Kahua Kūkulu Hale Waihona Puke Peptide Hōʻike Phage

He nui nā ʻenehana kūkulu hale waihona puke peptide a Alpha Lifetech. Hāʻawi nui mākou i ka lawelawe kūkulu hale waihona puke peptide T7 a me ka lawelawe kūkulu hale waihona puke peptide M13 e like me nā ʻano phage; hāʻawi mākou i ka lawelawe kūkulu hale waihona puke peptide loop 7 linear a me ka lawelawe kūkulu hale waihona puke peptide 12 e like me ka nui o ke kaʻina o nā mole peptide, a pēlā aku. Hāʻawi pū mākou i ka lawelawe kūkulu hale waihona puke peptide i maʻamau ʻia. Hāʻawi pū mākou i nā lawelawe synthesis peptide a me ke kūkulu ʻana i maʻamau ʻia.

ʻO ka ʻenehana hōʻike Phage ma ke ʻano he ʻenehana no ka panning ʻana i nā peptides exogenous hana i loko o ka phage i ka ʻili o ka capsid, ʻo ka mālama ʻana i ka hana kūkulu a me ka hana o ka protein exogenous a i ʻole peptide ʻaʻole ia e hoʻopilikia i ka hiki o ka phage recombinant e hoʻopili i ka cell host. Hiki i kēlā me kēia ʻāpana viral phage ke hōʻike i hoʻokahi peptide random exogenous ma kona ʻili, a ʻo ka hōʻiliʻili ʻana o nā phages āpau e hōʻike ana i nā peptides like ʻole e hana ana i kahi waihona peptide hōʻike phage. He mau mea hana ikaika nā waihona peptide hōʻike phage no ka hōʻike ʻana i nā peptides synthesized maʻamau he nui a me ka nānā ʻana o ka peptide kikoʻī, a ua hoʻohana nui ʻia ma nā kahua o ka nānā ʻana o ka epitope antigenic, ka nānā ʻana i nā lāʻau lapaʻau, a me ka hoʻomohala ʻana i ka lāʻau lapaʻau.

Hoʻolauna i ke kūkulu ʻana o ka hale waihona puke Peptide

ʻO ke kūkulu ʻana o ka waihona puke peptide ka hoʻonui ʻana i nā waihona puke peptide ma o ka hoʻopili ʻana i nā moʻo DNA peptide i nā ʻano like ʻole i nā moʻo protein pūpū phage like, a me ka hoʻohana ʻana i ka hoʻākoakoa hou ʻana o phage e hoʻolei i ka peptide i manaʻo ʻia ma luna o ka ʻili phage. ʻO ke kūkulu ʻana o ka waihona puke peptide e pili ana i ka ʻenehana hōʻike phage no ka māhele biosynthesis o ka peptide synthesis, a aia nā ʻano hana peptide synthesis wai-phase a me nā ʻano hana peptide synthesis paʻa-phase ma ka mākeke. I kēia manawa, loaʻa iā Alpha Lifetech nā ʻano ʻenehana kūkulu waihona puke peptide like ʻole, hiki ke hāʻawi i nā waihona puke peptide i hana mua ʻia a me nā waihona puke peptide randomized e like me ka nui o ke kaʻina o nā mole peptide, me ke kūkulu ʻana o ka waihona puke 7-peptide, ke kūkulu ʻana o ka waihona puke 12-peptide, ke kūkulu ʻana o ka waihona puke 15-peptide, ke kūkulu ʻana o ka waihona puke 7-peptide cyclic, a me ke kūkulu ʻana o ka waihona puke 12-peptide cyclic, a hiki ke hoʻopilikino ʻia e synthesize i nā waihona puke peptide no nā ʻano like ʻole a me nā noi.

Ka Hoʻohana ʻana i ke Kūkulu ʻana a me ka Nānā ʻana o ka Waihona Puke Peptide

ʻO nā hale waihona puke peptide randomized e ka ʻenehana hōʻike phage e hiki ai ke komo i nā peptides a hāʻawi i kahi loulou kino ma waena o ka phenotype (peptide i hōʻike ʻia) a me ka genotype (DNA i hoʻopili ʻia); kūpono kēia mau hale waihona puke no nā kaʻina hana screening e hoʻokaʻawale i nā clones i hoʻopaʻa ʻia mai nā clones i hoʻopaʻa ʻole ʻia e ka hoʻomaʻemaʻe affinity. Hiki ke hoʻohana ʻia nā hale waihona puke peptide i hōʻike ʻia e Phage no (i) ke ʻano o nā epitopes cellular immune (ii) ka nānā ʻana o nā peptides bioactive receptor- a i ʻole protein-bound, nā peptides i hoʻopaʻa ʻia i nā pahuhopu non-protein, nā peptides cell- a i ʻole organ-specific, etc. a me (iii) ka hoʻomohala ʻana i nā ʻōnaehana lawe lāʻau lapaʻau peptide-mediated.

Kaʻina Hana Waihona Puke Peptide Hōʻike Phage

Kiʻi 1 Kahua Hana Hale Waihona Puke Peptide Hōʻikeʻike Phage

Kaʻina Hana Kūkulu Hale Waihona Puke Peptide Hōʻike Phage

Kaʻina Hana	ʻIke lawelawe	Ka Papa Manawa
1. Hana ʻana i ka moʻo Nucleotide	Hoʻohuihui maʻamau o nā moʻo nucleotide o ka lōʻihi i kuhikuhi ʻia e ka mea kūʻai aku	2-3 pule
2. Polymerization a me ka Digestion	ʻO ka polymerization a me ka digestion o ka synthesized DNA sequence	1 lā
3. Ke kūkulu ʻana i nā hale waihona puke electroconversion	Ua kūkulu ʻia nā bacteria kikowaena ER2738, a laila ua hoʻololi ʻia ʻo E. coli me ka plasmid recombinant i kūkulu maikaʻi ʻia.	1 pule
4. Ka nui o ka waihona puke Peptide a me ka ʻike ʻana i nā ʻano like ʻole	Ka ʻike ʻana i ka nui o ka waihona puke peptide ma o ka helu ʻana o ke kolone a me ka ʻokoʻa o ka waihona puke peptide ma o ka sequencing	2-3 lā
5. Ke kūkulu ʻana i ka hale waihona puke Peptide Phage Display	Ma ka hoʻopili ʻana i ka waihona peptide phage packaging	1 pule