Gịnị bụ Injinịa Antibody?

Site n'enyemaka nke injinịa antibody, o kwela omume ịgbanwe nha molekul, pharmacokinetics, immunogenicity, njikọ njikọ, nkọwapụta na ọrụ arụmọrụ nke antibody. Mgbe emechara njikọta antibody, njikọ kpọmkwem nke antibody na-eme ka ha baa uru nke ukwuu na nchọpụta na ọgwụgwọ ahụike. Site na injinịa antibody, ha nwere ike igbo mkpa nke ọgwụ na mmepe mmalite nke nchọpụta.

Ebumnuche nke injinịa antibody bụ imepụta ma mepụta ọrụ ndị a kapịrị ọnụ, ndị kwụsiri ike nke ọgwụ mgbochi sitere n'okike na-enweghị ike imezu, na-atọ ntọala maka mmepụta nke ọgwụ mgbochi ọgwụgwọ.

Alpha Lifetech, site na ahụmịhe ọrụ ya dị ukwuu na injinia antibody, nwere ike inye ọrụ antibody monoclonal na polyclonal ahaziri maka ọtụtụ ụdị, yana ọrụ owuwu na nyocha ọbá akwụkwọ antibody ngosi phage. Alpha Lifetech nwere ike inye ndị ahịa ọgwụ mgbochi biosimilar dị mma na ngwaahịa protein recombinant, yana ọrụ ndị kwekọrọ, iji mepụta ọgwụ mgbochi dị irè, nke a kapịrị ọnụ, na nke kwụsiri ike. Site na iji antibody zuru oke, ikpo okwu protein na sistemụ ngosipụta phage, anyị na-enye ọrụ na-ekpuchi mmepụta antibody elu na ala, gụnyere ọrụ teknụzụ dịka ime ka antibody dị mma, nhicha antibody, usoro antibody, na nkwenye antibody.

Usoro injinịa antibody nke mbụ metụtara teknụzụ abụọ:

--Teknụzụ DNA recombinant

--Teknụzụ Hybridoma

Mmepe ngwa ngwa nke injinịa antibody metụtara teknụzụ atọ dị mkpa:

--Teknụzụ mmepụta mkpụrụ ndụ ihe nketa na mmeghachi omume polymerase

--Nkọwapụta protein: A na-emepụta protein ndị na-agbanwe agbanwe site na sistemụ ngosipụta dịka yist, nje virus ndị yiri mkpanaka, na osisi

-- Nhazi ihe owuwu enyere aka na kọmputa

E mere ka ọgbọ mbụ nke ọgwụ mgbochi ọrịa dịkwuo mma maka mmepụta nke ọgwụ mgbochi chimeric, ebe mpaghara mgbanwe nke ọgwụ mgbochi monoclonal òké jikọtara ya na mpaghara na-adịgide adịgide nke mkpụrụ ndụ ihe nketa IgG mmadụ. E tinyere mpaghara njikọ antigen (CDR) nke ọgwụ mgbochi monoclonal òké nke ọgbọ nke abụọ n'ime IgG mmadụ. E wezụga mpaghara CDR, ọgwụ mgbochi ọrịa ndị ọzọ niile bụ ihe fọrọ nke nta ka ọ bụrụ ọgwụ mgbochi ọrịa mmadụ, a gbalịkwara izere ịkpalite mmeghachi omume antibody anti òké mmadụ (HAMA) mgbe ejiri ọgwụ mgbochi clone òké maka ọgwụgwọ mmadụ.

 

Iji wuo ọbá akwụkwọ ngosi phage, nzọụkwụ mbụ bụ inweta mkpụrụ ndụ ihe nketa na-egosi ihe mgbochi, nke enwere ike ikewapụ na mkpụrụ ndụ B nke anụmanụ a gbara ọgwụ mgbochi (iwu ụlọ akwụkwọ mgbochi), ewepụtara ozugbo site na anụmanụ ndị na-abụghị ndị a gbara ọgwụ mgbochi (iwu ụlọ akwụkwọ eke), ma ọ bụ ọbụna jikọta ya na in vitro na iberibe mkpụrụ ndụ ihe nketa antibody (iwu ụlọ akwụkwọ sịntetik). Mgbe ahụ, a na-eme ka mkpụrụ ndụ ihe nketa dịkwuo elu site na PCR, tinye ya na plasmids, ma gosipụta ya na sistemụ nnabata kwesịrị ekwesị (okwu yist (na-abụkarị Pichia pastoris), okwu prokaryotic (na-abụkarị E. coli), okwu mkpụrụ ndụ mammalian, okwu mkpụrụ osisi, na okwu mkpụrụ ndụ ahụhụ nke nje virus dị ka mkpanaka butere). Nke kachasị bụ sistemụ ngosipụta E. coli, nke na-ejikọta usoro ihe mgbochi nzuzo kpọmkwem na phage ma na-akpọpụta otu n'ime protein shei phage (pIII ma ọ bụ pVIII). Njikọta mkpụrụ ndụ ihe nketa nke, Na-egosipụtakwa n'elu bacteriophages. Isi ihe dị na teknụzụ a bụ iwu ọbá akwụkwọ ngosi phage, nke nwere uru karịa ọbá akwụkwọ eke n'ihi na ọ nwere ike inwe njikọ pụrụ iche. Mgbe nke ahụ gasịrị, a na-enyocha ọgwụ mgbochi nwere kpọmkwem antigen site na usoro nhọrọ bayọlọji, a na-edozi antigens ezubere iche, a na-asachapụ phages ndị na-enweghị njikọ ugboro ugboro, a na-asachapụ phages ndị e jikọtara ọnụ maka mmụba ọzọ. Mgbe agba atọ ma ọ bụ karịa nke ugboro ugboro gasịrị, a na-ekewapụ phages ndị nwere nkọwapụta dị elu na antigen ndị nwere njikọ dị elu.

Foto nke 3: Ịrụ na Nnyocha Ọbá Akwụkwọ Mgbochi Ahụ

Enwere ike iji teknụzụ DNA recombinant mepụta iberibe antibody. Na mbụ, a na-eme ka ọgwụ mgbochi Fab dị na mmiri site na protease gastric iji mepụta iberibe abụọ (Fab '), nke papain na-agbari iji mepụta iberibe Fab nke ọ bụla. Mpekere Fv nwere VH na VL, nke na-enweghị nkwụsi ike zuru oke n'ihi enweghị njikọ disulfide. Ya mere, a na-ejikọ VH na VL ọnụ site na obere peptide nke amino acid 15-20 iji mepụta otu antibody nke nwere nnukwu mgbanwe agbụ (scFv) nke nwere oke molekul nke ihe dị ka 25KDa.

Ọmụmụ ihe gbasara nhazi ọgwụ mgbochi na Camelidae (Camel, LIama, na Alpaca) egosila na ọgwụ mgbochi nwere naanị agbụ ígwè dị arọ na enweghị agbụ ígwè dị ọkụ, ya mere a na-akpọ ha ọgwụ mgbochi agbụ ígwè dị arọ (hcAb). A na-akpọ ngalaba mgbanwe nke ọgwụ mgbochi agbụ ígwè dị arọ otu mgbochi ma ọ bụ nanobodies ma ọ bụ VHH, nke nwere nha 12-15 kDa. Dịka monomers, ha enweghị njikọ disulfide ma kwụsie ike nke ukwuu, ha nwekwara njikọ dị elu maka antigens.

Foto nke 5: Ọgwụ Mgbochi Agbụ Agbụ na VHH/Nanobody

Nnwere onwe mkpụrụ ndụ na-eji ngosipụta nke DNA eke ma ọ bụ nke ejiri aka mee iji nweta njikọ protein in vitro, nke na-ejikarị usoro ngosipụta E. coli. Ọ na-emepụta protein ngwa ngwa ma na-ezere ibu metabolic na cytotoxic na mkpụrụ ndụ mgbe ọ na-emepụta nnukwu protein recombinant na vivo. Ọ nwekwara ike imepụta protein ndị siri ike imepụta, dịka ndị siri ike ịgbanwe mgbe a tụgharịrị ma ọ bụ mepụta protein membrane.