Ọrụ Nyocha Njirimara Aptame

Alpha Lifetech anọwo na-enye ọrụ metụtara aptamer ruo ọtụtụ afọ, ma ugbu a ọ nwere ike inye atụmatụ mmepe aptamer zuru oke dịka njikọta aptamer, nyocha SELEX, usoro mmepụta dị elu, na nhazi na njirimara aptamer. Dabere na uru nke usoro SELEX maka nyocha aptamers, Alpha Lifetech amụbaala ụzọ ndị ọzọ maka inyocha aptamers.

Okwu Mmalite na Nyocha Njirimara Aptamer

Nkwenye mmekọ nwoke na nwanyị

E jiri usoro ndị dị mkpa nke nnwale njikọta aptamer gosi na njikọta aptamer kachasị mma. Ihe atụ gụnyere Isothermal Titration Calorimetry (ITC), Flow Cytometry (FCM), na Surface Plasmon Resonance (SPR), Microfluidics, wdg. A na-egosipụta njikọta Aptamer site na constant dissociation (KD), ọnụọgụ anụ ahụ eji atụ ọkwa nke nkewa molekul na mmeghachi omume a na-agbanwe agbanwe. Ka uru KD pere mpe, otú ahụ ka mgbagwoju anya ahụ si kwụsie ike, ya bụ, ike nkewa ahụ sikwuo ike; N'aka nke ọzọ, ka uru KD buru ibu, otú ahụ ka mgbagwoju anya ahụ na-adịghị agbanwe agbanwe na ike nkewa ahụ sikwuo ike. Nzọụkwụ a bụ isi ihe na-eme ka a mata arụmọrụ nke aptamer, na-ahụ na ntozu oke nke aptamer ma nwee ike ijikọ na molekul ebumnuche nwere oke njikọ na nhọrọ na ngwa bara uru.

Fig.1 Usoro ntozu oke nke aptamer. Isi mmalite ntụaka:Kinghorn AB, Fraser LA, 2017.

Nkwenye ọrụ

Na mgbakwunye na nkwenye njikọta, ọ dị mkpa ka a chọpụta ọrụ nke aptamer. Nke a gụnyere inyocha nkwụsi ike nke aptamer, nkọwapụta ya, na mmekọrịta ya na molekul ndị ọzọ n'otu gburugburu. Nsonaazụ nke nkwenye ọrụ ga-emetụta kpọmkwem uru nke aptamers na nyocha sayensị na ngwa ụlọ ọrụ mmepụta ihe.

Nkwenye a kapịrị ọnụ

Nnwale asọmpi: A na-enyocha ikike nke aptamer ijikọta na ihe mgbaru ọsọ kpọmkwem ma ọ bụrụ na ihe mgbaru ọsọ ndị ọzọ yiri ya dị. Ọ bụrụ na aptamer nwere ike ijikọta kpọmkwem na ihe mgbaru ọsọ na-enweghị nnyonye anya site na ihe ndị ọzọ, ọ na-egosi na ọ nwere nkọwapụta dị elu.

Nnwale mmeghachi omume nke otu ihe: A na-ejikọta ihe aptamer na usoro ihe mgbaru ọsọ ndị metụtara ya ma ọ bụ ndị na-enweghị njikọ iji hụ ma ọ na-ejikọta naanị na ihe mgbaru ọsọ kpọmkwem, iji chọpụta kpọmkwem ya.

Nkwenye kwụsiri ike

Nnwale mbibi nuklia: A hụrụ aptamers ahụ n'ọtụtụ nuclease dị iche iche ma hụ mmebi ha. Site n'ịtụnyere oke mbibi n'oge dị iche iche, enwere ike inyocha ikike mmebi nuklia nke aptamer.

Nnwale ọnọdụ okpomọkụ na oge: E tinyere aptamer n'okpuru ọnọdụ okpomọkụ na oge dị iche iche iji hụ na ọ kwụsiri ike n'usoro na n'ọrụ ya. Nke a na-enyere aka ikpebi ọnọdụ nchekwa na ojiji kacha mma maka aptamers.

Nchọpụta ọrụ ihe ndị dị ndụ

A na-ahọrọkarị usoro kwesịrị ekwesị dịka ebumnuche ntinye nke aptamer nucleic acid si dị.

(1) Nnwale ọkwa molekul: Iji usoro bayoloji molekul, dị ka gel electrophoresis, Western blot, wdg, chọpụta ihe mgbagwoju anya ndị e mepụtara mgbe aptamer gwakọtara ya na molekul ebumnuche, ma ọ bụ chọpụta mgbanwe na ọkwa ngosipụta nke molekul ebumnuche nke aptamer kpatara.

(2)Nnwale ọkwa mkpụrụ ndụ: Site na iji teknụzụ ọdịbendị mkpụrụ ndụ, a na-etinye mkpụrụ ndụ ndị aptamers na mkpụrụ ndụ ndị a na-elekwasị anya iji hụ mgbanwe ndị dị ndụ dịka ọdịdị mkpụrụ ndụ, mmụba na apoptosis, ma nyochaa ọrụ nke ndị aptamers.

(3)Nnwale ihe nlereanya anụmanụ: N'ihe nlereanya anụmanụ kwesịrị ekwesị, a na-enye ihe nrịbama nucleic acid site na ịgba ọgwụ ma ọ bụ inye ọgwụ, a na-ahụkwa ihe ngosi physiological na mgbanwe ọrịa nke anụmanụ iji nyochaa ọrụ na nchekwa nke ihe nrịbama na vivo.