawelawe hale waihona puke pilikino, hiki iā mākou ke hāʻawi i nā vectors bacteriophage like ʻole me PMECS, pComb3X, a me pCANTAB 5E, a hoʻololi iā lākou e like me nā pono o ka mea kūʻai aku.

Papahana makua

Hiki i ka hiki ke mālama i ka 10 ^ 8-10 ^ 9, ʻoi aku ka nui o ka hoʻokomo ʻana ma luna o 90%, a ʻo ka pilina o nā antibodies i loaʻa ma o ka nānā ʻana aia ma ka pae nM pM.

HANA pili

Nā Hoʻolālā Hoʻolālā Antibody Nui

ʻOihana Hoʻomohala ʻAi ʻAi Monoclonal

Papahana ʻenehana Hybridoma

Hoʻokahi B Pūnaewele Wehewehe Papahana

Hōʻike ʻia ʻo Phage Antibody Development Platform

hoʻomohala ʻana i ka antibody monoclonal-Alpha Lifetech

ʻOihana Hoʻomohala ʻAi ʻAi Monoclonal

Hiki iā mākou ke hāʻawi i nā lawelawe hoʻomohala monoclonal antibody kiʻekiʻe kiʻekiʻe, kiʻekiʻe-maʻemaʻe, a me ka hana ʻana i nā antibodies monoclonal kiʻi a me nā antibodies monoclonal rabbit.

Papahana ʻenehana Hybridoma

Hoʻopili pū ʻia me ka papahana o ka pale ʻana, nā lawelawe hoʻomākaukau antibody, hoʻomaʻemaʻe antibody, antibody high throughput sequencing, antibody validation, etc.

b ka nānā ʻana i ke kelepona-Alpha Lifetech

Hoʻokahi B Pūnaewele Wehewehe Papahana

Loaʻa iā Alpha Lifetech nā pono i ka manawa screening a me ka loaʻa ʻana o nā antibodies kiʻekiʻe. Hiki iā ia ke hāʻawi i ka hoʻolālā antigen, synthesis, a me ka hoʻololi ʻana, ka pale ʻana i nā holoholona, ka nānā ʻana i ka hoʻonui ʻana i ka cell B hoʻokahi, ka hoʻonohonoho ʻana o ka cell cell.