Kev Pabcuam Purification Protein Membrane

Alpha Lifetech tau ua tiav npaj ntau txhiab tus recombinant proteins rau peb cov neeg siv khoom, suav nrog recombinant antibodies, secreted proteins, transmembrane proteins, proteases, thiab lwm yam, nrog rau cov nqi ua tiav siab. Cov neeg siv khoom tsuas yog yuav tsum muab cov protein sequence, CDS lossis protein lub npe, thiab Alpha Lifetech tuaj yeem tsim cov fusion protein expression thiab purification plan tiav hauv lub sijhawm luv dua. Peb tuaj yeem muab cov kev pabcuam protein expression raws li plaub lub tshuab expression tseem ceeb, thiab xaiv cov fusion protein expression thiab purification strategy sib txawv raws li cov neeg siv khoom xav tau.

Alpha Lifetech muaj ntau yam cuab yeej thiab khoom siv ntxuav protein, uas tuaj yeem muab cov kev pabcuam ntxuav protein ntuj thiab cov kev pabcuam ntxuav protein recombinant, xws li affinity purification, molecular sieve chromatography, ion exchange chromatography, thiab hydrophobic chromatography. Peb yuav xaiv cov txheej txheem ntxuav raws li qhov xav tau tshwj xeeb ntawm peb cov neeg siv khoom, thiab muab lawv ua ke nrog peb lub platform ntxuav protein kom tau txais cov khoom protein zoo hauv lub sijhawm luv luv.

Kev faib tawm ntawm cov protein membrane

Raws li kev faib tawm ntawm cov protein hauv daim nyias nyias thiab kev sib raug zoo ntawm cov protein membrane thiab cov lipids membrane, cov protein membrane raug muab faib ua peb pawg, suav nrog cov protein peripheral membrane, cov protein integrated membrane, thiab cov protein lipid anchoring.

Cov protein channel ionic

Ionic channel protein yog ib qho protein membrane uas sib xyaw ua ke uas nkag mus rau hauv cov biological membranes los tsim cov qauv channel tshwj xeeb, xaiv cov ions kom raug thauj mus los hla cov membranes sab hauv thiab sab nraud ntawm lub cell, thaum tiv thaiv lwm cov ions kom tsis txhob hla dhau. Kev xaiv ntawm cov ion channel proteins yog qhov tseem ceeb rau kev tswj hwm lub neej ntawm lub cell xws li qhov sib txawv ntawm ion concentration ntawm sab hauv thiab sab nraud ntawm lub cell, neurotransmitter thauj, thiab lwm yam.

Cov receptors sib txuas nrog G protein

Cov G protein txuas nrog receptors yog ib lo lus dav dav rau ib pawg loj ntawm cov membrane protein receptors, uas yog cov cell surface receptors ntau tshaj plaws hauv eukaryotes. Lub luag haujlwm tseem ceeb ntawm G protein txuas nrog receptors yog xa cov ntaub ntawv extracellular mus rau sab hauv ntawm cov cell los ntawm kev sib cuam tshuam nrog G protein, ua lub luag haujlwm tseem ceeb hauv kev xa cov teeb liab. G protein txuas nrog receptors paub thiab khi rau extracellular ligands los ua rau cov kab ke intracellular signaling, yog li tswj ntau yam kev ua ub no ntawm cov cell.

Cov khoom me me zoo li tus kab mob

Ib yam khoom me me zoo li tus kab mob yog ib yam khoom me me uas tsim los ntawm kev sib sau ua ke ntawm ib lossis ntau yam protein ntawm tus kab mob. Cov khoom me me no zoo ib yam li cov khoom me me ntawm tus kab mob ntuj, tab sis tsis muaj cov khoom siv caj ces ntawm tus kab mob, yog li ntawd lawv tsis kis tau.

Cov Khoom Me Me Zoo Li Kab Mob tuaj yeem ua lub platform tso saib rau cov protein membrane, ua piv txwv txog kev hloov pauv thiab kev ua haujlwm ntawm cov protein membrane hauv cov chaw ib puag ncig los ntawm kev tso saib cov protein membrane tshwj xeeb ntawm Cov Khoom Me Me Zoo Li Kab Mob. Hauv kev tsim tshuaj tiv thaiv kab mob, cov antigens tiv thaiv ntawm tus kab mob, feem ntau yog cov protein membrane, tuaj yeem tso saib rau ntawm Cov Khoom Me Me Zoo Li Kab Mob los txhawb lub cev kom tsim cov lus teb tiv thaiv kab mob tiv thaiv cov antigens no. Cov tshuaj tiv thaiv kab mob uas npaj los ntawm txoj kev no muaj kev nyab xeeb heev thiab ua rau lub cev tiv thaiv kab mob.

Kev Ntxuav Cov Protein Membrane

Muaj ntau txoj kev rau kev ntxuav cov protein membrane, ntawm cov uas affinity chromatography siv qhov affinity ntawm cov protein membrane lawv tus kheej lossis fusion tags, xws li His tags, nrog lawv cov ligands tshwj xeeb kom ua tiav cov protein membrane purification thiab sib cais. Ion exchange chromatography siv cov khoom them ntawm cov protein membrane los cais lawv ntawm ion exchange resins. Txawm li cas los xij, cov kua protein membrane feem ntau muaj cov tshuaj ntxuav tes, thiab kev tawm haujlwm yuav cuam tshuam rau qhov ua tau zoo ntawm ion exchange chromatography. Gel filtration chromatography yog siv qhov sib txawv ntawm cov protein membrane molecular qhov hnyav los cais ntawm gel kem, los ntawm txoj kev no, peb tuaj yeem tau txais cov protein membrane purity siab. Vim yog qhov affinity thiab hydrophobicity ntawm cov protein membrane, lawv tuaj yeem sib cais ntawm chromatographic kem. Txoj kev no ua haujlwm tau zoo, ceev, thiab rhiab heev, tab sis tus nqi kuj tseem siab.

Kev ntxuav cov protein membrane-Alpha Lifetech

Daim Duab 1 Daim duab qhia txog kev ntxuav cov protein affinity

Cov txiaj ntsig ntawm kev ntxuav cov protein membraneVIM LI CAS ho xaiv

Kev ua kom zoo dua ntawm codon

Kev qhia tawm cov protein uas yaj tau zoo thiab kev ua haujlwm ntawm cov protein ntau tuaj yeem lav tau

Ntau hom kev ua kom cov protein fermentation: me me (1L, 10L, thiab 30L), loj (80L, 130L, 250L, thiab 500L fermentation tanks)

Kev npaj cov protein recombinant purity siab hauv milligrams thiab grams hauv lub sijhawm luv luv

Endotoxin tsawg: LAL nrhiav txoj kev

Ib qho kev txhawb nqa cov ntaub ntawv GMP ua tiav ua kom ntseeg tau tias muaj kev taug qab ntawm txhua yam khoom siv, cov tshuaj reagents, thiab cov ntaub ntawv npaj rau cov khoom.

Rov qab mus rau nplooj ntawv Membrane Protein Platform

Yog tias koj muaj lus nug, thov koj xav tiv tauj peb txhua lub sijhawm.

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Kev Pabcuam Tshwj Xeeb

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