Introduction to Synthesized Antibody Libraries

ʻO ka hale waihona antibody Synthesized, i ʻike ʻia he hale waihona De novo, he ʻano hana synthetic e hoʻohana ana i nā ʻenehana e like me ka DNA synthesis a i ʻole hōʻike phage e hoʻolālā a synthesize i nā ʻāpana ʻokoʻa antibody piha, me nā ʻāpana framework a me nā CDR, me ka hilinaʻi ʻole i nā hale waihona antibody naïve.

Hoʻokumu ʻia ka waihona antibody semi-synthetic ma o ka hoʻohui ʻana i nā hale waihona antibody kūlohelohe me nā ʻano like ʻole. Hoʻopili maʻamau ia i ka synthesizing DNA oligonucleotides e hana i kahi pūʻulu o nā ʻāpana koho hoʻohui like ʻole (CDRs), a laila hui pū ʻia me kahi framework antibody mau i loaʻa mai nā kumu kūlohelohe e like me ke kanaka a holoholona B cell. Hoʻokomo ka CDR synthesized i ka ʻokoʻa i loko o ka hale waihona puke, ka hopena i nā antibodies e huli ana i kahi ākea o nā epitopes. Hāʻawi ka hale waihona antibody semi-synthetic i ka ʻokoʻa ma waena o ka ʻokoʻa kūlohelohe o ka ʻōnaehana pale a me ka ʻokoʻa i hoʻopaʻa ʻia i loaʻa ma o nā ʻano hana synthetic.

ʻO ka ʻokoʻa ma waena o nā hale waihona puke antibody naïve a me synthetic ma muli o ke kumu o nā genes immunoglobulin. I kēia manawa, ʻo ka ʻokoʻa ma waena o kahi hale waihona antibody semi-synthesized a synthesized aia ma ka mea mua he ʻāpana o nā ʻāpana antibody naïve, e like me ke kaulahao māmā a i ʻole ke kaulahao kaumaha, a hoʻokahi wale nō o lākou i synthesized in vitro; ʻoiai ʻo ka mea synthesized i loaʻa loa mai kahi synthesis artificial e PCR in vitro.

Hiki iā Alpha Lifetech ke hāʻawi

Alpha Lifetech Inc.hiki ke hāʻawi i kahi waihona antibody semi-synthesized/ synthesized mai nā holoholona a me nā kānaka e pili ana i kā mākou kahua ʻike antibody ʻoihana. Me nā makahiki o ka ʻike i ka hoʻololi genetic a me ka hana hale waihona puke, hiki ke kūkulu ʻia nā hale waihona puke synthesized i hiki ke hana i nā antibodies synthetic me ka pili a me ka kikoʻī ma mua o ka hiki o nā antibodies kūlohelohe.ʻO Alpha Lifetech'shaʻaheo ka poʻe akamai e hoʻolaha e hiki iā mākou ke hōʻoiaʻiʻo i ka helu kūleʻa kiʻekiʻe ma ke kūkulu ʻana i kahi waihona antibody i loaʻa nā clones kūʻokoʻa 10^8 – 10^10.

Alpha Lifetech Inc.Haʻaheo ʻo ia e hāʻawi i nā lawelawe kūkulu hale waihona puke antibody e hoʻokō i nā pono like ʻole o nā mea noiʻi honua, me ka scFv, Fab, VHH antibody, a me nā hale waihona puke maʻamau. ʻO kā mākou pahuhopu e hoʻomaopopo a hoʻokō i nā koi o nā mea kūʻai aku like ʻole a me ke kōkua ʻana i nā pilikia e hiki mai ana a e kū mai ana i ka hana noiʻi.

Ka Hana Hana Hana Hana Hana Hale Waihona Puke Antibody Synthesized

Hoʻolālā
ʻO ka hana hoʻolālā e pili ana i ke koho ʻana i nā ʻāpana antibody a me nā ʻāpana hoʻoholo hoʻohui (CDRs) e pili ana i nā kaʻina antibody i loaʻa a i ʻole ka ʻikepili i kūkulu ʻia. Hiki ke hoʻolālā ʻia nā antibodies CDR Synthetic e hoʻolauna i nā hana kikoʻī a hoʻomaikaʻi paha i nā waiwai paʻa.

Hoʻohuihui
Hoʻopili ʻia ka DNA Synthetic i nā kaʻina antibody i hoʻolālā ʻia, me nā ʻāpana framework a me CDRs, i hoʻohana ʻia me ka hoʻohana ʻana i nā ʻano kemika a enzymatic.

Ahahui
Hoʻohui ʻia nā ʻāpana DNA synthesized i nā vector hōʻike antibody me ka hoʻohana ʻana i nā ʻenehana e like me PCR, ligation, a i ʻole hui ʻo Gibson. Hiki ke hoʻokomo ʻia kēia mau vectors i nā ʻōnaehana hōʻike e like me ka bacteria, yeast, a i ʻole nā ​​​​pūnaewele mammalian no ka hana antibody.

Nānā a koho
Hoʻopili ʻia nā hale waihona puke antibody i kūkulu ʻia a koho ʻia no nā antibodies me nā waiwai i makemake ʻia me ka hoʻohana ʻana i nā ʻano screening high-throughput. Hiki i kēia ke komo i nā ʻenehana e like me ka hōʻike phage, hōʻike hū, a i ʻole hōʻike ribosome, ma muli o ke ʻano o ka waihona a me ka noi.

ʻO ka lawelawe kūkulu hale waihona puke ʻo Synthesized

Alpha Lifetech Inc.Hāʻawi i nā mea kūʻai aku i kahi hoʻolālā hoʻokahi a me ka lawelawe synthesized o nā hale waihona antibody ma o ka hoʻopaʻa ʻana i nā repertoires V-gene kaumaha a māmā i loko o kahi vector phage me ka hoʻohana ʻana i ka ʻōnaehana hoʻohui pūnaewele ʻo Cre-lox e hana i kahi repertoire o Fabs i hōʻike ʻia ma ka phage me 6.5 × 10^10 clones. Hāʻawi ka waihona iā Abs i nā antigens he nui, ʻo kekahi me nā pili nanomolar. Hoʻokumu ʻia ka hanauna o ka waihona antibody kanaka ma ka ʻenehana hōʻike M13 phage (hōʻike ʻia ma ke kiʻi ma lalo).