Lub Platform Tsim Tshuaj Tiv Thaiv Kab Mob Bispecific

Alpha Lifetech muaj peev xwm muab cov neeg siv khoom nrog kev tshawb pom cov tshuaj tiv thaiv kab mob zoo thiab cov khoom lag luam thiab cov kev pabcuam recombinant protein. Peb tuaj yeem npaj cov tshuaj tiv thaiv kab mob nrog kev ua haujlwm zoo, muaj zog tshwj xeeb, thiab ruaj khov zoo. Alpha Lifetech muaj ntau yam cuab yeej thiab khoom siv ntxuav cov tshuaj tiv thaiv kab mob, uas tuaj yeem muab cov kev pabcuam ntxuav cov tshuaj tiv thaiv kab mob los ntawm ntau qhov chaw xws li luav, yaj, qaib, thiab nas monoclonal antibodies, nrog rau Protein A/G affinity purification services thiab antibody separation thiab purification services. Raws li kev tsim kho platform system ntawm antibody discovery platform, protein platform, thiab lwm yam, peb npog cov kev pabcuam upstream thiab downstream ntawm kev tsim cov tshuaj tiv thaiv kab mob, thiab tuaj yeem muab cov kev pabcuam kev tshaj lij los ntawm kev npaj cov tshuaj tiv thaiv kab mob, bispecific antibody purification thiab antibody sequencing, antibody validation, thiab lwm yam, uas tuaj yeem siv rau kev kho mob bispecific antibody.

Cov tshuaj monoclonal antibodies yog hais txog cov immunoglobulins uas tsim los ntawm ib lub B cell uas muaj qhov tshwj xeeb rau cov antigens lossis epitopes. Kev tsim cov tshuaj monoclonal antibodies tau pib npaj siv cov thev naus laus zis hybridoma los tsim cov tshuaj tiv thaiv nas. Tshwj xeeb, los ntawm kev sib xyaw cov hlwb spleen los ntawm cov nas uas tau txhaj tshuaj tiv thaiv kab mob nrog cov hlwb myeloma los ntawm tib neeg lossis nas, cov hlwb hybridoma tau tsim, uas tso tawm cov tshuaj tiv thaiv tshwj xeeb. Cov tshuaj monoclonal antibodies uas tsim los ntawm nas feem ntau yog siv rau hauv kev tshawb fawb tsiaj thiab kev kuaj mob tom qab kev pabcuam ntxuav. Txawm li cas los xij, lub cev tib neeg yuav tsim cov lus teb tiv thaiv kab mob rau cov protein nas txawv teb chaws, yog li muaj cov kev txwv tseem ceeb hauv kev siv tshuaj monoclonal antibodies nas. Tom qab ntawd, tib neeg siv "humanization" los npaj cov tshuaj monoclonal antibodies los ntawm nas, thiab siv cov thev naus laus zis genetic engineering los hloov cov tshuaj nas kom muaj thaj chaw tas li ntawm cov tshuaj immunoglobulin tib neeg kom txo lawv cov immunogenicity. Hom tshuaj antibody no kuj hu ua humanized monoclonal antibody. Tsis tas li ntawd, kev siv cov hlwb tib neeg los tsim cov tshuaj monoclonal antibodies hu ua tag nrho cov tib neeg monoclonal antibody npaj. Cov tshuaj monoclonal antibodies tau siv dav hauv kev siv biomedical thiab kev kho mob. Hauv kev tshawb fawb kho mob, cov tshuaj monoclonal antibodies tuaj yeem siv ua cov tshuaj immunomodulators, cov tshuaj monoclonal antibodies kho mob tuaj yeem siv rau kev kho mob qog noj ntshav thiab kev kho mob kis kab mob, thiab kev tsim cov monoclonal antibody conjugates feem ntau yog siv rau kev kho mob qog noj ntshav.

Kev Taw Qhia Txog Bispecific Antibody

Xyoo 1960, lub tswv yim ntawm cov tshuaj tiv thaiv kab mob bispecific tau raug tsim tawm. Cov tshuaj tiv thaiv kab mob bispecific, tseem hu ua cov tshuaj tiv thaiv kab mob bispecific monoclonal, yog cov tshuaj tiv thaiv kab mob uas tau tsim los ntawm kev siv tshuab genetic engineering. Raws li cov tshuaj tiv thaiv kab mob uas tau tsim, cov tshuaj tiv thaiv kab mob bispecific feem ntau yog cov khoom siv IgG subclass thiab muaj cov khoom sib txuas antigen uas tsom mus rau CD3 subunit. Cov tshuaj tiv thaiv kab mob bispecific muaj ob qhov chaw sib txuas antigen tshwj xeeb uas tuaj yeem khi thiab paub ob lub antigens sib txawv, lossis ob lub epitopes sib txawv ntawm ib lub antigen. Piv nrog cov tshuaj tiv thaiv kab mob monoclonal, cov tshuaj tiv thaiv kab mob bispecific muaj qhov chaw sib txuas antigen tshwj xeeb ntxiv, yog li muaj qhov tshwj xeeb thiab lub peev xwm tsom mus rau, uas tuaj yeem tsom mus rau cov hlwb qog nqaij hlav kom raug dua thiab txo qhov lom ntawm lub hom phiaj. Cov tshuaj tiv thaiv kab mob bispecific tuaj yeem ua ntau yam haujlwm biological tib lub sijhawm, xws li nrhiav cov hlwb tiv thaiv kab mob, thaiv cov kev taw qhia, thiab tua cov hlwb qog nqaij hlav ncaj qha. Cov tshuaj tiv thaiv kab mob bispecific thaum ntxov feem ntau tau npaj los ntawm kev sib xyaw tshuaj lossis kev sib xyaw ua ke ntawm cov hlwb, tab sis txoj kev no yuav tau nce qib qeeb vim yog kev sib xyaw ua ke thiab nyuaj rau kev cais cov kev sib xyaw ua ke. Nrog rau kev txhim kho tas mus li ntawm cov thev naus laus zis genetic engineering, ntau lub platform thev naus laus zis tshiab tau tsim, xws li cov pob caus hauv qhov (KIH), CrossMab, DVD Ig, thiab lwm yam. Cov platform no daws tau cov teeb meem xws li cov saw hlau hnyav thiab cov saw hlau sib txawv, thiab txhim kho qhov sib xws thiab cov txiaj ntsig ntawm cov tshuaj tiv thaiv bispecific.

Daim Duab 1 Daim duab qhia txog cov txheej txheem ua haujlwm rau cov tshuaj tiv thaiv kab mob bispecific (bsAbs) hauv kev sim tshuaj rau kev kho mob qog noj ntshav.(Duab los ntawm: Kev tshuaj xyuas cov tshuaj tiv thaiv kab mob bispecific thiab cov qauv tshuaj tiv thaiv kab mob hauv kev kho mob oncology thiab kev cov nyom hauv kev kho mob - ScienceDirect)

Kev Tsim Khoom Siv Antibody Bispecific

Cov txheej txheem tseem ceeb rau kev tsim cov tshuaj tiv thaiv kab mob bispecific suav nrog kev sib txuas tshuaj lom neeg, plaub qhov chaw hybridoma, thiab kev npaj tshuaj tiv thaiv kab mob genetic engineering. Ntawm lawv, txoj kev sib txuas tshuaj lom neeg sib txuas ob qho IgG lossis ob qho F (ab ') 2 antibody fragments rau hauv cov tshuaj tiv thaiv kab mob bispecific siv cov tshuaj sib txuas tshuaj lom neeg xws li phthalimide thiab dithioacylbenzoic acid. Txoj kev no yooj yim thiab yooj yim rau kev ua haujlwm, tab sis nws yuav ua rau puas tsuaj rau qhov chaw khi antigen, txo cov haujlwm antibody, thiab tus neeg sawv cev sib txuas nws tus kheej kuj muaj qee qib ntawm carcinogenicity. Txoj kev hybridoma plaub qhov chaw yog raws li kev sib xyaw ntawm cov hlwb somatic los ntawm ob kab sib txawv hybridoma cell kab kom qhia cov nas IgG sib xws. Los ntawm kev siv tshuab genetic engineering, cov tshuaj tiv thaiv kab mob tuaj yeem hloov kho genetic los tsim cov tshuaj tiv thaiv kab mob bispecific. Ob lub tshuaj monoclonal sib txawv tau tsim, thiab cov khoom Fab lossis cov saw hlau hnyav thiab cov saw hlau sib txawv ntawm ob lub tshuaj tiv thaiv kab mob tau txiav sib cais. Los ntawm kev sib txuas sib txuas lossis cov thev naus laus zis sib xyaw ua ke, ob qho khoom tau sib xyaw ua ke los tsim cov tshuaj tiv thaiv kab mob bispecific. Txawm hais tias qhov nyuaj ntawm cov thev naus laus zis genetic engineering yog qhov siab heev, tam sim no nws yog txoj hauv kev siv ntau tshaj plaws rau kev tsim cov tshuaj tiv thaiv kab mob bispecific los kho cov qauv thiab kev ua haujlwm ntawm cov tshuaj tiv thaiv kab mob. Thaum ua qhov kev tsim cov tshuaj tiv thaiv kab mob bispecific, lub hauv paus ntsiab lus ntawm cov tshuaj tiv thaiv kab mob hla kev ua haujlwm yuav raug siv, tab sis cov tshuaj tiv thaiv kab mob hla kev ua haujlwm yuav ua rau muaj kev tsis sib haum xeeb, yog li nws yuav raug txiav txim siab zoo hauv kev siv cov qauv tsim cov tshuaj tiv thaiv kab mob bispecific, xws li kev kho mob bispecific antibody.

Kev Ntxuav Cov Tshuaj Antibody Bispecific

Kev ntxuav cov tshuaj tiv thaiv kab mob bispecific yog cov txheej txheem ntawm kev cais thiab ntxuav cov tshuaj tiv thaiv kab mob siab-purity. Ob txoj kev, centrifugation thiab kev lim dej tob, yog siv los tshem tawm qee cov khoom tsis huv uas yaj tau. Lub hom phiaj bispecific antibody yog thawj zaug ntes los ntawm affinity chromatography. Rau IgG zoo li bispecific antibodies, protein A affinity chromatography yog siv, thaum rau cov tshuaj tiv thaiv kab mob tsis zoo li IgG, lub teeb saw hlau raws li affinity chromatography tuaj yeem siv. Tom qab ntawd, cov tshuaj tiv thaiv kab mob raug incubated nyob rau hauv cov xwm txheej pH qis rau ib lub sijhawm, ua rau cov qauv protein ntawm qhov chaw ntawm lub hnab ntawv kab mob raug cuam tshuam, yog li poob lub peev xwm kis kab mob rau cov hlwb. Intermediate deep filtration yog ua kom tshem tawm cov khoom tsis huv xws li cov protein cell host (HCP). Kev huv ntawm cov tshuaj tiv thaiv kab mob tau txhim kho los ntawm cov txheej txheem xws li ion exchange chromatography, thiab txhua yam kab mob seem raug tshem tawm los ntawm nanofiltration lossis ultrafiltration techniques. Thaum kawg, cov qauv raug concentrated thiab hloov nrog lub buffer formulation tsim nyog.

Kev Ua Haujlwm Pabcuam Tsim Cov Tshuaj Tiv Thaiv Kab Mob Ob Hom

Cov kauj ruam	Cov Ntsiab Lus Kev Pabcuam	Lub Sijhawm
Kev Tsim Cov Gene	Tsim txog li 3 txoj kev tsim qauv sib txawv ntawm cov kab ke, de novo tiam ntawm recombinant antibody DNA plasmids	2-3 lub lis piam
Kev Ntsuas Me Me	Kev tsim cov protein antibody bispecific → Kev qhia me me hauv cov kab mob mammalian cell lines → Kev lees paub los ntawm SDS-PAGE → Kev tshuaj xyuas kev sib txuas nrog cov recombinant protein antigens los ntawm ELISA → Kev sib piv ntawm cov clones nrog cov niam txiv antibodies	5-6 lub lis piam
Txheeb xyuas	Kev qhia tawm ntawm ob lub tshuaj tiv thaiv kab mob bispecific thawj zaug → Kev tshuaj xyuas kev sib txuas los ntawm ELISA → Kev ntsuam xyuas ELISA ntawm cov tshuaj tiv thaiv kab mob uas cov tshuaj tiv thaiv nas pom tau	1 lub lim tiam
Kev Tsim Tshuaj Antibody	Kev tsim cov tshuaj tiv thaiv kab mob loj	3-4 lub lis piam

Peb Qhov Zoo

Peb npog cov kev pabcuam sab saud thiab sab hauv qab ntawm kev tsim cov tshuaj tiv thaiv kab mob, thiab tuaj yeem muab cov kev pabcuam kev tshaj lij los ntawm kev npaj cov tshuaj tiv thaiv kab mob, kev ntxuav cov tshuaj tiv thaiv kab mob bispecific thiab kev sib cais thiab kev ntxuav cov tshuaj tiv thaiv kab mob, kev txheeb xyuas cov tshuaj tiv thaiv kab mob, kev lees paub cov tshuaj tiv thaiv kab mob, thiab lwm yam, uas tuaj yeem siv rau kev kho cov tshuaj tiv thaiv kab mob bispecific.

Kev Qhia Siab

Kev qhia tawm cov protein ntau hauv cov hlwb CHO.
Kev Khaws Cia Ruaj Khov

>2 lub lis piam nyob rau hauv cov ntshav ntawm 37 ° C.
Tsis muaj teeb meem yaj

>30 mg/ml
Cov Cell Ruaj Khoov

Muaj ntau cov kab mob cell ruaj khov nrog cov antigens ntawm qog nqaij hlav tau tsim los xyuas kom meej ntau cov tshuaj tiv thaiv bispecific