Taw qhia rau Synthesized Antibody Libraries

Synthesized antibody library, tseem hu ua De novo lub tsev qiv ntawv, yog ib txoj kev siv hluavtaws uas siv cov tswv yim xws li DNA synthesis lossis phage zaub los tsim thiab ua kom tiav cov tshuaj tiv thaiv kab mob sib txawv, suav nrog cov cheeb tsam hauv cheeb tsam thiab CDRs, tsis muaj kev cia siab rau cov tsev qiv ntawv tsis muaj tshuaj tiv thaiv kab mob.

Semi-synthetic antibody tsev qiv ntawv yog tsim los ntawm kev sib txuas ib txwm tshwm sim cov tshuaj tiv thaiv cov tsev qiv ntawv nrog ntau hom hluavtaws. Nws feem ntau cuam tshuam nrog kev sib txuas ntawm DNA oligonucleotides los tsim cov txheej txheem sib txawv ntawm qhov kev txiav txim siab sib txawv (CDRs), uas tom qab ntawd ua ke nrog cov txheej txheem tiv thaiv kab mob tas li muab los ntawm cov khoom siv ntuj xws li tib neeg lossis tsiaj B hlwb. Lub synthesized CDR qhia txog ntau haiv neeg rau hauv lub tsev qiv ntawv, uas ua rau cov tshuaj tiv thaiv tsom mus rau ntau yam epitopes. Lub tsev qiv ntawv semi-synthetic antibody muab kev sib haum xeeb ntawm lub ntuj muaj ntau haiv neeg ntawm lub cev tiv thaiv kab mob thiab kev tswj ntau haiv neeg ua tiav los ntawm cov txheej txheem hluavtaws.

Qhov sib txawv ntawm cov tsev qiv ntawv tsis zoo thiab cov khoom siv tshuaj tua kab mob yog nyob ntawm qhov chaw ntawm immunoglobulin noob. Lub caij no, qhov sib txawv ntawm ib lub tsev qiv ntawv ib nrab synthesized thiab synthesized antibody nyob rau hauv uas yav tas los muaj ib feem ntawm naïve antibody segments, xws li lub teeb saw los yog hnyav saw, thiab tsuas yog ib tug ntawm lawv yog synthesized nyob rau hauv vitro; thaum lub synthesized ib yog tag nrho muab los ntawm ib tug dag synthesis los ntawm PCR nyob rau hauv vitro.

Alpha Lifetech tuaj yeem muab

Alpha Lifetech Inc.tuaj yeem muab lub tsev qiv ntawv semi-synthesized / synthesized antibody los ntawm cov tsiaj thiab tib neeg raws li peb cov kws tshaj lij antibody discovery platform. Nrog xyoo dhau los ntawm kev hloov pauv caj ces thiab kev tsim kho tsev qiv ntawv, cov tsev qiv ntawv sib xyaw ua ke tuaj yeem tsim tau uas muaj peev xwm tsim cov tshuaj tiv thaiv kab mob nrog kev sib raug zoo thiab tshwj xeeb tshaj li lub peev xwm ntawm cov tshuaj tiv thaiv ntuj.Alpha Lifetech yogCov kws tshaj lij txaus siab tshaj tawm tias peb tuaj yeem lav qhov kev ua tiav siab hauv kev tsim kho lub tsev qiv ntawv tshuaj tiv thaiv kab mob uas muaj 10^8 - 10^10 tus kheej clones.

Alpha Lifetech Inc.txaus siab muab cov kev pabcuam hauv tsev qiv ntawv tshuaj tiv thaiv kab mob kom ua tau raws li cov kev xav tau ntawm cov neeg tshawb fawb thoob ntiaj teb, suav nrog scFv, Fab, VHH antibody, thiab cov tsev qiv ntawv kho. Peb lub hom phiaj yog kom nkag siab thiab ua tau raws li qhov xav tau ntawm cov neeg siv khoom sib txawv thiab pab daws teeb meem yav tom ntej thiab tshwm sim hauv kev tshawb fawb ua haujlwm.

Synthesized Antibody Libraries Tsim Kev Pabcuam Txheej Txheem

Tsim
Cov theem tsim muaj xws li xaiv cov qauv tshuaj tiv thaiv kab mob thiab cov cheeb tsam kev txiav txim siab ntxiv (CDRs) raws li cov kab mob antibody uas twb muaj lawm lossis cov ntaub ntawv tsim qauv. Synthetic CDR cov tshuaj tiv thaiv kuj tuaj yeem tsim los qhia cov haujlwm tshwj xeeb lossis txhim kho cov khoom sib txuas.

Synthesis
Synthetic DNA encoding tus tsim cov tshuaj tiv thaiv kab mob, suav nrog ob lub hauv paus cheeb tsam thiab CDRs, yog tsim los siv tshuaj los yog enzymatic txoj kev.

Kev sib dhos
Cov khoom sib txuas DNA tau sib sau ua ke rau hauv cov tshuaj tiv thaiv kab mob qhia vectors siv cov tswv yim xws li PCR, ligation, lossis Gibson sib dhos. Cov vectors no tuaj yeem nkag mus rau hauv cov kab lus qhia xws li kab mob, cov poov xab, lossis cov kab mob mammalian rau kev tsim cov tshuaj tiv thaiv.

Kev tshuaj ntsuam thiab xaiv
Cov tsev qiv ntawv tshuaj tiv thaiv kab mob tsim tau raug tshuaj xyuas thiab xaiv rau cov tshuaj tiv thaiv kab mob uas xav tau los siv cov kev tshuaj ntsuam xyuas siab. Qhov no tej zaum yuav koom nrog cov txheej txheem xws li phage zaub, poov xab zaub, los yog ribosome zaub, nyob ntawm seb lub tsev qiv ntawv hom thiab daim ntawv thov.

Synthesized Antibody Libraries Construction Service

Alpha Lifetech Inc.muab cov neeg siv khoom nrog ib qho kev tsim qauv thiab tsim cov kev pabcuam ntawm cov tsev qiv ntawv tshuaj tiv thaiv los ntawm ligating cov hnyav thiab lub teeb saw V-gene repertoires rau hauv phage vector siv Cre-lox site-specific recombination system los tsim cov repertoire ntawm Fabs tso tawm ntawm phage suav nrog 6.5 × 10^10 clones. Lub tsev qiv ntawv yielded Abs tawm tsam ntau antigens, qee qhov nrog nanomolar affinities. Lub cim ntawm tib neeg lub tsev qiv ntawv tshuaj tiv thaiv kab mob yog ua raws li M13 phage zaub thev naus laus zis (pom hauv daim duab hauv qab).