Aptamer Development Platform

Aptamers yog ib leeg-stranded oligonucleotide (DNA, RNA lossis XNA) nrog cov cuab yeej ntawm kev sib raug zoo siab thiab muaj kev tshwj xeeb uas tshwj xeeb khi rau lub hom phiaj molecules zoo li cov tshuaj tiv thaiv, thiab lawv tau siv dav rau kev txhim kho biosensors, kuaj mob thiab kho.

Lub aptamer platform muab los ntawm Alpha Lifetech suav nrog ob pawg: aptamer synthesis platform, uas feem ntau koom nrog SELEX aptamer tsev qiv ntawv synthesis kev pabcuam thiab aptamer (DNA, RNA lossis XNA) kev pabcuam kev loj hlob, thiab aptamer tshuaj ntsuam platform nrog rau kev tshuaj xyuas kev pabcuam raws li SELEX thev naus laus zis rau cov protein, peptides, hlwb, lub hom phiaj me me thiab cov hlau molecules. Aptamer optimization thiab txheeb xyuas cov kev pabcuam.

Aptamer Synthesis Platform

SELEX Aptamer Library Synthesis Service

SELEX aptamer lub tsev qiv ntawv synthesis cov kev pabcuam feem ntau yog tsim lub tsev qiv ntawv uas muaj ntau ntawm cov random ib leeg-stranded oligonucleotide sequences los ntawm in vitro tshuaj synthesis raws li lub hom phiaj moleculars. Kev tsim tsev qiv ntawv yog qhov pib ntawm SELEX thev naus laus zis, uas muab ntau tus neeg sib tw ua ntu zus rau cov txheej txheem tshuaj ntsuam tom ntej los ntawm kev tsim cov tsev qiv ntawv loj loj thiab ua rau muaj peev xwm tshuaj xyuas cov neeg sib tw siab.

Library synthesis feem ntau muab faib ua cov kauj ruam hauv qab no:

Cov kauj ruam	Technology Paub meej
Txheeb xyuas Lub Hom Phiaj Molecules	Txheeb xyuas lub hom phiaj molecules uas yuav tsum tau soj ntsuam rau aptamers, uas tuaj yeem yog cov proteins, nucleic acids, me me molecular, hlau ions, thiab lwm yam.
Random Sequence Design	Random sequence ntev, lub hauv paus muaj pes tsawg leeg thiab lwm yam tsis tau tsim raws li cov yam ntxwv ntawm lub hom phiaj molecules thiab kev soj ntsuam yuav tsum tau. Feem ntau, random sequences nyob nruab nrab ntawm kaum thiab pua pua ntawm qhov ntev.
Synthesis of Taag Sequences	Oligonucleotide fragments nrog cov kab ke ruaj khov (xws li PCR primer sequences) ntawm ob qhov kawg yog tsim thiab ua ke, uas yuav siv rau hauv kev nthuav dav thiab kev tshuaj ntsuam tom ntej.

Lub tsev qiv ntawv synthesized tseem yuav tsum tau ua ntxiv rau kev tswj kom zoo. Qhov concentration ntawm lub tsev qiv ntawv tau txiav txim siab los xyuas kom meej tias nws muaj feem xyuam rau hauv cov txheej txheem tshuaj ntsuam tom ntej. Qhov sib txawv thiab qhov tseeb ntawm qhov sib txawv hauv cov tsev qiv ntawv tau raug txheeb xyuas los ntawm kev sib cais thiab lwm txoj hauv kev los xyuas kom meej tias lub tsev qiv ntawv zoo tau ua raws li kev tshuaj ntsuam xyuas.

Los ntawm cov kauj ruam saum toj no, lub tsev qiv ntawv zoo tshaj plaws thiab muaj ntau haiv neeg SELEX aptamer tuaj yeem ua ke, uas tuaj yeem muab ntau tus neeg sib tw ua ntu zus rau cov txheej txheem tshuaj ntsuam tom ntej.

Aptamer txoj kev pabcuam (DNA, RNA lossis XNA)

Aptamers feem ntau xa mus rau nucleic acid aptamers. Nucleic acid aptamers incudes DNA aptamers, RNA aptamers, thiab XNA aptamers uas yog tshuaj hloov kho nucleic acid aptamers. Cov txheej txheem SELEX yog dav siv rau kev txhim kho ntawm aptamers. Cov kev ua haujlwm yooj yim ntawm cov kev pabcuam kev txhim kho aptamer suav nrog kev tsim tsev qiv ntawv, lub hom phiaj khi, kev sib cais thiab kev ua kom huv si, kev nthuav dav, ntau qhov kev tshuaj ntsuam xyuas, thiab kev txheeb xyuas cov kab ke. Tau ntau xyoo, peb tau tsom mus rau kev tsim tsev qiv ntawv thiab kev paub nplua nuj hauv kev txhim kho aptamer. Peb cog lus tias yuav muab cov kev pabcuam zoo dua rau cov neeg siv khoom.

SELEX Technology Txheej Txheem

Cov txheej txheem SELEX muaj ntau qhov sib tw, txhua qhov uas muaj cov kauj ruam tseem ceeb hauv qab no:

Library thiab Target Binding

Lub tsev qiv ntawv tsim nucleic acid yog sib xyaw nrog cov hom phiaj tshwj xeeb (xws li cov proteins, cov khoom sib xyaw me me, thiab lwm yam), kom cov nucleic acid sequences hauv lub tsev qiv ntawv muaj lub sijhawm los khi rau lub hom phiaj molecules.

Kev rho tawm ntawm Unbound Molecules

Nucleic acid sequences uas tsis yog khi rau lub hom phiaj molecule yog sib cais los ntawm kev sib tov los ntawm cov kev tshwj xeeb xws li affinity chromatography, sib nqus hlaws sib cais, thiab lwm yam.

Amplification ntawm Binding Molecules

Cov nucleic acid ib ntus ua ke rau lub hom phiaj molecule yog amplified, feem ntau yog siv polymerase saw cov tshuaj tiv thaiv (PCR) technology. Rau qhov kev tshuaj ntsuam tom ntej no, cov kab ke nthuav dav yuav raug siv los ua lub tsev qiv ntawv pib.

Daim duab 1: SELEX kev tshuaj ntsuam xyuas

Aptamer Screening Platform

Aptamer kev tshuaj ntsuam xyuas

Alpha Lifetech muaj ntau hom kev pabcuam tshwj xeeb aptamer tshuaj xyuas siv ntau yam SELEX cov txheej txheem rau ntau hom ntawm koj cov molecules:

Hom phiaj	Cov ntsiab lus technical
Protein Aptamer Screening los ntawm SELEX	Lub hom phiaj tseem ceeb ntawm kev ntsuam xyuas cov protein aptamer yog los tshuaj xyuas cov aptamers uas tuaj yeem khi tshwj xeeb rau lub hom phiaj ntawm cov protein molecules. Cov aptamers no yooj yim rau kev sib txuas, ruaj khov thiab tsis tshua muaj kev cuam tshuam rau ib puag ncig.
Peptide Aptamer Screening los ntawm SELEX	Peptide aptamers yog ib chav kawm ntawm luv luv peptide sequences nrog siab tshwj xeeb thiab affinity, uas muaj peev xwm tshwj xeeb khi rau lub hom phiaj tshuaj thiab qhia ntau yam ntawm daim ntawv thov muaj peev xwm nyob rau hauv biomedical teb. Los ntawm cov txheej txheem kev tshuaj xyuas tshwj xeeb, peptide aptamers uas tuaj yeem khi rau lub hom phiaj molecules raug tshuaj xyuas los ntawm ntau lub tsev qiv ntawv random peptide.
Cell-Specific Aptamer Screening (Cell-SELEX)	Lub hom phiaj lossis cov molecules tshwj xeeb ntawm lub xov tooj ntawm tes tau npaj ua lub hom phiaj. Cov hom phiaj tuaj yeem yog tag nrho cov hlwb, receptors ntawm cov cell membrane, cov proteins, lossis lwm cov khoom me me.
Me Me Molecule Aptamer Screening los ntawm Capture SELEX	Capture SELEX yog ib qho kev tshuaj ntsuam hauv vitro rau kev tshuaj xyuas ntawm cov molecule aptamers me, uas yog ib qho txawv ntawm SELEX. Capture SELEX yog qhov tshwj xeeb zoo rau kev tshuaj ntsuam aptamer ntawm cov hom phiaj me me, uas feem ntau muaj cov pab pawg ua haujlwm tsawg dua thiab nyuaj rau immobilization ncaj qha ntawm cov khoom txhawb nqa theem.
Nyob Tsiaj-raws li kev pabcuam SELEX	Kev kuaj tsiaj nyob hauv tsev yog ib qho kev sim uas siv dav hauv thaj chaw bioscience, tshuaj thiab biotechnology, uas siv cov tsiaj muaj sia los ua qauv sim los tshuaj xyuas thiab ntsuas cov molecules tshwj xeeb, kws kho mob, kho lossis cov txheej txheem lom neeg. Cov kev pabcuam yog tsim los simulate lub cev ib puag ncig hauv tib neeg lub cev kom raug kwv yees ntau dua thiab ntsuas qhov ua tau zoo thiab kev nyab xeeb ntawm kev sim cov txiaj ntsig hauv tib neeg lub cev.

Aptamer optimization kev pab cuam

Lub hydrophilicity, siab affinity poob thaum lub sij hawm tsim khoom, thiab ceev excretion ntawm aptamers txwv lawv daim ntawv thov. Tam sim no, ntau yam kev ua kom zoo tau raug tshawb nrhiav los txhim kho kev ua haujlwm ntawm aptamers.

Peb kuj muaj ntau txoj hauv kev los txhim kho aptamer muaj xws li truncation, kev hloov kho, conjugation rau pawg neeg tsim nyog (thiol, carboxy, amine, fluorophore, thiab lwm yam).

Aptamer characterization kev pabcuam

Aptamer characterization tsom xam qhov kev pab cuam yog hais txog cov kev pab cuam ntawm kev soj ntsuam kev ua tau zoo qauv kev daws teeb meem thiab kev ua hauj lwm pov thawj ntawm cov tau txais aptamer los xyuas kom meej tias lub aptamer ua tau raws li qhov tshwj xeeb binding muaj peev xwm, stability thiab specifications. Nws feem ntau suav nrog affinity thiab kev tsom xam tshwj xeeb, kev ntsuas kev ruaj ntseg thiab kev txheeb xyuas kev ua haujlwm lom neeg.