Hana Kūkulu Hale Waihona Puke Hōʻikeʻike Hū

He loea ʻo Alpha Lifetech i ke kūkulu ʻana i ka hale waihona hōʻikeʻike hū a me ka nānā ʻana i nā hale waihona antibody, e hāʻawi ana i ka hiki ke hana i nā ʻano like ʻole o nā hale waihona hū a nānā iā lākou no ka pilina kiʻekiʻe a me nā antibodies kikoʻī no nā mea kūʻai aku ma ka honua holoʻokoʻa. Me kahi hui o nā mea noiʻi akamai, nā ʻenehana holomua a me nā lako, hiki iā mākou ke kūkulu i nā hale waihona hū e pili ana i nā ʻano protein e pili ana i ka maʻi.

Ka hope o ka ʻōnaehana hōʻike hū

ʻO ka hōʻike ʻana i ka hū he ʻenehana hōʻike protein eukaryotic e hoʻohana ana i ka ʻōnaehana hoʻololi protein intracellular (GPI anchor) o ka ʻōnaehana hōʻike ʻili hū e hoʻopaʻa a hōʻike i nā antibodies exogenous a i ʻole nā gene protein ma ka ʻili hū. Loaʻa i ka ʻōnaehana hōʻike ʻili hū kahi ʻano hoʻololi a me ka hoʻokuʻu ʻana o ka protein ma hope o ka unuhi ʻana, ʻoi aku ka kūpono no ka pelu ʻana, ka hoʻololi ʻana a me ka lawe ʻana i nā protein humanized. No laila, ʻoi aku ka kokoke o nā antibodies i hana ʻia e ka ʻōnaehana hōʻike hū i nā antibodies maoli kanaka ma ke ʻano a me ka hana, a hāʻawi i ka hiki ke hoʻohiki no nā noi mālama maʻi.

ʻO ka ʻōnaehana hōʻike hū e hiki ai i ka hana ʻana o nā antibodies kūikawā no nā protein, peptides, nā mole liʻiliʻi, a me nā mea loaʻa T-cell. Loaʻa i kēia mau antibodies nā pono o ka versatility, quantification a me ka therapeutic efficacy. ʻOi aku ka laulā o ka hoʻohana ʻana o ka ʻenehana hōʻike hū, hiki ke hoʻohana ʻia ʻaʻole wale no ka ʻike ʻana i ka antibody hōʻike hū, akā no ka nānā ʻana i ka waihona puke protein, ka hoʻomohala ʻana i kuhikuhi ʻia e ka protein, ke kūkulu ʻana i ka waihona puke antigen, ka hoʻokaʻawale ʻana i ka antibody affinity kiʻekiʻe, ka hoʻomānoanoa ʻana o ka antibody affinity, ka hana ʻana i ka lāʻau lapaʻau, ka immuno-biocatalysis, a me nā biosensors, a pēlā aku, nona kahi manaʻolana ākea ma nā kahua o ka hōʻoia ʻana, ka mālama ʻana i nā maʻi a me ka noiʻi lapaʻau. Eia kekahi, ʻoi aku ka kokoke o nā protein i hoʻomākaukau ʻia ma o ka hōʻike ʻana i ka ʻili hū antigenic i ko lākou ʻano kūlohelohe, e kūpono ai lākou no nā assays serological e like me ELISA. hōʻike hū-Alpha Lifetech

Kiʻi 1: Kumumanaʻo hōʻike hū

Kaʻina hana kūkulu hale waihona hōʻikeʻike hū

Loaʻa i kā mākou mau waihona hū ka nui o ka mana a me ke ʻano like ʻole, e hāʻawi ana i kahi kahua kiʻekiʻe no ka nānā pono ʻana i nā moʻo antibody kikoʻī. Hiki iā mākou ke kūkulu i nā ʻano like ʻole o nā waihona antibody hū (IgG, scFv, VHH, Fab antibody libraries), nā waihona protein, nā waihona peptide, nā waihona cDNA, a pēlā aku e like me kāu mau pono. Eia kekahi, hiki iā mākou ke hoʻomohala i nā waihona antibody o nā waiwai like ʻole, me nā waihona immune, nā waihona kūlohelohe, nā waihona synthetic, nā waihona semi-synthetic, a me nā waihona antibody maʻi. ʻO pYD kā mākou vector hōʻikeʻike ʻili hū i hoʻohana pinepine ʻia.

Hiki iā mākou ke kūkulu i nā hale waihona puke hū me ka hiki ke hoʻopaʻa ʻia a hiki i ka 10^9, me ka 100% ka nui o ka hoʻokomo ʻana a me ke ʻano like ʻole. Ma mua o ka hāʻawi ʻana i ka hale waihona puke hū, hōʻoia pono mākou e hōʻoia i ka ʻokoʻa, ka pono a me ka hana o nā genes antibody. I mea e hōʻoia ai he kiʻekiʻe loa ka pilina a me ke kikoʻī o nā antibodies i nānā ʻia, hoʻohana mākou i ka loiloi quantitative manawa maoli o ka hale waihona puke antibody. Nānā nui ʻia ka hoʻokaʻawale ʻana o nā cell fluorescence-activated (FACS) no nā loli i ka hōʻike ʻana a me ka hana antibody, kahi e hoʻohana ai i nā māka ʻokoʻa ma ka antibody a me ka antigen e hoʻoholo i ka ikaika o ka hoʻopaʻa ʻana a me ka hōʻike ʻana o ka antibody ma ka ʻili o nā cell hū. Hoʻohana mākou i nā hoʻāʻo hoʻopaʻa FACS e nānā nui no nā antibodies me ka pilina kiʻekiʻe a me ke koho ʻana. ʻOi aku ka maikaʻi o ka hōʻike ʻana o kā mākou hale waihona puke nanobody hōʻike hū e FACS ma mua o 95%. ʻO ka maʻamau, pili ka nānā ʻana i ka hū i 3-5 mau puni o ka nānā ʻana a me ka hoʻonui ʻana, e ʻae ana iā mākou e loaʻa nā antibodies affinity antibody kiʻekiʻe i kahi manawa pōkole.

Ua lilo ka ʻōnaehana hōʻike hū i mea hana ikaika i ka ulu ʻana o ka protein i kuhikuhi ʻia. Hāʻawi ʻo Alpha Lifetech i nā lawelawe hōʻike protein, hoʻomaʻemaʻe, a me ka loiloi kinetic expression no ka hoʻomākaukau ʻana i ka antibody kikoʻī in vitro. Loaʻa iā mākou ka hiki ke hoʻomākaukau i nā protein nui a hoʻomaikaʻi pono i ka maʻemaʻe o nā protein recombinant. Hāʻawi mākou i ka lawelawe hoʻokahi-kū mai ka synthesis gene a hiki i ka hōʻike protein recombinant a me ka hoʻomaʻemaʻe. Me ka hoʻohana ʻana i kā mākou ʻōnaehana hōʻike hū holomua, ua hoʻomākaukau pono mākou i nā antibodies me ka picomolar kiʻekiʻe loa (pM) affinity, ʻo ia kahi holomua nui i ka ʻenehana hoʻomākaukau antibody recombinant in vitro.