Paepae Waihona Puke Peptide Hōʻike Phage

Ma muli o ka ʻenehana hōʻike phage, hiki iā Alpha Lifetech ke hāʻawi i nā mea kūʻai aku me ke kūkulu ʻana i ka hale waihona puke peptide hōʻike phage kiʻekiʻe a me nā lawelawe nānā ʻana me nā hale waihona puke linear 7 peptide, nā hale waihona puke linear 9 peptide, nā hale waihona puke linear 10 peptide, nā hale waihona puke linear 15 peptide, nā hale waihona puke linear 12 peptide, nā hale waihona puke cyclic 7 peptide, a me nā hale waihona puke peptide ʻē aʻe, ka ʻokoʻa o ka hale waihona puke, ka helu hoʻokomo, hiki i ka helu maikaʻi ke piʻi ma mua o 90%, e hoʻokō i nā pono like ʻole o nā mea kūʻai aku like ʻole no nā hale waihona puke peptide hōʻike phage.

Hoʻohana nui ʻia nā hale waihona hōʻikeʻike phage i ka noiʻi biomedical a me nā noi. No ka laʻana, i ka ʻenekinia antibody a me ka hoʻomohala ʻana i ka antibody, hiki ke hoʻohana ʻia nā hale waihona hōʻikeʻike phage e nānā a hoʻomaikaʻi i nā ʻano antibody; I ka nānā ʻana i nā lāʻau lapaʻau a me ka ʻike ʻana i nā pahuhopu lāʻau, hiki ke hoʻohana ʻia nā hale waihona hōʻikeʻike phage e ʻimi i nā protein a i ʻole peptides me ka hiki ke lāʻau. I ka noiʻi lāʻau lapaʻau, hiki ke hoʻohana ʻia ka hale waihona hōʻikeʻike phage no ke koho ʻana i ka antigen lāʻau lapaʻau a me ka hoʻomohala ʻana i ka lāʻau lapaʻau. Eia kekahi, hiki ke hoʻohana ʻia nā hale waihona hōʻikeʻike phage ma nā ʻano he nui e like me ka noiʻi pilina protein, ka ʻike ʻana i ka maʻi kanesa a me ka mālama ʻana.

ʻenehana hōʻike Phage

ʻO ka ʻenehana hōʻikeʻike phage kahi ʻenehana e hoʻohui i ka gene protein target me ka gene protein surface phage, a hoʻolilo i ka protein target ma luna o ka ʻili phage ma ke ʻano o ka protein fusion ma o ka hōʻike ʻana o ka gene. Hiki i kēia mau protein i hōʻike ʻia ma luna o ka ʻili phage ke mālama i ka hoʻonohonoho spatial pili a me ka hana olaola, a hiki ke nānā ʻia, ʻike ʻia a ʻike ʻia ma ka launa pū ʻana me nā antibodies kikoʻī a i ʻole nā molekala ʻē aʻe. ʻO nā mea hōʻike o ka ʻenehana hōʻikeʻike phage e komo pū me ka antibody, ka ʻāpana antibody, ka ʻāpana peptide, cDNA, a pēlā aku.

ʻO ka ʻōnaehana o ka ʻenehana hōʻike phage e komo pū ana me ka ʻōnaehana hōʻike phage M13 (e like me ka ʻōnaehana hōʻike PⅢ, ʻōnaehana hōʻike PⅧ, a pēlā aku), ʻōnaehana hōʻike phage λ, ʻōnaehana hōʻike phage T4, ʻōnaehana hōʻike phage T7, a pēlā aku. Loaʻa i kēlā me kēia ʻōnaehana kona mau ʻano ponoʻī a kūpono no nā pono noiʻi like ʻole. ʻO ka phage M13, ma muli o kona mau waiwai lysogenic, ʻaʻole e lyse ka phage M13 i ka cell host ma hope o ka hoʻopili ʻana iā ia, e hiki ai iā ia ke hana hou a hōʻike i nā ʻāpana haole i loko o ka cell host, kūpono no ka nānā ʻana i ka wā lōʻihi a me ka hōʻike paʻa. Ma muli o kona pōʻaiapuni ola pōkole a me ka pono hana replication kiʻekiʻe, hiki i ka phage T7 ke hana koke i kahi nui o nā phage progeny, kūpono no ka nānā ʻana i ka throughput kiʻekiʻe a me ka hōʻike wikiwiki.

	M13 Phage	T7 Phage
1. ʻAno Morphological	ʻO ka phage filamentous	ʻO ka phage polyhedral
2. Mea Hoʻoilina	ʻO ka molekole DNA kaula hoʻokahi poepoe	DNA linear, kaula pālua, a me ka DNA pono ʻole loa
3. Ka Hoʻopili Hou ʻana a me ka Maʻi	Hiki ke hoʻokuʻu ʻia nā phages lysogenic, i hōʻuluʻulu ʻia i loko o ka periplasm, mai nā membrane bacteria me ka ʻole o ka lysing ʻana i nā pūnaewele host.	Hoʻokuʻu ʻia nā phages virulent, nā mamo o nā phages i hōʻuluʻulu ʻia i loko o ka cytoplasm bacteria, e ka lysis membrane cell.
4. ʻŌnaehana Hōʻike	Ua kūkulu ʻia kahi ʻōnaehana hōʻike me ka hoʻohana ʻana i nā protein capsid pIII a me PVIII, kūpono no ka hōʻike ʻana i nā peptides/protein haole o nā nui like ʻole.	Ua kūkulu ʻia ka ʻōnaehana hōʻike me ka protein capsid 10B, ʻaʻole pono ke kaʻina hana huna a kūpono no ka hōʻike ʻana i nā peptides/proteins i loaʻa ka hopena pale i ke kaʻina hana huna.
5. Pōmaikaʻi Noi	He kūpono ia no ka hōʻike a me ka nānā lōʻihi o nā peptides mole liʻiliʻi.	Loaʻa iā ia nā pono i ka hōʻike wikiwiki ʻana a me ka nānā ʻana i nā protein macromolecular kiʻekiʻe.

Nā ʻano waihona puke Peptide hōʻike Phage

He ʻike ko Alpha Lifetech i ke kūkulu ʻana a me ka nānā ʻana i ka waihona puke peptide hōʻike phage a he nui nā ʻano waihona puke peptide e koho ai. Wahi a ka lōʻihi o ka peptide, hiki ke hoʻokaʻawale ʻia i 7 mau waihona puke peptide, 9 mau waihona puke peptide, 10 mau waihona puke peptide, 12 mau waihona puke peptide, a me 15 mau waihona puke peptide. Wahi a ke ʻano spatial o nā polypeptides, hiki ke hoʻokaʻawale ʻia i ka waihona puke peptide phage linear a me ka waihona puke peptide phage cyclic.

Hale waihona puke peptide phage linear

Hoʻokomo ʻia ka gene peptide i loko o ka ʻāpana coding protein ʻaʻahu o ka genome phage, ʻo ka maʻamau he pIII a i ʻole PVIII, a hōʻike ʻia ma ke ʻano he kaulahao pololei linear. Hiki i nā peptides kaulahao linear linear ke hoʻohaʻahaʻa maʻalahi i loko o ke kino, a hiki ke hoʻopilikia ʻia ko lākou hiki ke hoʻopaʻa a me ke kūpaʻa.

waihona puke peptide phage cyclic

E hoʻokumu ka peptide apo i hoʻololi ʻia i kahi ʻano paʻa ma lalo o ka hana o nā pilina disulfide a i ʻole nā mea hoʻopaʻa ʻē aʻe, ʻaʻole wale e hoʻomaikaʻi nui i ka paʻa metabolic a me ka bioavailability, akā e hoʻokō maikaʻi hoʻi i nā koi hoʻopaʻa o ka receptor-ligand (antibody-antigen) conformation.

Kaʻina Hana Waihona Puke Peptide Hōʻike Phage

Ke koho ʻana o nā phages

ʻO ka maʻamau, koho ʻia nā phages ʻano M13 a i ʻole nā phages ʻano T7 ma ke ʻano he mau mea lawe.

Ke kūkulu ʻana i ka hale waihona puke Peptide hōʻike phage

E kūkulu i kahi vector phage i hiki ke loaʻa kahi protein haole a i ʻole kaʻina coding polypeptide, kahi e komo pū ai me kahi mea hoʻolaha, nā māka koho, a me nā mea hoʻoponopono kūpono. ʻO ka hoʻokomo ʻana o ke kaʻina coding o kahi protein haole a peptide paha i loko o kahi mea lawe phage, maʻamau ma o ka ʻoki ʻana i ka enzyme restriction a me nā ʻano ligating. Hoʻololi ʻia ka vector phage i kūkulu ʻia i loko o ke kelepona host, e ʻae ana i ka phage e hana hou a hōʻike i nā protein haole a i ʻole peptides i loko o ke kelepona. Hoʻomākaukau ʻia nā hale waihona puke phage e loaʻa ana nā protein haole like ʻole a i ʻole peptides ma o ka hoʻololi nui ʻana a me ka hoʻonui ʻana.

hōʻike phage nānā ʻana o ka Peptide Library

E hoʻopaʻa i nā molekala pahuhopu (e like me nā protein, nā mea loaʻa, a i ʻole nā antibodies) ma kahi mea lawe pae paʻa. E hoʻomoʻa i ka waihona peptide hōʻike phage i kūkulu ʻia me kahi pahuhopu paʻa e ʻae i nā peptides ma ka phage e hoʻopaʻa i ka molekala pahuhopu. I loko o kēia kaʻina hana, wehe ʻia nā bacteriophages i hoʻopaʻa ʻole ʻia ma ka holoi ʻana me ka mālama ʻana i nā bacteriophages e hoʻopaʻa i ka pahuhopu, a hoʻokuʻu ʻia nā bacteriophages i hoʻopaʻa ʻia me ka affinity kiʻekiʻe. Hoʻonui ʻia nā bacteriophages i hoʻōla ʻia ma ka hoʻopili ʻana iā Escherichia coli a laila hoʻokau ʻia i ka pōʻai aʻe o ka nānā ʻana. ʻO ka maʻamau he 3-5 mau pōʻai o ka nānā ʻana e loaʻa ai nā peptides me ka pono hoʻopaʻa kiʻekiʻe.

Hoʻolālā a me ka synthesis peptide

Hoʻohui i nā peptides e pili ana i nā wahi nānā.

Kiʻi 1 Ke kūkulu ʻana a me ke kaʻina hana nānā ʻana i ka waihona puke peptide hōʻike Phage

Paepae Pili

Hiki iā Alpha Lifetech ke hāʻawi i nā mea kūʻai aku me ke kūkulu ʻana i ka hale waihona puke peptide hōʻike phage kiʻekiʻe a me nā lawelawe nānā.

hale waihona puke antibody hōʻike phage-Alpha Lifetech

Kahua Kūkulu Hale Waihona Puke Peptide Hōʻike Phage

He nui nā ʻenehana kūkulu hale waihona puke peptide a Alpha Lifetech. Hāʻawi nui mākou i ka lawelawe kūkulu hale waihona puke peptide T7 a me ka lawelawe kūkulu hale waihona puke peptide M13 e like me nā ʻano phage.

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ka nānā ʻana i ka hōʻike phage-Alpha Lifetech ka nānā ʻana i ka hōʻike phage-Alpha Lifetech

Paepae Nānā Hale Waihona Puke Peptide Hōʻike Phage

He nui ka ʻike o Alpha Lifetech i ka nānā ʻana i nā waihona puke peptide phage, e hāʻawi ana i ke kākoʻo loea ikaika no ka ʻike ʻana i nā lāʻau lapaʻau mole liʻiliʻi, ka hōʻailona lapaʻau, ka mālama ʻana i nā maʻi a me ka noiʻi olaola.

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