Pūhara Whakawhanake Aptamer

Ko ngā Aptamers he oligonucleotide kotahi-aho (DNA, RNA, XNA rānei) he āhuatanga tino kaha te piripono me te tino motuhake e herea ana ki ngā ngota whainga pēnei i ngā paturopi, ā, e whakamahia whānuitia ana mō te whakawhanake i ngā pūoko koiora, te tautuhi me te rongoā.

Ko te tūāpapa aptamer e whakaratohia ana e Alpha Lifetech e rua ngā wāhanga: te tūāpapa whakahiato aptamer, e uru ana ki te ratonga whakahiato whare pukapuka aptamer a SELEX me te ratonga whakawhanake aptamer (DNA, RNA, XNA rānei), me te tūāpapa tirotiro aptamer tae atu ki ngā ratonga tirotiro e hangai ana ki te hangarau SELEX mō ngā pūmua, ngā peptide, ngā pūtau, ngā ngota iti, ngā katote konganuku me ētahi atu ngota ūnga, tae atu ki ngā ratonga arotakenga aptamer me te tātari tautuhi.

Pūhara Hanganga Aptamer

Ratonga whakahiato whare pukapuka aptamer SELEX

Ko te ratonga whakahiato whare pukapuka aptamer SELEX ko te hanga i tētahi whare pukapuka kei roto he maha o ngā raupapatanga oligonucleotide kotahi-aho matapōkere mā te whakahiato matū in vitro e ai ki ngā ngota ngota e whai ana. Ko te hanganga whare pukapuka te tīmatanga o te hangarau SELEX, e whakarato ana i ngā raupapatanga kaitono maha mō te tukanga tātaritanga e whai ake nei mā te hanga whare pukapuka matapōkere nui, me te whakanui ake i te taea o te tātari i ngā aptamer hononga-teitei.

Ko te whakahiato whare pukapuka ka wehea ki ngā mahi e whai ake nei:

Ngā Hipanga	Ngā Taipitopito Hangarau
Tāutuhia ngā ngota whāinga	Tāutuhia ngā ngota whainga e tika ana kia tirotirohia mō ngā aptamers, pēnei i ngā pūmua, ngā waikawa karihi, ngā ngota iti, ngā katote konganuku, me ētahi atu.
Hoahoa Raupapa Matapōkere	I hangaia te roa o te raupapatanga matapōkere, te hanganga turanga me ētahi atu tawhā i runga anō i ngā āhuatanga o ngā ngota whainga me ngā whakaritenga tātari. I te nuinga o te wā, ko ngā raupapatanga matapōkere kei waenganui i ngā tekau me ngā rau o ngā turanga te roa.
Te Hanganga o ngā Raupapa Pūmau	Ka hoahoatia, ka whakahiatohia hoki ngā maramara oligonucleotide me ngā raupapatanga pumau (pērā i ngā raupapatanga PCR primer) i ngā pito e rua, ā, ka whakamahia ēnei i roto i te tukanga whakanui me te tātaritanga e whai ake nei.

Me tukatuka tonu te whare pukapuka kua whakahiatohia kia pai ai te whakahaere kounga. I whakatauhia te kukū o te whare pukapuka kia pai ai te whakamahi i roto i te tukanga tātari i muri mai. I manatokohia te kanorau me te tika o ngā raupapatanga matapōkere i roto i te whare pukapuka mā te raupapatanga me ētahi atu tikanga kia tutuki ai ngā whakaritenga tātari i te kounga o te whare pukapuka.

Mā ngā mahi o runga ake nei, ka taea te hanga i tētahi whare pukapuka aptamer SELEX kounga teitei, he tino kanorau hoki, ka taea ai te whakarato i ngā raupapatanga kaitono maha mō te tukanga tātaritanga e whai ake nei.

Ngā ratonga whakawhanake Aptamer (DNA, RNA, XNA rānei)

Ko ngā aptamers e pā ana ki ngā aptamers waikawa karihi. Kei roto i ngā aptamers waikawa karihi ngā aptamers DNA, ngā aptamers RNA, me ngā aptamers XNA, arā, he aptamers waikawa karihi kua whakarerekētia e te matū. He whānuitia te whakamahinga o te tikanga SELEX mō te whakawhanaketanga o ngā aptamers. Ko te tukanga mahi taketake o ngā ratonga whakawhanake aptamers ko te hanga whare pukapuka, te herea ūnga, te wehe me te purenga, te whakanui, ngā rauna tirotiro maha, me te tautuhi raupapatanga. Mō ngā tau maha, kua arotahi mātou ki te hanga whare pukapuka me te wheako nui ki te whakawhanaketanga aptamers. E whakapau kaha ana mātou ki te whakarato ratonga pai ake ki ngā kiritaki.

Tukanga Hangarau SELEX

He maha ngā wāhanga o te tukanga SELEX, ā, kei roto i ia wāhanga ngā mahi matua e whai ake nei:

Te Herenga Whare Pukapuka me te Herenga Whāinga

Ka konatunatua te whare pukapuka waikawa karihi kua hangaia ki ngā ngota whainga motuhake (pērā i ngā pūmua, ngā matū ngota iti, me ētahi atu), kia whai wāhi ai ngā raupapatanga waikawa karihi i roto i te whare pukapuka ki te here ki ngā ngota whainga.

Te Wehewehenga o ngā Ngota Korehere

Ko ngā raupapatanga waikawa karihi kāore e herea ki te ngota ūnga ka wehea mai i te ranunga mā roto i ngā tikanga motuhake pērā i te chromatography hononga, te wehenga pirepire aukume, me ētahi atu.

Te Whakarei ake i ngā Ngota Here

Ka whakanuia te raupapatanga waikawa karihi e herea ana ki tētahi ngota ūnga, mā te whakamahi i te hangarau tauhohenga mekameka polymerase (PCR). Mō te wāhanga tirotiro e whai ake nei, ka whakamahia ngā raupapatanga kua whakanuia hei whare pukapuka tīmatanga.

Pikitia 1: Te tukanga tirotiro SELEX

Pūhara Tirotiro Aptamer

Ratonga tirotiro Aptamer

He whānuitanga o ngā ratonga tirotiro aptamer motuhake a Alpha Lifetech e whakamahi ana i ngā tikanga SELEX mō ngā momo rerekē o ō ngota ngota:

Ngā Momo Whāinga	Ngā Taipitopito Hangarau
Te Tirotiro Aptamer Pūmua nā SELEX	Ko te kaupapa matua o te tirotiro i ngā aptamer pūmua he tirotiro i ngā aptamer ka taea te here motuhake ki ngā ngota pūmua ūnga. He māmā ake te hanga i ēnei aptamer, he pumau ake, ā, he iti ake te pāngia e ngā āhuatanga taiao.
Te Tirotiro Peptide Aptamer nā SELEX	Ko ngā peptide aptamers he akomanga o ngā raupapatanga peptide poto he tino motuhake me te kaha ki te hono, ka taea te here motuhake ki ngā matū ūnga me te whakaatu i te whānuitanga o te pūmanawa tono i roto i te mara hauora. Mā roto i tētahi tukanga tātaritanga motuhake, ka tātarihia ngā peptide aptamers ka taea te here motuhake ki ngā ngota ūnga mai i te maha o ngā whare pukapuka raupapatanga peptide matapōkere.
Te Tirotiro Aptamer Motuhake-Pūtau (Cell-SELEX)	Ka whakaritea ngā pūtau ūnga, ngā ngota motuhake rānei i runga i te mata o te pūtau hei ūnga. Ka taea e ngā ūnga te noho hei pūtau katoa, hei kaiwhakawhiwhi i runga i te kiriuhi pūtau, hei pūmua, hei ngota iti rānei.
Te Tirotiro i te Aptamer Molecule Iti nā Capture SELEX	He tikanga tirotiro in vitro a Capture SELEX mō te tirotiro i ngā aptamers ngota iti, he momo rerekē tēnei o SELEX. He tino pai a Capture SELEX mō te tirotiro aptamer i ngā ūnga ngota iti, he iti ake ngā rōpū mahi, ā, he uaua ki te whakaū tika ki runga i ngā tautoko ā-wāhanga totoka.
Ngā ratonga SELEX ora e hangai ana ki ngā kararehe	Ko te ratonga tirotiro kararehe ora he tikanga whakamātautau e whakamahia whānuitia ana i roto i ngā mara o te pūtaiao koiora, te rongoā me te hangarau koiora, e whakamahi ana i ngā kararehe ora hei tauira whakamātautau hei tirotiro me te aromatawai i ngā ngota motuhake, te rata, te rongoā, ngā tukanga koiora rānei. Kua hangaia ngā ratonga hei whakatauira i te taiao ā-tinana i roto i te tinana tangata kia tika ake ai te matapae me te aromatawai i te whai huatanga me te haumaru o ngā hua whakamātautau i roto i te tinana tangata.

Ratonga arotau Aptamer

Nā te kaha ki te wai, te ngaronga o te kaha ki te hanga, me te tere o te tango i ngā aptamers, ka whakawhāitihia te whakamahinga. I tēnei wā, he maha ngā tikanga arotau kua tūhuratia hei whakarei ake i te mahi a ngā aptamers.

He maha ā mātou huarahi hei arotau i te aptamer, arā, te tapahi, te whakarerekētanga, te honohono ki te rōpū e tika ana (thiol, carboxy, amine, fluorophore, me ētahi atu).

Ratonga tātaritanga āhuatanga Aptamer

Ko te ratonga tātari āhuahanga Aptamer e pā ana ki te ratonga ngaio mō te whakatau hanganga aromatawai mahi me te manatoko mahi o te aptamer kua whiwhihia hei whakarite kia tutuki i te aptamer ngā whakaritenga mō te kaha here, te pumau me te motuhaketanga. Kei roto i tēnei ko te tātari hononga me te motuhaketanga, te aromatawai pumau me te manatoko mahi koiora.