Pūhara Hangarau Hybridoma

He nui ngā wheako o Alpha Lifetech i roto i te ao o te whakawhanaketanga paturopi. I roto i ngā tau, kua whakatūria e Alpha Lifetech tētahi tūāpapa whakawhanaketanga paturopi whānui (tae atu ki te whakawhanaketanga paturopi monoclonal, te whakawhanaketanga paturopi polyclonal, te whakawhanaketanga paturopi monoclonal recombinant, me ētahi atu ratonga whakarite me te whakawhanaketanga paturopi).

Ka whakaratohia e Alpha Lifetech ki ngā kiritaki he hua me ngā ratonga paturopi me te paturopi recombinant kua whakapūmautia te kounga, ā, he kaha te motuhaketanga me te pumau o ngā paturopi kua oti te whakarite. Ka taea te whakamahi i ngā taputapu me ngā taputapu purenga paturopi maha hei whakarato ratonga purenga paturopi mai i ngā momo pūtake pēnei i te rāpeti, te hipi, te heihei, te kiore monoclonal paturopi, tae atu ki ngā ratonga purenga hononga pūmua A/G me ngā ratonga wehenga paturopi.

I runga i ngā tikanga whakarite paturopi maha pērā i te hangarau pūtau hybridoma, ka taea e Alpha Lifetech te whakarato ratonga ritenga mō ngā paturopi monoclonal me polyclonal o ngā momo rerekē. Hei tāpiri, ka taea hoki te whakarato i te whānuitanga o ngā ratonga mai i te raupapatanga tere teitei, te whakarite paturopi, te purenga paturopi ki te whakamana paturopi. Ka whiriwhiria e mātou te tikanga purenga i runga i te kaupapa motuhake a te kiritaki. I runga i ngā pūnaha tūāpapa whānui pērā i te tūāpapa whakawhanake paturopi me te tūāpapa pūmua, ka hipokina e Alpha Lifetech ngā ratonga whakarunga me te whakararo o te hanga paturopi, te whakarato i te purenga paturopi, te raupapatanga tere teitei paturopi, te whakamana paturopi me ētahi atu ratonga hangarau.

Hangarau Hybridoma

Ka taea te whakamahi i te hangarau Hybridoma hei whakarite i ngā paturopi monoclonal. Mā te whakakotahi i ngā pūtau whēkau mai i ngā kiore kua werohia ki ngā pūtau myeloma mai i te tangata, i ngā kiore rānei, ka hangaia ngā pūtau hybridoma ka huna i ngā paturopi motuhake. Ka taea te whakamahi i ngā paturopi monoclonal i hangaia mai i te kiore i roto i ngā rangahau pūtaiao pēnei i te whakamātautau immunosorbent enzyme-linked, radioimmunoassay, immunohistochemistry, flow cytometry, purenga pūmua, me ētahi atu. Kei roto i ngā tauira paturopi monoclonal ko te ascites, te supernatant hybridoma, me ētahi atu. Ko te tauira i whiwhihia mā te werohanga i ngā pūtau hybridoma ki roto i te kōhao puku o ngā kararehe me te whakamahi i te taiao ā-tinana o te kōhao puku o te kararehe hei whakaputa paturopi ka kiia ko te tauira ascites. Ka taea e ngā pūtau hybridoma i werohangahia ki roto i te kōhao puku o ngā kararehe te tipu me te huna i ngā paturopi monoclonal, ka taea te kohikohi i roto i ngā ascites. Nā te mea ka taea te noho o ngā poke (ngā pūmua kararehe, ngā homoni, me ētahi atu) i roto i ēnei paturopi, me purenga. I runga i te mahi huna o ngā lymphocytes B, ka huna pinepinetia ngā paturopi monoclonal ki roto i te reo ahurea i te wā e whakatipu ana i ngā pūtau hybridoma. Ko te wai kei roto ngā paturopi monoclonal i roto i te reo ahurea ka kiia ko te supernatant hybridoma. He tino teitei te parakore o ngā paturopi monoclonal i roto i te waikura, engari me whakapai ake te purenga mō te whakaritenga nui nā te mea ka riro mai mā te ahurea in vitro.

Ka taea e te purenga paturopi te wehe i ngā paturopi parakore-teitei mai i ngā matatini. I tēnei wā, ko te purenga hononga paturopi me te wehenga me te purenga paturopi ngā tikanga purenga paturopi e whakamahia whānuitia ana, tae atu ki te purenga pūmua A/G, te chromatography whakawhiti katote, te tātari ira, te chromatography tauhohenga waipēhi me te chromatography taunekeneke waipēhi. I roto i ēnei, he rongonui te ratonga purenga hononga pūmua A/G i roto i ngā tono purenga paturopi. Ko te tukanga a Alpha Lifetech e whakarite ana i ngā paturopi monoclonal i runga i te hangarau hybridoma e whakaaturia ana i te Pikitia 1.

Pikitia 1. Kauwhata tukanga mō te whakarite i ngā paturopi monoclonal i runga i te hangarau hybridoma.

Hangarau Hononga Rongoā Paturopi me te Hangarau Hybridoma

Ko te hononga rongoā paturopi (ADC) he matū koiora hou e hono ana i ngā paturopi ki ngā rongoā paitini. Ka whakakotahi te hononga rongoā paturopi i te kaha ki te mōhio motuhake o ngā paturopi me te pānga whakamate kaha o ngā rongoā paitini, ā, ka tukuna tika ngā rongoā paitini ki roto i ngā pūtau pukupuku mā roto i ngā paturopi, ā, ka tutuki te maimoatanga tika. Ko te wāhanga paturopi i roto i ngā rongoā ADC ka whakaritea mā te hangarau hybridoma, mā ētahi atu tikanga whakaputa paturopi rānei, pērā i te hangarau paturopi hangarau ira. Ka whakaratohia e te hangarau hybridoma he rauemi mata paturopi kounga teitei mō ngā rongoā ADC. He mahi nui tā ngā rongoā ADC me ngā paturopi monoclonal i whakaritea mā te hangarau hybridoma i roto i ngā mara pērā i te maimoatanga mate pukupuku. Ka taea te whakamahi takitahi, te whakamahi tahi rānei hei whakarei ake i te whai huatanga o te rongoā.

He tino motuhake ngā paturopi monoclonal i hangaia mā te hangarau hybridoma, ka taea te here tika me te kite i ngā antigens motuhake, ā, ka taea te whakaputa i runga i te tauine nui. Mā te hangarau raupapatanga hybridoma, ka taea te tango me te raupapa i ngā ira paturopi i roto i ngā pūtau hybridoma kia whiwhi ai i ngā raupapatanga e rite ana, he mea pai mō te whakaaturanga paturopi i muri mai. Ka taea te whakamahi i ngā paturopi monoclonal i hangaia mā te hangarau hybridoma hei kite i ngā tohu mate motuhake, pērā i ngā tohu pukupuku, ngā antigens huaketo, me ētahi atu, e whakarato ana i te tautoko kaha mō te tautuhi wawe i ngā mate. Hei tāpiri, ka taea hoki te whakamahi i roto i ngā whakamātautau pērā i te immunoblotting me te immunohistochemistry hei ako i te whakaaturanga pūmua, te rohe, te hanganga, me te mahi.

hangarau hybridoma Rerenga Mahi Ratonga Paturopi Whakaputa

Ngā Hipanga	Ihirangi Ratonga	Rārangi Wā
Hipanga 1: Te werohanga ārai mate mō ngā kararehe	(1) 4 ngā werohanga ārai mate mō ngā kararehe, 1 te horopeta ārai mate tāpiri, e 5 katoa ngā werohanga i werohangatia. (2) I mua i te kohi i te werohanga, i puta te hua kino o te toto, ā, i mahia te ELISA i te tuawhā o ngā horopeta hei kite i te taumata o te toto. (3) Mena ka tutuki i te taumata o te paturopi toto o te tuawhā o ngā horopeta ngā whakaritenga, ka tukuna tētahi atu horopeta ārai mate i ngā rā e 7 i mua i te kohikohinga toto. Mena kāore e tutuki i ngā whakaritenga, ka haere tonu te ārai mate noa. (4) He kaha kua tohua, ka wehea ngā pūtau whatukuhu mō te whakakotahitanga pūtau.	10-12 wiki
Hipanga 2: Te Whakakotahitanga me te Tirotiro	(1) Hononga: Whai muri i te werohanga, ka kohia ngā pūtau whēkau kiore, ā, ka honoa ki ngā pūtau myeloma. (2) Te whakatipu me te tirotiro: Ko ngā pūtau hybridoma i whiwhihia i tukuna ki te tirotiro whakatipu me te tirotiro ELISA kia whiwhi ai i ngā tāruarua tino whai hua.	6 wiki
Hipanga 3: Tāruarua	Te whakarōpū i ngā pūtau whaea pai 15-25 mā te whakamahi i te tikanga whakawhāititanga iti, ā, hei whakamutunga, te whakatau i ngā whakarōpū pai 10-15.	3-4 wiki
Hipanga 4: Te Hanga Ascites me te Whakamaemaetanga o te Paturopi	(1) Te whakaputanga o te ascites: 10-15 ngā momo hybridoma e whakamahia ana mō te whakaputanga o te ascites i roto i ngā kiore (mēnā he nui ngā momo hybridoma ka riro mai, ka tukuna atu i roto i te āhua pūtau) (2) Te tautuhi i ngā ascites: Te kitenga ascites ELISA, te kaha (pūmua/huaketo>10 ^ 5, ngota iti/peptide>10 ^ 4) mō te purenga o muri (3) Te Whakamaemaetanga o te Pūmua A/G: Te Whakamaemaetanga o ngā Ascites	3-4 wiki
Hipanga 5: Tukunga	(1) Whakaratohia kia 3-5 ngā momo paturopi monoclonal hybridoma. (2) Ka whakaratohia e ia pūtau hybridoma te 1mg o te paturopi parakore (te purenga o te ascites). (3) Pūrongo Tukanga Whakamātautau	1 wiki

Painga o te Hangarau Hybridoma

He nui ngā wheako o Alpha Lifetech i roto i te ao whakawhanake paturopi. I roto i ngā tau, kua whakatūria e Alpha Lifetech tētahi tūāpapa whakawhanake paturopi whānui.

Ngā Momo Maha

Ka taea te whakarato i te ārai mate ki ngā momo kararehe, tae atu ki ngā kiore, ngā rāpeti, ngā hipi, me ētahi atu, engari kāore i te herea ki tēnā.

Whāinga Maha

I runga i te hangarau hybridoma, he maha ngā ratonga kimi paturopi ūnga e wātea ana: ngā pūmua, ngā peptide, ngā ngota iti, ngā huaketo, ngā pūmua kiriuhi, te mRNA, me ētahi atu.

Ngā Rekoata Whai Aroturuki

Ngā paerewa whakahaere kounga QC (te kaha o te ārai mate, te whakahaere kounga PBMC, te whakahaere kounga whare pukapuka, me te whakahaere kounga whakamana tirotiro), ngā pūrongo whakamātautau Ingarihi, ngā tuhinga whakamātautau taketake

Kaupapa Whaiaro

Whakaritenga kaupapa takitahi hei whakatutuki i ngā hiahia kaupapa rangahau o ngā kiritaki rerekē

RATONGA E Pā Ana

Ngā Rautaki Whakawhanaketanga Paturopi Maha

Ratonga Whakawhanake Paturopi Monoclonal

Pūhara Hangarau Hybridoma

Pūhara Tātari Pūtau B Kotahi

Pūhara Whakawhanake Paturopi Whakaaturanga Phage

te whanaketanga o te paturopi monoclonal-Alpha Lifetech

Ratonga Whakawhanake Paturopi Monoclonal

Ka taea e mātou te whakarato ratonga whakawhanake paturopi monoclonal kounga teitei, tino parakore, me te tino motuhake, tae atu ki te hanga paturopi monoclonal kiore me ngā paturopi monoclonal rāpeti.