Kev Taw Qhia rau Cov Tsev Qiv Ntawv Antibody Synthesized

Cov tsev qiv ntawv tshuaj tiv thaiv kab mob uas tsim los ntawm cov tshuaj, tseem hu ua De novo tsev qiv ntawv, yog ib txoj kev tsim uas siv cov txheej txheem xws li DNA synthesis lossis phage display los tsim thiab tsim cov cheeb tsam hloov pauv ntawm cov tshuaj tiv thaiv kab mob tag nrho, suav nrog cov cheeb tsam framework thiab CDRs, yam tsis tas yuav siv cov tsev qiv ntawv tshuaj tiv thaiv kab mob naïve.

Lub tsev qiv ntawv antibody semi-synthetic yog tsim los ntawm kev sib koom ua ke ntawm cov tsev qiv ntawv antibody uas tshwm sim hauv ntuj nrog rau ntau yam synthetic. Feem ntau nws cuam tshuam nrog kev tsim cov DNA oligonucleotides los tsim ib pawg ntawm cov cheeb tsam sib txawv sib txawv (CDRs), uas tom qab ntawd raug sib xyaw nrog cov qauv antibody tas li los ntawm cov khoom siv ntuj xws li tib neeg lossis tsiaj B hlwb. Lub CDR synthesized qhia txog ntau yam rau hauv lub tsev qiv ntawv, ua rau cov tshuaj tiv thaiv kab mob tsom mus rau ntau yam epitopes. Lub tsev qiv ntawv antibody semi-synthetic muab kev sib haum xeeb ntawm ntau yam ntuj tsim ntawm lub cev tiv thaiv kab mob thiab ntau yam tswj tau ua tiav los ntawm cov txheej txheem synthetic.

Qhov sib txawv ntawm cov tsev qiv ntawv antibody naïve thiab cov khoom siv hluavtaws nyob ntawm qhov chaw ntawm cov noob immunoglobulin. Lub caij no, qhov sib txawv ntawm cov tsev qiv ntawv antibody semi-synthesized thiab synthesized yog tias tus qub muaj ib feem ntawm cov ntu antibody naïve, xws li cov saw hlau lossis cov saw hlau hnyav, thiab tsuas yog ib qho ntawm lawv tau tsim hauv vitro; thaum tus synthesized yog tag nrho los ntawm kev tsim khoom cuav los ntawm PCR hauv vitro.

Alpha Lifetech tuaj yeem muab tau

Alpha Lifetech Inc.muaj peev xwm muab cov tsev qiv ntawv antibody semi-synthesized / synthesized los ntawm cov tsiaj thiab tib neeg raws li peb lub platform tshawb pom antibody tshaj lij. Nrog ntau xyoo ntawm kev paub hauv kev hloov pauv caj ces thiab kev tsim kho tsev qiv ntawv, cov tsev qiv ntawv synthesized tuaj yeem tsim tau uas muaj peev xwm tsim cov tshuaj tiv thaiv synthetic nrog affinity thiab tshwj xeeb tshaj li lub peev xwm ntawm cov tshuaj tiv thaiv ntuj.Alpha Lifetech'sCov kws tshaj lij zoo siab tshaj tawm tias peb tuaj yeem lav qhov ua tiav siab hauv kev tsim lub tsev qiv ntawv antibody uas muaj 10 ^ 8 - 10 ^ 10 cov clones ywj pheej.

Alpha Lifetech Inc.zoo siab los muab cov kev pabcuam tsim cov tsev qiv ntawv antibody kom ua tau raws li ntau yam kev xav tau ntawm cov kws tshawb fawb thoob ntiaj teb, suav nrog scFv, Fab, VHH antibody, thiab cov tsev qiv ntawv kho kom haum. Peb lub hom phiaj yog kom nkag siab thiab ua tau raws li qhov xav tau ntawm cov neeg siv khoom sib txawv thiab pab nrog txhua yam teeb meem uas yuav tshwm sim hauv kev tshawb fawb.

Cov Txheej Txheem Tsim Kho Cov Tsev Qiv Ntawv Antibody Synthesized

Tsim Qauv
Cov theem tsim qauv suav nrog kev xaiv cov qauv tshuaj tiv thaiv kab mob thiab cov cheeb tsam txiav txim siab sib txuas (CDRs) raws li cov kab ke tshuaj tiv thaiv kab mob uas twb muaj lawm lossis cov ntaub ntawv qauv. Cov tshuaj tiv thaiv CDR tsim kuj tseem tuaj yeem tsim los qhia cov haujlwm tshwj xeeb lossis txhim kho cov khoom khi.

Kev sib xyaw ua ke
Cov DNA tsim los ntawm kev siv cov tshuaj tiv thaiv kab mob uas tau tsim, suav nrog ob qho tib si thaj chaw thiab CDRs, yog tsim los ntawm kev siv tshuaj lom neeg lossis cov txheej txheem enzymatic.

Sib dhos
Cov DNA uas tau tsim los ua ke tau muab sib sau ua ke rau hauv cov tshuaj tiv thaiv kab mob siv cov txheej txheem xws li PCR, ligation, lossis Gibson assembly. Cov tshuaj tiv thaiv kab mob no ces tuaj yeem raug coj mus rau hauv cov kab ke qhia tawm xws li cov kab mob bacterial, yeast, lossis mammalian cells rau kev tsim cov tshuaj tiv thaiv kab mob.

Kev Tshuaj Xyuas thiab Kev Xaiv
Cov tsev qiv ntawv antibody uas tau tsim los raug tshuaj xyuas thiab xaiv rau cov tshuaj antibody uas muaj cov khoom xav tau siv cov txheej txheem tshuaj ntsuam xyuas siab. Qhov no yuav suav nrog cov txheej txheem xws li phage display, yeast display, lossis ribosome display, nyob ntawm hom ntawv tsev qiv ntawv thiab daim ntawv thov.

Kev Pabcuam Tsim Kho Cov Tsev Qiv Ntawv Antibody Synthesized

Alpha Lifetech Inc.muab rau cov neeg siv khoom nrog ib qho chaw tsim thiab kev pabcuam synthesized ntawm cov tsev qiv ntawv antibody los ntawm kev sib txuas cov hnyav thiab lub teeb saw V-gene repertoires rau hauv phage vector siv Cre-lox qhov chaw-tshwj xeeb recombination system los tsim cov repertoire ntawm Fabs tso tawm ntawm phage uas muaj 6.5 × 10 ^ 10 clones. Lub tsev qiv ntawv tau tsim Abs tawm tsam ntau antigens, qee qhov nrog nanomolar affinities. Lub cim ntawm tib neeg lub tsev qiv ntawv antibody yog raws li M13 phage zaub technology (qhia hauv daim duab hauv qab no).