Ikpo Okwu Mmepe Ọgwụ Mgbochi nke Phage Display
Dabere na usoro ikpo okwu zuru oke nke ikpo okwu antibody, Alpha Lifetech nwere ike inye ọrụ teknụzụ site na nkwadebe antibody, nhicha antibody, usoro antibody, wdg.
KPỌTỤRỤ ANYỊ01
Ikpo Okwu Mmepe Ọgwụ Mgbochi nke Phage Display
Alpha Lifetech, site na ahụmịhe ọrụ ya dị ukwuu n'ịchọpụta ihe gbasara ọgwụ mgbochi, nwere ike inye ọrụ ọgwụ mgbochi monoclonal na polyclonal ahaziri maka ọtụtụ ụdị, yana ọrụ owuwu na nyocha ọbá akwụkwọ ọgwụ mgbochi. Alpha Lifetech nwere ike inye ndị ahịa ngwaahịa ọgwụ mgbochi na ọgwụ mgbochi recombinant dị mma, yana ọrụ ndị kwekọrọ, iji kwadebe ọgwụ mgbochi dị irè, nke a kapịrị ọnụ, na nke kwụsiri ike. Site na iji ikpo okwu ọgwụ mgbochi zuru oke na injinịa ọgwụ mgbochi, anyị na-enye ọrụ na-ekpuchi mmepụta ọgwụ mgbochi dị elu na nke dị ala, gụnyere ọrụ teknụzụ dịka nkwadebe ọgwụ mgbochi, nhicha, usoro ọgwụ mgbochi, na nkwenye.
Alpha Lifetech nwere ikpo okwu nchọpụta ihe gbasara ọgwụ mgbochi afọ nke na-enye ndị ahịa ọtụtụ ọrụ dị iche iche gụnyere mgbochi anụmanụ, owuwu na nyocha ọbá akwụkwọ ihe ngosi phage antibody, usoro ihe mgbochi afọ, ngosipụta ihe gbasara ọgwụ mgbochi afọ, nhicha ihe mgbochi afọ, nkwenye ihe mgbochi afọ, na akara antibody dabere na teknụzụ hybridoma, teknụzụ otu B cell, teknụzụ ngosipụta phage, na ndị ọzọ. Alpha Lifetech nwere usoro mmepụta ọkọlọtọ, na mgbakwunye na ọrụ nkwadebe ihe mgbochi afọ, ọrụ nkwado dịka ime ka ihe mgbochi afọ, ntozu oke nke antibody, imepụta na mmepe ọgwụ ADC, yana imepụta usoro CAR-T na-esote. N'otu oge ahụ, Alpha Lifetech arụọla ọbá akwụkwọ ihe mgbochi afọ dabere na nyiwe teknụzụ ngosipụta phage M13, T4, na T7, nwere ike ịchekwa ruo 10^8-10^9. Ọnụego dị mma, ọnụego ntinye, na ụdị dị iche iche nke ọbá akwụkwọ ahụ nwere ike iru ihe karịrị 90%.
Teknụzụ ngosi nke peji
E ji teknụzụ hybridoma monoclonal antibody kwadebe mmepụta ọgwụ mgbochi monoclonal mbụ iji mepụta ọgwụ mgbochi monoclonal hybridoma òké. Karịsịa, site na ijikọ mkpụrụ ndụ spleen site na òké ndị a gbanyere ọgwụ mgbochi yana mkpụrụ ndụ myeloma site na mmadụ ma ọ bụ òké, a na-emepụta mkpụrụ ndụ hybridoma, nke na-ewepụta ọgwụ mgbochi monoclonal hybridoma kpọmkwem. Site na mgbanwe nke ọgwụ mgbochi monoclonal òké na mgbanwe injinịa mkpụrụ ndụ ihe nketa nke ọgwụ mgbochi iji nye ha mpaghara immunoglobulin mmadụ na-adịgide adịgide, enwere ike ibelata ike immunogenicity ha. A na-eji injinịa antibody monoclonal eme ihe nke ukwuu na ngwa biomedical na ọgwụgwọ.
Alpha Lifetech nwere ike inye ọrụ injinịa ọgwụ mgbochi otu nkwụsị site na nhazi usoro nyocha tupu oge eruo ruo na nchọpụta ọgwụ mgbochi nje (ADCs) na nkwenye anụmanụ. Njikọ ọgwụ mgbochi nje (ADCs) nwere ike ibuga ọgwụ chemotherapy na mkpụrụ ndụ kansa. Mgbe ejirikọta na ebumnuche akọwapụtara na mkpụrụ ndụ kansa, ADC na-ewepụta ọgwụ cytotoxic n'ime mkpụrụ ndụ kansa. Alpha Lifetech nwekwara ike inye ndị ahịa ọrụ zuru oke nke antibody affinity tojuru. Site na njikarịcha mgbanwe dị elu na teknụzụ nlele phage dị elu, a na-ebu ụzọ nyochaa antibody nwere affinity ụfọdụ, wee webata amino acid affinity iji mepụta ụdị dị iche iche. Mgbe nke ahụ gasịrị, a tụlere ma nyochaa antibody affinity dị elu site na iji usoro nyocha. Mgbe ọtụtụ agba nke njikarịcha na nyocha nhazi gasịrị, enwetara antibody nwere affinity dị elu na nkọwapụta siri ike.
Alpha Lifetech nwere ike ịmepụta ụdị ọbá akwụkwọ ngosi phage antibody dị iche iche, gụnyere ọbá akwụkwọ mgbochi, ọbá akwụkwọ obodo, ọbá akwụkwọ semi synthetic, na ọbá akwụkwọ sịntetik. Dabere na ikike dị elu nke ọbá akwụkwọ antibody, enwere ike nweta ọgwụ mgbochi pụrụ iche. Enwere ike inye ọtụtụ vector phagemid dị ka pMECS, pComb3X, na pCANTAB 5E, yana ụdị dị ka TG1 E. coli, XL1 Blue, na ER2738. Na mgbakwunye na ikike ọbá akwụkwọ nke ruru 10^9, ọnụego ntinye nke iberibe ebumnuche nke ọbá akwụkwọ ahụ dịkwa elu, na-emepụta ọnọdụ zuru oke maka nyocha ọgwụ mgbochi nke na-emeju ndị ahịa afọ. E gosipụtara eserese usoro nke ọrụ nkwadebe antibody Alpha Lifetech dabere na teknụzụ ngosipụta phage na Foto 1.
Fig.1. Usoro nhazi maka ịkwadebe ọgwụ mgbochi monoclonal dabere na teknụzụ ngosipụta phage.
Usoro Ọrụ Mmepụta Ọgwụ Mgbochi nke Phage
| Nzọụkwụ | Ọdịnaya Ọrụ | Usoro iheomume |
|---|---|---|
| Nzọụkwụ nke 1: Ọgwụ mgbochi ọrịa anụmanụ | (1) Ọgwụ mgbochi anụmanụ ugboro anọ, ọgwụ mgbochi booster otu dose, ngụkọta nke ọgwụ mgbochi ise. (2) A na-anakọta serum na-adịghị mma tupu a gbaa ọgwụ mgbochi, a na-emekwa ELISA na dose nke anọ iji chọpụta titer serum. (3) Ọ bụrụ na ọgwụ mgbochi ọbara nke ọgwụ nke anọ ruru ihe achọrọ, a ga-enye otu ọgwụ mgbochi ọzọ ụbọchị asaa tupu a chịkọta ọbara. Ọ bụrụ na ọ rughị ihe achọrọ, a ga-aga n'ihu na-agba ọgwụ mgbochi mgbe niile. (4) Ike ruru eru, nchịkọta ọbara na nkewa nke monocytes | Izu iri |
| Nzọụkwụ nke 2: Nkwadebe cDNA | (1) Mwepụta RNA PBMC zuru oke (Ngwa Mwepụta RNA) (2) Nkwadebe RT-PCR dị elu nke cDNA (ngwa ntụgharị azụ) | Otu ụbọchị |
| Nzọụkwụ nke 3: Ịrụ Ọbá Akwụkwọ Ọgwụ Mgbochi | (1) Site na iji cDNA dị ka ihe nlereanya, agba abụọ nke PCR mụbara mkpụrụ ndụ ihe nketa. (2) Nhazi na mgbanwe nke usoro: njikọta mkpụrụ ndụ ihe nketa nke usoro phagemid, mgbanwe electroporation nke nje bacteria TG1, owuwu nke ọbá akwụkwọ antibody. (3) Nchọpụta: Họrọ klọn iri abụọ na anọ n'enweghị usoro, ọnụego njirimara PCR dị mma + ọnụego ntinye. (4) Nkwadebe phage enyere aka: M13 phage amplification+purification. (5) Nnapụta ọbá akwụkwọ ngosi nke ngalaba | Izu 3-4 |
| Nzọụkwụ nke 4: Nnyocha Ọbá Akwụkwọ Mgbochi Ahụ (agba atọ) | (1) Nchọpụta agba atọ nke ndabara (nyocha usoro siri ike): nyocha nrụgide iji wepụ ọgwụ mgbochi ndị na-abụghị nke akọwapụtara ruo n'ókè kachasị ike. (2) Nchikota nje bacteria nke a họọrọ + IPTG kpaliri + nchọpụta ELISA nke ihe ndị dị mma. (3) A họpụtara klọn niile dị mma maka usoro mkpụrụ ndụ ihe nketa. | Izu 4-5 |
Ọrụ Nkwado
Anyị nwere ike inye ọrụ owuwu ọbá akwụkwọ mgbochi ọrịa dị iche iche dabere na anụmanụ yana ọrụ nyocha ọbá akwụkwọ mgbochi ọrịa eke dịka mkpa ndị ahịa si dị
Ọtụtụ Ebumnuche
Enwere ike ịchọta ọtụtụ ihe mgbochi nje dị iche iche: protein, peptides, obere molekul, nje virus, protein membrane, mRNA, wdg.
Ọtụtụ Vektọ
Ọrụ owuwu ọbá akwụkwọ nkeonwe, anyị nwere ike inye ọtụtụ vektọ bacteriophage gụnyere PMECS, pComb3X, na pCANTAB 5E, ma gbanwee ha dịka mkpa ndị ahịa si dị.
Ikpo Okwu Ndị Tozuru Okè
Ike nchekwa nwere ike iru 10 ^ 8-10 ^ 9, ọnụego ntinye niile karịrị 90%, ike nke ọgwụ mgbochi nje enwetara site na nyocha na-abụkarị n'ọkwa nM pM.
Ọrụ Mmepe Mgbochi Ahụ nke Monoclonal
Anyị nwere ike inye ọrụ mmepe monoclonal antibody dị elu, dị ọcha, na nke a kapịrị ọnụ, gụnyere mmepụta nke monoclonal antibodies òké na rabbit monoclonal antibodies
Ikpo Okwu Teknụzụ Hybridoma
Gụnyere mmemme ịgba ọgwụ mgbochi, ọrụ nkwadebe ọgwụ mgbochi, nhicha ọgwụ mgbochi, usoro mmepụta dị elu nke ọgwụ mgbochi, nkwenye ọgwụ mgbochi, wdg.
Ikpo Okwu Nhazi Cell Otu B
Alpha Lifetech nwere uru dị na oge nyocha na inweta ọgwụ mgbochi dị elu. Ọ nwere ike inye nhazi antigen, mmepụta, na mgbanwe, mgbochi nje anụmanụ, nyocha mmụba nke otu mkpụrụ ndụ B, na usoro otu mkpụrụ ndụ.
Ikpo Okwu Mmepe Ọgwụ Mgbochi nke Phage Display
Alpha Lifetech nwere ike inye ọrụ teknụzụ maka mmepe nke ihe ngosi phage antibody site na nkwadebe antibody, nhicha antibody, usoro antibody, wdg.
Ọ bụrụ na ị nwere ajụjụ ọ bụla, biko nweere onwe gị ịkpọtụrụ anyị n'oge ọ bụla.
Leave Your Message
2018-07-16 
0102