Hoʻolauna i nā Hale Waihona Puke Antibody i Synthesized ʻia

ʻO ka waihona puke antibody i hoʻohuihui ʻia, i ʻike ʻia hoʻi he waihona puke De novo, he ʻano hana synthetic ia e hoʻohana ana i nā ʻano hana e like me ka synthesis DNA a i ʻole ka hōʻike phage e hoʻolālā a synthesize i nā ʻāpana loli antibody piha, me nā ʻāpana framework a me nā CDR, me ka hilinaʻi ʻole i nā waihona puke antibody naïve.

Hoʻokumu ʻia ka waihona antibody semi-synthetic ma ka hoʻohui ʻana i nā waihona antibody kūlohelohe me nā ʻano like ʻole synthetic. ʻO ka maʻamau, pili ia i ka synthesizing DNA oligonucleotides e hana i kahi hoʻonohonoho o nā ʻāpana hoʻoholo hoʻohui like ʻole (CDR), a laila hui pū ʻia me kahi ʻōnaehana antibody mau i loaʻa mai nā kumu kūlohelohe e like me nā cell B kanaka a holoholona paha. Hoʻokomo ka CDR synthesized i ka ʻokoʻa i loko o ka waihona, e hopena ana i nā antibodies e kuhikuhi ana i kahi ākea o nā epitopes. Hāʻawi ka waihona antibody semi-synthetic i kahi ʻaelike ma waena o ka ʻokoʻa kūlohelohe o ka ʻōnaehana pale a me ka ʻokoʻa i kāohi ʻia i loaʻa ma o nā ʻano synthetic.

ʻO ka ʻokoʻa ma waena o nā waihona puke antibody naïve a me synthetic e pili ana i ke kumu o nā genes immunoglobulin. I kēia manawa, ʻo ka ʻokoʻa ma waena o kahi waihona puke antibody semi-synthesized a synthesized aia ma ka mea mua he ʻāpana o nā ʻāpana antibody naïve, e like me ke kaulahao māmā a i ʻole ke kaulahao kaumaha, a hoʻokahi wale nō o lākou i synthesized in vitro; ʻoiai ʻo ka mea i synthesized ʻia ua loaʻa piha mai kahi synthesis artificial e PCR in vitro.

Hiki iā Alpha Lifetech ke hāʻawi

ʻO Alpha Lifetech Inc.hiki ke hāʻawi i kahi waihona puke antibody semi-synthesized/synthesized mai nā holoholona a me nā kānaka e pili ana i kā mākou kahua ʻike antibody ʻoihana. Me nā makahiki o ka ʻike i ka hoʻololi ʻana i ka genetic a me ke kūkulu ʻana i ka waihona puke, hiki ke kūkulu ʻia nā waihona puke synthesized i hiki ke hana i nā antibodies synthetic me ka pili a me ke kikoʻī ma mua o ka hiki o nā antibodies kūlohelohe.ʻO Alpha LifetechUa haʻaheo ka poʻe loea e hoʻolaha e hiki iā mākou ke hōʻoia i kahi kūleʻa kiʻekiʻe i ke kūkulu ʻana i kahi waihona antibody e loaʻa ana he 10^8 - 10^10 mau clones kūʻokoʻa.

ʻO Alpha Lifetech Inc.haʻaheo e hāʻawi i nā lawelawe kūkulu hale waihona puke antibody piha e hoʻokō i nā pono like ʻole o nā mea noiʻi honua, me scFv, Fab, VHH antibody, a me nā hale waihona puke i hoʻopilikino ʻia. ʻO kā mākou pahuhopu ka hoʻomaopopo a hoʻokō i nā koi o nā mea kūʻai aku like ʻole a kōkua i nā pilikia e hiki mai ana a me nā pilikia e kū mai ana i ka hana noiʻi.

Kaʻina Hana Hana Kūkulu Hale Waihona Puke Antibody Synthesized

Hoʻolālā
ʻO ka pae hoʻolālā e pili ana i ke koho ʻana i nā ʻōnaehana antibody a me nā ʻāpana e hoʻoholo ana i ka hoʻopihapiha (CDR) e pili ana i nā kaʻina antibody e kū nei a i ʻole ka ʻikepili hoʻonohonoho. Hiki ke hoʻolālā ʻia nā antibodies CDR synthetic e hoʻolauna i nā hana kikoʻī a hoʻomaikaʻi paha i nā waiwai hoʻopaʻa.

Hoʻohuihui
Hoʻohui ʻia ka DNA synthetic e hoʻopili ana i nā moʻo antibody i hoʻolālā ʻia, me nā ʻāpana ʻōnaehana a me nā CDR, me ka hoʻohana ʻana i nā ʻano kemika a i ʻole enzymatic.

ʻAhaʻōlelo
Hoʻākoakoa ʻia nā ʻāpana DNA i hana ʻia i loko o nā vectors hōʻike antibody me ka hoʻohana ʻana i nā ʻano hana e like me PCR, ligation, a i ʻole Gibson assembly. A laila hiki ke hoʻokomo ʻia kēia mau vectors i loko o nā ʻōnaehana hōʻike e like me nā cell bacteria, yeast, a i ʻole mammalian no ka hana ʻana i ka antibody.

Nānā a me ke Koho ʻana
Ua nānā ʻia a koho ʻia nā waihona antibody i kūkulu ʻia no nā antibodies me nā waiwai i makemake ʻia me ka hoʻohana ʻana i nā ʻano loiloi throughput kiʻekiʻe. Hiki i kēia ke pili i nā ʻano hana e like me ka hōʻike phage, ka hōʻike yeast, a i ʻole ka hōʻike ribosome, ma muli o ke ʻano o ka waihona a me ka noi.

Hana Kūkulu Hale Waihona Puke Antibody Synthesized

ʻO Alpha Lifetech Inc.Hāʻawi i nā mea kūʻai aku i kahi hoʻolālā hoʻokahi a me ka lawelawe synthesized o nā hale waihona puke antibody ma o ka hoʻopili ʻana i nā repertoires V-gene kaulahao kaumaha a me ka māmā i loko o kahi vector phage me ka hoʻohana ʻana i ka ʻōnaehana recombination kikoʻī Cre-lox e hana i kahi repertoire o Fabs i hōʻike ʻia ma ka phage e loaʻa ana he 6.5 × 10^10 clones. Ua hāʻawi ka hale waihona puke iā Abs e kūʻē i nā antigens he nui, kekahi me nā affinities nanomolar. Hoʻokumu ʻia ka hanauna o ka hale waihona puke antibody kanaka ma ka ʻenehana hōʻike phage M13 (hōʻike ʻia ma ke kiʻi ma lalo).