Kahua Hoʻomohala Antibody Hōʻike Phage

ʻO Alpha Lifetech, me kona ʻike nui i ka papahana i ka ʻike ʻana i ka antibody, hiki iā ia ke hāʻawi i nā lawelawe antibody monoclonal a me polyclonal i hana kūikawā ʻia no nā ʻano he nui, a me ke kūkulu ʻana i ka waihona puke antibody a me nā lawelawe nānā. Hiki iā Alpha Lifetech ke hāʻawi i nā mea kūʻai aku me nā huahana antibody i hōʻoia ʻia a me nā huahana antibody recombinant, a me nā lawelawe pili, e hoʻomākaukau i nā antibodies kūpono, kikoʻī loa, a paʻa. Ma ka hoʻohana ʻana i nā kahua antibody piha a me ka ʻenekinia antibody, hāʻawi mākou i nā lawelawe e uhi ana i ka ʻaoʻao mua a me ka hope o ka hana antibody, me nā lawelawe loea e like me ka hoʻomākaukau ʻana o ka antibody, ka hoʻomaʻemaʻe ʻana, ka hoʻonohonoho ʻana o ka antibody, a me ka hōʻoia.

Loaʻa iā Alpha Lifetech kahi kahua ʻike antibody makua e hāʻawi ana i nā mea kūʻai aku me nā ʻano lawelawe like ʻole me ka pale ʻana o nā holoholona, ke kūkulu ʻana a me ka nānā ʻana i ka waihona puke antibody hōʻike phage, ka hoʻonohonoho ʻana o ka antibody, ka hōʻike ʻana o ka antibody, ka hoʻomaʻemaʻe ʻana o ka antibody, ka hōʻoia ʻana o ka antibody, a me ka lepili ʻana i ka antibody e pili ana i ka ʻenehana hybridoma, ka ʻenehana cell B hoʻokahi, ka ʻenehana hōʻike phage, a me nā mea hou aku. Loaʻa iā Alpha Lifetech kahi laina hana maʻamau, a ma waho aʻe o nā lawelawe hoʻomākaukau antibody, hiki ke hāʻawi ʻia nā lawelawe kākoʻo e like me ka antibody humanization, antibody affinity maturation, ADC drug design and development, a me ka hoʻolālā ʻana o ka CAR-T ma hope. I ka manawa like, ua kūkulu ʻo Alpha Lifetech i kahi waihona puke antibody e pili ana i nā kahua ʻenehana hōʻike phage M13, T4, a me T7, me ka hiki ke mālama a hiki i ka 10^8-10^9. Hiki i ka helu maikaʻi, ka helu hoʻokomo, a me ke ʻano like ʻole o ka waihona puke ke hiki aku ma mua o 90%.

ʻenehana hōʻike Phage

Ua hoʻomākaukau mua ʻia ka hana ʻana o nā antibodies monoclonal me ka hoʻohana ʻana i ka ʻenehana antibody monoclonal hybridoma e hana i ka antibody monoclonal hybridoma mouse. ʻO ke kikoʻī, ma ka hoʻohui ʻana i nā cell spleen mai nā ʻiole i hoʻopili ʻia me nā cell myeloma mai nā kānaka a i ʻole nā ʻiole, ua hoʻokumu ʻia nā cell hybridoma, kahi e hūnā ai i nā antibodies antibody monoclonal hybridoma kikoʻī. Ma ka hoʻololi kanaka ʻana o nā antibodies monoclonal mouse a me ka hoʻololi ʻenekinia genetic o nā antibodies e hāʻawi iā lākou i kahi ʻāpana immunoglobulin kanaka mau, hiki ke hoʻemi ʻia ko lākou immunogenicity. Hoʻohana nui ʻia ka ʻenekinia antibody monoclonal i nā noi biomedical a me nā noi lapaʻau.

Hiki iā Alpha Lifetech ke hāʻawi i nā lawelawe ʻenekinia antibody hoʻokahi-kū mai ka hoʻolālā protocol noiʻi preclinical a hiki i ka ʻike ʻana i nā antibody drug conjugates (ADCs) a me ka hōʻoia holoholona. Hiki i nā antibody drug conjugates (ADCs) ke hāʻawi i nā lāʻau chemotherapy i nā cell cancer. Ma hope o ka hoʻopaʻa ʻana i nā pahuhopu kikoʻī i hōʻike ʻia ma nā cell cancer, hoʻokuʻu ʻo ADC i nā lāʻau cytotoxic i loko o nā cell cancer. Hiki iā Alpha Lifetech ke hāʻawi i nā mea kūʻai aku me nā lawelawe hoʻomaʻamaʻa antibody affinity piha. Me ka hoʻonui ʻana i ka mutation holomua a me ka ʻenehana nānā ʻana i ka hōʻike phage kiʻekiʻe, ua nānā mua ʻia nā antibodies me kekahi affinity, a laila hoʻolauna ʻia nā mutations amino acid e hana i nā ʻano like ʻole. Ma hope mai, ua loiloi ʻia a nānā ʻia nā antibodies affinity kiʻekiʻe me ka hoʻohana ʻana i nā ʻenehana nānā. Ma hope o nā pōʻai he nui o ka hoʻonui ʻana a me ka nānā ʻana i ke ʻano, ua loaʻa hope nā antibodies me ka affinity kiʻekiʻe a me ka specificity ikaika.

Hiki iā Alpha Lifetech ke kūkulu i nā ʻano waihona hōʻikeʻike antibody phage like ʻole, me nā waihona pale, nā waihona kūloko, nā waihona semi synthetic, a me nā waihona synthetic. Ma muli o ka hiki ke kiʻekiʻe o nā waihona antibody, hiki ke loaʻa nā antibodies kikoʻī loa. Hiki ke hāʻawi ʻia nā vectors phagemid he nui e like me pMECS, pComb3X, a me pCANTAB 5E, a me nā ʻano e like me TG1 E. coli, XL1 Blue, a me ER2738. Ma waho aʻe o ka hiki ke waihona a hiki i ka 10^9, kiʻekiʻe nō hoʻi ka helu hoʻokomo ʻāpana target o ka waihona, e hana ana i nā kūlana lawa no ka nānā ʻana i nā antibodies e hoʻomāʻona i nā mea kūʻai aku. Hōʻike ʻia ka flowchart o ka lawelawe hoʻomākaukau antibody a Alpha Lifetech e pili ana i ka ʻenehana hōʻikeʻike phage ma ke Kiʻi 1.

Kiʻi 1 Kiʻikuhi hana no ka hoʻomākaukau ʻana i nā antibodies monoclonal e pili ana i ka ʻenehana hōʻike phage.

Kaʻina Hana Hana Antibody Hōʻike Phage

Nā ʻanuʻu	ʻIke lawelawe	Ka Papa Manawa
KaʻAnuʻu Hana 1: Ka pale ʻana i nā holoholona	(1) Hoʻopili ʻia nā holoholona i 4 mau manawa, hoʻopili ʻia nā holoholona i 1 mahele lāʻau, he 5 mau mahele lāʻau i hoʻopili ʻia. (2) ʻAʻole maikaʻi ka serum ma mua o ka hōʻiliʻili ʻia ʻana o ka immunization, a ua hana ʻia ʻo ELISA ma ka hā o ka mahele e ʻike ai i ka serum titer. (3) Inā kūlike ka titer antibody serum o ka hā o ka mahele i nā koi, e lawelawe ʻia hoʻokahi mahele hou o ka immunization i 7 mau lā ma mua o ka hōʻiliʻili koko. Inā ʻaʻole ia e kūlike i nā koi, e hoʻomau ʻia ka immunization maʻamau. (4) Ka ikaika kūpono, ka hōʻiliʻili koko a me ka hoʻokaʻawale ʻana o nā monocytes	10 pule
KaʻAnuʻu Hana 2: Hoʻomākaukau ʻana o ka cDNA	(1) PBMC Huina RNA Unuhi (RNA Extraction Kit) (2) Hoʻomākaukau RT-PCR kiʻekiʻe o ka cDNA (reverse transcription kit)	1 lā
KaʻAnuʻu Hana 3: Ke Kūkulu ʻana o ka Hale Waihona Puke Antibody	(1) Ma ka hoʻohana ʻana i ka cDNA ma ke ʻano he template, ua hoʻonui ʻia nā genes e ʻelua mau puni o PCR. (2) Ke kūkulu ʻana a me ka hoʻololi ʻana o ka phage: ka hoʻopili ʻana o ka gene phagemid vector, ka hoʻololi ʻana o ka electroporation o ka bacteria host TG1, ke kūkulu ʻana o ka waihona puke antibody. (3) Ka ʻike ʻana: E koho maʻamau i 24 mau clones, ka helu ʻike PCR maikaʻi + ka helu hoʻokomo. (4) Kōkua i ka hoʻomākaukau ʻana o ka phage: hoʻonui ʻana i ka phage M13 + hoʻomaʻemaʻe. (5) Hoʻopakele hale waihona puke hōʻikeʻike Phage	3-4 pule
KaʻAnuʻu Hana 4: Nānā ʻana i ka Hale Waihona Puke Antibody (3 mau puni)	(1) Nānā ʻana o nā puni 3 maʻamau (nānā pae paʻa): nānā ʻana i ke kaomi e wehe i nā antibodies kikoʻī ʻole i ka nui loa. (2) Hoʻonui ʻia ka bacteriophage clone hoʻokahi i koho ʻia + hōʻike i hoʻokomo ʻia e IPTG + ʻike ʻana o ELISA i nā clones maikaʻi. (3) Ua koho ʻia nā clones maikaʻi a pau no ke kaʻina hana gene.	4-5 mau pule

Ka Pōmaikaʻi o ka ʻenehana hōʻike phage

He nui ka ʻike o Alpha Lifetech ma ke kahua o ka hoʻomohala ʻana i nā antibody. I nā makahiki i hala iho nei, ua hoʻokumu ʻo Alpha Lifetech i kahi kahua hoʻomohala antibody piha.

Nā lawelawe kākoʻo

Hiki iā mākou ke hāʻawi i nā lawelawe kūkulu hale waihona puke pale holoholona like ʻole a me nā lawelawe nānā hale waihona puke antibody kūlohelohe e like me nā pono o ka mea kūʻai aku

Pahuhopu he nui

Loaʻa nā lawelawe ʻike antibody pahuhopu he nui: nā protein, nā peptides, nā mole liʻiliʻi, nā virus, nā protein membrane, mRNA, a pēlā aku.

Nā Vectors he nui

ʻO ka lawelawe kūkulu hale waihona puke pilikino, hiki iā mākou ke hāʻawi i nā vectors bacteriophage like ʻole e like me PMECS, pComb3X, a me pCANTAB 5E, a hoʻololi iā lākou e like me nā pono o ka mea kūʻai aku

Paepae makua

Hiki i ka hiki ke mālama i ka 10 ^ 8-10 ^ 9, ʻoi aku ka nui o nā helu hoʻokomo ma mua o 90%, a ʻo ka pilina o nā antibodies i loaʻa ma o ka nānā ʻana ma ka pae nM pM.

LAWELAWE Pili

Nā Hoʻolālā Hoʻomohala Antibody He Nui

Ka lawelawe hoʻomohala ʻana i ka lāʻau lapaʻau Monoclonal

Kahua ʻenehana Hybridoma

Kahua Hoʻokaʻawale Kelepona B Hoʻokahi

Kahua Hoʻomohala Antibody Hōʻike Phage

ka hoʻomohala ʻana i ka antibody monoclonal-Alpha Lifetech

Ka lawelawe hoʻomohala ʻana i ka lāʻau lapaʻau Monoclonal

Hiki iā mākou ke hāʻawi i nā lawelawe hoʻomohala antibody monoclonal kiʻekiʻe, maʻemaʻe kiʻekiʻe, a me ke kikoʻī loa, me ka hana ʻana o nā antibodies monoclonal mouse a me nā antibodies monoclonal rabbit.

Kahua ʻenehana Hybridoma

Me ka papahana immunization, nā lawelawe hoʻomākaukau antibody, ka hoʻomaʻemaʻe antibody, ka antibody high throughput sequencing, ka antibody validation, a pēlā aku

Kahua Hoʻokaʻawale Kelepona B Hoʻokahi

Loaʻa iā Alpha Lifetech nā pono i ka manawa nānā a me ka loaʻa ʻana o nā antibodies kiʻekiʻe. Hiki iā ia ke hāʻawi i ka hoʻolālā antigen, synthesis, a me ka hoʻololi ʻana, ka palekana holoholona, ka nānā ʻana i ka hoʻonui ʻana o nā cell B hoʻokahi, ka hoʻonohonoho ʻana o nā cell hoʻokahi.