Phaj Display Antibody Development Platform

Alpha Lifetech, nrog rau nws qhov kev paub dav dav hauv kev tshawb pom cov tshuaj tiv thaiv kab mob, tuaj yeem muab cov kev pabcuam monoclonal thiab polyclonal antibody rau ntau hom, nrog rau kev tsim kho cov tsev qiv ntawv tshuaj tiv thaiv thiab tshuaj ntsuam xyuas. Alpha Lifetech tuaj yeem muab cov neeg siv khoom nrog cov tshuaj tiv thaiv zoo thiab cov khoom siv tshuaj tiv thaiv kab mob sib xyaw ua ke, nrog rau cov kev pabcuam cuam tshuam, los npaj cov tshuaj tiv thaiv zoo, tshwj xeeb, thiab ruaj khov. Los ntawm kev siv cov antibody platforms thiab antibody engineering, peb muab cov kev pab cuam npog cov dej ntws thiab nqes dej ntawm cov tshuaj tiv thaiv kab mob ntau lawm, suav nrog cov kev pab cuam xws li kev npaj tshuaj tiv thaiv kab mob, purification, antibody sequencing, thiab validation.

Alpha Lifetech muaj qhov paub tab antibody nrhiav pom platform uas muab cov neeg siv khoom nrog ntau yam kev pabcuam suav nrog kev tiv thaiv tsiaj, phage zaub antibody tsev qiv ntawv tsim thiab tshuaj ntsuam, antibody sequencing, antibody expression, antibody purification, antibody validation, and antibody labeling based on hybridoma technology, single B cell technology, phage display technology, thiab ntau dua. Alpha Lifetech muaj cov txheej txheem tsim khoom, thiab ntxiv rau cov kev pabcuam tshuaj tiv thaiv kab mob, txhawb kev pabcuam xws li tshuaj tiv thaiv tib neeg, kev tiv thaiv kev sib raug zoo ntawm lub cev, ADC tshuaj tsim thiab kev tsim kho, thiab CAR-T tom qab tsim qauv kuj tuaj yeem muab. Nyob rau tib lub sijhawm, Alpha Lifetech tau tsim lub tsev qiv ntawv tshuaj tiv thaiv kab mob raws li M13, T4, thiab T7 phage cov cuab yeej siv thev naus laus zis, nrog lub peev xwm khaws cia txog li 10^8-10^9. Tus nqi zoo, qhov nkag, thiab ntau haiv neeg ntawm lub tsev qiv ntawv tuaj yeem ncav cuag ntau dua 90%.

Phaj zaub Technology

Monoclonal antibodies ntau lawm tau pib npaj siv hybridoma monoclonal antibody technology los tsim nas hybridoma monoclonal antibody. Tshwj xeeb, los ntawm kev sib xyaw cov hlwb spleen los ntawm cov nas txhaj tshuaj nrog myeloma hlwb los ntawm tib neeg lossis nas, hybridoma hlwb raug tsim, uas tso cai rau cov hybridoma monoclonal antibody antibodies. Los ntawm tib neeg kev hloov kho ntawm nas monoclonal antibodies thiab genetic engineering hloov kho ntawm cov tshuaj tiv thaiv kom muab lawv ib tug tas li tib neeg immunoglobulin cheeb tsam, lawv immunogenicity yuav txo tau. Monoclonal antibody engineering yog siv dav hauv kev siv tshuaj biomedical thiab kev kho mob.

Alpha Lifetech tuaj yeem muab cov kev pabcuam ib-nres antibody engineering los ntawm preclinical research protocol design to antibody drug conjugates (ADCs) discovery and animal validation. Antibody tshuaj conjugates (ADCs) tuaj yeem xa cov tshuaj khomob rau cov qog nqaij hlav cancer. Tom qab khi rau cov hom phiaj tshwj xeeb uas qhia txog cov qog nqaij hlav cancer, ADC tso cov tshuaj cytotoxic rau hauv cov qog nqaij hlav cancer. Alpha Lifetech tseem tuaj yeem muab cov neeg siv khoom nrog cov kev pabcuam antibody affinity maturation. Nrog rau kev hloov pauv zoo tshaj plaws thiab kev siv tshuab thev naus laus zis thev naus laus zis thev naus laus zis, cov tshuaj tiv thaiv kab mob nrog qee qhov kev sib raug zoo yog thawj zaug kuaj, thiab tom qab ntawd cov amino acid hloov pauv tau qhia los ua kom muaj ntau yam sib txawv. Tom qab ntawd, cov tshuaj tiv thaiv kab mob siab affinity tau raug tshuaj xyuas thiab tshuaj xyuas siv cov txheej txheem tshuaj ntsuam. Tom qab ntau qhov kev ua kom zoo dua qub thiab kev txheeb xyuas cov qauv, cov tshuaj tiv thaiv nrog ntau dua affinity thiab muaj zog tshwj xeeb thaum kawg tau txais.

Alpha Lifetech tuaj yeem tsim ntau hom tshuaj tiv thaiv kab mob phage cov tsev qiv ntawv, suav nrog cov tsev qiv ntawv tiv thaiv kab mob, cov tsev qiv ntawv ib txwm muaj, cov tsev qiv ntawv semi hluavtaws, thiab cov tsev qiv ntawv hluavtaws. Raws li lub peev xwm siab ntawm cov tsev qiv ntawv tshuaj tiv thaiv kab mob, cov tshuaj tiv thaiv tshwj xeeb tuaj yeem tau txais. Ntau phagemid vectors xws li pMECS, pComb3X, thiab pCANTAB 5E tuaj yeem muab tau, nrog rau cov kab mob xws li TG1 E. coli, XL1 Blue, thiab ER2738. Ntxiv nrog rau lub tsev qiv ntawv muaj peev xwm txog li 10^9, lub hom phiaj tawg ntu ntawm lub tsev qiv ntawv kuj tseem siab, tsim kom muaj cov xwm txheej txaus rau kev tshuaj xyuas cov tshuaj tiv thaiv uas txaus siab rau cov neeg siv khoom. Daim ntawv qhia txog kev ua haujlwm ntawm Alpha Lifetech qhov kev npaj tshuaj tiv thaiv kab mob raws li phage zaub thev naus laus zis tau qhia hauv daim duab 1.

Fig.1 Daim duab txheej txheem rau kev npaj cov tshuaj tiv thaiv kab mob monoclonal raws li phage zaub technology.

Phaj Display Antibody Production Workflow

Cov kauj ruam	Cov ntsiab lus kev pabcuam	Sijhawm
Kauj Ruam 1: Kev txhaj tshuaj rau tsiaj	(1) Tsiaj txhaj tshuaj 4 zaug, txhaj tshuaj tiv thaiv kab mob 1 koob, tag nrho ntawm 5 koob txhaj tshuaj. (2) Cov tshuaj tsis zoo ua ntej sau cov tshuaj tiv thaiv kab mob, thiab ELISA tau ua ntawm plaub koob tshuaj kom pom cov ntshav titer. (3) Yog tias cov tshuaj tiv thaiv kab mob ntshav qab zib thib plaub ua tau raws li qhov yuav tsum tau ua, ib qho kev txhaj tshuaj ntxiv yuav raug muab 7 hnub ua ntej sau ntshav. Yog tias nws tsis ua raws li qhov yuav tsum tau ua, kev txhaj tshuaj niaj hnub yuav txuas ntxiv mus. (4) Tsim nyog muaj peev xwm, sau ntshav thiab sib cais ntawm monocytes	10 lub lis piam
Kauj ruam 2: Kev npaj cDNA	(1) PBMC Tag Nrho RNA Extraction (RNA Extraction Kit) (2) High fidelity RT-PCR npaj ntawm cDNA (reverse transcription kit)	1 hnub
Kauj Ruam 3: Kev tsim kho ntawm Antibody Library	(1) Siv cDNA ua tus qauv, cov noob tau nthuav dav los ntawm ob puag ncig ntawm PCR. (2) Phage kev tsim kho thiab kev hloov pauv: gene splicing phagemid vector, electroporation transformation ntawm TG1 tus tswv tsev cov kab mob, kev tsim kho cov tsev qiv ntawv antibody. (3) Kev Taw Qhia: Random xaiv 24 clones, PCR txheeb xyuas tus nqi zoo + tus nqi nkag. (4) Kev npaj phage pab: M13 phage amplification + purification. (5) Phaj tso saib tsev qiv ntawv cawm	3-4 lub lis piam
Kauj Ruam 4: Antibody Library Screening (3 rounds)	(1) Default 3-round screening (khoom-theem kev tshuaj ntsuam): kev ntsuam xyuas siab kom tshem tawm cov tshuaj tiv thaiv uas tsis yog tshwj xeeb rau qhov siab tshaj plaws. (2) Ib leeg clone amplification bacteriophage xaiv + IPTG induced qhia + ELISA nrhiav pom zoo clones. (3) Tag nrho cov clones zoo raug xaiv rau kev sib txuas ntawm cov noob.	4-5 lub lis piam

Qhov zoo ntawm phage Display Technology

Alpha Lifetech muaj kev paub nplua nuj hauv kev txhim kho cov tshuaj tiv thaiv kab mob. Tau ntau xyoo, Alpha Lifetech tau tsim kom muaj kev txhim kho cov tshuaj tiv thaiv kab mob.

Kev pab txhawb nqa

Peb tuaj yeem muab cov tsiaj sib txawv raws li kev tiv thaiv lub tsev qiv ntawv kev tsim kho thiab cov khoom siv tshuaj tiv thaiv lub tsev qiv ntawv tshuaj ntsuam xyuas raws li cov neeg siv khoom xav tau

Ntau Lub Hom Phiaj

Muaj ntau lub hom phiaj antibody nrhiav tau cov kev pab cuam: proteins, peptides, me me molecules, kab mob, membrane proteins, mRNA, thiab lwm yam.

Ntau Vectors

Kev tsim kho tsev qiv ntawv tus kheej, peb tuaj yeem muab ntau yam bacteriophage vectors nrog rau PMECS, pComb3X, thiab pCANTAB 5E, thiab hloov kho raws li cov neeg siv khoom xav tau

Mature Platform

Lub peev xwm khaws cia tuaj yeem ncav cuag 10 ^ 8-10 ^ 9, cov nqi nkag yog tag nrho siab dua 90%, thiab kev sib raug zoo ntawm cov tshuaj tiv thaiv tau los ntawm kev tshuaj ntsuam feem ntau yog nyob rau theem nM pM.

Kev Pabcuam ntsig txog

Ntau hom kev txhim kho Antibody

Monoclonal Antibody Development Service

Hybridoma Technology Platform

Tib B Cell Sorting Platform

Phaj Display Antibody Development Platform

Kev txhim kho monoclonal antibody-Alpha Lifetech

Monoclonal Antibody Development Service

Peb tuaj yeem muab cov kev pabcuam zoo, siab dawb huv, thiab tshwj xeeb monoclonal antibody kev pabcuam, suav nrog kev tsim cov nas monoclonal antibodies thiab luav monoclonal antibodies.

Hybridoma Technology Platform

Xws li kev txhaj tshuaj tiv thaiv kab mob, kev pabcuam tshuaj tiv thaiv kab mob, tshuaj tua kab mob purification, tshuaj tiv thaiv kab mob siab dhau los ua ke, tshuaj tiv thaiv kab mob, thiab lwm yam

Tib B Cell Sorting Platform

Alpha Lifetech muaj qhov zoo hauv lub sijhawm tshuaj ntsuam thiab tau txais cov tshuaj tiv thaiv zoo. Nws tuaj yeem muab cov qauv tsim antigen, synthesis, thiab kev hloov kho, kev tiv thaiv tsiaj txhu, kev tshuaj ntsuam xyuas B cell ib leeg, ib leeg cell sequencing.