Hybridoma Technology Platform
Alpha Lifetech muab cov neeg siv khoom nrog cov khoom siv antibody thiab cov khoom siv recombinant antibody zoo thiab cov kev pabcuam.
Thov Kev Hais Nqe01
Hybridoma Technology Platform
Alpha Lifetech muaj kev paub nplua nuj hauv kev tsim cov tshuaj tiv thaiv kab mob. Tau ntau xyoo, Alpha Lifetech tau tsim kom muaj lub platform tsim cov tshuaj tiv thaiv kab mob (xws li kev tsim cov tshuaj monoclonal antibody, kev tsim cov tshuaj polyclonal antibody, kev tsim cov tshuaj recombinant monoclonal antibody, thiab lwm yam kev pabcuam npaj thiab tsim cov tshuaj tiv thaiv kab mob).
Alpha Lifetech muab cov khoom siv thiab cov kev pabcuam zoo rau cov neeg siv khoom, thiab cov tshuaj tiv thaiv kab mob uas tau npaj tseg muaj qhov tshwj xeeb thiab ruaj khov zoo. Ntau yam cuab yeej ntxuav cov tshuaj tiv thaiv kab mob thiab cov khoom siv tuaj yeem siv los muab cov kev pabcuam ntxuav cov tshuaj tiv thaiv kab mob los ntawm ntau qhov chaw xws li luav, yaj, qaib, nas monoclonal antibodies, nrog rau cov kev pabcuam ntxuav protein A/G affinity thiab cov kev pabcuam sib cais cov tshuaj tiv thaiv kab mob.
Raws li ntau yam txheej txheem npaj cov tshuaj tiv thaiv kab mob xws li hybridoma cell technology, Alpha Lifetech tuaj yeem muab cov kev pabcuam tshwj xeeb rau monoclonal thiab polyclonal antibodies ntawm ntau hom. Tsis tas li ntawd, ntau yam kev pabcuam los ntawm kev ua tiav siab, kev npaj cov tshuaj tiv thaiv kab mob, kev ntxuav cov tshuaj tiv thaiv kab mob mus rau kev lees paub cov tshuaj tiv thaiv kab mob. Peb yuav xaiv txoj kev ntxuav raws li lub hom phiaj tshwj xeeb ntawm tus neeg siv khoom. Raws li cov txheej txheem platform dav dav xws li antibody development platform thiab protein platform, Alpha Lifetech npog cov kev pabcuam upstream thiab downstream ntawm kev tsim cov tshuaj tiv thaiv kab mob, muab kev ntxuav cov tshuaj tiv thaiv kab mob, antibody high throughput sequencing, antibody validation thiab lwm yam kev pabcuam kev txawj.
Kev Siv Tshuab Hybridoma
Cov thev naus laus zis Hybridoma siv tau los npaj cov tshuaj monoclonal antibodies. Los ntawm kev sib xyaw cov hlwb spleen los ntawm cov nas uas tau txhaj tshuaj tiv thaiv kab mob nrog cov hlwb myeloma los ntawm tib neeg lossis nas, cov hlwb hybridoma raug tsim thiab tso tawm cov tshuaj tshwj xeeb. Cov tshuaj monoclonal antibodies uas tsim los ntawm nas tuaj yeem siv rau hauv kev tshawb fawb xws li enzyme-linked immunosorbent assay, radioimmunoassay, immunohistochemistry, flow cytometry, protein purification, thiab lwm yam. Cov qauv monoclonal antibody suav nrog ascites, hybridoma supernatant, thiab lwm yam. Cov qauv tau los ntawm kev txhaj cov hlwb hybridoma rau hauv lub plab ntawm cov tsiaj thiab siv qhov chaw ib puag ncig ntawm lub plab ntawm tus tsiaj los tsim cov tshuaj tiv thaiv hu ua ascites sample. Cov hlwb hybridoma txhaj rau hauv lub plab ntawm cov tsiaj tuaj yeem loj hlob thiab tso tawm cov tshuaj monoclonal antibodies, uas tuaj yeem sib sau ua ke hauv ascites. Vim muaj qhov ua tau ntawm cov khoom tsis huv (cov protein tsiaj, cov tshuaj hormones, thiab lwm yam) hauv cov tshuaj no, kev ntxuav yog qhov yuav tsum tau ua. Raws li kev ua haujlwm ntawm B lymphocytes, monoclonal antibodies feem ntau raug tso tawm rau hauv cov kab lis kev cai thaum cog cov hlwb hybridoma. Cov kua uas muaj cov monoclonal antibodies hauv cov kab lis kev cai hu ua hybridoma supernatant. Qhov huv ntawm cov monoclonal antibodies hauv supernatant yog qhov siab heev, tab sis yuav tsum tau ua kom huv ntxiv rau kev npaj loj vim tias lawv tau txais los ntawm kev cog qoob loo hauv vitro.
Kev ntxuav cov tshuaj tua kab mob tuaj yeem cais cov tshuaj tua kab mob uas muaj cov khoom dawb huv siab los ntawm cov complexes. Tam sim no, kev ntxuav cov tshuaj tua kab mob thiab kev sib cais thiab kev ntxuav cov tshuaj tua kab mob feem ntau yog cov txheej txheem ntxuav cov tshuaj tua kab mob, feem ntau suav nrog kev ntxuav protein A/G, ion exchange chromatography, gel filtration, precipitation thiab hydrophobic interaction chromatography. Ntawm lawv, kev pabcuam ntxuav cov protein A/G affinity yog nrov heev hauv ntau yam kev siv ntxuav cov tshuaj tua kab mob. Cov txheej txheem ntawm Alpha Lifetech npaj cov tshuaj monoclonal raws li cov thev naus laus zis hybridoma tau pom hauv Daim Duab 1.
Daim Duab 1 Daim duab qhia txog cov txheej txheem rau kev npaj cov tshuaj monoclonal antibodies raws li kev siv tshuab hybridoma.
Kev Siv Tshuaj Antibody Conjugate thiab Hybridoma Technology
Cov tshuaj tiv thaiv kab mob sib xyaw ua ke (ADC) yog ib yam khoom siv tshiab uas sib xyaw cov tshuaj tiv thaiv kab mob nrog cov tshuaj cytotoxic. Cov tshuaj tiv thaiv kab mob sib xyaw ua ke lub peev xwm paub txog cov tshuaj tiv thaiv kab mob nrog cov nyhuv tua kab mob muaj zog ntawm cov tshuaj cytotoxic, thiab xa cov tshuaj cytotoxic mus rau hauv cov hlwb qog nqaij hlav los ntawm cov tshuaj tiv thaiv kab mob, yog li ua tiav kev kho mob kom raug. Feem ntawm cov tshuaj tiv thaiv kab mob hauv cov tshuaj ADC feem ntau yog npaj los ntawm kev siv tshuab hybridoma lossis lwm yam kev tsim cov tshuaj tiv thaiv kab mob, xws li kev siv tshuab genetic engineering antibody. Kev siv tshuab Hybridoma muab cov khoom siv tshuaj tiv thaiv kab mob zoo rau cov tshuaj ADC. Cov tshuaj ADC thiab cov tshuaj monoclonal uas tau npaj los ntawm kev siv tshuab hybridoma ua lub luag haujlwm tseem ceeb hauv cov teb xws li kev kho mob qog noj ntshav. Lawv tuaj yeem siv ib leeg lossis ua ke los txhim kho kev kho mob zoo.
Cov tshuaj monoclonal antibodies uas tau npaj los ntawm thev naus laus zis hybridoma muaj qhov tshwj xeeb siab, tuaj yeem khi thiab paub cov antigens tshwj xeeb, thiab tuaj yeem tsim tawm ntawm qhov loj. Los ntawm thev naus laus zis hybridoma sequencing, cov noob antibody hauv cov hlwb hybridoma tuaj yeem rho tawm thiab sequenced kom tau txais cov kab ke sib xws, uas yog qhov zoo rau kev qhia tawm antibody tom qab. Cov tshuaj monoclonal antibodies uas tau npaj los ntawm thev naus laus zis hybridoma tuaj yeem siv los ntes cov cim kab mob tshwj xeeb, xws li cov cim qog nqaij hlav, cov antigens viral, thiab lwm yam, muab kev txhawb nqa zoo rau kev kuaj mob thaum ntxov ntawm cov kab mob. Tsis tas li ntawd, nws kuj tseem tuaj yeem siv rau hauv kev sim xws li immunoblotting thiab immunohistochemistry los kawm txog kev qhia tawm protein, qhov chaw, cov qauv, thiab kev ua haujlwm.
Kev siv tshuab hybridoma tsim cov tshuaj tua kab mob ua haujlwm
| Cov kauj ruam | Cov Ntsiab Lus Kev Pabcuam | Lub Sijhawm |
|---|---|---|
| Kauj Ruam 1: Kev txhaj tshuaj tiv thaiv kab mob rau tsiaj | (1) Txhaj tshuaj tiv thaiv kab mob rau tsiaj 4 zaug, txhaj tshuaj ntxiv 1 koob, tag nrho 5 koob tau txhaj tshuaj lawm. (2) Cov ntshav tsis zoo ua ntej txhaj tshuaj tiv thaiv kab mob tau sau, thiab ELISA tau ua rau koob tshuaj thib plaub los ntes cov ntshav titer. (3) Yog tias cov tshuaj tiv thaiv kab mob hauv cov ntshav ntawm koob tshuaj thib plaub ua tau raws li qhov yuav tsum tau ua, yuav muab ib koob tshuaj ntxiv rau 7 hnub ua ntej sau ntshav. Yog tias nws tsis ua tau raws li qhov yuav tsum tau ua, kev txhaj tshuaj tsis tu ncua yuav txuas ntxiv mus. (4) Muaj peev xwm ua tau zoo, cov hlwb Spleen raug rho tawm rau kev sib xyaw ua ke ntawm tes. | 10-12 lub lis piam |
| Kauj Ruam 2: Kev Sib Koom Ua Ke thiab Kev Tshuaj Xyuas | (1) Kev Sib Koom Ua Ke: Tom qab txhaj tshuaj tiv thaiv kab mob, cov hlwb ntawm nas tau sau thiab sib koom ua ke nrog cov hlwb myeloma. (2) Kev nthuav dav thiab kev tshuaj ntsuam: Cov hlwb hybridoma tau txais tau raug rau kev nthuav dav thiab kev tshuaj ntsuam ELISA kom tau txais cov clones ua haujlwm tau zoo heev. | 6 lub lis piam |
| Kauj Ruam 3: Subclone | Subcloning 15-25 lub hlwb niam zoo siv txoj kev txwv dilution, thiab thaum kawg txiav txim siab 10-15 lub clones zoo | 3-4 lub lis piam |
| Kauj Ruam 4: Kev Tsim Ascites thiab Kev Ntxuav Antibody | (1) Kev tsim cov ascites: 10-15 hom hybridoma siv rau kev tsim cov ascites hauv nas (yog tias tau txais ntau hom hybridoma, lawv yuav raug xa mus rau hauv daim ntawv cellular) (2) Kev txheeb xyuas Ascites: Ascites ELISA nrhiav pom, muaj zog (protein/virus>10 ^ 5, me me molecule/peptide>10 ^ 4) rau kev ntxuav downstream (3) Kev Ntxuav Cov Protein A/G: Kev Ntxuav Cov Ascites | 3-4 lub lis piam |
| Kauj Ruam 5: Kev Xa Khoom | (1) Muab 3-5 hom monoclonal antibody hybridoma. (2) Txhua lub cell hybridoma muab 1mg ntawm cov tshuaj tua kab mob uas tau ntxuav (ascites purification). (3) Daim Ntawv Qhia Txog Kev Sim | 1 lub lim tiam |
Ntau Hom
Kev tiv thaiv kab mob tuaj yeem muab rau ntau yam tsiaj txhu, suav nrog tab sis tsis txwv rau nas, luav, yaj, thiab lwm yam.
Lub Hom Phiaj Ntau Yam
Raws li thev naus laus zis hybridoma, muaj ntau yam kev pabcuam nrhiav cov tshuaj tiv thaiv kab mob: cov protein, peptides, cov molecule me me, cov kab mob, cov protein membrane, mRNA, thiab lwm yam.
Cov Ntaub Ntawv Txog Kev Taug Qab
Cov qauv tswj xyuas zoo ntawm QC (lub zog tiv thaiv kab mob, kev tswj xyuas zoo ntawm PBMC, kev tswj xyuas zoo ntawm tsev qiv ntawv, thiab kev tswj xyuas zoo ntawm kev tshuaj ntsuam xyuas), cov ntawv qhia txog kev sim ua lus Askiv, cov ntaub ntawv sim thawj
Kev Pabcuam Rau Tus Kheej
Kev kho kom haum rau ib qho kev pab cuam kom tau raws li qhov xav tau ntawm ntau tus neeg siv khoom
Kev Pabcuam Tsim Tshuaj Monoclonal Antibody
Peb tuaj yeem muab cov kev pabcuam tsim cov tshuaj monoclonal antibody zoo, huv si, thiab tshwj xeeb heev, suav nrog kev tsim cov tshuaj monoclonal antibodies nas thiab cov tshuaj monoclonal antibodies luav.
Hybridoma Technology Platform
Xws li kev pab cuam txhaj tshuaj tiv thaiv kab mob, kev pab npaj cov tshuaj tiv thaiv kab mob, kev ntxuav cov tshuaj tiv thaiv kab mob, kev txheeb xyuas cov tshuaj tiv thaiv kab mob siab, kev lees paub cov tshuaj tiv thaiv kab mob, thiab lwm yam
Ib Lub Tshuab Sorting B Cell
Alpha Lifetech muaj qhov zoo hauv lub sijhawm tshuaj ntsuam thiab tau txais cov tshuaj tiv thaiv zoo. Nws tuaj yeem muab kev tsim cov tshuaj antigen, kev tsim khoom, thiab kev hloov kho, kev tiv thaiv tsiaj, kev tshuaj ntsuam xyuas cov hlwb B ib leeg, kev txheeb xyuas cov hlwb ib leeg.
Phage Display Antibody Development Platform
Alpha Lifetech tuaj yeem muab cov kev pabcuam tsim cov tshuaj tiv thaiv kab mob phage los ntawm kev npaj cov tshuaj tiv thaiv kab mob, kev ntxuav cov tshuaj tiv thaiv kab mob, kev txheeb xyuas cov tshuaj tiv thaiv kab mob, thiab lwm yam.
Cov Kev Pab Cuam Txog Kev Siv Tshuab
Kev Taw Qhia Txog Kev Siv Tshuab HybridomaVIDEO
Yog tias koj muaj lus nug, thov koj xav tiv tauj peb txhua lub sijhawm.
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2018-07-16 
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