Phage Display Antibody Development Platform

Alpha Lifetech, nrog nws cov kev paub dhau los hauv kev tshawb pom cov tshuaj tiv thaiv kab mob, tuaj yeem muab cov kev pabcuam monoclonal thiab polyclonal antibody rau ntau hom tsiaj, nrog rau kev tsim cov tsev qiv ntawv antibody thiab kev tshuaj ntsuam xyuas. Alpha Lifetech tuaj yeem muab cov neeg siv khoom nrog cov khoom antibody thiab recombinant antibody zoo, nrog rau cov kev pabcuam sib xws, los npaj cov tshuaj tiv thaiv zoo, tshwj xeeb heev, thiab ruaj khov. Los ntawm kev siv cov platform antibody thiab antibody engineering, peb muab cov kev pabcuam uas npog cov khoom siv antibody thiab cov khoom siv antibody, suav nrog cov kev pabcuam xws li kev npaj antibody, kev ntxuav, kev txheeb xyuas antibody, thiab kev lees paub.

Alpha Lifetech muaj lub platform tshawb pom cov tshuaj tiv thaiv kab mob uas laus lawm uas muab rau cov neeg siv khoom nrog ntau yam kev pabcuam suav nrog kev tiv thaiv kab mob ntawm tsiaj, kev tsim thiab tshuaj ntsuam cov tshuaj tiv thaiv kab mob phage, kev txheeb xyuas cov tshuaj tiv thaiv kab mob, kev qhia cov tshuaj tiv thaiv kab mob, kev ntxuav cov tshuaj tiv thaiv kab mob, kev lees paub cov tshuaj tiv thaiv kab mob, thiab kev sau cov tshuaj tiv thaiv kab mob raws li kev siv tshuab hybridoma, kev siv tshuab B cell ib leeg, kev siv tshuab phage display, thiab ntau ntxiv. Alpha Lifetech muaj cov kab ntau lawm txheem, thiab ntxiv rau cov kev pabcuam npaj cov tshuaj tiv thaiv kab mob, cov kev pabcuam txhawb nqa xws li kev ua kom cov tshuaj tiv thaiv kab mob ua neeg, kev loj hlob ntawm cov tshuaj tiv thaiv kab mob, kev tsim thiab kev txhim kho tshuaj ADC, thiab kev tsim cov kab ke CAR-T tom qab kuj tseem tuaj yeem muab tau. Tib lub sijhawm, Alpha Lifetech tau tsim lub tsev qiv ntawv tiv thaiv kab mob raws li M13, T4, thiab T7 phage display technology platforms, nrog rau lub peev xwm khaws cia txog li 10^8-10^9. Tus nqi zoo, tus nqi ntxig, thiab ntau haiv neeg ntawm lub tsev qiv ntawv tuaj yeem ncav cuag ntau dua 90%.

Phage zaub Technology

Kev tsim cov tshuaj monoclonal antibodies tau npaj ua ntej siv cov thev naus laus zis hybridoma monoclonal antibody los tsim cov nas hybridoma monoclonal antibody. Tshwj xeeb, los ntawm kev sib xyaw cov hlwb spleen los ntawm cov nas uas tau txhaj tshuaj tiv thaiv kab mob nrog cov hlwb myeloma los ntawm tib neeg lossis nas, cov hlwb hybridoma tau tsim, uas tso tawm cov tshuaj hybridoma monoclonal antibody tshwj xeeb. Los ntawm kev hloov kho tib neeg ntawm cov nas monoclonal antibodies thiab kev hloov kho genetic engineering ntawm cov tshuaj kom muab rau lawv thaj chaw immunoglobulin tib neeg tas li, lawv cov immunogenicity tuaj yeem txo qis. Monoclonal antibody engineering tau siv dav hauv kev siv biomedical thiab kev kho mob.

Alpha Lifetech tuaj yeem muab cov kev pabcuam tsim tshuaj tiv thaiv kab mob ib zaug xwb los ntawm kev tsim cov txheej txheem tshawb fawb ua ntej mus rau kev tshawb pom cov tshuaj tiv thaiv kab mob (ADCs) thiab kev lees paub tsiaj. Cov tshuaj tiv thaiv kab mob (ADCs) tuaj yeem xa cov tshuaj chemotherapy mus rau cov hlwb qog noj ntshav. Tom qab khi rau cov hom phiaj tshwj xeeb uas tau qhia rau ntawm cov hlwb qog noj ntshav, ADC tso cov tshuaj cytotoxic rau hauv cov hlwb qog noj ntshav. Alpha Lifetech kuj tseem tuaj yeem muab cov neeg siv khoom nrog cov kev pabcuam loj hlob ntawm cov tshuaj tiv thaiv kab mob. Nrog rau kev ua kom zoo dua ntawm kev hloov pauv thiab cov thev naus laus zis tshuaj ntsuam xyuas phage siab, cov tshuaj tiv thaiv kab mob uas muaj qee qhov sib xws raug tshuaj xyuas ua ntej, thiab tom qab ntawd cov kev hloov pauv amino acid raug qhia los tsim ntau yam sib txawv. Tom qab ntawd, cov tshuaj tiv thaiv kab mob siab tau raug soj ntsuam thiab tshuaj xyuas siv cov txheej txheem tshuaj ntsuam xyuas. Tom qab ntau qhov kev ua kom zoo dua thiab kev tshuaj xyuas cov qauv, cov tshuaj tiv thaiv kab mob uas muaj kev sib raug zoo dua thiab muaj zog dua tau txais thaum kawg.

Alpha Lifetech tuaj yeem tsim ntau hom kev tso saib cov phage antibody, suav nrog cov tsev qiv ntawv tiv thaiv kab mob, cov tsev qiv ntawv hauv zos, cov tsev qiv ntawv semi synthetic, thiab cov tsev qiv ntawv synthetic. Raws li lub peev xwm siab ntawm cov tsev qiv ntawv antibody, cov tshuaj tiv thaiv tshwj xeeb heev tuaj yeem tau txais. Ntau cov phagemid vectors xws li pMECS, pComb3X, thiab pCANTAB 5E tuaj yeem muab tau, nrog rau cov kab mob xws li TG1 E. coli, XL1 Blue, thiab ER2738. Ntxiv rau lub peev xwm tsev qiv ntawv txog li 10 ^ 9, qhov nrawm ntawm lub hom phiaj ntawm lub tsev qiv ntawv kuj siab, tsim cov xwm txheej txaus rau kev tshuaj ntsuam cov tshuaj tiv thaiv uas ua rau cov neeg siv khoom txaus siab. Daim duab ntawm Alpha Lifetech qhov kev pabcuam npaj tshuaj tiv thaiv kab mob raws li phage display technology tau pom hauv Daim Duab 1.

Daim Duab 1 Daim duab qhia txog kev npaj cov tshuaj monoclonal antibodies raws li cov thev naus laus zis phage display.

Phage Display Antibody Production Workflow

Cov kauj ruam	Cov Ntsiab Lus Kev Pabcuam	Lub Sijhawm
Kauj Ruam 1: Kev txhaj tshuaj tiv thaiv kab mob rau tsiaj	(1) Txhaj tshuaj tiv thaiv kab mob rau tsiaj 4 zaug, txhaj tshuaj ntxiv 1 koob, tag nrho 5 koob tau txhaj tshuaj lawm. (2) Cov ntshav tsis zoo ua ntej txhaj tshuaj tiv thaiv kab mob tau sau, thiab ELISA tau ua rau koob tshuaj thib plaub los ntes cov ntshav titer. (3) Yog tias cov tshuaj tiv thaiv kab mob hauv cov ntshav ntawm koob tshuaj thib plaub ua tau raws li qhov yuav tsum tau ua, yuav muab ib koob tshuaj ntxiv rau 7 hnub ua ntej sau ntshav. Yog tias nws tsis ua tau raws li qhov yuav tsum tau ua, kev txhaj tshuaj tsis tu ncua yuav txuas ntxiv mus. (4) Muaj peev xwm ua tau zoo, sau cov ntshav thiab sib cais cov monocytes	10 lub lis piam
Kauj Ruam 2: Kev Npaj cDNA	(1) PBMC Tag Nrho RNA Extraction (RNA Extraction Kit) (2) Kev npaj RT-PCR siab ntawm cDNA (cov khoom siv sau rov qab)	1 hnub
Kauj Ruam 3: Kev Tsim Lub Tsev Qiv Ntawv Antibody	(1) Siv cDNA ua tus qauv, cov noob caj noob ces tau raug nthuav dav los ntawm ob qhov kev sib hloov ntawm PCR. (2) Kev tsim kho thiab kev hloov pauv ntawm phage: kev sib txuas ntawm cov noob caj noob ces phagemid, kev hloov pauv electroporation ntawm cov kab mob TG1, kev tsim kho lub tsev qiv ntawv antibody. (3) Kev Txheeb Xyuas: Xaiv 24 lub clones yam tsis xaiv, PCR txheeb xyuas tus nqi zoo + tus nqi ntxig. (4) Kev pab npaj phage: M13 phage amplification + purification. (5) Kev cawmdim ntawm lub tsev qiv ntawv zaub Phage	3-4 lub lis piam
Kauj Ruam 4: Kev Tshuaj Xyuas Lub Tsev Qiv Ntawv Antibody (3 zaug)	(1) Kev tshuaj ntsuam 3-puag ncig (kev tshuaj ntsuam theem khov kho): kev tshuaj ntsuam siab kom tshem tawm cov tshuaj tiv thaiv tsis tshwj xeeb kom ntau li ntau tau. (2) Ib qho clone amplification bacteriophage xaiv + IPTG induced expression + ELISA nrhiav tau cov clones zoo. (3) Tag nrho cov clones zoo tau raug xaiv rau kev txheeb xyuas cov noob caj noob ces.	4-5 lub lis piam

Qhov Zoo Ntawm Phage Display Technology

Alpha Lifetech muaj kev paub nplua nuj hauv kev tsim cov tshuaj tiv thaiv kab mob. Tau ntau xyoo, Alpha Lifetech tau tsim kom muaj lub platform tsim cov tshuaj tiv thaiv kab mob.

Kev Pabcuam Txhawb Nqa

Peb tuaj yeem muab cov kev pabcuam tsim kho tsev qiv ntawv tiv thaiv kab mob sib txawv raws li tsiaj thiab cov kev pabcuam tshuaj ntsuam xyuas tsev qiv ntawv tiv thaiv kab mob ntuj raws li cov neeg siv khoom xav tau.

Lub Hom Phiaj Ntau Yam

Muaj ntau yam kev pabcuam nrhiav cov tshuaj tiv thaiv kab mob: cov protein, peptides, cov molecule me me, cov kab mob, cov protein membrane, mRNA, thiab lwm yam.

Ntau yam Vectors

Kev pabcuam tsim kho tsev qiv ntawv tus kheej, peb tuaj yeem muab ntau yam bacteriophage vectors suav nrog PMECS, pComb3X, thiab pCANTAB 5E, thiab hloov kho lawv raws li cov neeg siv khoom xav tau.

Platform laus

Lub peev xwm cia khoom tuaj yeem ncav cuag 10 ^ 8-10 ^ 9, cov nqi ntxig yog txhua yam siab dua 90%, thiab qhov sib raug zoo ntawm cov tshuaj tiv thaiv tau los ntawm kev tshuaj ntsuam feem ntau yog nyob rau ntawm nM pM theem

KEV PAB CUAM TXOG

Cov Tswv Yim Txhim Kho Ntau Yam Tshuaj Tiv Thaiv Kab Mob

Kev Pabcuam Tsim Tshuaj Monoclonal Antibody

Hybridoma Technology Platform

Ib Lub Tshuab Sorting B Cell

Phage Display Antibody Development Platform

Kev tsim cov tshuaj monoclonal antibody-Alpha Lifetech

Kev Pabcuam Tsim Tshuaj Monoclonal Antibody

Peb tuaj yeem muab cov kev pabcuam tsim cov tshuaj monoclonal antibody zoo, huv si, thiab tshwj xeeb heev, suav nrog kev tsim cov tshuaj monoclonal antibodies nas thiab cov tshuaj monoclonal antibodies luav.

Hybridoma Technology Platform

Xws li kev pab cuam txhaj tshuaj tiv thaiv kab mob, kev pab npaj cov tshuaj tiv thaiv kab mob, kev ntxuav cov tshuaj tiv thaiv kab mob, kev txheeb xyuas cov tshuaj tiv thaiv kab mob siab, kev lees paub cov tshuaj tiv thaiv kab mob, thiab lwm yam

Ib Lub Tshuab Sorting B Cell

Alpha Lifetech muaj qhov zoo hauv lub sijhawm tshuaj ntsuam thiab tau txais cov tshuaj tiv thaiv zoo. Nws tuaj yeem muab kev tsim cov tshuaj antigen, kev tsim khoom, thiab kev hloov kho, kev tiv thaiv tsiaj, kev tshuaj ntsuam xyuas cov hlwb B ib leeg, kev txheeb xyuas cov hlwb ib leeg.