Na 1960, e wepụtara echiche nke ọgwụ mgbochi bispecific. Ọgwụ mgbochi bispecific, nke a makwaara dị ka ọgwụ mgbochi monoclonal bispecific, bụ ọgwụ mgbochi e ji aka mepụta site na iji teknụzụ injinịa mkpụrụ ndụ ihe nketa. Dịka ọgwụ mgbochi artificial engineering, ọgwụ mgbochi bispecific na-abụkarị nke obere klas IgG ma nwee akụkụ njikọ antigen na-elekwasị anya na subunit CD3. Ọgwụ mgbochi bispecific nwere ebe njikọ antigen abụọ akọwapụtara nke nwere ike ijikọ ma mata antigen abụọ dị iche iche n'otu oge, ma ọ bụ epitopes abụọ dị iche iche nke antigen. Ma e jiri ya tụnyere ọgwụ mgbochi monoclonal, ọgwụ mgbochi bispecific nwere ebe njikọ antigen ọzọ akọwapụtara, si otú a nwee nkọwapụta siri ike na ikike ebumnuche, nke nwere ike ilekwasị anya na mkpụrụ ndụ tumor nke ọma ma belata nsí ebumnuche. Ọgwụ mgbochi bispecific nwere ike ịrụ ọrụ dị iche iche n'otu oge, dị ka ịchọta mkpụrụ ndụ na-alụso ọrịa ọgụ, igbochi ụzọ mgbaàmà, na igbu mkpụrụ ndụ tumor ozugbo. A na-akwadebe ọgwụ mgbochi bispecific mbụ site na njikọta kemịkalụ ma ọ bụ njikọ mkpụrụ ndụ, mana usoro a nwere ike ịga n'ihu nwayọ n'ihi njikọta na-enweghị usoro na ihe isi ike n'ịwapụ njikọta ebumnuche. Site na mmepe na-aga n'ihu nke teknụzụ injinịa mkpụrụ ndụ ihe nketa, e mepụtala ọtụtụ ikpo okwu teknụzụ ọhụrụ, dị ka knots in holes (KIH), CrossMab, DVD Ig, wdg. Ikpo okwu ndị a na-edozi nsogbu dị ka agbụ ígwè dị arọ na enweghị njikọ nke agbụ ígwè dị mfe, ma na-eme ka otu na mmepụta nke ọgwụ mgbochi bispecific dịkwuo mma.

Ụzọ ndị bụ isi maka mmepụta ọgwụ mgbochi bispecific gụnyere njikọ kemịkalụ, hybridoma isi anọ, na nkwadebe antibody engineering genetic engineering. N'ime ha, usoro njikọ kemịkalụ na-ejikọ iberibe antibody IgG abụọ ma ọ bụ F (ab ') abụọ na-enweghị nsogbu n'ime ọgwụ mgbochi bispecific site na iji ihe njikọta kemịkalụ dị ka phthalimide na dithioacylbenzoic acid. Usoro a dị mfe ma dị mfe ịrụ ọrụ, mana ọ nwere ike imebi ebe njikọ antigen, belata ọrụ antibody, ihe njikọ ahụ n'onwe ya nwekwara oke nke kansa. Usoro hybridoma isi anọ dabere na njikọta nke mkpụrụ ndụ somatic site na ahịrị mkpụrụ ndụ hybridoma abụọ dị iche iche iji gosipụta òké IgG kwekọrọ. Site na teknụzụ injinia mkpụrụ ndụ ihe nketa, enwere ike ịgbanwe antibody mkpụrụ ndụ ihe nketa ka ọ mepụta antibody bispecific. E wuru antibody monoclonal abụọ dị iche iche, e kewarakwa iberibe Fab ma ọ bụ nnukwu yinye na mpaghara mgbanwe nke yinye ọkụ nke ọgwụ mgbochi abụọ ahụ iche. Site na mmeghachi omume njikọ ma ọ bụ teknụzụ njikọta yinye, ejikọtara iberibe abụọ ahụ iji mepụta antibody bispecific. Ọ bụ ezie na mgbagwoju anya nke teknụzụ injinia mkpụrụ ndụ ihe nketa dị elu, ọ bụ ugbu a usoro a na-ejikarị eme ihe maka mmepụta antibody bispecific iji gbanwee nhazi na ọrụ nke antibody. Mgbe a na-eme nhazi ọgwụ mgbochi bispecific, a pụrụ iji ụkpụrụ nke mmeghachi omume antibody cross reactivity mee ihe, mana mmeghachi omume antibody cross reactivity nwere ike ibute mmeghachi omume na-abụghị nke akọwapụtara, yabụ a ga-atụle ya nke ọma n'ọrụ bara uru nke imepụta ọgwụ mgbochi bispecific, dịka ọgwụgwọ antibody bispecific.

Nhicha ihe mgbochi nje nke bispecific bụ usoro nke ikewapụ na ime ka ihe mgbochi nje dị ọcha nke ukwuu dị ọcha. A na-eji ụzọ abụọ, centrifugation na nzacha miri emi, ewepụ ụfọdụ ihe na-agbaze agbaze. A na-ejide ihe mgbochi nje nke bispecific na mbụ site na iji affinity chromatography. Maka ihe mgbochi nje nke bispecific dị ka IgG, a na-eji protein A affinity chromatography, ebe maka ihe mgbochi nje nke na-abụghị IgG dị ka bispecific, enwere ike iji chromatography affinity based light yinye. Mgbe nke ahụ gasịrị, a na-etinye ihe mgbochi nje ahụ n'okpuru ọnọdụ pH dị ala maka oge ụfọdụ, na-eme ka nhazi protein dị n'elu envelopu nje ahụ ghara ịdị irè, si otú a na-efunahụ ikike ibute mkpụrụ ndụ. A na-eme nzacha miri emi dị n'etiti iji wepụ ihe ndị na-adịghị ọcha dị ka protein sel host (HCP). A na-eme ka ịdị ọcha nke ihe mgbochi nje dịkwuo mma site na usoro dị ka ion exchange chromatography, a na-ewepụkwa nje ọ bụla fọdụrụ site na usoro nanofiltration ma ọ bụ ultrafiltration. N'ikpeazụ, a na-etinye ihe nlele ahụ ma jiri ihe nchekwa nhazi kwesịrị ekwesị dochie ya.