Ratonga Tātari Āhuatanga Aptamer

Kua roa a Alpha Lifetech e whakarato ana i ngā ratonga e pā ana ki ngā aptamer, ā, i tēnei wā ka taea e ia te whakarato i ngā kaupapa whakawhanake aptamer whānui pērā i te hanga aptamer, te tirotiro SELEX, te raupapatanga tere-rahi, me te arotau me te tautuhi i ngā aptamer. I runga i ngā painga o te tikanga SELEX mō te tirotiro i ngā aptamer, kua whakawhānui ake a Alpha Lifetech i ngā tikanga maha atu mō te tirotiro i ngā aptamer.

He Kupu Whakataki ki te Tātari Āhuatanga Aptamer

Manatoko hononga

I manatokohia te pai o te hononga aptamer kua arotauhia mā roto i ngā tikanga e tika ana o te whakamātautau here aptamer. Hei tauira, ko te Isothermal Titration Calorimetry (ITC), te Flow Cytometry (FCM), me te Surface Plasmon Resonance (SPR), te Microfluidics, me ētahi atu. Ko te pai o te hononga aptamer ka whakaatuhia e te pūmau wehewehe (KD), he rahinga ā-tinana e whakamahia ana hei ine i te nui o te wehewehenga ngota i roto i te tauhohenga whakahurihuri. Ko te iti o te uara KD, ko te pumau ake o te matatini, arā, ko te kaha ake o te hononga; I tetahi atu taha, ko te nui ake o te uara KD, ko te kore pumau ake o te matatini, ā, ko te ngoikore ake o te hononga. Ko tēnei taahiraa te mea nui ki te tautuhi i te mahi a te aptamer, kia whakarite ai i te pakeketanga o te hononga aptamer, ā, ka taea te here ki te ngota ūnga me te kaha me te whiriwhiri nui i roto i ngā tono mahi.

Pikitia 1 Te tukanga o te pakeketanga o te hononga aptamer. Pūtake tohutoro:Kinghorn AB, Fraser LA, 2017.

Manatoko mahi

Haunga te manatoko i te hononga, me manatoko anō hoki te mahi a te aptamer. Kei roto i tēnei ko te manatoko i te pumau, te motuhaketanga, me ngā taunekeneke o te aptamer ki ētahi atu ngota i roto i tētahi taiao motuhake. Ka pā tika ngā hua o te manatoko mahi ki te uara o ngā aptamer i roto i ngā rangahau pūtaiao me ngā tono ahumahi.

Manatoko Motuhake

Whakamātautau whakataetae: Ka aromatawaihia te kaha o te aptamer ki te here ki tētahi ūnga motuhake i te aroaro o ētahi atu ūnga rite. Mena ka taea e te aptamer te here motuhake ki te ngota ūnga me te kore e pokanoa mai i ētahi atu ngota, he tohu tēnei he tino motuhake.

Whakamātautau tauhohenga whakawhiti: Ka herea te aptamer ki tētahi raupapa o ngā ūnga e pā ana, e kore rānei e pā ana, kia kitea ai mēnā ka herea noa ki tētahi ūnga motuhake, kia manatokohia ai tōna motuhaketanga.

Manatoko pumau

Whakamātautau whakapōrearea nuclease: I tukuna ngā aptamer ki ngā kukū rerekē o ngā nuclease, ā, i kitea tō rātou whakapōrearea. Mā te whakatairite i te nui o te whakapōrearea i ngā wā rerekē, ka taea te aromatawai i te kaha whakapōrearea anti-nuclease o te aptamer.

Whakamātautau pumau pāmahana me te wā: I whakanohoia te aptamer ki raro i ngā āhuatanga pāmahana me te wā rerekē hei tirotiro i tōna pumau hanganga me te mahi. Mā tēnei ka āwhina ki te whakatau i ngā āhuatanga rokiroki me te whakamahinga pai rawa atu mō ngā aptamer.

Te tautuhi i ngā mahi koiora

Ko te tikanga tika ka tīpakohia i runga anō i te whāinga motuhake o te aptamer waikawa karihi.

(1) Whakamātautau taumata ngota: Mā te whakamahi i ngā tikanga koiora ngota, pērā i te electrophoresis gel, Western blot, me ētahi atu, hei kimi i ngā matatini i hangaia i muri i te hanumitanga o te aptamer ki te ngota ūnga, hei kimi rānei i ngā huringa o te taumata whakapuakanga o te ngota ūnga i puta mai i te aptamer.

(2) Whakamātautau taumata pūtau: Mā te whakamahi i te hangarau whakatipu pūtau, ka whakatōkia ngā aptamers ki ngā pūtau ūnga hei tirotiro i ngā huringa koiora pēnei i te āhua o te pūtau, te tipu me te apoptosis, me te aromatawai i te mahi koiora o ngā aptamers.

(3) Whakamātautau tauira kararehe: I roto i ngā tauira kararehe e tika ana, ka tukuna ngā aptamers waikawa karihi mā te werohanga, mā te whakahaere rongoā rānei, ā, ka tirohia ngā tohu ā-tinana me ngā huringa mate o ngā kararehe hei aromatawai i te mahi koiora me te haumaru o ngā aptamers i roto i te tinana ora.