Enwere ike iji teknụzụ Hybridoma kwadebe ọgwụ mgbochi monoclonal. Site na ijikọ mkpụrụ ndụ splin site na òké ndị a gbanyere ọgwụ mgbochi yana mkpụrụ ndụ myeloma site na mmadụ ma ọ bụ òké, a na-emepụta mkpụrụ ndụ hybridoma ma na-ewepụta ọgwụ mgbochi ụfọdụ. Enwere ike iji ọgwụ mgbochi monoclonal nke òké mepụtara mee ihe na nyocha sayensị dịka nnwale immunosorbent enzyme-linked, radioimmunoassay, immunohistochemistry, flow cytometry, purification protein, wdg. Ihe nlele antibody monoclonal gụnyere ascites, hybridoma supernatant, wdg. Ihe nlele e nwetara site na ịgbanye mkpụrụ ndụ hybridoma n'ime oghere afọ nke anụmanụ na iji gburugburu ebe obibi nke oghere afọ anụmanụ mepụta ọgwụ mgbochi a na-akpọ ascites sample. Mkpụrụ ndụ Hybridoma a gbanyere n'ime oghere afọ nke anụmanụ nwere ike ịba ụba ma wepụta ọgwụ mgbochi monoclonal, nke nwere ike ịgbakọta na ascites. N'ihi ohere nke ihe na-adịghị ọcha (protein anụmanụ, homonụ, wdg) na ọgwụ mgbochi ndị a, a chọrọ nhicha. Dabere na ọrụ nzuzo nke lymphocytes B, a na-etinyekarị ọgwụ mgbochi monoclonal n'ime usoro ọdịbendị mgbe a na-azụ mkpụrụ ndụ hybridoma. A na-akpọ mmiri nwere ọgwụ mgbochi monoclonal na usoro omenala hybridoma supernatant. Ịdị ọcha nke ọgwụ mgbochi monoclonal dị n'elu ihe mkpuchi ahụ dị elu, mana ọ dị mkpa ka e mee ka ọ dị ọcha ọzọ maka nkwadebe buru ibu ebe ọ bụ na a na-enweta ha site na ọdịbendị in vitro.

Nhicha ihe mgbochi ahụ nwere ike ikewapụ ihe mgbochi dị ọcha site na mgbagwoju anya. Ugbu a, nhicha ihe mgbochi ahụ na nkewa na nhicha ihe mgbochi bụ ụzọ a na-ejikarị eme ihe maka nhicha ihe mgbochi ahụ, ọkachasị site na nhicha ihe mgbochi protein A/G, chromatography mgbanwe ion, nzacha gel, mmiri ozuzo na chromatography mmekọrịta hydrophobic. N'ime ha, ọrụ nhicha ihe mgbochi protein A/G affinity na-ewu ewu nke ukwuu na ngwa nhicha ihe mgbochi dị iche iche. Usoro nke Alpha Lifetech na-akwadebe ihe mgbochi monoclonal dabere na teknụzụ hybridoma ka egosiri na Foto 1.

Fig.1. Usoro nhazi maka ịkwadebe ọgwụ mgbochi monoclonal dabere na teknụzụ hybridoma.