ʻŌnaehana Hōʻike Phage
Ua kūkulu ʻo Alpha Lifetech i kahi ʻōnaehana hōʻike Phage paʻa kūpono e mālama ana i ka manawa no ka manawa noiʻi ʻepekema a i ʻole ka noiʻi papahana a hoʻomaʻamaʻa i ka hana ma hope.
KĀHEA IĀ MĀ˚OU01
ʻŌnaehana Hōʻike Phage
Ua komo nui ʻo Alpha Lifetech i ka ʻenehana hōʻike Phage no nā makahiki he nui. Ua kūkulu ʻo Alpha Lifetech i kahi kahua ʻenehana hōʻike Phage paʻa kūpono e mālama i ka manawa no ka manawa noiʻi ʻepekema a i ʻole ka noiʻi papahana a hoʻomaʻalahi i ka hana ʻana ma hope. Loaʻa iā mākou nā ʻōnaehana hōʻike phage he nui no nā mea kūʻai aku e koho ai, me nā ʻōnaehana hōʻike M13 phage, T7 phage, T4 phage, a me λ phage. Ma muli o kāu mau pono hoʻokolohua, hiki iā ʻoe ke koho i ka ʻōnaehana hōʻike phage kūpono. Hiki iā Alpha Lifetech ke hāʻawi i nā lawelawe o nā mea kūʻai aku e like me ka hana antibody vhh, ka hana antibody scfv, a me ka hana antibody Fab.
Hoʻolauna i ka hōʻike Phage
ʻO ka ʻenehana hōʻike Phage kahi ʻenehana e hoʻohana ana i nā phages (kahi virus e hoʻopilikia i ka bacteria) e ʻimi i nā molekala hoʻopaʻa hana o nā protein a i ʻole peptides kikoʻī. ʻO nā ʻenehana kūkulu hale waihona puke antibody phage e komo pū me ke kūkulu hale waihona puke antibody Fab, ke kūkulu hale waihona puke antibody scFv, ke kūkulu hale waihona puke antibody vhh, a pēlā aku. Wahi a nā ʻano bacteriophages, hiki ke hoʻokaʻawale ʻia i bacteriophage m13, T7 bacteriophage, T4 bacteriophage, λ bacteriophage, a me nā bacteriophage ʻē aʻe. Wahi a ke ʻano o ka hale waihona puke, hiki ke hoʻokaʻawale ʻia i loko o ka hale waihona puke peptide random, ka hale waihona puke cDNA, ka hale waihona puke antibody, a me ka hale waihona puke protein. He maʻalahi ka hana ʻana o ka ʻenehana hōʻike Phage a ʻaʻole pipiʻi ke hoʻohana ʻana i loaʻa ka hōʻike Fab phage, scfv phage display, vhh phage display. Eia nō naʻe, kaupalena kēia ʻenehana i ka ʻokoʻa o nā genetics molecular i loko o ka hale waihona puke ʻaʻole hiki ke hōʻike i nā kaʻina lōʻihi loa.
Hiki i ka ʻenehana hōʻikeʻike phage ke hana i kahi loulou kino ma waena o ka molekala i hōʻike ʻia a me ke kaʻina DNA e hoʻopili ana i ka molekala i hōʻike ʻia, a hiki ke hoʻoholo koke ʻia ke kaʻina amino acid o kahi conjugate kikoʻī e ka DNA sequencing o ka ʻāpana i makemake ʻia i loko o ka genome phage.
I kēia manawa, hoʻohana nui ʻia ka ʻenehana hōʻike phage. I waena o lākou, aia nā hoʻokō koʻikoʻi i ka noiʻi a me ka hoʻomohala ʻana i nā lāʻau pale hou (nā lāʻau pale synthetic haʻahaʻa ke kumukūʻai a me ka pono), ka hoʻomohala ʻana i nā lāʻau antibody (nā mea pale enzyme screening), ka transduction hōʻailona cell (ka nānā ʻana i nā epitopes simulated), a me ka noiʻi ʻana i nā epitopes antigen (ka hoʻomākaukau ʻana i nā antibodies monoclonal).
Kumumanaʻo o ka hōʻike ʻana o ka phage
ʻO ka hōʻike ʻana o Phage kahi ʻenehana molekala e pili ana i ka hoʻololi ʻana o ka genetic o ka phage DNA, kahi e hoʻopaʻa ai i ka ʻāpana i manaʻo ʻia i ka protein ʻaʻahu phage a pēlā e hōʻike ai ma luna o ka ʻili phage. Hoʻokomo ʻia ka moʻo DNA haole i loko o kahi kikoʻī i loko o ka moʻo nucleotide o ka genome phage e hoʻopili ana i kahi protein ʻaʻahu phage (Kiʻi 1).
Ke hana ʻia kahi maʻi phage, hoʻomaka ka gene phage e hōʻike ʻia i loko o ka host bacteria, a hōʻike ʻia ka ʻāpana i hoʻokomo ʻia ma ka ʻili o ka phage ma ke ʻano he hui pū ʻana o ka gene pili e hoʻopili ana i ka protein ʻaʻahu a me ke kaʻina cloned.
Kiʻi 1. ʻO ke ʻano hoʻolālā virion Bacteriophage M13 a me ke kumumanaʻo o ka ʻenehana hōʻike phage.(Kumu kuhikuhi: Hōʻike Phage a me nā ʻenehana hōʻike peptide ʻē aʻe | Nā Loiloi Microbiology FEMS | Oxford Academic (oup.com))
nā ʻōnaehana hōʻike phage
ʻO nā phages filamentous ka ʻōnaehana hōʻike phage i hoʻohana nui ʻia, a hiki ke kūkulu ʻia nā ʻōnaehana hōʻike phage me ka hoʻohana ʻana i nā phages ʻē aʻe. Hiki i kēlā mau ʻōnaehana ke hōʻike i nā peptides nui a nānā paha i nā hale waihona puke cDNA me ka ʻoi aku ka maikaʻi ma mua o nā ʻōnaehana phage filamentous.
Kiʻi 2 Nā ʻano bacteriophages i hoʻohana ʻia i ka hōʻike ʻana i ka phage.(Kumu kuhikuhi: Hōʻike Phage a me nā ʻenehana hōʻike peptide ʻē aʻe | Nā Loiloi Microbiology FEMS | Oxford Academic (oup.com))
Ka Pōmaikaʻi o ka ʻōnaehana hōʻike Phage
Hoʻohana pinepine nā ʻōnaehana hōʻike phage i nā phages filamentous, me M13, fd, a me f1. Ke hoʻohālikelike ʻia me nā phages M13, ua hana ʻia nā phages bacteriophage T7 me ka DNA kaula pālua, kahi e hōʻike ana i ke kūpaʻa nui a ʻaʻole i maʻalahi i ka mutation i ka wā o ka replication. ʻO ka phage T7, ma ke ʻano he mea lawe, ulu koke a hiki ke hana i nā plaques i loko o 3h, kahi e mālama ai i ka manawa nui no ka cloning a me ka screening.
Ua hoʻohana ʻia ke kūkulu ʻana o ka waihona puke phage T7 no ka hōʻike ʻana o nā gene ma luna a me ka ʻole o ka ʻili a no ke kūkulu ʻana i nā waihona hōʻike o nā peptides, cDNA, genomic DNA, a me nā antibodies. ʻAe kēia i ka T7 phage e hoʻohana ʻia ʻaʻole wale ma ke ʻano he mea antimicrobial, akā no ka nānā ʻana i ka pahuhopu lāʻau lapaʻau i ka nānā ʻana i ka pahuhopu o nā ʻōpū (e like me ka lolo, ke akepaʻa, a me ka māmā), ka nānā ʻana i ka pahuhopu ʻike antibody, ka noiʻi ʻana i ke ʻano hana physiological, a pēlā aku. Hiki ke hoʻohana ʻia no ka ʻike a me ka mālama ʻana i nā maʻi like ʻole.
Nā ʻano bacteriophage ʻē aʻe
ʻO ka phage maʻi koho
I ka hoʻohālikelike ʻana i ka hōʻike phage kuʻuna, hoʻopili ka selective infective phage (SIP) i nā pilina protein-ligand hana pololei i ka maʻi phage a me ka hoʻonui ʻana me ka ʻole o ka pono no nā ʻanuʻu elution.
Kiʻi 3. ʻO ka phage maʻi koho (SIP).(Kumu kuhikuhi: Hōʻike Phage a me nā ʻenehana hōʻike peptide ʻē aʻe | Nā Loiloi Microbiology FEMS | Oxford Academic (oup.com))
Nā phage ʻāina
Hoʻokumu ʻia nā phages ʻāina e nā tausani kope o nā peptides haole i hoʻonohonoho ʻia a puni nā capsids tubular ma nā ʻano hana hou paʻa loa, a ʻo ka hōʻike polyvalent ma nā phages ʻāina e hoʻoikaika i ke koho affinity kiʻekiʻe, no laila hiki ʻole ke hoʻokaʻawale ma waena o ka affinity kiʻekiʻe a me ka haʻahaʻa o nā ligands peptide.
Kiʻi 4 Ka manaʻo o nā phages ʻāina. (Kumu kuhikuhi: Hōʻike Phage a me nā ʻenehana hōʻike peptide ʻē aʻe | Nā Loiloi Microbiology FEMS | Oxford Academic (oup.com))
Nā ʻōnaehana hōʻike phage he nui
ʻŌnaehana hōʻike M13 phage, T7 phage, T4 phage, λ phage no nā mea kūʻai aku e koho ai.
Ka hiki ke nānā ʻana i ka throughput kiʻekiʻe
Hiki iā mākou ke hoʻohana i nā ʻōnaehana hōʻike phage e kūkulu koke i nā hale waihona puke phage nui, a hoʻokō i nā hoʻokolohua nānā ʻana me ka throughput kiʻekiʻe.
Hana hōʻike phage i hoʻopilikino ʻia
Hiki i nā mea kūʻai ke hoʻopilikino i nā lawelawe piha e like me nā koi o ka papahana, e like me ka nānā ʻana i nā antibodies affinity kiʻekiʻe, a pēlā aku.
Laulā noi ākea
Hoʻohana ʻia ma nā kahua e like me ka ʻenekinia antibody, ka ʻenekinia peptide a me ka protein, ka ʻike ʻana i nā lāʻau lapaʻau a me ka hoʻomohala ʻana, ka noiʻi pilina protein, a pēlā aku.
Inā he mau nīnau kāu, e ʻoluʻolu e hoʻokaʻaʻike mai iā mākou i kēlā me kēia manawa.
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2018-07-16 
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